Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications

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Publisher : Frontiers Media SA
ISBN 13 : 288971523X
Total Pages : 221 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications by : Vicente Micó

Download or read book Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and published by Frontiers Media SA. This book was released on 2021-10-22 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microscopy, Optical Spectroscopy, and Macroscopic Techniques

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Publisher : Springer Science & Business Media
ISBN 13 : 159259509X
Total Pages : 260 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Microscopy, Optical Spectroscopy, and Macroscopic Techniques by : Christopher Jones

Download or read book Microscopy, Optical Spectroscopy, and Macroscopic Techniques written by Christopher Jones and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.

Optical Spectroscopic and Microscopic Techniques

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Publisher :
ISBN 13 : 9789811645518
Total Pages : 0 pages
Book Rating : 4.6/5 (455 download)

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Book Synopsis Optical Spectroscopic and Microscopic Techniques by : Harekrushna Sahoo

Download or read book Optical Spectroscopic and Microscopic Techniques written by Harekrushna Sahoo and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.

Optical Techniques in Biological Research

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Publisher : Elsevier
ISBN 13 : 0323150217
Total Pages : 442 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Optical Techniques in Biological Research by : Denis Rousseau

Download or read book Optical Techniques in Biological Research written by Denis Rousseau and published by Elsevier. This book was released on 2012-12-02 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.

Atomic Force Microscopy in Liquid

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Publisher : John Wiley & Sons
ISBN 13 : 3527327584
Total Pages : 385 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró

Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-05-14 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Optical Techniques in Biological Research

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Publisher :
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Optical Techniques in Biological Research by : Denis L. Rousseau

Download or read book Optical Techniques in Biological Research written by Denis L. Rousseau and published by . This book was released on 1984 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Light scattering / Bennie R. Ware -- Raman scattering / Denis L. Rousseau and Mark R. Ondrias -- Fourier transform infrared spectroscopy / James O. Alben and Frank G. Fiamingo -- Nanosecond fluorescence spectroscopy / Juan Yguerabide and Evangelina E. Yguerabide -- Circular dichroism / Marcos F. Maestre -- Electron microscopy / S.W. Hui.

Biochemical Applications of Nonlinear Optical Spectroscopy

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Publisher : CRC Press
ISBN 13 : 1420068601
Total Pages : 290 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Biochemical Applications of Nonlinear Optical Spectroscopy by : Vladislav V. Yakovlev

Download or read book Biochemical Applications of Nonlinear Optical Spectroscopy written by Vladislav V. Yakovlev and published by CRC Press. This book was released on 2018-10-08 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: For a host of reasons, nonlinear optical spectroscopy is a valuable tool for biochemical applications where minimally invasive diagnostics is desired. Biochemical Applications of Nonlinear Optical Spectroscopy presents the latest technological advances and offers a perspective on future directions in this important field. Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and non-linear Raman microspectroscopy. The text explores the promise of beam shaping and the use of broadband laser pulse generated through continuum generation and an optical pulse shaper. Lastly, the book discusses the effects of spatial beam shaping on the generated nonlinear Raman signals in a tightly focused geometry and provides insight into the extension of nonlinear optical spectroscopy to the nanoscale through the use of plasmonic tip-enhanced arrangement. With novel experimental approaches to this technology expanding day-by-day, the book’s balanced coverage from a wide range of international contributors not only elucidates important achievements, but also outlines future directions in this dynamic and promising field.

Optical Techniques

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Publisher : Academic Press
ISBN 13 : 1483282074
Total Pages : 578 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Optical Techniques by : Gerald Oster

Download or read book Optical Techniques written by Gerald Oster and published by Academic Press. This book was released on 2013-09-03 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Techniques in Biological Research, Volume I: Optical Techniques focuses on improvements in physical techniques used in biological research on cells and tissues. The selection first discusses photochemistry and luminescence and light scattering, including applications of luminescence, theory of light scattering and its applications, and light scattering apparatus. The text then examines absorption spectroscopy, ultraviolet absorption spectrophotometry, and infrared spectrophotometry. Discussions focus on factors involved in data gathering, empirical correlation between molecular structure and absorption spectra, buffers for ultraviolet absorption spectrophotometry, instrumentation and techniques, and interpretation of data. The text ponders on the light microscope and phase and interference microscopy, as well as the optical and mechanical systems of microscopes; wave nature of light and its consequences; purposes of phase and interference microscopy; and principles of phase microscopy. The publication also reviews birefringence and dichroism and electron microscopy. The selection is highly recommended for students and readers interested in the physical techniques used in biological research.

Handbook of Biomedical Nonlinear Optical Microscopy

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Publisher : Oxford University Press on Demand
ISBN 13 : 0195162609
Total Pages : 895 pages
Book Rating : 4.1/5 (951 download)

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Book Synopsis Handbook of Biomedical Nonlinear Optical Microscopy by : Barry R. Masters

Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press on Demand. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ideal for cell biologists, life scientists, biomedical engineers, and clinicians, this handbook provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy.

Optical Spectroscopy and Computational Methods in Biology and Medicine

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Publisher : Springer Science & Business Media
ISBN 13 : 9400778325
Total Pages : 540 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Optical Spectroscopy and Computational Methods in Biology and Medicine by : Malgorzata Baranska

Download or read book Optical Spectroscopy and Computational Methods in Biology and Medicine written by Malgorzata Baranska and published by Springer Science & Business Media. This book was released on 2013-12-05 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: This multi-author contributed volume gives a comprehensive overview of recent progress in various vibrational spectroscopic techniques and chemometric methods and their applications in chemistry, biology and medicine. In order to meet the needs of readers, the book focuses on recent advances in technical development and potential exploitations of the theory, as well as the new applications of vibrational methods to problems of recent general interest that were difficult or even impossible to achieve in the not so distant past. Integrating vibrational spectroscopy and computational approaches serves as a handbook for people performing vibrational spectroscopy followed by chemometric analysis hence both experimental methods as well as procedures of recommended analysis are described. This volume is written for individuals who develop new methodologies and extend these applications to new realms of chemical and medicinal interest.

