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Immunohistochemistry Basics And Methods
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Book Synopsis Immunohistochemistry: Basics and Methods by : Igor B. Buchwalow
Download or read book Immunohistochemistry: Basics and Methods written by Igor B. Buchwalow and published by Springer Science & Business Media. This book was released on 2010-01-26 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Book Synopsis Immunohistochemistry by : Alexander E. Kalyuzhny
Download or read book Immunohistochemistry written by Alexander E. Kalyuzhny and published by Springer. This book was released on 2016-03-24 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.
Download or read book Biobanking written by William H. Yong and published by Humana Press. This book was released on 2018-12-14 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.
Book Synopsis Immunohistochemistry by : Simon Renshaw
Download or read book Immunohistochemistry written by Simon Renshaw and published by Scion Publishing Ltd. This book was released on 2006-12-20 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. Introduction (S Renshaw) 2. Antibodies for immunochemistry (C Onley) 3. The selection of reporter labels (S Mardle) 4. Immunochemical staining techniques (S Renshaw) 5. Multiple immunochemical staining techniques (I Jones) 6. Confocal microscopy and immunohistochemistry (M Cuttle) 7. Ultrastructural immunochemistry (J Skepper) 8. Image capture, analysis and quantification (D Tannahill) 9. Quality assurance in immunohistochemistry (P Jackson) 10. Automated immunochemistry (E Schenck) List of suppliers; Index
Book Synopsis Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas by : M. A. Hayat
Download or read book Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas written by M. A. Hayat and published by Elsevier. This book was released on 2006-05-23 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. * The only book available that translates molecular genetics into cancer diagnosis * Methods were developed by internationally-recognized experts and presented in step-by-step manner * Results of each Immunohistochemical and in situ hybridization are presented in the form of color illustrations
Book Synopsis Bancroft's Theory and Practice of Histological Techniques E-Book by : Kim S Suvarna
Download or read book Bancroft's Theory and Practice of Histological Techniques E-Book written by Kim S Suvarna and published by Elsevier Health Sciences. This book was released on 2012-10-01 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.
Book Synopsis Immunocytochemical Methods and Protocols by : Constance Oliver
Download or read book Immunocytochemical Methods and Protocols written by Constance Oliver and published by Humana Press. This book was released on 2009-12-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.
Book Synopsis Handbook of Practical Immunohistochemistry by : Fan Lin
Download or read book Handbook of Practical Immunohistochemistry written by Fan Lin and published by Springer. This book was released on 2015-02-21 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
Book Synopsis Immunohistochemistry and Immunocytochemistry by : Simon Renshaw
Download or read book Immunohistochemistry and Immunocytochemistry written by Simon Renshaw and published by John Wiley & Sons. This book was released on 2017-02-15 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Book Synopsis Immunohistochemistry and Immunocytochemistry by : Luis Del Valle
Download or read book Immunohistochemistry and Immunocytochemistry written by Luis Del Valle and published by . This book was released on 2022 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field.
Book Synopsis Diagnostic Immunohistochemistry by : David J. Dabbs
Download or read book Diagnostic Immunohistochemistry written by David J. Dabbs and published by Churchill Livingstone. This book was released on 2006 with total page 858 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers current information and guidance on immunohistochemical diagnoses in surgical pathology and cytopathology. The book is organized in a consistent format of organ systems, specifically designed to target diagnostic dilemmas in surgical pathology. Each chapter features an introduction and a systematic approach to the diagnostic entities of that organ system. Provides a reference for pathologists practicing diagnostic surgical pathology and cytopathology. Structured in the organ-system approach to tumor pathology, with each chapter capable of standing alone. Extensive references and full-color illustrations are included.
Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Book Synopsis Basic Methods in Microscopy by : David L. Spector
Download or read book Basic Methods in Microscopy written by David L. Spector and published by CSHL Press. This book was released on 2006 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.
Book Synopsis Cytology E-Book by : Edmund S. Cibas
Download or read book Cytology E-Book written by Edmund S. Cibas and published by Elsevier Health Sciences. This book was released on 2013-12-23 with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt: Take advantage of comprehensive yet concise coverage of nearly every type of cytology, including gynecologic, non-gynecologic, and FNA. Understand the clinical implications of your diagnoses and better equip yourself to make effective interpretations with clinical correlations emphasized throughout. Make the most informed reporting decisions through an in-depth analysis of common diagnostic pitfalls. Quickly find the answers you need with succinct, bulleted text, valuable clinical pearls, and useful summaries of key points. Gain real-life perspective on a complete range of cytologic findings with over 700 crystal-clear, full-color illustrations. Access expert knowledge and key insights into HPV testing; new billing and coding guidelines; patient management discussions; and much more, thanks to comprehensive updates throughout. See how to perform fine needle biopsy aspiration with a new 17-minute video available at Expert Consult. Narrated by pathologist Dr. Amy Ly, the video includes a patient interview, precautions, and demonstrations of techniques.
Book Synopsis Histological & Histochemical Methods by : John Alan Kiernan
Download or read book Histological & Histochemical Methods written by John Alan Kiernan and published by Pergamon. This book was released on 1990 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc
Book Synopsis Morphology Methods by : Ricardo V. Lloyd
Download or read book Morphology Methods written by Ricardo V. Lloyd and published by Springer Science & Business Media. This book was released on 2001-06-15 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.
Book Synopsis In Situ Molecular Pathology and Co-expression Analyses by : Gerard J. Nuovo
Download or read book In Situ Molecular Pathology and Co-expression Analyses written by Gerard J. Nuovo and published by Academic Press. This book was released on 2020-09-03 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry. Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents easy-to-follow experimental protocols with tricks of the trade Includes two new chapters: Recent improvements in immunohistochemistry and in situ hybridization, Quality control for immunohistochemistry and in situ hybridization: How to know if the color change is signal or background The second edition also includes a detailed test to help one learn the basics of histologic interpretation of tissues and a separate detailed test in how to differentiate signal from background Includes chapter-ending summaries of Key Points to Remember, bringing beginners up to speed with any seasoned veteran in the field Thoughtfully tackles the molecular basis if IHC and ISH, along with application of that knowledge to improving the techniques is significant
