Methods In Plant Tissue Culture

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Plant Tissue Culture

Author : Robert H. Smith
Publisher : Academic Press
ISBN 13 : 0323160476
Total Pages : 190 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Plant Tissue Culture by : Robert H. Smith

Download or read book Plant Tissue Culture written by Robert H. Smith and published by Academic Press. This book was released on 2012-12-02 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Author : Saurabh Bhatia
Publisher : Academic Press
ISBN 13 : 0128024984
Total Pages : 452 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Modern Applications of Plant Biotechnology in Pharmaceutical Sciences by : Saurabh Bhatia

Download or read book Modern Applications of Plant Biotechnology in Pharmaceutical Sciences written by Saurabh Bhatia and published by Academic Press. This book was released on 2015-07-22 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions

Plant Tissue Culture

Author : B. N. Sathyanarayana
Publisher : I. K. International Pvt Ltd
ISBN 13 : 8189866117
Total Pages : 299 pages
Book Rating : 4.1/5 (898 download)

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Book Synopsis Plant Tissue Culture by : B. N. Sathyanarayana

Download or read book Plant Tissue Culture written by B. N. Sathyanarayana and published by I. K. International Pvt Ltd. This book was released on 2007 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Plant Tissue Culture

Author : Roberta H. Smith
Publisher : Academic Press
ISBN 13 : 0124159206
Total Pages : 206 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Plant Tissue Culture by : Roberta H. Smith

Download or read book Plant Tissue Culture written by Roberta H. Smith and published by Academic Press. This book was released on 2013 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Plant Cell Culture Protocols

Author : Víctor M. Loyola-Vargas
Publisher : Springer Science & Business Media
ISBN 13 : 1592599591
Total Pages : 389 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas

Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Plant Cell Culture

Author : Michael R. Davey
Publisher : John Wiley & Sons
ISBN 13 : 9780470686515
Total Pages : 358 pages
Book Rating : 4.6/5 (865 download)

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Book Synopsis Plant Cell Culture by : Michael R. Davey

Download or read book Plant Cell Culture written by Michael R. Davey and published by John Wiley & Sons. This book was released on 2010-05-20 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Plant Cell Culture Protocols

Author : Víctor M. Loyola-Vargas
Publisher : Humana Press
ISBN 13 : 9781617798177
Total Pages : 430 pages
Book Rating : 4.7/5 (981 download)

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Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas

Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Humana Press. This book was released on 2012-05-10 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Plant Cell Culture Protocols

Author : Robert D. Hall
Publisher : Springer Science & Business Media
ISBN 13 : 1592595839
Total Pages : 422 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Cell Culture Protocols by : Robert D. Hall

Download or read book Plant Cell Culture Protocols written by Robert D. Hall and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region

Author : Duong Tan Nhut
Publisher : Springer Nature
ISBN 13 : 9811664986
Total Pages : 400 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region by : Duong Tan Nhut

Download or read book Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region written by Duong Tan Nhut and published by Springer Nature. This book was released on 2022-04-05 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

Experiments in Plant Tissue Culture

Author : John H. Dodds
Publisher : International Potato Center
ISBN 13 : 9780521315166
Total Pages : 260 pages
Book Rating : 4.3/5 (151 download)

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Book Synopsis Experiments in Plant Tissue Culture by : John H. Dodds

Download or read book Experiments in Plant Tissue Culture written by John H. Dodds and published by International Potato Center. This book was released on 1985-10-31 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Plant Tissue Culture Concepts and Laboratory Exercises

Author : Robert N. Trigiano
Publisher : Routledge
ISBN 13 : 1351424033
Total Pages : 470 pages
Book Rating : 4.3/5 (514 download)

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Book Synopsis Plant Tissue Culture Concepts and Laboratory Exercises by : Robert N. Trigiano

Download or read book Plant Tissue Culture Concepts and Laboratory Exercises written by Robert N. Trigiano and published by Routledge. This book was released on 2018-04-27 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Plant Tissue Culture

Author : Sunghun Park
Publisher : Academic Press
ISBN 13 : 0323851371
Total Pages : 254 pages
Book Rating : 4.3/5 (238 download)

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Book Synopsis Plant Tissue Culture by : Sunghun Park

Download or read book Plant Tissue Culture written by Sunghun Park and published by Academic Press. This book was released on 2021-02-17 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012

Plant Cell and Tissue Culture

Author : Indra K. Vasil
Publisher : Springer Science & Business Media
ISBN 13 : 9401726817
Total Pages : 593 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Plant Cell and Tissue Culture by : Indra K. Vasil

Download or read book Plant Cell and Tissue Culture written by Indra K. Vasil and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Plant Tissue Culture

Author : M.P. Singh
Publisher : APH Publishing
ISBN 13 : 9788131304396
Total Pages : 294 pages
Book Rating : 4.3/5 (43 download)

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Book Synopsis Plant Tissue Culture by : M.P. Singh

Download or read book Plant Tissue Culture written by M.P. Singh and published by APH Publishing. This book was released on 2009 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Methods in Plant Tissue Culture

Author : U. Kumar
Publisher :
ISBN 13 : 9788177540772
Total Pages : 460 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Methods in Plant Tissue Culture by : U. Kumar

Download or read book Methods in Plant Tissue Culture written by U. Kumar and published by . This book was released on 2008-07-01 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. Plant Tissue Culture: An Overview, 2. History of Plant Tissue and Cell Culture, 3. Three General Methodology of Plant Tissue Culture, 4. Four Culture Media Ingredients, Preparation and Related Problems, 5. Methods of Sterilization and Disinfestation, 6. Aseptic Techniques and Preparation of Explants, 7. Plant Tissue Culture Some Related Aspects, 8. Histological and Photographic Techniques for Plant Tissue Culture, 9. Protoplast Technology, 10 Ten Micropropagation in Plants, 11. Micropropagation: Commercial Laboratory Production, 12. Twelve Somatic Embryogenesis Principles, Concepts and Application, 13. Somaclonal and Gametoclonal Variant Selection, 14. Synseeds or Synthetic Seeds, 15. Plant Tissue Culture and Germplasm Conservation, 16. Plant Tissue Culture and Secondary Metabolite Production, 17. Indexing for Plant Pathogens, 18. Techniques for Crop Improvement.

Plant Cell, Tissue and Organ Culture

Author : Oluf Gamborg
Publisher : Springer Science & Business Media
ISBN 13 : 3642790488
Total Pages : 367 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Cell, Tissue and Organ Culture by : Oluf Gamborg

Download or read book Plant Cell, Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Plant Cell Culture

Author : Julian Coleman
Publisher : Taylor & Francis
ISBN 13 : 1000144712
Total Pages : 212 pages
Book Rating : 4.0/5 (1 download)

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Book Synopsis Plant Cell Culture by : Julian Coleman

Download or read book Plant Cell Culture written by Julian Coleman and published by Taylor & Francis. This book was released on 2020-07-26 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.