Fluorescent Dye Labels And Stains

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Fluorescent Dye Labels and Stains

Author : Tarso B. Ledur Kist
Publisher : John Wiley & Sons
ISBN 13 : 1119835151
Total Pages : 500 pages
Book Rating : 4.1/5 (198 download)

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Book Synopsis Fluorescent Dye Labels and Stains by : Tarso B. Ledur Kist

Download or read book Fluorescent Dye Labels and Stains written by Tarso B. Ledur Kist and published by John Wiley & Sons. This book was released on 2023-03-10 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Dye Labels and Stains The only comprehensive database of fluorophores and their physical and photochemical properties Fluorophores are chemical compounds that strongly absorb in the ultraviolet, visible, and/or near-infrared and with bright emission in these ranges. As a result, they are exceptionally valuable as dyes for various analytical processes, capable of labelling and staining particular targets for purposes of fluorescent imaging, sensitive detection, and quantification (exhibiting linear responses over very wide concentration ranges). These compounds are many and varied, and panoramic views of their options, physical properties and their reactions to light excitations can be critical to their successful integration into chemical analysis, pharmaceutical analysis, clinical analysis, microscopies, optical bioimaging, cancer imaging, real-time PCR, flow cytometry, multiplexing in proteomics, life sciences in general, and many other high-tech fields (material sciences, traceability, photovoltaics, quantum computing). Fluorescent Dye Labels and Stains incorporates a comprehensive database of such substances and their characteristics. It provides an introduction to basic theories and foundational terminology, in addition to both the molecular structures and photophysical properties of an enormous range of fluorophores. Assembled over the course of a distinguished career in biochemistry, this database presents valuable information that has never before been available in a single volume. Readers will also find: Molecular and photochemical information of over 700 fluorophores A database of parameters, including light excitation ranges, molar absorption coefficients, fluorescence quantum yields, molecular brightness, and many more Information derived from multiple disciplines, including microscopy, nanoscopy, biochemistry, and molecular biology Fluorescent Dye Labels and Stains is the essential reference for pharmaceutical and biomedical researchers and professionals, academics who study molecular biology or organic chemistry, and any professional whose work includes strong and photostable molecular absorptions and fluorescence.

Bioconjugate Techniques

Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 : 0080568726
Total Pages : 1233 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Bioconjugate Techniques by : Greg T. Hermanson

Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

The Molecular Probes Handbook

Author : Iain Johnson
Publisher :
ISBN 13 : 9780982927915
Total Pages : 1060 pages
Book Rating : 4.9/5 (279 download)

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Book Synopsis The Molecular Probes Handbook by : Iain Johnson

Download or read book The Molecular Probes Handbook written by Iain Johnson and published by . This book was released on 2010 with total page 1060 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most complete fluorescent labeling and detection reference available, The Molecular Probes HandbookA Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 technology solutions representing a wide range of biomolecular labeling and detection reagents. The significantly revised 11th Edition features extensive references, reorganized content, and new technical notes and product highlights.

Handbook of Fluorescent Dyes and Probes

Author : R. W. Sabnis
Publisher : John Wiley & Sons
ISBN 13 : 1118028694
Total Pages : 468 pages
Book Rating : 4.1/5 (18 download)

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Book Synopsis Handbook of Fluorescent Dyes and Probes by : R. W. Sabnis

