Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
In Vivo Cofactor Biosynthesis And Maintenance In The Class Ia Ribonucleotide Reductase Small Subunit Of Escherichia Coli
Download In Vivo Cofactor Biosynthesis And Maintenance In The Class Ia Ribonucleotide Reductase Small Subunit Of Escherichia Coli full books in PDF, epub, and Kindle. Read online In Vivo Cofactor Biosynthesis And Maintenance In The Class Ia Ribonucleotide Reductase Small Subunit Of Escherichia Coli ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis In Vivo Cofactor Biosynthesis and Maintenance in the Class Ia Ribonucleotide Reductase Small Subunit of Escherichia Coli by : Chia-Hung Wu (Ph. D.)
Download or read book In Vivo Cofactor Biosynthesis and Maintenance in the Class Ia Ribonucleotide Reductase Small Subunit of Escherichia Coli written by Chia-Hung Wu (Ph. D.) and published by . This book was released on 2009 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The small subunit ([beta]2) of Escherichia coli class Ia ribonucleotide reductases (RNRs) contains a diferric tyrosyl radical (Y*) cofactor essential for the conversion of nucleotides to deoxynucleotides that are needed for DNA synthesis and repair. The mechanism and factors involved in the biosynthesis, maintenance and regulation of this cluster remains unclear. To understand these pathways, the genes contiguous to nrdB (gene encoding [beta]) in 181 bacterial genomes were analyzed which revealed a highly conserved [2Fe2S]-ferredoxin, YfaE in E. coli. YfaE has been cloned, expressed, reconstituted, and characterized by UV-visible, EPR and Mössbauer spectroscopies. Titration of met-[beta]2 (an inactive diferric-[beta]2 with Y* reduced) with [2Fe2S]1+-YfaE results in formation of diferrous-[beta]2 with one Fe reduced/YfaE oxidized. At the end point of titration, exposure of the reduced cluster to O2 in the absence of an additional reducing equivalent yields the diferric-Y* with 2 Fe/Y* generated, suggesting that the reducing equivalent required for cluster assembly is supplied by [beta]2, likely by W48. The kobs for the reaction between met-[beta]2 and [2Fe2S]1+-YfaE determined by anaerobic stopped flow spectroscopy is ~1-5 s-1. Studies of conserved Lys to Ala mutations of [beta]2 indicate electrostatic interactions may play an important role for interaction with YfaE. Quantitative Western blots of the whole cells suggest that YfaE acts catalytically in reactivating met-[beta]2 in vivo. Titration experiments establish that met-[beta]2 can be reduced by catalytic amounts of YfaE, Fre (a flavin reductase) and flavin with consumption of NADPH. In the presence of a Y* scavenger, hydroxyurea, [delta]yfaE shows slower growth rates than the isogenic wt strain and Western blots analysis shows up-regulation of YfaE expression, supporting YfaE's role in the reactivation of diferric-[beta]2 in vivo. To investigate the iron sources for diferric-Y* assembly, changes in Fe pools inside the cell subsequent to expression of [beta]2 was monitored by whole cell Mössbauer spectroscopy. The results show that both Fe2+ and Fe3+ pools can provide the iron for cluster assembly, suggesting a reduction mechanism(s) for Fe3+ to allow it function in this capacity. A potential role of YfaE as an iron chaperone for iron delivery to [beta]2 has also been investigated.
