Molecular Histochemical Techniques

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Molecular Histochemical Techniques

Author : Takehiko Koji
Publisher : Springer Science & Business Media
ISBN 13 : 4431679154
Total Pages : 270 pages
Book Rating : 4.4/5 (316 download)

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Book Synopsis Molecular Histochemical Techniques by : Takehiko Koji

Download or read book Molecular Histochemical Techniques written by Takehiko Koji and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. It describes in situ nick translation and TUNEL in detail, along with radioactive and non-radioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. The methodology is set out in easy-to-follow language by leading scientists working in the field today. Both a rich source of the latest information and a practical lab manual.

Histochemistry of Single Molecules

Author : Carlo Pellicciari
Publisher : Humana
ISBN 13 : 9781071626771
Total Pages : 0 pages
Book Rating : 4.6/5 (267 download)

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Book Synopsis Histochemistry of Single Molecules by : Carlo Pellicciari

Download or read book Histochemistry of Single Molecules written by Carlo Pellicciari and published by Humana. This book was released on 2023-10-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Histological & Histochemical Methods

Author : John Alan Kiernan
Publisher : Pergamon
ISBN 13 :
Total Pages : 454 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Histological & Histochemical Methods by : John Alan Kiernan

Download or read book Histological & Histochemical Methods written by John Alan Kiernan and published by Pergamon. This book was released on 1990 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

Histochemistry of Single Molecules

Author : Carlo Pellicciari
Publisher : Springer Nature
ISBN 13 : 1071626752
Total Pages : 351 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Histochemistry of Single Molecules by : Carlo Pellicciari

Download or read book Histochemistry of Single Molecules written by Carlo Pellicciari and published by Springer Nature. This book was released on 2022-09-24 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Molecular Histochemical Techniques

Author : Takehiko Koji
Publisher :
ISBN 13 : 9784431679165
Total Pages : 276 pages
Book Rating : 4.6/5 (791 download)

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Book Synopsis Molecular Histochemical Techniques by : Takehiko Koji

Download or read book Molecular Histochemical Techniques written by Takehiko Koji and published by . This book was released on 2000-04-17 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Histochemical and Cytochemical Methods of Visualization

Author : Jean-Marie Exbrayat
Publisher : CRC Press
ISBN 13 : 1439822255
Total Pages : 367 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Histochemical and Cytochemical Methods of Visualization by : Jean-Marie Exbrayat

Download or read book Histochemical and Cytochemical Methods of Visualization written by Jean-Marie Exbrayat and published by CRC Press. This book was released on 2013-06-05 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio

Bancroft's Theory and Practice of Histological Techniques E-Book

Author : Kim S Suvarna
Publisher : Elsevier Health Sciences
ISBN 13 : 0702050326
Total Pages : 655 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Bancroft's Theory and Practice of Histological Techniques E-Book by : Kim S Suvarna

Download or read book Bancroft's Theory and Practice of Histological Techniques E-Book written by Kim S Suvarna and published by Elsevier Health Sciences. This book was released on 2012-10-01 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.

Histological and Histochemical Methods, fifth edition

Author : John Kiernan
Publisher : Scion Publishing Ltd
ISBN 13 : 1907904778
Total Pages : 589 pages
Book Rating : 4.9/5 (79 download)

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Book Synopsis Histological and Histochemical Methods, fifth edition by : John Kiernan

Download or read book Histological and Histochemical Methods, fifth edition written by John Kiernan and published by Scion Publishing Ltd. This book was released on 2015-06-08 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth edition of Histological and Histochemical Methods continues to provide a clear and consistent introduction to the techniques, description and analysis of the chemical and physical principles of fixation, tissue processing, staining, enzyme location, immunohistochemistry and other key procedures. The overall structure of the book remains unchanged, but the content has been heavily revised to update the techniques used in line with recent technological advances. Additionally, there are new sections on: Artefacts and troubleshooting Methods for microorganisms and fungi in sections Methods for various pigments and mineral deposits in tissues Methods for skeletal elements (bone, cartilage) in whole-mounts Histological and Histochemical Methods 5e is essential reading for students, lecturers, researchers and professionals using histological and histochemical techniques. From reviews: "Histological and Histochemical Methods is a tour de force wholly suited to the modern age of histology and Professor Kiernan has triumphed again. To cover so much ground clearly and concisely while including the justification of the underlying chemistry makes this book unique. There should not be a histology laboratory or an undergraduate library that does not own a copy." Biotechnic & Histochemistry 2016, 91(2): 145. "This book should be present on the bookshelves of every research or analysis laboratory where histology and histochemistry are routinely used, as an essential reference source of basic and practical information for scientists and technicians." European Journal of Histochemistry, 2016, vol. 60.

