Laser Imaging And Manipulation In Cell Biology

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Laser Imaging and Manipulation in Cell Biology

Author : Francesco S. Pavone
Publisher : John Wiley & Sons
ISBN 13 : 3527632255
Total Pages : 229 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Laser Imaging and Manipulation in Cell Biology by : Francesco S. Pavone

Download or read book Laser Imaging and Manipulation in Cell Biology written by Francesco S. Pavone and published by John Wiley & Sons. This book was released on 2011-07-28 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, the editor has gathered a team of international experts to present the latest advances in the field of laser imaging and manipulation techniques. The result is broad coverage of the interactions with biological samples to perform novel optical manipulation operations, both on the cellular and tissue levels. Of interest to physicists working and researching laser tissue mechanisms, cell biologists investigating new imaging and manipulation operation on the cellular level, medical doctors working with new laser therapies and diagnostic tools, as well as engineers developing new technologies in the field of optics and lasers.

Laser Manipulation of Cells and Tissues

Author : Michael W. Berns
Publisher : Academic Press
ISBN 13 :
Total Pages : 824 pages
Book Rating : 4.X/5 (3 download)

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Book Synopsis Laser Manipulation of Cells and Tissues by : Michael W. Berns

Download or read book Laser Manipulation of Cells and Tissues written by Michael W. Berns and published by Academic Press. This book was released on 2007-06-20 with total page 824 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. Laser Manipulation of Cells and Tissues ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one's own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology. Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers

Laser Tweezers in Cell Biology

Author :
Publisher : Academic Press
ISBN 13 : 9780080859538
Total Pages : 228 pages
Book Rating : 4.8/5 (595 download)

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Book Synopsis Laser Tweezers in Cell Biology by :

Download or read book Laser Tweezers in Cell Biology written by and published by Academic Press. This book was released on 1997-11-24 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer. * * Provides a practical laboratory guide for methods and technologies used with laser tweezers * Includes comprehensive and easy-to-follow protocols

Micromanipulation by Light in Biology and Medicine

Author : Karl Otto Greulich
Publisher : Springer Science & Business Media
ISBN 13 : 1461241103
Total Pages : 308 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Micromanipulation by Light in Biology and Medicine by : Karl Otto Greulich

Download or read book Micromanipulation by Light in Biology and Medicine written by Karl Otto Greulich and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are probably few people who do not dream of the good old times, when do ing science often meant fascination, excitement, even adventure. In our time, do ing science involves often technology and, perhaps, even business. But there are still niches where curiosity and fascination have their place. The subject of this book, technological as its title may sound, is one of the fortunate examples. It will report on lasers generating the coldest places in the Universe, and on table top laser microtools which can produce a heat "inferno" as it prevails in the interior of the Sun, or simulate, for specific plant cells, microgravity of the space around our plan et Earth. There will be some real surprises for the reader. The applications range from basic studies of the driving forces of cell division (and thus life) via genetic modification of cells (for example, for plant breeding) to medical applications such as blood cell analysis and finally in vitro fertilization. What are these instruments: laser microbeams and optical tweezers? Both are lasers coupled with a fluorescence microscope. The laser microbeam uses a pulsed ultraviolet laser. Light is focused, as well as possible, in space and time, in order to obtain extremely high light intensities - high enough to generate, for a very short instant, extremely hot spots which can be used to cut, fuse or perforate biological material.

Laser Manipulation of Cells and Tissues

Author : Michael W. Berns
Publisher : Academic Press
ISBN 13 : 9780123706485
Total Pages : 792 pages
Book Rating : 4.7/5 (64 download)

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Book Synopsis Laser Manipulation of Cells and Tissues by : Michael W. Berns

Download or read book Laser Manipulation of Cells and Tissues written by Michael W. Berns and published by Academic Press. This book was released on 2007-07-04 with total page 792 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. Laser Manipulation of Cells and Tissues ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one's own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology. Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers

Proceedings of Functional Imaging and Optical Manipulation of Living Cells

Author : International Biomedical Optics Society
Publisher : SPIE-International Society for Optical Engineering
ISBN 13 :
Total Pages : 152 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Proceedings of Functional Imaging and Optical Manipulation of Living Cells by : International Biomedical Optics Society

Download or read book Proceedings of Functional Imaging and Optical Manipulation of Living Cells written by International Biomedical Optics Society and published by SPIE-International Society for Optical Engineering. This book was released on 1997 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Pulsed Laser Microbeams for Cellular Manipulation

Author : Amy Noel Stacy Hellman
Publisher : ProQuest
ISBN 13 : 9780549605348
Total Pages : 174 pages
Book Rating : 4.6/5 (53 download)

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Book Synopsis Pulsed Laser Microbeams for Cellular Manipulation by : Amy Noel Stacy Hellman

