Implications des harpines HrpNea et HrpWea, protéines de pathogénie de la bactérie Erwinia amylovora dans les étapes précoces d'interaction avec la cellule végétale

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Total Pages : 282 pages
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Book Synopsis Implications des harpines HrpNea et HrpWea, protéines de pathogénie de la bactérie Erwinia amylovora dans les étapes précoces d'interaction avec la cellule végétale by : David Reboutier

Download or read book Implications des harpines HrpNea et HrpWea, protéines de pathogénie de la bactérie Erwinia amylovora dans les étapes précoces d'interaction avec la cellule végétale written by David Reboutier and published by . This book was released on 2006 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harpins are protean effectors secreted by type III secretion system of phytopathogenic bacteria. The aim of the present work was to investigate the involvement of HrpNea and HrpWea harpins from Erwinia amylovora during the interaction between this bacteria and the plant cell. We first worked with suspension cells from the non-host plant Arabidopsis thaliana (incompatible situation). On this model, we showed that anion channel inhibition is a determinant event leading to cell death triggered by HrpNea. We then studied the effects of HrpWea. Our results showed that HrpWea has different effects depending on its concentration. At “high concentration”, HrpWea induced cell death, whereas at “low concentration”, HrpWea was able to inhibit HrpNea induced cell death. Anion channel modulation also appeared to be a determinant event of cell death inhibition. More widely, these results suggested that HrpWea could be a plant cell defense mechanism inhibitor. Finally, we studied the effects of HrpNea on the host apple cells (compatible situation). We showed differential responses between host and non-host plant cells, likely explaining that HrpNea did not trigger cell death in host plants.Taken together, these data allowed us to improve our knowledge concerning involvement of HrpNea and HrpWea in E. amylovora hypersensitive response and pathogenicity. Moreover, these results bring new data concerning the role of ion channels during cell death.