Histochemical and Immunohistochemical Techniques

Author : Peter H. Bach
Publisher : Springer Science & Business Media
ISBN 13 : 9401130949
Total Pages : 292 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Histochemical and Immunohistochemical Techniques by : Peter H. Bach

Download or read book Histochemical and Immunohistochemical Techniques written by Peter H. Bach and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.

Histochemistry

Author : Jinsong Zhou
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110531399
Total Pages : 242 pages
Book Rating : 4.1/5 (15 download)

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Book Synopsis Histochemistry by : Jinsong Zhou

Download or read book Histochemistry written by Jinsong Zhou and published by Walter de Gruyter GmbH & Co KG. This book was released on 2017-08-21 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.

Handbook of Histopathological and Histochemical Techniques

Author : C. F. A. Culling
Publisher : Butterworth-Heinemann
ISBN 13 : 1483164799
Total Pages : 727 pages
Book Rating : 4.4/5 (831 download)

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Book Synopsis Handbook of Histopathological and Histochemical Techniques by : C. F. A. Culling

Download or read book Handbook of Histopathological and Histochemical Techniques written by C. F. A. Culling and published by Butterworth-Heinemann. This book was released on 2013-10-22 with total page 727 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques. This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. The remaining chapters focus on the preparation, mounting, and presentation of museum specimens. The book concludes with an analysis of the microscope. This monograph will be invaluable to all those with an interest in histology and histochemistry, including undergraduate students in the biological and health sciences, pathologists in both the hospital setting and experimental research, and technologists and workers in various research fields.

Histochemistry in Focus

Author : K. Shyamsundari
Publisher : MJP Publisher
ISBN 13 :
Total Pages : 886 pages
Book Rating : 4./5 ( download)

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Book Synopsis Histochemistry in Focus by : K. Shyamsundari

Download or read book Histochemistry in Focus written by K. Shyamsundari and published by MJP Publisher. This book was released on 2019-06-07 with total page 886 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fixatives and Methods of Fixation, Tissue Processing, Theory of Staining, Decalcification, Preparation of Stains, Mountants, Frozen Methods, Carbohydrates, Protein, Amyloids, Nucleic Acids, Lipids, Pigments, Minerals, Microorganisms in Sections, Enzymes, Connective Tissue, Neurological Studies, Endocrine Glands, Microwave Histology, Ultrahistochemistry, Techniques in Cell Biology, Methods for Special Organs, Invertebrate Staining Methods, Mast Cells, Immunocytochemistry.

组织学技术

Author :
Publisher :
ISBN 13 : 9787811165777
Total Pages : 725 pages
Book Rating : 4.1/5 (657 download)

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Book Synopsis 组织学技术 by :

Download or read book 组织学技术 written by and published by . This book was released on 2008 with total page 725 pages. Available in PDF, EPUB and Kindle. Book excerpt: 本书内容全面,涵盖了组织学技术的方方面面,既包括传统的常用技术,又介绍了近年来新发展的,有较高实用价值的新技术。各章节分别论述了光镜、组织固定、样本处理、冰冻切片、石蜡切片、酶组织学、免疫组化、免疫荧光、组织芯片、原位杂交、荧光原位杂交、激光显微解剖、电镜等技术;对于结缔组织、糖类、脂类、蛋白质、核酸、色素及矿物质、淀粉、神经内分泌颗粒、微体、骨等相关的组绢学技术也予专门章节讲述;另外还涉及了显微镜样品定量、实验室安全及管理,以及人体工程学等相关内容。

Histochemical Techniques

Author : J. D. Bancroft
Publisher : Butterworth-Heinemann
ISBN 13 : 1483164713
Total Pages : 363 pages
Book Rating : 4.4/5 (831 download)

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Book Synopsis Histochemical Techniques by : J. D. Bancroft

Download or read book Histochemical Techniques written by J. D. Bancroft and published by Butterworth-Heinemann. This book was released on 2013-10-22 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.

Enzyme Histochemistry

Author : Z. Lojda
Publisher : Springer Science & Business Media
ISBN 13 : 3642672345
Total Pages : 350 pages
Book Rating : 4.6/5 (426 download)

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Book Synopsis Enzyme Histochemistry by : Z. Lojda

Download or read book Enzyme Histochemistry written by Z. Lojda and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Histology Protocols

Author : Tim D. Hewitson
Publisher : Humana
ISBN 13 : 9781493956944
Total Pages : 229 pages
Book Rating : 4.9/5 (569 download)

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Book Synopsis Histology Protocols by : Tim D. Hewitson

Download or read book Histology Protocols written by Tim D. Hewitson and published by Humana. This book was released on 2016-08-23 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols.

Morphology Methods

Author : Ricardo V. Lloyd
Publisher : Springer Science & Business Media
ISBN 13 : 1592591906
Total Pages : 435 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Morphology Methods by : Ricardo V. Lloyd

Download or read book Morphology Methods written by Ricardo V. Lloyd and published by Springer Science & Business Media. This book was released on 2001-06-15 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.