Download or read book Pulsed Laser Microbeams for Cellular Manipulation written by Amy Noel Stacy Hellman and published by ProQuest. This book was released on 2008 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly focused pulsed laser radiation (pulsed laser microbeams) provide the ability to deposit energy with high spatial precision and controllable cellular damage. As a result, pulsed laser microbeams have been explored as a fast, non-contact means for cellular manipulations such as cellular microsurgery, transient cell membrane permeabilization, and targeted cell lysis. In this dissertation we examine the mechanisms of highly focused laser microbeams of nanosecond and picosecond duration to achieve cell lysis, cell necrosis, and molecular delivery. We have developed a time-resolved imaging system to visualize these processes with nanosecond temporal resolution and use image analysis to measure the physical perturbation applied to the cells as a function of laser microbeam pulse energy and pulse duration. Fluorescence assays are used to assess the biological response (necrosis, molecular delivery, and biochemical pathway activity) to the laser microbeam irradiation, and a biophysical model is developed to establish connections between specific physical characteristics and the resulting cellular effect. Our studies reveal that pulsed laser microbeam processes are mediated by optical breakdown resulting in plasma formation, shock wave emission, and cavitation bubble formation, expansion, and collapse. Cavitation bubble expansion was found to be the primary mechanism responsible for cellular modification. Hydrodynamic analysis based on the measured time evolution of the cavitation bubble growth, combined with assessment of the cellular response, revealed that the maximum wall shear stress associated with the cavitation bubble expansion governs the location and spatial extent of cell lysis, cell necrosis, and molecular delivery. In addition, we demonstrate how the variation of laser microbeam pulse duration can allow modulation of the spatial extent of cellular modification in order to tailor the cellular perturbations and optimize specific applications. These detailed studies provide a basis for the informed selection of specific laser parameters (i.e. pulse duration and energy) to achieve a desired cellular outcome, with controllable degrees of collateral damage. We demonstrate three different applications in which the laser parameters are well matched for particular applications in cellular analytics, activation of biochemical pathways, and microfluidics.

Lasers and Current Optical Techniques in Biology

Author : Giuseppe Palumbo
Publisher : Royal Society of Chemistry
ISBN 13 : 1847551203
Total Pages : 684 pages
Book Rating : 4.8/5 (475 download)

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Book Synopsis Lasers and Current Optical Techniques in Biology by : Giuseppe Palumbo

Download or read book Lasers and Current Optical Techniques in Biology written by Giuseppe Palumbo and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of innovative light sources, fibre laser sources and light emitting diodes, is opening unexpected perspectives into optical techniques and is promising new exciting applications in the field of biomedicine. Lasers and Current Optical Techniques in Biology aims to provide an overview of light sources, together with an extensive and authoritative description of the optical techniques in bio-medicine. This book is designed to give biomedical researchers a strong feel for the capability of physical approaches, promote new interdisciplinary interests and persuade more practitioners to take advantage of optical techniques. Current developments in a variety of optical techniques, including Near-Infra Red Spectroscopy, and traditional and advanced fluorescence techniques are covered, ranging from those that are becoming common practice to those that need much more experimentation before they can be accepted as real breakthroughs. Further topics include optical coherence tomography and its variations, polarised light imaging and, principle laser and lamp sources- a usually fragmentary topic, often dispersed among specialist publications. The wide range of topics covered make Lasers and Current Optical Techniques in Biology of interest to a diverse range of scientific communities.

Cell Imaging Techniques

Author : Douglas J. Taatjes
Publisher : Springer Science & Business Media
ISBN 13 : 1592599931
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Cell Imaging Techniques by : Douglas J. Taatjes

Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Author : Karsten König
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 311043007X
Total Pages : 450 pages
Book Rating : 4.1/5 (14 download)

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Book Synopsis Multiphoton Microscopy and Fluorescence Lifetime Imaging by : Karsten König

Download or read book Multiphoton Microscopy and Fluorescence Lifetime Imaging written by Karsten König and published by Walter de Gruyter GmbH & Co KG. This book was released on 2018-01-22 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Optical Imaging Techniques in Cell Biology

Author : Guy Cox
Publisher : CRC Press
ISBN 13 : 1420005618
Total Pages : 304 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Optical Imaging Techniques in Cell Biology by : Guy Cox

Download or read book Optical Imaging Techniques in Cell Biology written by Guy Cox and published by CRC Press. This book was released on 2006-11-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E

Emerging Tools for Single-Cell Analysis

Author : Gary Durack
Publisher : John Wiley & Sons
ISBN 13 : 0471461008
Total Pages : 375 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Emerging Tools for Single-Cell Analysis by : Gary Durack

Download or read book Emerging Tools for Single-Cell Analysis written by Gary Durack and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,

Confocal Microscopy

Author : Stephen W. Paddock
Publisher : Springer Science & Business Media
ISBN 13 : 159259722X
Total Pages : 446 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Confocal Microscopy by : Stephen W. Paddock

Download or read book Confocal Microscopy written by Stephen W. Paddock and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.

