Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Laser Desorption Mass Spectrometry For Dna Analysis And Sequencing
Download Laser Desorption Mass Spectrometry For Dna Analysis And Sequencing full books in PDF, epub, and Kindle. Read online Laser Desorption Mass Spectrometry For Dna Analysis And Sequencing ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing by :
Download or read book Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing written by and published by . This book was released on 1995 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser desorption mass spectrometry has been considered as a potential new method for fast DNA sequencing. Our approach is to use matrix-assisted laser desorption to produce parent ions of DNA segments and a time-of-flight mass spectrometer to identify the sizes of DNA segments. Thus, the approach is similar to gel electrophoresis sequencing using Sanger's enzymatic method. However, gel, radioactive tagging, and dye labeling are not required. In addition, the sequencing process can possibly be finished within a few hundred microseconds instead of hours and days. In order to use mass spectrometry for fast DNA sequencing, the following three criteria need to be satisfied. They are (1) detection of large DNA segments, (2) sensitivity reaching the femtomole region, and (3) mass resolution good enough to separate DNA segments of a single nucleotide difference. It has been very difficult to detect large DNA segments by mass spectrometry before due to the fragile chemical properties of DNA and low detection sensitivity of DNA ions. We discovered several new matrices to increase the production of DNA ions. By innovative design of a mass spectrometer, we can increase the ion energy up to 45 KeV to enhance the detection sensitivity. Recently, we succeeded in detecting a DNA segment with 500 nucleotides. The sensitivity was 100 femtomole. Thus, we have fulfilled two key criteria for using mass spectrometry for fast DNA sequencing. The major effort in the near future is to improve the resolution. Different approaches are being pursued. When high resolution of mass spectrometry can be achieved and automation of sample preparation is developed, the sequencing speed to reach 500 megabases per year can be feasible.
Book Synopsis Laser Desorption Mass Spectrometry for High Throughput DNA Analysis and Its Applications by :
Download or read book Laser Desorption Mass Spectrometry for High Throughput DNA Analysis and Its Applications written by and published by . This book was released on 1999 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laser desorption mass spectrometry (LDMS) has been developed for DNA sequencing, disease diagnosis, and DNA Fingerprinting for forensic applications. With LDMS, the speed of DNA analysis can be much faster than conventional gel electrophoresis. No dye or radioactive tagging to DNA segments for detection is needed. LDMS is emerging as a new alternative technology for DNA analysis.
Book Synopsis Laser Desorption Mass Spectrometry for Fast DNA Analysis by :
Download or read book Laser Desorption Mass Spectrometry for Fast DNA Analysis written by and published by . This book was released on 1995 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past few years, major effort has been directed toward developing mass spectrometry to measure biopolymers because of the great potential benefit to biomedical research. Hellenkamp and his co-workers were the first to report that large polypeptide molecules can be ionized and detected without significant fragmentation when a greater number of nicotinic acid molecules are used as a matrix. This method is now well known as matrix-assisted laser desorption/ionization (MALDI). Since then, various groups have reported measurements of very large proteins by MALDI. Reliable protein analysis by MALDI is more or less well established. However, the application of MALDI to nucleic acids analysis has been found to be much more difficult. Most research on the measurement of nucleic acid by MALDI were stimulated by the Human Genome Project. Up to now, the only method for reliable routine analysis of nucleic acid is gel electrophoresis. Different sizes of nucleic acids can be separated in gel medium when a high electric field is applied to the gel. However, the time needed to separate different sizes of DNA segments usually takes from several minutes to several hours. If MALDI can be successfully used for nucleic acids analysis, the analysis time can be reduced to less than I millisecond. In addition, no tagging with radioactive materials or chemical dyes is needed. In this work, we will review recent progress related to MALDI for DNA analysis.
Book Synopsis The Analysis of Nucleic Acids by Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry by : Michael C. FitzGerald
Download or read book The Analysis of Nucleic Acids by Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry written by Michael C. FitzGerald and published by . This book was released on 1994 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Genetic Analysis by Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry by : Timothy J. Griffin
Download or read book Genetic Analysis by Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry written by Timothy J. Griffin and published by . This book was released on 1999 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 by :
Download or read book High Speed DNA Sequence Analysis by Matrix-assisted Laser Desorption Mass Spectrometry. Final Report for Period February 15, 1991 - February 14, 2001 written by and published by . This book was released on 2001 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.
