Low Temperature Biological Microscopy And Analysis

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Low-Temperature Microscopy and Analysis

Author : Patrick Echlin
Publisher : Springer Science & Business Media
ISBN 13 : 1489923020
Total Pages : 553 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Low-Temperature Microscopy and Analysis by : Patrick Echlin

Download or read book Low-Temperature Microscopy and Analysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.

Low Temperature Biological Microscopy

Author : Patrick Echlin
Publisher :
ISBN 13 :
Total Pages : 320 pages
Book Rating : 4.:/5 (31 download)

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Book Synopsis Low Temperature Biological Microscopy by : Patrick Echlin

Download or read book Low Temperature Biological Microscopy written by Patrick Echlin and published by . This book was released on 1977 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biological Field Emission Scanning Electron Microscopy, 2 Volume Set

Author : Roland A. Fleck
Publisher : John Wiley & Sons
ISBN 13 : 1118654064
Total Pages : 741 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck

Download or read book Biological Field Emission Scanning Electron Microscopy, 2 Volume Set written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Ultrastructure Techniques for Microorganisms

Author : H. C. Aldrich
Publisher :
ISBN 13 : 9781468451207
Total Pages : 552 pages
Book Rating : 4.4/5 (512 download)

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Book Synopsis Ultrastructure Techniques for Microorganisms by : H. C. Aldrich

Download or read book Ultrastructure Techniques for Microorganisms written by H. C. Aldrich and published by . This book was released on 1986-08-31 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Introduction to Analytical Electron Microscopy

Author : John Hren
Publisher : Springer Science & Business Media
ISBN 13 : 1475755813
Total Pages : 609 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Introduction to Analytical Electron Microscopy by : John Hren

Download or read book Introduction to Analytical Electron Microscopy written by John Hren and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: The birth of analytical electron microscopy (AEM) is somewhat obscure. Was it the recognition of the power and the development of STEM that signaled its birth? Was AEM born with the attachment of a crystal spectrometer to an otherwise conventional TEM? Or was it born earlier with the first analysis of electron loss spectra? It's not likely that any of these developments alone would have been sufficient and there have been many others (microdiffraction, EDS, microbeam fabrication, etc.) that could equally lay claim to being critical to the establishment of true AEM. It is probably more accurate to simply ascribe the present rapid development to the obvious: a combination of ideas whose time has come. Perhaps it is difficult to trace the birth of AEM simply because it remains a point of contention to even define its true scope. For example, the topics in this book, even though very broad, are still far from a complete description of what many call AEM. When electron beams interact with a solid it is well-known that a bewildering number of possible interactions follow. Analytical electron microscopy attempts to take full qualitative and quantitative advantage of as many of these interactions as possible while still preserving the capability of high resolution imaging. Although we restrict ourselves here to electron transparent films, much of what is described applies to thick specimens as well. Not surprisingly, signals from all possible interactions cannot yet (and probably never will) be attained simultaneously under optimum conditions.

Cryotechniques in Biological Electron Microscopy

Author : Rudolf A. Steinbrecht
Publisher : Springer Science & Business Media
ISBN 13 : 3642728154
Total Pages : 308 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Cryotechniques in Biological Electron Microscopy by : Rudolf A. Steinbrecht

Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Principles of Analytical Electron Microscopy

Author : Joseph Goldstein
Publisher : Springer Science & Business Media
ISBN 13 : 1489920374
Total Pages : 458 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Principles of Analytical Electron Microscopy by : Joseph Goldstein

Download or read book Principles of Analytical Electron Microscopy written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication in 1979 of Introduction to Analytical Electron Microscopy (ed. J. J. Hren, J. I. Goldstein, and D. C. Joy; Plenum Press), analytical electron microscopy has continued to evolve and mature both as a topic for fundamental scientific investigation and as a tool for inorganic and organic materials characterization. Significant strides have been made in our understanding of image formation, electron diffraction, and beam/specimen interactions, both in terms of the "physics of the processes" and their practical implementation in modern instruments. It is the intent of the editors and authors of the current text, Principles of Analytical Electron Microscopy, to bring together, in one concise and readily accessible volume, these recent advances in the subject. The text begins with a thorough discussion of fundamentals to lay a foundation for today's state-of-the-art microscopy. All currently important areas in analytical electron microscopy-including electron optics, electron beam/specimen interactions, image formation, x-ray microanalysis, energy-loss spectroscopy, electron diffraction and specimen effects-have been given thorough attention. To increase the utility of the volume to a broader cross section of the scientific community, the book's approach is, in general, more descriptive than mathematical. In some areas, however, mathematical concepts are dealt with in depth, increasing the appeal to those seeking a more rigorous treatment of the subject.

