Fluorescence Spectroscopy Imaging And Probes

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Fluorescence Spectroscopy, Imaging and Probes

Author : Ruud Kraayenhof
Publisher : Springer Science & Business Media
ISBN 13 : 3642560679
Total Pages : 401 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Fluorescence Spectroscopy, Imaging and Probes by : Ruud Kraayenhof

Download or read book Fluorescence Spectroscopy, Imaging and Probes written by Ruud Kraayenhof and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.

Topics in Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306470608
Total Pages : 502 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Topics in Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Fluorescence Lifetime Spectroscopy and Imaging

Author : Laura Marcu
Publisher : CRC Press
ISBN 13 : 1439861684
Total Pages : 554 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu

Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Handbook of Fluorescence Spectroscopy and Imaging

Author : Markus Sauer
Publisher : John Wiley & Sons
ISBN 13 : 3527633529
Total Pages : 425 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Handbook of Fluorescence Spectroscopy and Imaging by : Markus Sauer

Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Analytical Use of Fluorescent Probes in Oncology

Author : Elli Kohen
Publisher : Springer Science & Business Media
ISBN 13 : 146155845X
Total Pages : 442 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Analytical Use of Fluorescent Probes in Oncology by : Elli Kohen

Download or read book Analytical Use of Fluorescent Probes in Oncology written by Elli Kohen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence is a very powerful tool for work at the frontier of cell biology, photobiology and bioinstrumentation. The stated aim of the workshop was to highlight the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particulary in terms of the analytical use of fluorescent probes in oncology. In the organization of the workshop a multidisciplinary approach was selected. The purpose of the Advanced Research Workshop (ARW) was to bring together researchers in the various disciplines of tissue optics, imaging, microspectrofluorometry and state of the art probes, in order to explore the full benefits that can be derived in biomedicine through the convergence of these approaches. When applied to in vivo and in situ studies, fluorescence and related optical methods enable us to explore within tissues, cells and organelles photon effects previously understood only in solution photochemistry. Processes which can be studied at the molecular level by photophysics, photochemistry and physical chemistry can be evaluated in living tissue by fluorescence spectroscopy and imaging at the intracellular level in terms of structure and function. Thus, fluorescence adds a new dimension to cell biology and physiology. This approach is now supported by a full and versatile, rapidly growing armamentarium of new selective probes for organelles, enzymes, cations, cytoskeleton and metabolic control.

Fluorescence Methods and Applications

Author : Otto S. Wolfbeis
Publisher : Wiley-Blackwell
ISBN 13 :
Total Pages : 352 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Fluorescence Methods and Applications by : Otto S. Wolfbeis

Download or read book Fluorescence Methods and Applications written by Otto S. Wolfbeis and published by Wiley-Blackwell. This book was released on 2008-07 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume features papers on new spectroscopic methods and techniques, the development and application of fluorescent probes, and new techniques and applications of fluorescence imaging. Specific areas include the following: fluorescence lifetime, fluorescence (in vivo) imaging, time-resolved fluorescence, luminescence anisotropy, fluorescent (NMIR) labels, luminescent lanthanides, fluorescent sensors and probes, fluorescence microscopy, FRET, fluorescent nanoparticles and dots, high-throughput screening, fluorescent bioassays, luminescence-based DNA technologies, FISH and immunohistochemistry, luminescence on metal surfaces, fluorescent proteins, upconversion, multiphoton fluorescence, confocal techniques, near-field and far-field techniques, single photon counting, fluorescence correlation spectroscopy (FCS), and flow cytometry. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit www.nyas.org/membership/main.asp for more information about becoming a member.

Topics in Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher :
ISBN 13 :
Total Pages : 501 pages
Book Rating : 4.:/5 (756 download)

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Book Synopsis Topics in Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by . This book was released on 2002 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fluorescent and Luminescent Probes for Biological Activity

Author : W. T. Mason
Publisher : Elsevier
ISBN 13 : 0080531776
Total Pages : 647 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Fluorescent and Luminescent Probes for Biological Activity by : W. T. Mason

Download or read book Fluorescent and Luminescent Probes for Biological Activity written by W. T. Mason and published by Elsevier. This book was released on 1999-04-16 with total page 647 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy

Fluorescence Applications in Biotechnology and Life Sciences

Author : Ewa M. Goldys
Publisher : John Wiley & Sons
ISBN 13 : 0470083700
Total Pages : 398 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Fluorescence Applications in Biotechnology and Life Sciences by : Ewa M. Goldys

Download or read book Fluorescence Applications in Biotechnology and Life Sciences written by Ewa M. Goldys and published by John Wiley & Sons. This book was released on 2009-08-24 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.

Fluorescence Spectroscopy and Microscopy

Author : Yves Engelborghs
Publisher : Humana
ISBN 13 : 9781493963157
Total Pages : 0 pages
Book Rating : 4.9/5 (631 download)

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Book Synopsis Fluorescence Spectroscopy and Microscopy by : Yves Engelborghs

Download or read book Fluorescence Spectroscopy and Microscopy written by Yves Engelborghs and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Advanced Concepts in Fluorescence Sensing

Author : Chris D. Geddes
Publisher : Springer Science & Business Media
ISBN 13 : 9780387233345
Total Pages : 346 pages
Book Rating : 4.2/5 (333 download)

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Book Synopsis Advanced Concepts in Fluorescence Sensing by : Chris D. Geddes

Download or read book Advanced Concepts in Fluorescence Sensing written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2005-06-28 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Advanced Fluorescence Reporters in Chemistry and Biology III

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
ISBN 13 : 3642180353
Total Pages : 352 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Advanced Fluorescence Reporters in Chemistry and Biology III by : Alexander P. Demchenko

Download or read book Advanced Fluorescence Reporters in Chemistry and Biology III written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2011-03-29 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.

Fluorescence Probes in Oncology

Author : Elli Kohen
Publisher : World Scientific
ISBN 13 : 178326215X
Total Pages : 736 pages
Book Rating : 4.7/5 (832 download)

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Book Synopsis Fluorescence Probes in Oncology by : Elli Kohen

Download or read book Fluorescence Probes in Oncology written by Elli Kohen and published by World Scientific. This book was released on 2002-05-20 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book. Contents:The Nature of LightIntroduction to PhotophysicsBasic Approaches to the Experimental Study of Chemical and Biological LuminescenceMethods and InstrumentationNew Methods. Theoretical Basis and Potential Applications to BiologyFluorescent ProbesApplications of Fluorescence Techniques to Study Biological Processes in Normal and Pathological CellsCell Physiopathology Readership: Students and researchers in oncology, the life sciences, biology and optics. Keywords:Chemiluminescence;Bioluminescence;Green Fluorescent Protein (GFP);Microspectrofluorimetry;Fluorescence Resonance Energy Transfer (FRET);Fluorescence Lifetime Imaging (FLIM);Fluorescence In Situ Hybridization (FISH);Multicolor Spectral Karyotyping (SKY);Fourier Interferometric Spectral Imaging;Fluorescence Imaging;Fluorescence Probe;Oncology;Cell Biology;Pathology;Diagnostics

Fluorescence Imaging for Surgeons

Author : Fernando D. Dip
Publisher : Springer
ISBN 13 : 3319156780
Total Pages : 360 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Fluorescence Imaging for Surgeons by : Fernando D. Dip

Download or read book Fluorescence Imaging for Surgeons written by Fernando D. Dip and published by Springer. This book was released on 2015-07-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text presents the experiences of leading researchers and surgeons with different fluorescence methods. Chapters range from basic science of fluorescence to current clinical applications and new horizons. The first few chapters describe the historical evolution and physical principles of fluorescence and provide the foundation for the reader to understand the current scope and limits of its use in surgery. The second section focuses on the clinical applications of intraoperative fluorescence imaging including subsections on fluorescence cholangiography, applications to hepatectomy, lymph node navigation, applications to GI tract and pelvic surgery and identification of cancer tissues. The third section focuses on new frontiers including fluorescence probes, imaging systems and applications to photodynamic therapy. Authored by leaders in the development of fluorescent methods worldwide, Fluorescence Imaging for Surgeons: Concepts and Applications will have an impact on numerous medical specialists including general surgeons, colorectal and minimally-invasive surgeons and surgical oncologists. Researchers will find the book to be an invaluable resource on the latest advances in the utilization of nanoparticles and fluorescent probes.

Fluorescence Spectroscopy in Biology

Author : Martin Hof
Publisher : Springer Science & Business Media
ISBN 13 : 3540270043
Total Pages : 315 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof

Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2006-01-27 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Techniques

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306438747
Total Pages : 462 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Techniques by : Joseph R. Lakowicz

Download or read book Techniques written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 1992-01-31 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this inaugural volume of a new series, experts in the field help biochemists, analytical chemists, spectroscopists, biophysicists, and other specialists keep up with the latest techniques and technologies available in fluorescence spectroscopy.

Topics in Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306470705
Total Pages : 544 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Topics in Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Topics in Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970’s. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980’s. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one’s self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.