Book Synopsis Optimization of DNA Sequencing Using Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry with Biotinylated DdNTPs by : Michael E. Ulz
Download or read book Optimization of DNA Sequencing Using Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry with Biotinylated DdNTPs written by Michael E. Ulz and published by . This book was released on 2002 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fragmentation Mechanism and Sequence Analysis of Nucleic Acids by Matrix-assisted Laser Desorption/ionization Mass Spectrometry by : Lin Zhu
Download or read book Fragmentation Mechanism and Sequence Analysis of Nucleic Acids by Matrix-assisted Laser Desorption/ionization Mass Spectrometry written by Lin Zhu and published by . This book was released on 1995 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Single Nucleotide Polymorphism Analysis by Matrix-assisted Laser Desorption/ Ionization Time-of-flight Mass Spectrometry by : Zhengdong Fei
Download or read book Single Nucleotide Polymorphism Analysis by Matrix-assisted Laser Desorption/ Ionization Time-of-flight Mass Spectrometry written by Zhengdong Fei and published by . This book was released on 1999 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Suite de la relation de la Bataille de Bergben, remportée par les troupes du Roi, commandées par M. le Duc De Broglie. Du 19 Avril 1759 by :
Download or read book Suite de la relation de la Bataille de Bergben, remportée par les troupes du Roi, commandées par M. le Duc De Broglie. Du 19 Avril 1759 written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mass Spectrometry in Biopolymer Analysis by : Xiaoyu Chen
Download or read book Mass Spectrometry in Biopolymer Analysis written by Xiaoyu Chen and published by . This book was released on 2003 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analytical Techniques In DNA Sequencing by : Brian K. Nunnally
Download or read book Analytical Techniques In DNA Sequencing written by Brian K. Nunnally and published by CRC Press. This book was released on 2005-06-23 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: While one marvels at the DNA generated by the Human Genome Project, perhaps just as significant and fascinating is the development of the amazing analytical technology that has permitted us entry into the genomic era and a whole new level of scientific understanding. Analytical Techniques in DNA Sequencing takes a look at the various DNA sequencing techniques that were developed and put to use during and after the Human Genome Project. Nunnally makes a special effort to trace the development of this branch of research since the early days of radioisotope sequencing. Special attention is paid to the breakthrough Sanger Method (1977), as well as to several other techniques that improved the accuracy, improved the detection limits, and in a variety of ways dramatically reduced the time needed to generate a DNA sequence. This comprehensive volume describes the improvements in DNA separation based on advanced capillary electrophoresis and microchip sequencing. It also looks at the use of mass spectrometry and single molecule detection, and takes an in-depth look at the use of various fluorescence methods for the detection of sequencing fragments. One chapter is dedicated to chip-based microfabricated sequencing systems, which could well be the future of DNA sequencing. The book’s ten chapters, authored by a variety of leading experts in the field, include many of the far-reaching applications of DNA sequencing. Entire chapters are dedicated to DNA typing for forensics, a reevaluation of ancient DNA, and genome analysis.
Book Synopsis Laser Mass Spectrometry for DNA Fingerprinting for Forensic Applications by :
Download or read book Laser Mass Spectrometry for DNA Fingerprinting for Forensic Applications written by and published by . This book was released on 1994 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of DNA fingerprinting has become very broad in forensic analysis, patient identification, diagnostic medicine, and wildlife poaching, since every individual's DNA structure is identical within all tissues of their body. DNA fingerprinting was initiated by the use of restriction fragment length polymorphisms (RFLP). In 1987, Nakamura et al. found that a variable number of tandem repeats (VNTR) often occurred in the alleles. The probability of different individuals having the same number of tandem repeats in several different alleles is very low. Thus, the identification of VNTR from genomic DNA became a very reliable method for identification of individuals. DNA fingerprinting is a reliable tool for forensic analysis. In DNA fingerprinting, knowledge of the sequence of tandem repeats and restriction endonuclease sites can provide the basis for identification. The major steps for conventional DNA fingerprinting include (1) specimen processing (2) amplification of selected DNA segments by PCR, and (3) gel electrophoresis to do the final DNA analysis. In this work we propose to use laser desorption mass spectrometry for fast DNA fingerprinting. The process and advantages are discussed.
Book Synopsis Mass Spectrometry and Genomic Analysis by : J.N. Housby
Download or read book Mass Spectrometry and Genomic Analysis written by J.N. Housby and published by Springer Science & Business Media. This book was released on 2005-12-20 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: 76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3.
Book Synopsis Protein Sequencing and Identification Using Tandem Mass Spectrometry by : Michael Kinter
Download or read book Protein Sequencing and Identification Using Tandem Mass Spectrometry written by Michael Kinter and published by John Wiley & Sons. This book was released on 2005-04-12 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more
Book Synopsis Biological Identification by : R. Paul Schaudies
Download or read book Biological Identification written by R. Paul Schaudies and published by Elsevier. This book was released on 2014-05-08 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Identification provides a detailed review of, and potential future developments in, the technologies available to counter the threats to life and health posed by natural pathogens, toxins, and bioterrorism agents. Biological identification systems must be fast, accurate, reliable, and easy to use. It is also important to employ the most suitable technology in dealing with any particular threat. This book covers the fundamentals of these vital systems and lays out possible advances in the technology. Part one covers the essentials of DNA and RNA sequencing for the identification of pathogens, including next generation sequencing (NGS), polymerase chain reaction (PCR) methods, isothermal amplification, and bead array technologies. Part two addresses a variety of approaches to making identification systems portable, tackling the special requirements of smaller, mobile systems in fluid movement, power usage, and sample preparation. Part three focuses on a range of optical methods and their advantages. Finally, part four describes a unique approach to sample preparation and a promising approach to identification using mass spectroscopy. Biological Identification is a useful resource for academics and engineers involved in the microelectronics and sensors industry, and for companies, medical organizations and military bodies looking for biodetection solutions. Covers DNA sequencing of pathogens, lab-on-chip, and portable systems for biodetection and analysis Provides an in-depth description of optical systems and explores sample preparation and mass spectrometry-based biological analysis
Book Synopsis Matrix-assisted Laser Desorption/ionization Mass Spectrometric Study of Biologic Molecules by : Yan-Hui Liu
Download or read book Matrix-assisted Laser Desorption/ionization Mass Spectrometric Study of Biologic Molecules written by Yan-Hui Liu and published by . This book was released on 1996 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