The Principles and Practice of Electron Microscopy

Author : Ian M. Watt
Publisher : Cambridge University Press
ISBN 13 : 9780521435918
Total Pages : 506 pages
Book Rating : 4.4/5 (359 download)

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Book Synopsis The Principles and Practice of Electron Microscopy by : Ian M. Watt

Download or read book The Principles and Practice of Electron Microscopy written by Ian M. Watt and published by Cambridge University Press. This book was released on 1997-01-30 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of this book was widely praised as an excellent introduction to electron microscopy for materials scientists, physicists, earth and biological scientists. This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practising electron microscopists. The coverage has been brought completely up to date, whilst retaining descriptions of early classic techniques. Currently live topics such as computer control of microscopes, energy-filtered imaging, cryo- and environmental microscopy, digital imaging, and high resolution scanning and transmission microscopy are all described. The highly praised case studies of the first edition have been expanded to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology.

Biological Low-Voltage Scanning Electron Microscopy

Author : James Pawley
Publisher : Springer Science & Business Media
ISBN 13 : 0387729720
Total Pages : 323 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Biological Low-Voltage Scanning Electron Microscopy by : James Pawley

Download or read book Biological Low-Voltage Scanning Electron Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2007-12-03 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Ultrastructure Techniques for Microorganisms

Author : H.C. Aldrich
Publisher : Springer Science & Business Media
ISBN 13 : 1468451197
Total Pages : 540 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Ultrastructure Techniques for Microorganisms by : H.C. Aldrich

Download or read book Ultrastructure Techniques for Microorganisms written by H.C. Aldrich and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.

Cumulated Index Medicus

Author :
Publisher :
ISBN 13 :
Total Pages : 1480 pages
Book Rating : 4.3/5 (243 download)

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Book Synopsis Cumulated Index Medicus by :

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1976 with total page 1480 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Advances in Low-Temperature Biology

Author : P.L. Steponkus
Publisher : Elsevier
ISBN 13 : 0080552285
Total Pages : 336 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Advances in Low-Temperature Biology by : P.L. Steponkus

Download or read book Advances in Low-Temperature Biology written by P.L. Steponkus and published by Elsevier. This book was released on 1996-12-17 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Low-Temperature Biology

Biomedical Electron Microscopy

Author : Arvid B. Maunsbach
Publisher : Elsevier
ISBN 13 : 0080528090
Total Pages : 569 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Biomedical Electron Microscopy by : Arvid B. Maunsbach

Download or read book Biomedical Electron Microscopy written by Arvid B. Maunsbach and published by Elsevier. This book was released on 1998-11-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs

DHHS Publication No. (NIH).

Author :
Publisher :
ISBN 13 :
Total Pages : 148 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis DHHS Publication No. (NIH). by :

Download or read book DHHS Publication No. (NIH). written by and published by . This book was released on 1987 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic Methods in Biological X-ray Microanalysis

Author : Godfried M. Roomans
Publisher :
ISBN 13 :
Total Pages : 292 pages
Book Rating : 4.:/5 (89 download)

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Book Synopsis Basic Methods in Biological X-ray Microanalysis by : Godfried M. Roomans

Download or read book Basic Methods in Biological X-ray Microanalysis written by Godfried M. Roomans and published by . This book was released on 1983 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Biotechnology Resources

Author :
Publisher :
ISBN 13 :
Total Pages : 88 pages
Book Rating : 4.:/5 (3 download)

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Book Synopsis Biotechnology Resources by :

Download or read book Biotechnology Resources written by and published by . This book was released on 1983 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: Directory of resources that serve the national biomedical community with new technologies and procedures. Arrangement according to category of resource service, i.e., Computer resources, Biomedical engineering resources, Biological structure and function, and Cellular and biochemical materials. Each entry gives title of resource, investigator, descriptions of equipment and personnel, objectives or applications, and current research. Geographical index.

Plant Nutrition — from Genetic Engineering to Field Practice

Author : J. Barrow
Publisher : Springer Science & Business Media
ISBN 13 : 9401118809
Total Pages : 787 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Plant Nutrition — from Genetic Engineering to Field Practice by : J. Barrow

Download or read book Plant Nutrition — from Genetic Engineering to Field Practice written by J. Barrow and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Nutrition - From Genetic Engineering to Field Practice, the 12th International Colloquium on Plant Nutrition, is the latest in a series which began in 1954. Early meetings were mainly concerned with the practical problems of soil fertility, with soil assessment, fertilizer requirements and methods of analysis. As the colloquia have progressed, the emphasis has slowly changed. The practical problems are still important, but there is increasing emphasis on plant physiology, plant biochemistry, membrane biochemistry, and even on the chemistry of genes which control the proteins which transfer nutrient ions to the inside of cells. The meetings therefore provide a valuable opportunity for each half of the science of plant nutrition to interact with, and learn from the other half. This volume begins with five papers which review current knowledge in important fields: the rhizosphere, molecular biology, electron microscopy, location and function of elements in vivo, and modelling nutrient responses in the field. These themes are continued in groups of shorter papers which follow. In addition, there are sections on nutrient dynamics and partitioning, diagnostic techniques, plant survival strategies, mycorrhizas, and on nutrients such as P, N, S, K, Ca, Mg, and micronutrients. A large section is devoted specifically to boron - reflecting the considerable current interest in this element. In total there are 177 refereed papers providing both a broad overview and a detailed picture of the latest developments in pure and applied plant nutrition.