Download or read book Handbook of Fluorescent Dyes and Probes written by R. W. Sabnis and published by John Wiley & Sons. This book was released on 2015-06-02 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: A COMPLETE, UP-TO-DATE RESOURCE OF INFORMATION ON MORE THAN 150 FLUORESCENT DYES AND PROBES Handbook of Fluorescent Dyes and Probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in various fields. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, color, material, physical and related allied fields. The ease of use of the handbook is further enhanced by various appendixes provided at the end of the book to conveniently and easily locate the dye as per the reader's need. This is the first book to give the CAS registry numbers, chemical structure, Chemical Abstract (CA) index name, all other chemical names, Merck Index number, chemical/dye class, molecular formula, molecular weight, physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient, and quantum yield of fluorescent dyes and probes, as well as to provide access to synthetic procedures (lab scale and industrial scale) of dyes and probes in a single source. This user-friendly handbook also features references on safety, toxicity and adverse effects of dyes and probes on humans, animals and the environment, including: acute/chronic toxicity aquatic toxicity carcinogenicity cytotoxicity ecotoxicity genotoxocity hematotoxicity hepatotoxicity immunotoxicity marine toxicity microbial toxicity mutagenicity nephrotoxicity neurotoxicity nucleic acid damage oral toxicity phototoxicity phytotoxicity reproductive toxicity skin toxicity Containing imaging/labeling applications, biological/medical applications and industrial applications, Handbook of Fluorescent Dyes and Probes is a convenient, vital resource for industrial and academic researchers, and a valuable desktop reference for medical professionals, lab supervisors, scientists, chemists, biologists, engineers, physicists, intellectual property professionals, students, and professors. Includes all fluorescent dyes & probes known to date and provides a complete, up-to-date library of information in one reference/handbook Includes more than 300 fluorescent dyes & probes organized alphabetically by the commonly used Chemical Name Provides access to synthesis procedures (lab scale and industrial scale) of fluorescent dyes & probes First book to provide references on safety, toxicity and adverse effects of fluorescent dyes and probes on humans, animals, and the environment User-friendly and convenient resource guide for chemical, biological, medical, and intellectual property professionals in a broad range of disciplines

Conn's Biological Stains

Author : Richard W. Horobin
Publisher : Taylor & Francis
ISBN 13 : 9781859960998
Total Pages : 555 pages
Book Rating : 4.9/5 (69 download)

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Book Synopsis Conn's Biological Stains by : Richard W. Horobin

Download or read book Conn's Biological Stains written by Richard W. Horobin and published by Taylor & Francis. This book was released on 2002 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Published on behalf of the Biological Stain Commission For 75 years Conn's Biological Stains has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie's ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application. This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications.

Fluorescence Spectroscopy

Author : Otto S. Wolfbeis
Publisher : Springer Science & Business Media
ISBN 13 : 3642773729
Total Pages : 316 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Fluorescence Spectroscopy by : Otto S. Wolfbeis

Download or read book Fluorescence Spectroscopy written by Otto S. Wolfbeis and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC) The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.

Comprehensive Biophysics

Author :
Publisher : Academic Press
ISBN 13 : 0080957188
Total Pages : 3533 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Comprehensive Biophysics by :

Download or read book Comprehensive Biophysics written by and published by Academic Press. This book was released on 2012-04-12 with total page 3533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource

Fluorescence Microscopy in Life Sciences

Author : Juan Carlos Stockert
Publisher : Bentham Science Publishers
ISBN 13 : 1681085186
Total Pages : 751 pages
Book Rating : 4.6/5 (81 download)

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Book Synopsis Fluorescence Microscopy in Life Sciences by : Juan Carlos Stockert

Download or read book Fluorescence Microscopy in Life Sciences written by Juan Carlos Stockert and published by Bentham Science Publishers. This book was released on 2017-12-15 with total page 751 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

Protein Arrays

Author : Eric Fung
Publisher : Springer Science & Business Media
ISBN 13 : 1592597599
Total Pages : 279 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Protein Arrays by : Eric Fung

Download or read book Protein Arrays written by Eric Fung and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.