Book Synopsis Regulation of Ribonucleotide Reductase MRNA Synthesis in Escherichia Coli by : Christopher Keith Tuggle
Download or read book Regulation of Ribonucleotide Reductase MRNA Synthesis in Escherichia Coli written by Christopher Keith Tuggle and published by . This book was released on 1986 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cofactor Biosynthesis: A Mechanistic Perspective by :
Download or read book Cofactor Biosynthesis: A Mechanistic Perspective written by and published by Academic Press. This book was released on 2001-01-02 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The longest running serial published by Academic Press continues its well-respected run with Volume 61, a special volume in which a guest editor has come on board and has assembled some well-known contributors who are international authorities in the field. Together they tackle some of the latest topics in the field such as riboflavin and folate biosynthesis, biotin and lipoic acid biosynthesis, nicotinamide adenine dinucleotide biosynthesis, biosynthesis of vitamin B6 and structurally related derivatives, pantothenic acid and coenzyme A biosynthesis, mechanistic biosynthesis of protein-derived redox cofactors, ascorbic acid biosynthesis, biosynthesis of menaquinone and ubiquinone - Vitamin B12 biosynthesis, biosynthesis of the methanogenic cofactors, and thiamin biosynthesis.
Book Synopsis The iron centre of ribonucleotide reductase protein R2 of Escherichia coli : effects of protein engineering of the iron ligands by : Bert Ove Persson
Download or read book The iron centre of ribonucleotide reductase protein R2 of Escherichia coli : effects of protein engineering of the iron ligands written by Bert Ove Persson and published by . This book was released on 1997 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Novel Cofactors by : Judith P. Klinman
Download or read book Novel Cofactors written by Judith P. Klinman and published by Gulf Professional Publishing. This book was released on 2001-10-05 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: A cofactor is a component part of many enzymes and functions by uniting with another molecule in order to become active. The use of cofactors to supplement the native amino acids of a protein is essential to maintain the chemical capabilities necessary for organisms to survive. This volume focuses on the significant advances of the past decade in identifying and describing new cofactors--either small molecules or those derived posttranslationally.
Book Synopsis Prokaryotic Metabolism and Physiology by : Byung Hong Kim
Download or read book Prokaryotic Metabolism and Physiology written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2019-05-16 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive and up-to-date review of key metabolic processes in bacteria and archaea and how metabolism is regulated under various conditions.
Book Synopsis Mitochondria and Anaerobic Energy Metabolism in Eukaryotes by : William F. Martin
Download or read book Mitochondria and Anaerobic Energy Metabolism in Eukaryotes written by William F. Martin and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-12-07 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondria are sometimes called the powerhouses of eukaryotic cells, because mitochondria are the site of ATP synthesis in the cell. ATP is the universal energy currency, it provides the power that runs all other life processes. Humans need oxygen to survive because of ATP synthesis in mitochondria. The sugars from our diet are converted to carbon dioxide in mitochondria in a process that requires oxygen. Just like a fire needs oxygen to burn, our mitochondria need oxygen to make ATP. From textbooks and popular literature one can easily get the impression that all mitochondria require oxygen. But that is not the case. There are many groups of organismsm known that make ATP in mitochondria without the help of oxygen. They have preserved biochemical relicts from the early evolution of eukaryotic cells, which took place during times in Earth history when there was hardly any oxygen avaiable, certainly not enough to breathe. How the anaerobic forms of mitochondria work, in which organisms they occur, and how the eukaryotic anaerobes that possess them fit into the larger picture of rising atmospheric oxygen during Earth history are the topic of this book.
Book Synopsis Bacterial Physiology and Metabolism by : Byung Hong Kim
Download or read book Bacterial Physiology and Metabolism written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2008-02-21 with total page 934 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent determination of genome sequences for a wide range of bacteria has made in-depth knowledge of prokaryotic metabolic function essential in order to give biochemical, physiological, and ecological meaning to the genomic information. Clearly describing the important metabolic processes that occur in prokaryotes under different conditions and in different environments, this advanced text provides an overview of the key cellular processes that determine bacterial roles in the environment, biotechnology, and human health. Prokaryotic structure is described as well as the means by which nutrients are transported into cells across membranes. Glucose metabolism through glycolysis and the TCA cycle are discussed, as well as other trophic variations found in prokaryotes, including the use of organic compounds, anaerobic fermentation, anaerobic respiratory processes, and photosynthesis. The regulation of metabolism through control of gene expression and control of the activity of enzymes is also covered, as well as survival mechanisms used under starvation conditions.
