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Immobilization Of Enzymes And Cells
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Book Synopsis Immobilization of Enzymes and Cells by : Jose M. Guisan
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2016-08-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Book Synopsis Immobilization of Enzymes and Cells by : Jose M. Guisan
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2021-01-29 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Book Synopsis Immobilization of Enzymes and Cells by : José M. Guisán
Download or read book Immobilization of Enzymes and Cells written by José M. Guisán and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
Book Synopsis Immobilized Biocatalysts by : Winfried Hartmeier
Download or read book Immobilized Biocatalysts written by Winfried Hartmeier and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.
Book Synopsis Immobilization of Enzymes and Cells by : Gordon Bickerstaff
Download or read book Immobilization of Enzymes and Cells written by Gordon Bickerstaff and published by Humana Press. This book was released on 1996-12-01 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.
Book Synopsis Immobilization of Cells by : Colin R. Phillips
Download or read book Immobilization of Cells written by Colin R. Phillips and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Growth of immobilized cells can be viewed as an alternative to growth of free cells in many instances. In others, immobilization confers a precision of control over the process not possible in free growth. Immobilization of cells can sometimes be considered to be a lower cost alternative to immobilization of enzymes. In this volume, immobilization procedures based on mechanical means and bonding of various types are examined, with detailed application examples. These applications include microorganisms, plant and animal cells, sub-cellular organelles and multiple enzyme systems. Particular attention is devoted to enzyme properties in immobilized cells and the properties of the carrier. The volume should provide the reader with a comprehensive overview of the subject, together with copious references. As well as serving as a research monograph, it could be used to provide reference material for a graduate course. Special thanks are due Mrs. JENNIFER KERBY for her dedicated work in the preparation of the manuscript, and IT-CHIN HSIEH for bibliographical assistance. COLIN R. PHILLIPS Toronto, July 1988 YIU C. POON v Table of Contents 1 Introduction. 1 References . 9 2 Methods of Cell Immobilization 11 2.1 Mechanical Immobilization . 11 2.1.1 Mycelial Pellet and Mat 11 2.1.2 Encapsulation .. 48 2.1.3 Dialysis Culture. . . 49 2.1.4 Entrapment. .... 50 2.2 Covalent Attachment 61 2.3 Ionic Attachment 62 2.3.1 Flocculation 62 2.3.2 Adsorption . 64 References . 66 3 Special Problems and Extended Applications . 75 3.1 Special Problems and Techniques .
Book Synopsis Immobilized Biosystems by : I.A. Veliky
Download or read book Immobilized Biosystems written by I.A. Veliky and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of the immobilization of viable and non-viable cells, proteins, enzymes and active molecules, and their interaction with natural or synthetic carriers for performing biochemical and chemical reactions in vivo and in vitro.
Book Synopsis Fundamentals of Cell Immobilisation Biotechnology by : Viktor Nedovic
Download or read book Fundamentals of Cell Immobilisation Biotechnology written by Viktor Nedovic and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.
Book Synopsis Advances in Food Biotechnology by : Ravishankar Rai V
Download or read book Advances in Food Biotechnology written by Ravishankar Rai V and published by John Wiley & Sons. This book was released on 2015-12-21 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of biotechnology in the food sciences has led to an increase in food production and enhanced the quality and safety of food. Food biotechnology is a dynamic field and the continual progress and advances have not only dealt effectively with issues related to food security but also augmented the nutritional and health aspects of food. Advances in Food Biotechnology provides an overview of the latest development in food biotechnology as it relates to safety, quality and security. The seven sections of the book are multidisciplinary and cover the following topics: GMOs and food security issues Applications of enzymes in food processing Fermentation technology Functional food and nutraceuticals Valorization of food waste Detection and control of foodborne pathogens Emerging techniques in food processing Bringing together experts drawn from around the world, the book is a comprehensive reference in the most progressive field of food science and will be of interest to professionals, scientists and academics in the food and biotech industries. The book will be highly resourceful to governmental research and regulatory agencies and those who are studying and teaching food biotechnology.