Lasers and Current Optical Techniques in Biology

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Publisher : Royal Society of Chemistry
ISBN 13 : 9780854043217
Total Pages : 688 pages
Book Rating : 4.0/5 (432 download)

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Book Synopsis Lasers and Current Optical Techniques in Biology by : Giuseppe Palumbo

Download or read book Lasers and Current Optical Techniques in Biology written by Giuseppe Palumbo and published by Royal Society of Chemistry. This book was released on 2004 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: The wide range of topics covered make this book of interest to a diverse range of scientific communities.

Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation

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Publisher : Springer
ISBN 13 : 9048199778
Total Pages : 434 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation by : Baldassare Di Bartolo

Download or read book Biophotonics: Spectroscopy, Imaging, Sensing, and Manipulation written by Baldassare Di Bartolo and published by Springer. This book was released on 2010-12-14 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes an impressive array of the current photonic-related technologies being used in the investigation of biological systems. The topics include various types of microscopy (fluorescence correlation microscopy, two-photon microscopy), sensitive detection of biological molecules, nano-surgery techniques, fluorescence resonance energy transfer, nano-plasmonics, terahertz spectroscopy, and photosynthetic energy conversion. The emphasis is on the physical principles behind each technique, and on examining the advantages and limitations of each.The book begins with an overview by Paras Prasad, a leader in the field of biophotonics, of several important optical techniques currently used for studying biological systems. In the subsequent chapters these techniques are discussed in depth, providing the reader with a detailed understanding of the basic physical principles at work. An excellent treatment of terahertz spectroscopy demonstrates how photonics is being extended beyond the visible region. Recent results in the use of femtosecond lasers as a tool to porate cell walls demonstrate that the manipulation of light can be used as a tool for the study and the treatment of biological systems. The field of Bio-photonics is broad and still growing, so cannot be covered comprehensively in one volume. But here the reader will find an introduction to some of the major tools used for studying biological systems, and at the same time a detailed, first-principles treatment of the physics behind these tools.

Microscopic and Spectroscopic Imaging of the Chemical State

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Publisher : CRC Press
ISBN 13 : 9780824791049
Total Pages : 508 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis Microscopic and Spectroscopic Imaging of the Chemical State by : Michael D. Morris

Download or read book Microscopic and Spectroscopic Imaging of the Chemical State written by Michael D. Morris and published by CRC Press. This book was released on 1993-07-26 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents chemical state imaging methods useful on distance scales ranging from individual atoms to millimeters. This work is intended for chemists familiar with modern spectroscopies, but includes tutorial material on basic imaging processes for those with little background in the field.

Super Resolution Optical Imaging and Microscopy

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Publisher : John Wiley & Sons
ISBN 13 : 3527835520
Total Pages : 261 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Super Resolution Optical Imaging and Microscopy by : Junle Qu

Download or read book Super Resolution Optical Imaging and Microscopy written by Junle Qu and published by John Wiley & Sons. This book was released on 2023-12-15 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.

Biophotonics

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Publisher : World Scientific
ISBN 13 : 9813235705
Total Pages : 292 pages
Book Rating : 4.8/5 (132 download)

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Book Synopsis Biophotonics by : Yeh Yin

Download or read book Biophotonics written by Yeh Yin and published by World Scientific. This book was released on 2018-06-28 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This lecture volume aims to give students and researchers in this rapidly expanding field of biophotonics an interdisciplinary perspective. Among the primary topics are ultrahigh resolution microscopy, particle tracking, photon correlation spectroscopy, and nonlinear optical methods as used in biological and biomedical research, with a focus on current applications in biophysics and biomedicine.

Far-Field Optical Nanoscopy

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Publisher : Springer
ISBN 13 : 3662455471
Total Pages : 335 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Far-Field Optical Nanoscopy by : Philip Tinnefeld

Download or read book Far-Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Modern Optical Spectroscopy

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Publisher : Springer Nature
ISBN 13 : 3031172221
Total Pages : 653 pages
Book Rating : 4.0/5 (311 download)

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Book Synopsis Modern Optical Spectroscopy by : William W. Parson

Download or read book Modern Optical Spectroscopy written by William W. Parson and published by Springer Nature. This book was released on 2023-02-28 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 3rd edition of this textbook offers clear explanations of optical spectroscopic phenomena and shows how spectroscopic techniques are used in modern chemistry, biochemistry and biophysics. Topics included are: electronic and vibrational absorption fluorescence symmetry operations and normal-mode calculations electron transfer from excited molecules energy transfer exciton interactions electronic and vibrational circular dichroism coherence and dephasing ultrafast pump-probe and photon-echo spectroscopy single-molecule and fluorescence-correlation spectroscopy Raman scattering multiphoton absorption quantum optics and non-linear optics entropy changes during photoexcitation electronic and vibrational Stark effects studies of fast processes in single molecules two-dimensional electronic and vibrational spectroscopy This revised and updated edition provides expanded discussions of laser spectroscopy, crystal symmetry, birefringence, non-linear optics, solar cells and light-emitting diodes. The explanations are sufficiently thorough and detailed to be useful for researchers, graduate students and advanced undergraduates in chemistry, biochemistry and biophysics. They are based on time-dependent quantum mechanics, but are developed from first principles so that they can be understood by readers with little prior training in the field. Additional topics and highlights are presented in special boxes in the text. The book is richly illustrated with color figures throughout. Each chapter ends with a section of questions for self-examination.