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Protein And Peptide Based Microarrays For Multiplex Detection
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Book Synopsis Protein and Peptide-based Microarrays for Multiplex Detection by : Navid Rabiee
Download or read book Protein and Peptide-based Microarrays for Multiplex Detection written by Navid Rabiee and published by IOP Publishing Limited. This book was released on 2020-10-07 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein and Peptide-based Microarrays for Multiplex Detection looks at the aspects of protein and peptide-based microarrays for multiplex detection, based on Rabiee's Theory. A comprehensive guide for scientists, students and doctors.
Book Synopsis Evolution of Whole Protein and Peptide Microarray Platforms for Biological Discovery and Personalized Medicine by : Jordan Vail Price
Download or read book Evolution of Whole Protein and Peptide Microarray Platforms for Biological Discovery and Personalized Medicine written by Jordan Vail Price and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the advent of microarray technology in the early 1990's, the development of highly multiplex genomics tools has greatly outpaced the field of proteomics in terms of the generation of useful platforms for basic research and clinical analysis. Yet, to deliver on the great promise of genomics tools that analyze nucleic acids, technologies that permit the analysis of protein-protein interactions on a comparable scale need to be developed. Our group and others have shown that whole-protein and peptide microarrays can provide large amounts of information about the protein interactions that underlie the human immune response in health and disease. Here we describe three novel proteomics microarray platforms and demonstrate that the arrays can be used to gain insight into the antibody-mediated immune response in a spectrum of human diseases. In the first study, we detail the generation of a protein microarray that allows the detection of antibodies that bind to the molecular communication factors of the immune system: cytokines, chemokines, growth factors, and other circulating proteins. Using these arrays, we identified novel targets of autoantibodies in systemic lupus erythematosus, which may delineate a unique subgroup of patients within the spectrum of lupus. In the second study, we describe influenza antigen microarrays that permitted the identification of antibody reactivity to specific influenza hemagglutinin epitopes associated with poor or effective response to the seasonal influenza vaccine. These arrays have potential for development into a clinical diagnostic tool capable of predicting an individual's ability to mount an effective antibody response to vaccination. Finally, we report the development of a silicon-based peptide microarray developed in collaboration with researchers at the Intel Corporation. These "Intel arrays" hold great potential for ultimately enabling highly parallel, real-time measurement of protein-protein interaction with myriad possible downstream applications. The technologies we have developed move the field of multiplex proteomic analysis forward on many fronts, and constitute valuable additions to the proteomics toolkit available to researchers and clinicians. Moreover, our work demonstrates that detailed analysis of protein-protein interaction is required to make more informed models of different states of human health and disease.
Book Synopsis Peptide Microarrays by : Marina Cretich
Download or read book Peptide Microarrays written by Marina Cretich and published by Springer Nature. This book was released on 2022-09-24 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and more accessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms.
Download or read book Rapid Test written by Laura Anfossi and published by BoD – Books on Demand. This book was released on 2018-09-26 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid tests, also known as point-of-care tests, have been in use for decades in the clinical and medical area and have become increasingly popular as an efficient screening method for conducting on-site analysis thanks to their simplicity, speed, specificity and sensitivity. Nowadays, rapid tests are widely applied for clinical, drug, food, forensic and environmental analysis and fields of application are rapidly increasing together with advances in the technology. The growing interest in rapid tests and their expanding application in diverse fields, together with requirements of improved sensitivity, reliability, multiple detection capacity and robustness, are prompting innovation in the design of novel platforms, and in the exploitation of innovative detection strategies. The book covers advances in materials, technology and test design.
Book Synopsis Protein Microarray Technology by : Dev Kambhampati
Download or read book Protein Microarray Technology written by Dev Kambhampati and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins. The list of authors consisting of world leading experts provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions. Topics covered include: -Types of biomolecular interactions -Surface chemistry -Detection technologies -Spotting technologies -Bioinformatics/data analysis. While primarily intended to serve as a reference for researchers and students embarking on the exciting fields of proteomics, drug discovery and clinical diagnostics, this technology is also expected to potentially impact the areas of food diagnostics, environmental monitoring and national security.
Book Synopsis Applying Genomic and Proteomic Microarray Technology in Drug Discovery, Second Edition by : Robert S. Matson
Download or read book Applying Genomic and Proteomic Microarray Technology in Drug Discovery, Second Edition written by Robert S. Matson and published by CRC Press. This book was released on 2013-03-13 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarrays play an increasingly significant role in drug discovery. The commercial landscape has changed dramatically over the past few years and researchers have made great advancements with regard to construction and use. Now in its second edition, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. Updated and revised to reflect recent progress in the field, the second edition discusses: Expanded omics-driven applications, including the areas of metabolomics and chemical biology The commercialization of the microarray platform, with a historical perspective aimed at recognizing key technological developments Solid-supports (substrates) and surface chemistries currently used in the creation of nucleic acid and protein microarrays Different approaches to producing microarrays that achieve spot equality with the same number of molecules properly oriented The development of the gene expression microarray and representative applications The development of protein microarray technology, including its history and key applications Unique to this edition is a new chapter on multiplex assays that examines the development and applications of arrays across diverse platforms. It discusses applications for qPCR, multiplex lateral flow, and multiplex bead assays. It also presents platform-to-platform comparisons. Microarrays remain an invaluable tool for omics-based research not only in drug discovery, but in the life sciences, in clinical research, and for diagnostic applications worldwide. This volume presents the current state of the art on the utility of this technology to solve a host of important biological problems.