Book Synopsis Enzymes and Immobilized Cells in Biotechnology by : Allen I. Laskin
Download or read book Enzymes and Immobilized Cells in Biotechnology written by Allen I. Laskin and published by Butterworth-Heinemann. This book was released on 1985 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Immobilization of Enzymes and Cells by : Jose M. Guisan
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2020-01-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Book Synopsis Immobilized Microbial Cells by : Ichiro Chibata
Download or read book Immobilized Microbial Cells written by Ichiro Chibata and published by Elsevier. This book was released on 2014-06-28 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Book Synopsis Cell Encapsulation Technology and Therapeutics by : Willem_M. Kühtreiber
Download or read book Cell Encapsulation Technology and Therapeutics written by Willem_M. Kühtreiber and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept of using encapsulation for the immunoprotection of transplanted cells was introduced for the first time in the 1960s. "[Microencapsulated cells] might be protected from destruction and from partici pation in immunological processes, while the enclosing membrane would be permeable to small molecules of specific cellular product which could then enter the general extracellular compartment of the recipient. For instance, encapsulated endocrine cells might survive and maintain an effective supply of hormone." (Chang, Ph. D. Thesis, McGill University, 1965; Chang et aI., Can J Physiol PharmacoI44:115-128, 1966). We asked Connaught Laboratories, Ltd., in Toronto to put this concept into practice. In 1980, Lim and Sun from Connaught Laboratories reported on the successful implantation of poly-I-Iysine-alginate encapsu lated rat islets into a foreign host. [Lim and Sun, Science 210:908-909, 1980]. Now many groups around the world are making tremendous progress in the encapsulation of a multitude of cell types. Kiihtreiber, Lanza, and Chick have invited many cell encapsulation groups from around the world to contribute to this book. The result is a very useful reference book in this rapidly growing area. With so many excellent au thors describing in detail the different areas of cell encapsulation, my role here will be to briefly discuss a few points.
Book Synopsis Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives by : Jesús Fernández Lucas
Download or read book Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives written by Jesús Fernández Lucas and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of innovative tools for creative nucleic acid chemists that open the door to novel probes and therapeutic agents Nucleic acids continue to gain importance as novel diagnostic and therapeutic agents. With contributions from noted scientists and scholars, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practical reference that includes a wide range of approaches for the synthesis of designer nucleic acids and their derivatives. The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications. This important book: -Presents a timely and topical guide to the synthesis of designer nucleic acids and their derivatives -Addresses the growing market for nucleotide-derived pharmaceuticals used as anti-infectives and chemotherapeutic agents, as well as fungicides and other agrochemicals. -Covers novel methods and the most recent trends in the field -Contains contributions from an international panel of noted scientistics Written for biochemists, medicinal chemists, natural products chemists, organic chemists, and biotechnologists, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practice-oriented guide that reviews innovative methods for the enzymatic as well as non-enzymatic synthesis of nucleic acid species.
Book Synopsis Bioaffinity Chromatography by : J. Turková
Download or read book Bioaffinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1993-09-14 with total page 799 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography. Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.
Book Synopsis Applications of Cell Immobilisation Biotechnology by : Viktor Nedovic
Download or read book Applications of Cell Immobilisation Biotechnology written by Viktor Nedovic and published by Springer Science & Business Media. This book was released on 2006-04-06 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell immobilisation biotechnology is a multidisciplinary area, shown to have an important impact on many scientific subdisciplines – including biomedicine, pharmacology, cosmetology, food and agricultural sciences, beverage production, industrial waste treatment, analytical applications, biologics production. "Cell Immobilisation Biotechnology" is an outcome of the editors’ intention to collate the extensive and widespread information on fundamental aspects and applications of immobilisation/encapsulation biotechnology into a comprehensive reference work and to provide an overview of the most recent results and developments in this domain. "Cell Immobilisation Biotechnology" is divided into the two book volumes, FOBI 8A and FOBI 8B. The FOBI 8A volume, Fundamentals of Cell Immobilisation Biotechnology, is dedicated to fundamental aspects of cell immobilisation while the present volume, FOBI 8B, Applications of Cell Immobilisation Biotechnology, deals with diverse applications of this technology.
Book Synopsis Biocatalysts and Enzyme Technology by : Klaus Buchholz
Download or read book Biocatalysts and Enzyme Technology written by Klaus Buchholz and published by John Wiley & Sons. This book was released on 2012-12-21 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added. Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery, characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered.