Book Synopsis Molecular Biology and Pathogenicity of Mycoplasmas by : Shmuel Razin
Download or read book Molecular Biology and Pathogenicity of Mycoplasmas written by Shmuel Razin and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: was the result of the efforts of Robert Cleverdon. The rapidly developing discipline of molecular biology and the rapidly expanding knowledge of the PPLO were brought together at this meeting. In addition to the PPLO specialists, the conference invited Julius Marmur to compare PPLO DNA to DNA of other organisms; David Garfinkel, who was one of the first to develop computer models of metabolism; Cyrus Levinthal to talk about coding; and Henry Quastler to discuss information theory constraints on very small cells. The conference was an announcement of the role of PPLO in the fundamental understanding of molecular biology. Looking back 40-some years to the Connecticut meeting, it was a rather bold enterprise. The meeting was international and inter-disciplinary and began a series of important collaborations with influences resonating down to the present. If I may be allowed a personal remark, it was where I first met Shmuel Razin, who has been a leading figure in the emerging mycoplasma research and a good friend. This present volume is in some ways the fulfillment of the promise of that early meeting. It is an example of the collaborative work of scientists in building an understanding of fundamental aspects of biology.
Book Synopsis Hydroxamic Acids by : Satya P. Gupta
Download or read book Hydroxamic Acids written by Satya P. Gupta and published by Springer Science & Business Media. This book was released on 2013-09-30 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Satya P. Gupta's Hydroxamics Acids is the first book to compile invited articles written by international experts on the class of compounds hydroxamic acids. Found to possess a wide spectrum of biological activities, the hydroxamic acids are of interest to theoretical and experimental chemists who can study and make use of them in drug design and development. Chapters in this book provide a diverse and comprehensive coverage of this compound class and consequently this publication is a valuable resource for researchers in chemical, pharmaceutical and biological sciences.
Book Synopsis Medicinal Chemistry of Anticancer Drugs by : Carmen Avendaño
Download or read book Medicinal Chemistry of Anticancer Drugs written by Carmen Avendaño and published by Elsevier. This book was released on 2015-06-11 with total page 767 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicinal Chemistry of Anticancer Drugs, Second Edition, provides an updated treatment from the point of view of medicinal chemistry and drug design, focusing on the mechanism of action of antitumor drugs from the molecular level, and on the relationship between chemical structure and chemical and biochemical reactivity of antitumor agents. Antitumor chemotherapy is a very active field of research, and a huge amount of information on the topic is generated every year. Cytotoxic chemotherapy is gradually being supplemented by a new generation of drugs that recognize specific targets on the surface or inside cancer cells, and resistance to antitumor drugs continues to be investigated. While these therapies are in their infancy, they hold promise of more effective therapies with fewer side effects. Although many books are available that deal with clinical aspects of cancer chemotherapy, this book provides a sorely needed update from the point of view of medicinal chemistry and drug design. Presents information in a clear and concise way using a large number of figures Historical background provides insights on how the process of drug discovery in the anticancer field has evolved Extensive references to primary literature
Book Synopsis Chemistry and Biochemistry of B12 by : Ruma Banerjee
Download or read book Chemistry and Biochemistry of B12 written by Ruma Banerjee and published by John Wiley & Sons. This book was released on 1999-11-03 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Definitive New Reference for the Latest Advances in B_12 Chemistry and Biochemistry Over the past decade, the field of B_12 research has been revolutionized by such major breakthroughs as the unraveling of the entire biosynthetic pathway for this important vitamin. This comprehensive compendium surveys the wealth of information that has accumulated, covering in one volume virtually all aspects of the field-from physical and inorganic chemistry to enzymology, microbiology, medicine, and diagnostic and therapeutic applications. Edited by Dr. Ruma Banerjee, a highly respected and active member of the B_12 community, this work provides B_12 researchers with a dependable and up-to-date reference on the subject. Leading authorities from five continents explore such new areas as the structural biology of B_12-dependent enzymes, free-radical-mediated reaction mechanisms, biosynthesis, and much more. The role of B_12 in nutrition and disease, and B_12 transport, are also thoroughly examined. Complete with color illustrations and extensive references, Chemistry and Biochemistry of B_12 is a one-of-a-kind resource for biochemists, biophysicists, spectroscopists, microbiologists, molecular biologists, and anyone with an interest in "nature's most beautiful cofactor."
