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Nanolithographic Control Of Double Stranded Dna At The Single Molecule Level
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Book Synopsis Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level by : Teresa Fazio
Download or read book Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level written by Teresa Fazio and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultimately, this results in a 100X improvement in experimental throughput, and a corresponding increase in statistically signicant amounts of data. We also demonstrate site-specific labeling of DNA using DNA analogues, such as peptide nucleic acid (PNA), locked nucleic acid (LNA), and techniques such as nick-translation. Through PNA invasion, labeled DNA was self-assembled in arrays on surfaces and tagged with gold nanoparticles. In this work, DNA formed a template to self-assemble a nanoparticle in between nanoimprinted AuPd dots. Surface-based self-assembly methods offer potential for DNA employment in bottom-up construction of nanoscale arrays. This offers further proof that DNA can be useful in directed self-assembly of nanoscale architectures.
Book Synopsis Studying Self-entangled DNA at the Single Molecule Level by : Christopher Benjamin Renner
Download or read book Studying Self-entangled DNA at the Single Molecule Level written by Christopher Benjamin Renner and published by . This book was released on 2015 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knots seem to be found every time one encounters long, stringy objects. At the macroscopic scale, knots are seen every day in shoelaces, tangled hair, or woven clothing, yet they also present themselves at the microscopic scale in long polymer molecules. Knots can be found often in DNA packaged within the viral capsid, occasionally in proteins, and during the transcription and replication of genomic DNA. Biological knots are similarly thought to change the dynamics of viral ejection, protein digestion, and translocation of biomolecules through nanopores. Despite the prevalence of knots in important biological polymers, to date, the physics of knots is only partially understood. DNA has become a well-accepted model system for investigating the physics of single polymer molecules due to its tremendous biological significance and useful experimental properties. Recent advances in microscopy and nanofabrication have enabled the real-time manipulation and imaging of single DNA molecules, facilitating fundamental studies concerning the physics of individual polymers. Leveraging these experimental techniques, this thesis aims to explore the changes knots can impart on the static and dynamic properties of single DNA molecules. We first demonstrate a mechanism for the previously observed phenomenon of the compression and self-knotting of a single DNA molecule in the presence of an electric field. We then use this mechanism to study the process of stretching complex DNA knots in an extensional field. These knots dramatically alter the way DNA stretches in two ways: an initially arrested state and a subsequently slowed stretching phase. Our work consists of the first experimental support of these phenomena, originally predicted by simulation and theory. We then develop theoretical arguments, shown to agree with simulation results, for the physics that govern the distribution of sizes of knots that stochastically occur on DNA molecules, and more broadly, all semiflexible polymers. We then extend our theory to the case where the entire DNA molecule is confined and elongated within a channel. Here, the complex non-monotonic behavior of the sizes of knots agrees with our modified theory. We finally present the results of dynamical simulations where knots on polymers interact with flows or forces. We first examine the behavior of a knot along a polymer extended by extensional flow. The flow may cause a knot to be swept off a polymer molecule, and the motion of a knot is consistent with a model. Different families of knots display different rates of motion, and we explain this difference with a simple topological mechanism. We then turn to examine the case of knots jamming on a polymer molecule extended with high tensile forces. A simple energy barrier hopping argument qualitatively explains the observed slowdown in dynamics of knots. We use these results to reexamine the problem of DNA knots jamming during nanopore translocation, and our results establish the potential for using knots to slow and control the rate of translocation by a ratcheting mechanism. The impact of this thesis is threefold. First, we have demonstrated a novel experimental platform capable of interrogating DNA knots, likely the most efficient of its kind. Second, we have established a theoretical framework for the size and probability of knotting in single molecules capable of directing experiments where these properties need to be controlled. Finally, we have shown how knotted topologies can be manipulated by external flows or forces, which have applications involving preconditioning molecules to unknotted states or the jamming of knotted molecules in nanopores.