Download or read book Microarrays written by Kilian Dill and published by Springer Science & Business Media. This book was released on 2008-12-30 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combinatorial chemistry is used to find materials that form sensor microarrays. This book discusses the fundamentals, and then proceeds to the many applications of microarrays, from measuring gene expression (DNA microarrays) to protein-protein interactions, peptide chemistry, carbodhydrate chemistry, electrochemical detection, and microfluidics.
Book Synopsis Microarrays in Diagnostics and Biomarker Development by : Bertrand Jordan
Download or read book Microarrays in Diagnostics and Biomarker Development written by Bertrand Jordan and published by Springer Science & Business Media. This book was released on 2012-04-23 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards “nanoarrays”, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.
Book Synopsis Protein Microarrays by : Mark Schena
Download or read book Protein Microarrays written by Mark Schena and published by Jones & Bartlett Learning. This book was released on 2005 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Microarrays is one of the first books in this exciting new technology and will be an essential reference for most biologists and biotechnology professionals.
Book Synopsis Microarrays and Microplates by : Ian Day
Download or read book Microarrays and Microplates written by Ian Day and published by Taylor & Francis. This book was released on 2023-05-31 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 'Advanced Methods' series is intended for advanced undergraduates, postgraduates and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems. Microarrays and Microplates title looks at the new microarray and microplate-based technologies which facilitate large-scale analysis of DNA sequence variants, mRNA molecules and proteins. The book provides a review of the various methodologies being used to identify genetic variants and gene regulation and guides readers through both the application of these methodologies and experimental procedures.
Book Synopsis Multiplex Microarrays for Diagnosis and Detection by : Bradley H. Garcia
Download or read book Multiplex Microarrays for Diagnosis and Detection written by Bradley H. Garcia and published by . This book was released on 2008 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Functional Protein Microarrays in Drug Discovery by : Paul F. Predki
Download or read book Functional Protein Microarrays in Drug Discovery written by Paul F. Predki and published by CRC Press. This book was released on 2007-03-30 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: An ideal text for biotechnologists, protein chemists, and biochemists, Functional Protein Microarrays in Drug Discovery explores all aspects of functional protein microarrays, including basic principles, methods, and applications. The book discusses the generation of functional protein content and describes both standard and state-of-the-art fabrication methods. It reviews current and next generation approaches to assay detection and presents a wide range of applications, from biomolecular interaction discovery and characterization to immune response profiling. The book also addresses several fundamental computational issues, bioinformatics and data analysis.
Book Synopsis Microarrays in Clinical Diagnostics by : Thomas O. Joos
Download or read book Microarrays in Clinical Diagnostics written by Thomas O. Joos and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Book Synopsis Novel Antibody Microarray Technologies for Multiplex Protein Analysis in Complex Samples by : Huiyan Li
Download or read book Novel Antibody Microarray Technologies for Multiplex Protein Analysis in Complex Samples written by Huiyan Li and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "The enzyme-linked immunosorbent assay (ELISA) is the golden standard for the measurement of proteins, but can only detect one protein at each time in the conventional format. Antibody microarrays have been developed to accommodate thousands of micro-spots on a single slide, thus allowing multiple immunoassays to be conducted in parallel. To date antibody microarrays have been used for various applications including the discovery of protein biomarkers for a variety of cancers and other diseases. However, the relatively long assay time from hours to overnight and low multiplexing capability with a maximum of ~50 proteins for sandwich assays are still the main challenges limiting the wide use of antibody microarrays to perform immunoassays. In this dissertation, we have developed novel formats of antibody microarrays to overcome the mass transport and multiplexing limitations, and have demonstrated their use for multiple protein analysis in blood. Specifically, (i) we have developed a beads-in-gel droplet microarray (BiGDM) that encapsulates antibody coated microbeads in 3D hydrogel droplets spotted onto a glass slide. The 3D architecture and the highly porous gels help enhance mass transport of proteins during the assays; (ii) we have established a novel technology named "snap chip" to colocalize cognate capture and detection antibodies in an antibody colocalization microarray (ACM) format by transferring antibodies from microarray-to-microarray in a snap apparatus, thus improving multiplexing capability and simplifying ACM procedure; (iii) we have further improved the multiplexing capability of the snap chip by a double transfer process with improved alignment accuracy, and designed a second version of the portable and convenient-to-use snap apparatus; (iv) we have finally used the snap chip for a serial analysis of multiple proteins in sera during the progression of human breast tumor in a mouse model. The novel antibody microarrays reported in this dissertation have introduced new avenues to overcome mass transport and multiplexing challenges in conventional microarrays, and their use in the detection of multiple proteins in blood indicates that they are promising technologies for the discovery and validation of protein biomarkers for a variety of diseases including notably cancer. " --
Book Synopsis Microarrays by : Hans-Joachim Müller
Download or read book Microarrays written by Hans-Joachim Müller and published by Academic Press. This book was released on 2006 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray technology allows us to answer many questions about gene expression and drug-target screening by employing high-throughput screening. This book dedicates itself to microarrays with clear and understandable explanations and an overview of the presently available hardware, biochips and software. Separate chapters cover the different requirements for DNA and protein chips as well as spotters and scanners.
Download or read book Microarrays written by Jang B. Rampal and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 927 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.
Book Synopsis Protein Microarray for Disease Analysis by : Catherine J. Wu
Download or read book Protein Microarray for Disease Analysis written by Catherine J. Wu and published by Humana Press. This book was released on 2011-08-25 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.
