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Download or read book Immobilized Biochemical and Affinity Chromatography written by and published by . This book was released on 1974 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : R. Dunlap
Publisher : Springer
ISBN 13 : 9781468469844
Total Pages : 0 pages
Book Rating : 4.4/5 (698 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography written by R. Dunlap and published by Springer. This book was released on 2012-06-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains most of the papers presented at the Sym posium on Affinity Chromatography and Immobilized Biochemicals, which was held on November 7-9, 1973 in Charleston, South Carolina in conjunction with the Southeastern Regional American Chemical Society meeting. The topics of the symposium represent two new biochemical frontiers which have emerged in recent years through the ingenious development and application of solid phase biochemi cal technologies. Affinity chromatography involves the use of selected ligands, covalently bound to a solid support such as cel lulose, glass, Sepharose, or polyacrylamide and exploits the princi ple of biochemical recognition between the ligand in the solid phase and a selected macromolecule to facilitate the rapid and often quantitative purification of enzymes, antibodies, antigens, hormones, other proteins, etc. The area of immobilized biochemicals includes the use of coenzymes, oligo- and po1ynuc1eotides, enzymes, and multistep enzyme systems which are immobilized or entrapped in the solid phase. The goals of the symposium were the revi~w of the status of affinity chromatography and immobilized biochemicals, the presentation of new data and ideas in both areas, and the establish ment of a dialogue between research workers in these two evolving disciplines which are so closely interrelated. The papers published in this volume provide the reader with reviews of several topics inherent in the solid phase biochemistry together with a series of timely manuscripts concerning new techniques and applications both in the use of affinity chromatography and in the investigation of immobilized biochemicals.
Author : R Dunlap
Publisher : Springer
ISBN 13 : 9781468469837
Total Pages : 392 pages
Book Rating : 4.4/5 (698 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography written by R Dunlap and published by Springer. This book was released on 2014-01-15 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Pascal Bailon
Publisher : Springer Science & Business Media
ISBN 13 : 1603272615
Total Pages : 233 pages
Book Rating : 4.6/5 (32 download)
Download or read book Affinity Chromatography written by Pascal Bailon and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Author : O. Hoffmann-Ostenhof
Publisher : Elsevier
ISBN 13 : 1483188361
Total Pages : 391 pages
Book Rating : 4.4/5 (831 download)
Download or read book Affinity Chromatography written by O. Hoffmann-Ostenhof and published by Elsevier. This book was released on 2016-01-22 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography is a collection of papers that presents the advancement in various areas of affinity chromatography. The title particularly covers the development in affinity chromatography in the context of biochemistry and immunochemistry. The text first details gel matrices, coupling methods, and charge transfer chromatography. Next, the selection tackles the affinity chromatography of enzymes and hydrophobic interaction chromatography. The text also discusses matrix-bound antigens and antibodies, along with matrix-bound lectins and affinity chromatography of cells. Part VI deals with affinity chromatography of hormone receptors, while part vii talks about affinity techniques in the isolation Of DNA, RNA, and polysomes. The book will be of great use to students, researchers, and practitioners of biochemistry.
Author : J. Turková
Publisher : Elsevier
ISBN 13 : 0080858635
Total Pages : 819 pages
Book Rating : 4.0/5 (88 download)
Download or read book Bioaffinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1993-09-14 with total page 819 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography. Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.
Author : Symposium on Affinity Chromatography and Immobilized Biochemicals, Charleston, S.C., 1973
Publisher :
ISBN 13 :
Total Pages : 377 pages
Book Rating : 4.:/5 (1 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography. Edited by R. Bruce Dunlap written by Symposium on Affinity Chromatography and Immobilized Biochemicals, Charleston, S.C., 1973 and published by . This book was released on 1974 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 :
Total Pages : 494 pages
Book Rating : 4.F/5 ( download)
Download or read book Immobilized Affinity Ligand Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 1992-08-27 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References
Author : Irwin Chaiken
Publisher : Elsevier
ISBN 13 : 0323146627
Total Pages : 542 pages
Book Rating : 4.3/5 (231 download)
Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Author : That T. Ngo
Publisher : Springer Science & Business Media
ISBN 13 : 1489918728
Total Pages : 560 pages
Book Rating : 4.4/5 (899 download)
Download or read book Molecular Interactions in Bioseparations written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Author : Toni Kline
Publisher : CRC Press
ISBN 13 : 1482285010
Total Pages : 1198 pages
Book Rating : 4.4/5 (822 download)
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Author : Jan-Christer Janson
Publisher : John Wiley & Sons
ISBN 13 : 9780471186267
Total Pages : 712 pages
Book Rating : 4.1/5 (862 download)
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Author : Herbert Schott
Publisher : CRC Press
ISBN 13 : 1000110583
Total Pages : 249 pages
Book Rating : 4.0/5 (1 download)
Download or read book Affinity Chromatography written by Herbert Schott and published by CRC Press. This book was released on 2020-11-25 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book informs the reader about the practical methods, possibilities, and limits of template chromatography. It shows the various techniques for immobilization of nucleic acids fragments, polynucleotides, and nucleic acids by which the desired separation of materials can be achieved.
Author : Catherine Charcosset
Publisher : Elsevier
ISBN 13 : 0444563377
Total Pages : 349 pages
Book Rating : 4.4/5 (445 download)
Download or read book Membrane Processes in Biotechnology and Pharmaceutics written by Catherine Charcosset and published by Elsevier. This book was released on 2012-01-25 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane processes are increasingly used in pharmaceutical and biochemical engineering and biotechnology for concentration and purification, synthesis of molecules and drug delivery systems, and support for biochemical reactions. This book provides a state-of-the art overview of the classical membrane processes used in pharmaceutical and biochemical engineering and biotechnology, such as ultrafiltration, microfiltration, virus filtration, membrane chromatography, membrane emulsification, liquid membranes and membrane bioreactors. It describes the general rules (principles, choice of configurations, membranes, parameters, etc.), recent developments (fouling control, increase permeate flux and selectivity, etc.), applications, and theoretical descriptions. Further, it presents emerging processes such as solvent resistant nanofiltration and membrane crystallization. Presents classical membrane processes such as ultrafiltration, microfiltration, virus filtration, membrane chromatography, membrane emulsification, liquid membranes and membrane bioreactors Presents emerging processes such as solvent resistant nanofiltration and membrane crystallization Gives a complete description of each technique (principles, membrane materials and devices, fouling control, and theoretical description) Contains numerous examples of applications Includes a uniform notation throughout the book enhancing the presentation and understanding of the content Includes extensive list of references
Author : Sameh Magdeldin
Publisher : BoD – Books on Demand
ISBN 13 : 9535103253
Total Pages : 360 pages
Book Rating : 4.5/5 (351 download)
Download or read book Affinity Chromatography written by Sameh Magdeldin and published by BoD – Books on Demand. This book was released on 2012-03-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers.
Author : Paul Cutler
Publisher : Springer Science & Business Media
ISBN 13 : 159259655X
Total Pages : 474 pages
Book Rating : 4.5/5 (925 download)
Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Author : David S. Hage
Publisher : CRC Press
ISBN 13 : 0824751981
Total Pages : 878 pages
Book Rating : 4.8/5 (247 download)
Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