Fluorescent and Luminescent Probes for Biological Activity

Author : W. T. Mason
Publisher : Elsevier
ISBN 13 : 0080531776
Total Pages : 697 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Fluorescent and Luminescent Probes for Biological Activity by : W. T. Mason

Download or read book Fluorescent and Luminescent Probes for Biological Activity written by W. T. Mason and published by Elsevier. This book was released on 1999-04-16 with total page 697 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy

Public Health and Toxicology Issues in Drug Research, Volume 2

Author : Rakesh Kumar Tekade
Publisher : Elsevier
ISBN 13 : 0443158436
Total Pages : 854 pages
Book Rating : 4.4/5 (431 download)

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Book Synopsis Public Health and Toxicology Issues in Drug Research, Volume 2 by : Rakesh Kumar Tekade

Download or read book Public Health and Toxicology Issues in Drug Research, Volume 2 written by Rakesh Kumar Tekade and published by Elsevier. This book was released on 2024-04-05 with total page 854 pages. Available in PDF, EPUB and Kindle. Book excerpt: Toxicodynamics in Drug Research, Volume 2: Public Health and Toxicology Issues examines the implications of public health issues and the impact of pharmaceuticals, chemical and food toxicants, dietary phytochemicals, and medical treatments on human health. Volume 2: Public Health and Toxicology Issues in Drug Research: Toxicity and Toxicodynamics covers topics on pharmacokinetics and toxicokinetics such as population pharmacokinetics/toxicokinetics, the design of toxicokinetic studies, and the use of toxicokinetic data in preclinical safety assessments. The book investigates the health effect caused by the bioaccumulation of pharmaceutical and personal care products and the impact of drug-induced toxicity on different systems of the body. It discusses the mechanistic pathways of food toxicants and illustrates the molecular mechanisms of the chemopreventive role of dietary phytochemicals. It also delves into the toxic effects of medical treatments and materials. Ethical, legal, societal, and professional issues in toxicology round off the coverage providing a valuable resource to interested in learning more about the health impact and public health issues related to the toxicity of pharmaceuticals, dietary supplements, personal care products, and medical treatments. Discusses the impact of pharmaceuticals, food, and chemical toxicants on human health Examines the toxic effects of medical treatments, clinical administrations, and materials Explores public health issues around drug safety and toxicology

Basic Confocal Microscopy

Author : Robert L. Price
Publisher : Springer Science & Business Media
ISBN 13 : 0387781757
Total Pages : 307 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Basic Confocal Microscopy by : Robert L. Price

Download or read book Basic Confocal Microscopy written by Robert L. Price and published by Springer Science & Business Media. This book was released on 2011-04-11 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.

Functional Protein Microarrays in Drug Discovery

Author : Paul F. Predki
Publisher : CRC Press
ISBN 13 : 1420009842
Total Pages : 482 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Functional Protein Microarrays in Drug Discovery by : Paul F. Predki

Download or read book Functional Protein Microarrays in Drug Discovery written by Paul F. Predki and published by CRC Press. This book was released on 2007-03-30 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: An ideal text for biotechnologists, protein chemists, and biochemists, Functional Protein Microarrays in Drug Discovery explores all aspects of functional protein microarrays, including basic principles, methods, and applications. The book discusses the generation of functional protein content and describes both standard and state-of-the-art fabrication methods. It reviews current and next generation approaches to assay detection and presents a wide range of applications, from biomolecular interaction discovery and characterization to immune response profiling. The book also addresses several fundamental computational issues, bioinformatics and data analysis.

The Protein Protocols Handbook

Author : John M. Walker
Publisher : Springer
ISBN 13 : 1592591698
Total Pages : 1146 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis The Protein Protocols Handbook by : John M. Walker

Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1146 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.

The Handbook

Author : Richard P. Haugland
Publisher :
ISBN 13 : 9780971063648
Total Pages : 1126 pages
Book Rating : 4.0/5 (636 download)

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Book Synopsis The Handbook by : Richard P. Haugland

Download or read book The Handbook written by Richard P. Haugland and published by . This book was released on 2005 with total page 1126 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Practical Flow Cytometry

Author : Howard M. Shapiro
Publisher : John Wiley & Sons
ISBN 13 : 0471434035
Total Pages : 736 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro

Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

Author :
Publisher : Academic Press
ISBN 13 : 0128095474
Total Pages : 616 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods by :

Download or read book Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods written by and published by Academic Press. This book was released on 2016-10-28 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion