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Laser Induced Fluorescence Detection In High Throughput Screening Of Heterogeneous Catalysts And Single Cells Analysis
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Book Synopsis Laser-induced Fluorescence Detection in High-throughput Screening of Heterogeneous Catalysts and Single Cells Analysis by : Hui Su
Download or read book Laser-induced Fluorescence Detection in High-throughput Screening of Heterogeneous Catalysts and Single Cells Analysis written by Hui Su and published by . This book was released on 2001 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to develop laser-induced fluorescence detection in high-throughput screening of heterogeneous catalysts and single cells analysis. First, a high-throughput in situ screening approach for solid heterogeneous catalysts by laser-induced fluorescence imaging is introduced. We demonstrated LIFI has good detection performance and the spatial and temporal resolution needed for high-throughput screening of heterogeneous catalysts. We further applied the LIFI screening system for combinatorial discovery of heterogeneous catalysts and reaction condition optimization for naphthalene oxidation, as well as combinatorial study of catalytic performance of zeolites in acylation of aromatics. In the second part of this dissertation, we used laser-induced native fluorescence coupled with capillary electrophoresis (LINF-CE) and microscope imaging to study the single cell degranulation. On the basis of good temporal correlation with events observed through an optical microscope, we have identified individual peaks in the fluorescence electropherograms as serotonin released from the granular core on contact with the surrounding fluid.
Book Synopsis Laser-Induced Fluorescence Detection in High-Throughput Screening of Heterogeneous Catalysts and Single Cells Analysis by :
Download or read book Laser-Induced Fluorescence Detection in High-Throughput Screening of Heterogeneous Catalysts and Single Cells Analysis written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser-induced fluorescence detection is one of the most sensitive detection techniques and it has found enormous applications in various areas. The purpose of this research was to develop detection approaches based on laser-induced fluorescence detection in two different areas, heterogeneous catalysts screening and single cell study. First, we introduced laser-induced imaging (LIFI) as a high-throughput screening technique for heterogeneous catalysts to explore the use of this high-throughput screening technique in discovery and study of various heterogeneous catalyst systems. This scheme is based on the fact that the creation or the destruction of chemical bonds alters the fluorescence properties of suitably designed molecules. By irradiating the region immediately above the catalytic surface with a laser, the fluorescence intensity of a selected product or reactant can be imaged by a charge-coupled device (CCD) camera to follow the catalytic activity as a function of time and space. By screening the catalytic activity of vanadium pentoxide catalysts in oxidation of naphthalene, we demonstrated LIFI has good detection performance and the spatial and temporal resolution needed for high-throughput screening of heterogeneous catalysts. The sample packing density can reach up to 250 x 250 subunits/cm2 for 40-[mu]m wells. This experimental set-up also can screen solid catalysts via near infrared thermography detection.
Book Synopsis Integrated Laser-Induced Fluorescence Detection for Parallelizable Microfluidic Single Cell Analysis by : Debbie Lo
Download or read book Integrated Laser-Induced Fluorescence Detection for Parallelizable Microfluidic Single Cell Analysis written by Debbie Lo and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic devices employing on-chip capillary electrophoresis (ÂľCE) have the potential to analyze single cells at high throughput while demanding less sample volume. However, to date, detecting the lower limit of the biological dynamic range involves coupling the fluorescence into confocal set-ups using externally cooled photomultiplier tubes (PMTs) which inhibit parallelization of the analysis. The integration of photodetector elements directly on-chip is desirable, but thus far, demonstrated systems are unable to detect targets without increasing target concentration by chemical techniques such as polyermase chain reaction (PCR). To address these demands, this work will investigate a laser-induced fluorescence (LIF) detection scheme using a high-index micro-ball lens embedded in a carbon-blackened polydimethylsiloxane (PDMS) panchromatic filter. The lens-filter layer enables simultaneous excitation rejection and fluorescence enhancement to couple fluorescence from a microfluidic channel to a surface detector. Furthermore, a method is proposed to optimize signal-to-noise ratio (SNR) in these higher dark current environments towards the goal of multiplexable and parallelizable integrated LIF detection.