Advanced Biophotonics

Author : Ruikang K. Wang
Publisher : Taylor & Francis
ISBN 13 : 1439895821
Total Pages : 726 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Advanced Biophotonics by : Ruikang K. Wang

Download or read book Advanced Biophotonics written by Ruikang K. Wang and published by Taylor & Francis. This book was released on 2016-04-19 with total page 726 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and

Lasers for Medical Applications

Author : Helena Jelínková
Publisher : Elsevier
ISBN 13 : 0857097547
Total Pages : 832 pages
Book Rating : 4.8/5 (57 download)

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Book Synopsis Lasers for Medical Applications by : Helena Jelínková

Download or read book Lasers for Medical Applications written by Helena Jelínková and published by Elsevier. This book was released on 2013-09-30 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lasers have a wide and growing range of applications in medicine. Lasers for Medical Applications summarises the wealth of recent research on the principles, technologies and application of lasers in diagnostics, therapy and surgery. Part one gives an overview of the use of lasers in medicine, key principles of lasers and radiation interactions with tissue. To understand the wide diversity and therefore the large possible choice of these devices for a specific diagnosis or treatment, the respective types of the laser (solid state, gas, dye, and semiconductor) are reviewed in part two. Part three describes diagnostic laser methods, for example optical coherence tomography, spectroscopy, optical biopsy, and time-resolved fluorescence polarization spectroscopy. Those methods help doctors to refine the scope of involvement of the particular body part or, for example, to specify the extent of a tumor. Part four concentrates on the therapeutic applications of laser radiation in particular branches of medicine, including ophthalmology, dermatology, cardiology, urology, gynecology, otorhinolaryngology (ORL), neurology, dentistry, orthopaedic surgery and cancer therapy, as well as laser coatings of implants. The final chapter includes the safety precautions with which the staff working with laser instruments must be familiar. With its distinguished editor and international team of contributors, this important book summarizes international achievements in the field of laser applications in medicine in the past 50 years. It provides a valuable contribution to laser medicine by outstanding experts in medicine and engineering. Describes the interaction of laser light with tissue Reviews every type of laser used in medicine: solid state, gas, dye and semiconductor Describes the use of lasers for diagnostics

Confocal Laser Scanning Microscopy

Author : C. Sheppard
Publisher : BIOS Scientific Publishers
ISBN 13 : 9780387915142
Total Pages : 122 pages
Book Rating : 4.9/5 (151 download)

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Book Synopsis Confocal Laser Scanning Microscopy by : C. Sheppard

Download or read book Confocal Laser Scanning Microscopy written by C. Sheppard and published by BIOS Scientific Publishers. This book was released on 1997-09-26 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the principles of the technique and illustrates applications in physical and biomedical sciences. Covers Image formation in confocal microscopy, Performance of the confocal microscope, Biological and industrial applications. Paper. DLC: Confocal microscopy.

Robotic Cell Manipulation

Author : Dong Sun
Publisher : Academic Press
ISBN 13 : 0323852602
Total Pages : 548 pages
Book Rating : 4.3/5 (238 download)

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Book Synopsis Robotic Cell Manipulation by : Dong Sun

Download or read book Robotic Cell Manipulation written by Dong Sun and published by Academic Press. This book was released on 2022-05-31 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: Robotic Cell Manipulation introduces up-to-date research to realize this new theme of medical robotics. The book is organized in three levels: operation tools (e.g., optical tweezers, microneedles, dielectrophoresis, electromagnetic devices, and microfluidic chips), manipulation types (e.g., microinjection, transportation, rotation fusion, adhesion, separation, etc.), and potential medical applications (e.g., micro-surgery, biopsy, gene editing, cancer treatment, cell-cell interactions, etc.). The technology involves different fields such as robotics, automation, imaging, microfluidics, mechanics, materials, biology and medical sciences. The book provides systematic knowledge on the subject, covering a wide range of basic concepts, theories, methodology, experiments, case studies and potential medical applications. It will enable readers to promptly conduct a systematic review of research and become an essential reference for many new and experienced researchers entering this unique field. Introduces the applications of robot-assisted manipulation tools in various cell manipulation tasks Defines many essential concepts in association with the robotic cell manipulation field, including manipulation strategy and manipulation types Introduces basic concepts and knowledge on various manipulation devices and tasks Describes some cutting-edge cell manipulation technologies and case studies