Plant Cell Culture Protocols

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Plant Cell Culture Protocols

Author : Víctor M. Loyola-Vargas
Publisher : Springer Science & Business Media
ISBN 13 : 1592599591
Total Pages : 389 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas

Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Plant Cell Culture Protocols

Author : Víctor M. Loyola-Vargas
Publisher : Humana Press
ISBN 13 : 9781617798177
Total Pages : 430 pages
Book Rating : 4.7/5 (981 download)

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Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas

Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Humana Press. This book was released on 2012-05-10 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Plant Cell Culture

Author : Michael R. Davey
Publisher : John Wiley & Sons
ISBN 13 : 9780470686515
Total Pages : 358 pages
Book Rating : 4.6/5 (865 download)

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Book Synopsis Plant Cell Culture by : Michael R. Davey

Download or read book Plant Cell Culture written by Michael R. Davey and published by John Wiley & Sons. This book was released on 2010-05-20 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Plant Tissue Culture

Author : B. N. Sathyanarayana
Publisher : I. K. International Pvt Ltd
ISBN 13 : 8189866117
Total Pages : 299 pages
Book Rating : 4.1/5 (898 download)

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Book Synopsis Plant Tissue Culture by : B. N. Sathyanarayana

Download or read book Plant Tissue Culture written by B. N. Sathyanarayana and published by I. K. International Pvt Ltd. This book was released on 2007 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Plant Cell Culture

Author : R. A. Dixon
Publisher : IRL Press
ISBN 13 : 9780199634026
Total Pages : 254 pages
Book Rating : 4.6/5 (34 download)

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Book Synopsis Plant Cell Culture by : R. A. Dixon

Download or read book Plant Cell Culture written by R. A. Dixon and published by IRL Press. This book was released on 1994 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.

Plant Cell Culture

Author : Hamish A. Collin
Publisher : Taylor & Francis
ISBN 13 :
Total Pages : 172 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Plant Cell Culture by : Hamish A. Collin

Download or read book Plant Cell Culture written by Hamish A. Collin and published by Taylor & Francis. This book was released on 1998 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the information necessary to set up and run a tissue culture facility is provided in this introductory book.; ; Includes an overview of all the basic tissue culture techniques and describes in detail both the theoretical background and the practical a

Plant Cell, Tissue and Organ Culture

Author : Oluf Gamborg
Publisher : Springer Science & Business Media
ISBN 13 : 3642790488
Total Pages : 367 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Cell, Tissue and Organ Culture by : Oluf Gamborg

Download or read book Plant Cell, Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Plant Cell Culture Protocols

Author : Loyala
Publisher :
ISBN 13 : 9788181288509
Total Pages : 416 pages
Book Rating : 4.2/5 (885 download)

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Book Synopsis Plant Cell Culture Protocols by : Loyala

Download or read book Plant Cell Culture Protocols written by Loyala and published by . This book was released on 2007-12-01 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant Tissue Culture Manual

Author : K. Lindsey
Publisher : Springer Science & Business Media
ISBN 13 : 9780792311157
Total Pages : 108 pages
Book Rating : 4.3/5 (111 download)

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Book Synopsis Plant Tissue Culture Manual by : K. Lindsey

Download or read book Plant Tissue Culture Manual written by K. Lindsey and published by Springer Science & Business Media. This book was released on 1991 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic techniques - cells tissue culture of model species. Tissue culture & transformation of crop species. Propagation & conservation of germplasm. Direct gene transfer & protoplast fusion. Reproductive tissues. Mutant selection.

Plant Tissue Culture

Author : Robert H. Smith
Publisher : Academic Press
ISBN 13 : 0323160476
Total Pages : 190 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Plant Tissue Culture by : Robert H. Smith

Download or read book Plant Tissue Culture written by Robert H. Smith and published by Academic Press. This book was released on 2012-12-02 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Plant Cell and Tissue Culture

Author : Indra K. Vasil
Publisher : Springer Science & Business Media
ISBN 13 : 9401726817
Total Pages : 593 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Plant Cell and Tissue Culture by : Indra K. Vasil

Download or read book Plant Cell and Tissue Culture written by Indra K. Vasil and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Experiments in Plant Tissue Culture

Author : John H. Dodds
Publisher : International Potato Center
ISBN 13 : 9780521315166
Total Pages : 260 pages
Book Rating : 4.3/5 (151 download)

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Book Synopsis Experiments in Plant Tissue Culture by : John H. Dodds

Download or read book Experiments in Plant Tissue Culture written by John H. Dodds and published by International Potato Center. This book was released on 1985-10-31 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Plant Tissue Culture: An Introductory Text

Author : Sant Saran Bhojwani
Publisher : Springer Science & Business Media
ISBN 13 : 8132210263
Total Pages : 318 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Plant Tissue Culture: An Introductory Text by : Sant Saran Bhojwani

Download or read book Plant Tissue Culture: An Introductory Text written by Sant Saran Bhojwani and published by Springer Science & Business Media. This book was released on 2013-03-20 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

Plant Cell Culture

Author : David E. Evans
Publisher : Garland Science
ISBN 13 : 9781859963203
Total Pages : 212 pages
Book Rating : 4.9/5 (632 download)

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Book Synopsis Plant Cell Culture by : David E. Evans

Download or read book Plant Cell Culture written by David E. Evans and published by Garland Science. This book was released on 2003 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.

Basic Cell Culture Protocols

Author : Jeffrey W. Pollard
Publisher : Springer Science & Business Media
ISBN 13 : 0896034410
Total Pages : 477 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard

Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Tissue Culture

Author : Paul F. Jr. Kruse
Publisher : Elsevier
ISBN 13 : 0323142079
Total Pages : 897 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Tissue Culture by : Paul F. Jr. Kruse

Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 897 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Protocols for Neural Cell Culture

Author : Sergey Fedoroff
Publisher : Springer Science & Business Media
ISBN 13 : 1592592074
Total Pages : 366 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Protocols for Neural Cell Culture by : Sergey Fedoroff

Download or read book Protocols for Neural Cell Culture written by Sergey Fedoroff and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.