Author :Radislav A. Potyrailo Publisher :Springer Science & Business Media ISBN 13 :1441989897 Total Pages :642 pages Book Rating :4.4/5 (419 download)
Book Synopsis High-Throughput Analysis by : Radislav A. Potyrailo
Download or read book High-Throughput Analysis written by Radislav A. Potyrailo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, edited by Potyrailo and Amis, addresses a new paradigm-shifting approach in the search for new materials-Combinatorial Materials Science. One way to consider such an approach is to imagine an adventurous chef who decides to look for new entrees by cooking food ingredients in many pots using different combinations in every pot, and boil ing, steaming, or frying them in various ways. Although most of the pots will not have the tastiest food ever devised, some recipes will taste intriguing, and some eventually will lead to a discovery of a new fascinating cuisine. Of course, having a skilled chef design the com binatorial formulation will certainly be helpful in ensuring a successful outcome. Similar to food, each engineering material is a complex product of its chemical composition, structure, and processing. Generally, each of these components matters---change one and you get another material. Most of these "new" materials will be less good than ones we use now since existing materials have been refined with the extensive work of scientists and engi neers. At the same time if one prepares diverse materials like our adventurous chef, chang ing material composition, processing conditions and time, etc. , some of these materials will be superior to existing ones and a few might represent breakout technology.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 994 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Laser Induced Fluorescence Detection in Biomedical Analysis by : John Emerson Kuo
Download or read book Applications of Laser Induced Fluorescence Detection in Biomedical Analysis written by John Emerson Kuo and published by . This book was released on 1985 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by :
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by and published by . This book was released on 1995 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Ph. D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 2000 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by : Qifeng Xue
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Commencement by : Iowa State University
Download or read book Commencement written by Iowa State University and published by . This book was released on 2001 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Laser induced fluorescence detection in high-performance liquid chromatography and its application in bioanalysis by : Cas van den Beld
Download or read book Laser induced fluorescence detection in high-performance liquid chromatography and its application in bioanalysis written by Cas van den Beld and published by . This book was released on 1991 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Visible Diode Laser Induced Fluorescence Detection in High Performance Liquid Chromatography by : Sadayappan Visswanathan Rahavendran
Download or read book Visible Diode Laser Induced Fluorescence Detection in High Performance Liquid Chromatography written by Sadayappan Visswanathan Rahavendran and published by . This book was released on 1996 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Directory of Graduate Research by : American Chemical Society. Committee on Professional Training
Download or read book Directory of Graduate Research written by American Chemical Society. Committee on Professional Training and published by . This book was released on 2005 with total page 1932 pages. Available in PDF, EPUB and Kindle. Book excerpt: Faculties, publications and doctoral theses in departments or divisions of chemistry, chemical engineering, biochemistry and pharmaceutical and/or medicinal chemistry at universities in the United States and Canada.
Book Synopsis Kirk-Othmer Encyclopedia of Chemical Technology, Volume 7 by : Kirk-Othmer
Download or read book Kirk-Othmer Encyclopedia of Chemical Technology, Volume 7 written by Kirk-Othmer and published by Wiley-Interscience. This book was released on 2004 with total page 900 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fifth edition of the Kirk-Othmer Encyclopedia of Chemical Technology builds upon the solid foundation of the previous editions, which have proven to be a mainstay for chemists, biochemists, and engineers at academic, industrial, and government institutions since publication of the first edition in 1949. The new edition includes necessary adjustments and modernization of the content to reflect changes and developments in chemical technology.
Book Synopsis Single Cell Analysis by : Tuhin Subhra Santra
Download or read book Single Cell Analysis written by Tuhin Subhra Santra and published by MDPI. This book was released on 2021-06-02 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Author :Radislav A. Potyrailo Publisher :Springer Science & Business Media ISBN 13 :0387737138 Total Pages :495 pages Book Rating :4.3/5 (877 download)
Book Synopsis Combinatorial Methods for Chemical and Biological Sensors by : Radislav A. Potyrailo
Download or read book Combinatorial Methods for Chemical and Biological Sensors written by Radislav A. Potyrailo and published by Springer Science & Business Media. This book was released on 2009-03-21 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical sensors are in high demand for applications as varied as water pollution detection, medical diagnostics, and battlefield air analysis. Designing the next generation of sensors requires an interdisciplinary approach. The book provides a critical analysis of new opportunities in sensor materials research that have been opened up with the use of combinatorial and high-throughput technologies, with emphasis on experimental techniques. For a view of component selection with a more computational perspective, readers may refer to the complementary volume of Integrated Analytical Systems edited by M. Ryan et al., entitled “Computational Methods for Sensor Material Selection”.
Book Synopsis Single Cell Protein Analysis by : Anup K. Singh
Download or read book Single Cell Protein Analysis written by Anup K. Singh and published by Humana. This book was released on 2015-11-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.