Book Synopsis Nucleotide Metabolism by : J. Frank Henderson
Download or read book Nucleotide Metabolism written by J. Frank Henderson and published by Academic Press. This book was released on 2014-06-28 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleotide Metabolism: An Introduction is a textbook exclusively focusing on the study of the aspects of nucleotide metabolism. The book intends to present the chemistry and metabolism of nucleotides, one of the oldest subjects of biochemistry. The text is divided in two parts. Part I considers the general aspects of nucleotide metabolism such as the history of the discovery of nucleotides; functions of nucleotides in cells; and group-transfer reactions during nucleotide metabolism. Part II deals with the synthesis, formation, and conversion of purine ribonucleotides. Biochemists, pharmacologists, and researchers in the fields of medicine and pharmaceuticals will find the book invaluable.
Download or read book Biochemistry written by David E. Metzler and published by Elsevier. This book was released on 2003-05-04 with total page 1063 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry: The Chemical Reactions of Living Cells is a well-integrated, up-to-date reference for basic chemistry and underlying biological phenomena. Biochemistry is a comprehensive account of the chemical basis of life, describing the amazingly complex structures of the compounds that make up cells, the forces that hold them together, and the chemical reactions that allow for recognition, signaling, and movement. This book contains information on the human body, its genome, and the action of muscles, eyes, and the brain. * Thousands of literature references provide introduction to current research as well as historical background * Contains twice the number of chapters of the first edition * Each chapter contains boxes of information on topics of general interest
Book Synopsis Desk Encyclopedia of Microbiology by : Moselio Schaechter
Download or read book Desk Encyclopedia of Microbiology written by Moselio Schaechter and published by Academic Press. This book was released on 2010-04-19 with total page 1277 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Desk Encyclopedia of Microbiology, Second Edition is a single-volume comprehensive guide to microbiology for the advanced reader. Derived from the six volume e-only Encyclopedia of Microbiology, Third Edition, it bridges the gap between introductory texts and specialized reviews. Covering topics ranging from the basic science of microbiology to the current "hot" topics in the field, it will be invaluable for obtaining background information on a broad range of microbiological topics, preparing lectures and preparing grant applications and reports. The most comprehensive single-volume source providing an overview of microbiology to non-specialists Bridges the gap between introductory texts and specialized reviews Provides concise and general overviews of important topics within the field making it a helpful resource when preparing for lectures, writing reports, or drafting grant applications
Download or read book Carotenoids written by John T. Landrum and published by CRC Press. This book was released on 2009-12-21 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carotenoids are of great interest due to their essential biological functions in both plants and animals. However, the properties and functions of carotenoids in natural systems are surprisingly complex. With an emphasis on the chemical aspects of these compounds, Carotenoids: Physical, Chemical, and Biological Functions and Properties presents a b
Book Synopsis Essentials of Biochemistry by : Herbert J. Fromm
Download or read book Essentials of Biochemistry written by Herbert J. Fromm and published by Springer Science & Business Media. This book was released on 2012-01-05 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook, Essentials of Biochemistry is aimed at chemistry and biochemistry undergraduate students and first year biochemistry graduate students. It incorporates the lectures of the authors given to students with a strong chemistry background. An emphasis is placed on metabolism and reaction mechanisms and how they are studied. As the title of the book implies, the text lays the basis for an understanding of the fundamentals of biochemistry.