Book Synopsis Spectroscopy and Dynamics of Single Molecules by :
Download or read book Spectroscopy and Dynamics of Single Molecules written by and published by Elsevier. This book was released on 2019-08-14 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. - Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods - Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach - Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction
Book Synopsis Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies by : Amani Hariri
Download or read book Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies written by Amani Hariri and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "By taking it out of its biological role, DNA has served as a robust, reliable and programmable nanofabrication building block for the assembly of a variety of two- and three-dimensional discrete nanometer-sized structures with arbitrary shapes and designs. These molecularly addressable materials could serve as platforms for the site-specific hybridization with nanometer precision. Besides structural complexity, DNA objects evolved to comprise an intrinsic dynamic character allowing them to respond to surrounding stimuli in a predictable manner. Many researchers have previously constructed nanostructures using methods that rely on the spontaneous assembly of DNA in solution, in which case the more complex the assembly design is, the more error-prone the product becomes. This constitutes a major problem for the application of DNA-based structures in dynamic devices such as machines, motors, robots, and computers, where even small errors in assembly can drastically affect performance. Therefore, quantitative tools for analysing structures and dynamics of complex DNA nanostructures are critical. Single molecule fluorescence techniques have been transformative to the field of nanoscience and have played a leading role in providing a mature understanding and an informative feedback on the building blocks and final products of self-assembly. This thesis demonstrates how different single molecule fluorescence methodologies can be utilized to study and advance the structure, dynamics and integrity of DNA-assemblies, specifically those shaped as nanotubes. Conceptually, this research can be divided into four main parts: (i) synthesis optimization, (ii) structural characterization, (iii) monitoring dynamic structures, and (iv) studying structural dynamics. First, a solid-phase synthesis strategy and its visualization through single-molecule spectroscopy was devised to assemble DNA nanotubes in a stepwise fashion, with a full control over their size and sequence pattern. This method paves the way for the production of custom-made DNA nanotubes with fewer structural flaws than the spontaneous-assembly method. Second, single molecule photobleaching and two-color colocalization approaches were uniquely combined to provide a systematic way of assessing the polydispersity, stoichiometry and degree of defectiveness of different DNA nanotubes structures. These approaches will be of significant importance for many research groups synthesizing large supramolecular structures or studying naturally occurring ones, such as protein clusters, amyloids, etc. Third, in situ single molecule immobilization-based fluorescence microscopy was employed to introduce structural changes into DNA nanotubes by dynamically adjusting one or several of the edge lengths between the building blocks using a combination of strand displacement and loops. This is interesting for sensing applications, especially when the analyte produces large scale, detectable structural changes, and also for creating molecular muscles, capable of extension and contraction under external control. Lastly, dynamics and robustness of DNA nanotubes, reconfigured in response to site-specific deletion of DNA strands, were investigated using two color single molecule microscopy. This strategy enables to develop a better understanding of the collective structural changes within DNA structures in response to modifications in their repeat unit. Together, the different methods developed in this thesis underline the importance of single molecule techniques as powerful tools which can advance the field of DNA nanotechnology by enabling the production of well-defined high-quality objects that can meet the designer's compositional and dynamic specifications." --
Book Synopsis 21st Century Nanoscience – A Handbook by : Klaus D. Sattler
Download or read book 21st Century Nanoscience – A Handbook written by Klaus D. Sattler and published by CRC Press. This book was released on 2020-04-08 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 21st Century Nanoscience Handbook will be the most comprehensive, up-to-date large reference work for the field of nanoscience. Handbook of Nanophysics by the same editor published in the fall of 2010 and was embraced as the first comprehensive reference to consider both fundamental and applied aspects of nanophysics. This follow-up project has been conceived as a necessary expansion and full update that considers the significant advances made in the field since 2010. It goes well beyond the physics as warranted by recent developments in the field. This seventh volume in a ten-volume set covers bioinspired systems and methods. Key Features: Provides the most comprehensive, up-to-date large reference work for the field. Chapters written by international experts in the field. Emphasises presentation and real results and applications. This handbook distinguishes itself from other works by its breadth of coverage, readability and timely topics. The intended readership is very broad, from students and instructors to engineers, physicists, chemists, biologists, biomedical researchers, industry professionals, governmental scientists, and others whose work is impacted by nanotechnology. It will be an indispensable resource in academic, government, and industry libraries worldwide. The fields impacted by nanophysics extend from materials science and engineering to biotechnology, biomedical engineering, medicine, electrical engineering, pharmaceutical science, computer technology, aerospace engineering, mechanical engineering, food science, and beyond.
Book Synopsis Structural DNA Nanotechnology by : Nadrian C. Seeman
Download or read book Structural DNA Nanotechnology written by Nadrian C. Seeman and published by Cambridge University Press. This book was released on 2015 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.
Book Synopsis Campanian Foraminifera from the Stanford University Campus, California by : Joseph John Graham
Download or read book Campanian Foraminifera from the Stanford University Campus, California written by Joseph John Graham and published by . This book was released on 1963 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optical Antennas written by Mario Agio and published by Cambridge University Press. This book was released on 2013-01-03 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: This consistent and systematic review of recent advances in optical antenna theory and practice brings together leading experts in the fields of electrical engineering, nano-optics and nano-photonics, physical chemistry and nanofabrication. Fundamental concepts and functionalities relevant to optical antennas are explained, together with key principles for optical antenna modelling, design and characterisation. Recognising the tremendous potential of this technology, practical applications are also outlined. Presenting a clear translation of the concepts of radio antenna design, near-field optics and field-enhanced spectroscopy into optical antennas, this interdisciplinary book is an indispensable resource for researchers and graduate students in engineering, optics and photonics, physics and chemistry.
Book Synopsis Textbook of Pharmaceutical Biotechnology by : Chandrakant Kokate
Download or read book Textbook of Pharmaceutical Biotechnology written by Chandrakant Kokate and published by Elsevier Health Sciences. This book was released on 2012-05-14 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Textbook of Pharmaceutical Biotechnology
Book Synopsis Handbook of Single-Molecule Biophysics by : Peter Hinterdorfer
Download or read book Handbook of Single-Molecule Biophysics written by Peter Hinterdorfer and published by Springer Science & Business Media. This book was released on 2009-12-24 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Book Synopsis Textbook of Pharmaceutical Biotechnology - E-Book by : Chandrakant Kokate
Download or read book Textbook of Pharmaceutical Biotechnology - E-Book written by Chandrakant Kokate and published by Elsevier Health Sciences. This book was released on 2016-04-27 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Textbook of Pharmaceutical Biotechnology - E-Book
Book Synopsis Applications of Microfluidic Systems in Biology and Medicine by : Manabu Tokeshi
Download or read book Applications of Microfluidic Systems in Biology and Medicine written by Manabu Tokeshi and published by Springer. This book was released on 2019-04-25 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on state-of-the-art microfluidic research in medical and biological applications. The top-level researchers in this research field explain carefully and clearly what can be done by using microfluidic devices. Beginners in the field —undergraduates, engineers, biologists, medical researchers—will easily learn to understand microfluidic-based medical and biological applications. Because a wide range of topics is summarized here, it also helps experts to learn more about fields outside their own specialties. The book covers many interesting subjects, including cell separation, protein crystallization, single-cell analysis, cell diagnosis, point-of-care testing, immunoassay, embyos/worms on a chip and organ-on-a-chip. Readers will be convinced that microfluidic devices have great potential for medical and biological applications.
Author :Management Association, Information Resources Publisher :IGI Global ISBN 13 :1466651261 Total Pages :1654 pages Book Rating :4.4/5 (666 download)
Book Synopsis Nanotechnology: Concepts, Methodologies, Tools, and Applications by : Management Association, Information Resources
Download or read book Nanotechnology: Concepts, Methodologies, Tools, and Applications written by Management Association, Information Resources and published by IGI Global. This book was released on 2014-02-28 with total page 1654 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past few decades, devices and technologies have been significantly miniaturized from one generation to the next, providing far more potential in a much smaller package. The smallest of these recently developed tools are miniscule enough to be invisible to the naked eye. Nanotechnology: Concepts, Methodologies, Tools, and Applications describes some of the latest advances in microscopic technologies in fields as diverse as biochemistry, materials science, medicine, and electronics. Through its investigation of theories, applications, and new developments in the nanotechnology field, this impressive reference source will serve as a valuable tool for researchers, engineers, academics, and students alike.
Book Synopsis Bionanotechnology by : David S. Goodsell
Download or read book Bionanotechnology written by David S. Goodsell and published by John Wiley & Sons. This book was released on 2004-04-16 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discussions of the basic structural, nanotechnology, and system engineering principles, as well as an introductory overview of essential concepts and methods in biotechnology, will be included. Text is presented side-by-side with extensive use of high-quality illustrations prepared using cutting edge computer graphics techniques. Includes numerous examples, such applications in genetic engineering. Represents the only available introduction and overview of this interdisciplinary field, merging the physical and biological sciences. Concludes with the authors' expert assessment of the future promise of nanotechnology, from molecular "tinkertoys" to nanomedicine. David Goodsell is author of two trade books, Machinery of Life and Our Molecular Nature, and Arthur Olson is the world's leader in molecular graphics and nano-scale representation.
Download or read book Technology Review written by and published by . This book was released on 2002 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Physics of Bio-Molecules and Cells by : Henrik Flyvbjerg
Download or read book Physics of Bio-Molecules and Cells written by Henrik Flyvbjerg and published by Springer Science & Business Media. This book was released on 2003-07-01 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aimed at those working to enter this rapidly developing field, this volume on biological physics is written in a pedagogical style by leading scientists giving explanations that take their starting point where any physicist can follow and end at the frontier of research in biological physics. These lectures describe the state-of-the-art physics of biomolecules and cells. In biological systems ranging from single biomolecules to entire cells and larger biological systems, it focuses on aspects that require concepts and methods from physics for their analysis and understanding, such as the mechanics of motor proteins; how the genetic code is physically read and managed; the machinery of protein--DNA interactions; force spectroscopy of biomolecules' velopes, cytoskeletons, and cytoplasms; polymerization forces; listeria propulsion; cell motility; lab-on-a-chip nanotechnology for single-molecule analysis of biomolecules; bioinformatics; and coding and computational strategies of the brain.
Download or read book Nanoparticles written by Günter Schmid and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introduction to the science of nanoparticles, from fundamental principles to their use in novel applications. As a basis for understanding nanoparticle behavior, the book first outlines the principles of quantum size behavior, nanoparticles architecture, formation of semiconductor and metal nanoparticles. It then goes on to describe the chemical syntheses of nanoparticles with defined characteristics, their structural, electrical and magnetic properties, as well as current methods to monitor these properties. Among others, the following nanoparticle-based applications are discussed: Single-electron devices Ultra dense recording media Bioelectronic devices and sensors Labeling of proteins, nucleic acids and other biomaterials. With its clear structure and comprehensive coverage, backed by numerous examples from the recent literature, this is a prime reference for chemists and materials scientists working with and developing nanoparticle systems.
