Fluorescence Methods And Applications

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Fluorescence Methods and Applications

Author : Otto S. Wolfbeis
Publisher : Wiley-Blackwell
ISBN 13 :
Total Pages : 352 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Fluorescence Methods and Applications by : Otto S. Wolfbeis

Download or read book Fluorescence Methods and Applications written by Otto S. Wolfbeis and published by Wiley-Blackwell. This book was released on 2008-07 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume features papers on new spectroscopic methods and techniques, the development and application of fluorescent probes, and new techniques and applications of fluorescence imaging. Specific areas include the following: fluorescence lifetime, fluorescence (in vivo) imaging, time-resolved fluorescence, luminescence anisotropy, fluorescent (NMIR) labels, luminescent lanthanides, fluorescent sensors and probes, fluorescence microscopy, FRET, fluorescent nanoparticles and dots, high-throughput screening, fluorescent bioassays, luminescence-based DNA technologies, FISH and immunohistochemistry, luminescence on metal surfaces, fluorescent proteins, upconversion, multiphoton fluorescence, confocal techniques, near-field and far-field techniques, single photon counting, fluorescence correlation spectroscopy (FCS), and flow cytometry. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit www.nyas.org/membership/main.asp for more information about becoming a member.

Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

Author : David J. Suggett
Publisher : Springer Science & Business Media
ISBN 13 : 9048192684
Total Pages : 332 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications by : David J. Suggett

Download or read book Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications written by David J. Suggett and published by Springer Science & Business Media. This book was released on 2010-08-23 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.

Fluorescence Spectroscopy in Biology

Author : Martin Hof
Publisher : Springer Science & Business Media
ISBN 13 : 9783540223382
Total Pages : 330 pages
Book Rating : 4.2/5 (233 download)

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Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof

Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2004-12-08 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Fluorescence Microscopy

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
ISBN 13 : 3527687726
Total Pages : 496 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck

Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-03-27 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Lifetime Spectroscopy and Imaging

Author : Laura Marcu
Publisher : CRC Press
ISBN 13 : 1439861684
Total Pages : 554 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu

Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Molecular Fluorescence

Author : Bernard Valeur
Publisher : John Wiley & Sons
ISBN 13 : 3527650024
Total Pages : 595 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Molecular Fluorescence by : Bernard Valeur

Download or read book Molecular Fluorescence written by Bernard Valeur and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

New Trends in Fluorescence Spectroscopy

Author : Bernard Valeur
Publisher : Springer Science & Business Media
ISBN 13 : 364256853X
Total Pages : 496 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis New Trends in Fluorescence Spectroscopy by : Bernard Valeur

Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Fluorescence Applications in Biotechnology and Life Sciences

Author : Ewa M. Goldys
Publisher : John Wiley & Sons
ISBN 13 : 0470083700
Total Pages : 398 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Fluorescence Applications in Biotechnology and Life Sciences by : Ewa M. Goldys

Download or read book Fluorescence Applications in Biotechnology and Life Sciences written by Ewa M. Goldys and published by John Wiley & Sons. This book was released on 2009-08-24 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.

Fluorescence Spectroscopy in Biology

Author : Martin Hof
Publisher : Springer Science & Business Media
ISBN 13 : 3540270043
Total Pages : 315 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof

Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2006-01-27 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

X-Ray Fluorescence Spectroscopy for Laboratory Applications

Author : Michael Haschke
Publisher : John Wiley & Sons
ISBN 13 : 3527344632
Total Pages : 496 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis X-Ray Fluorescence Spectroscopy for Laboratory Applications by : Michael Haschke

Download or read book X-Ray Fluorescence Spectroscopy for Laboratory Applications written by Michael Haschke and published by John Wiley & Sons. This book was released on 2021-04-05 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides comprehensive coverage on using X-ray fluorescence for laboratory applications This book focuses on the practical aspects of X-ray fluorescence (XRF) spectroscopy and discusses the requirements for a successful sample analysis, such as sample preparation, measurement techniques and calibration, as well as the quality of the analysis results. X-Ray Fluorescence Spectroscopy for Laboratory Applications begins with a short overview of the physical fundamentals of the generation of X-rays and their interaction with the sample material, followed by a presentation of the different methods of sample preparation in dependence on the quality of the source material and the objective of the measurement. After a short description of the different available equipment types and their respective performance, the book provides in-depth information on the choice of the optimal measurement conditions and the processing of the measurement results. It covers instrument types for XRF; acquisition and evaluation of X-Ray spectra; analytical errors; analysis of homogeneous materials, powders, and liquids; special applications of XRF; process control and automation. An important resource for the analytical chemist, providing concrete guidelines and support for everyday analyses Focuses on daily laboratory work with commercially available devices Offers a unique compilation of knowledge and best practices from equipment manufacturers and users Covers the entire work process: sample preparation, the actual measurement, data processing, assessment of uncertainty, and accuracy of the obtained results X-Ray Fluorescence Spectroscopy for Laboratory Applications appeals to analytical chemists, analytical laboratories, materials scientists, environmental chemists, chemical engineers, biotechnologists, and pharma engineers.

Introduction to Fluorescence In Situ Hybridization

Author : Michael Andreeff
Publisher : Wiley-Liss
ISBN 13 : 9780471013457
Total Pages : 0 pages
Book Rating : 4.0/5 (134 download)

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Book Synopsis Introduction to Fluorescence In Situ Hybridization by : Michael Andreeff

Download or read book Introduction to Fluorescence In Situ Hybridization written by Michael Andreeff and published by Wiley-Liss. This book was released on 1999-12-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D., and Daniel Pinkel, PH.D. Fluorescence In Situ Hybridization (FISH) has become an essential tool in the diagnosis and management of a variety of solid tumors and hematologic malignancies in the clinical setting, as well as an aid in the identification of particular genetic disorders. Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH and covers in detail the applications of this technology to cancer in humans, including tumorigenesis, prostate and breast tumors, myeloid leukemias, and lymphoproliferative malignancies. The final section of the book is devoted to the applications of FISH techniques to genetic analysis. This incorporates radiation biodosimetry in chromosomal aberrations, genetic toxicology, and multiprobe methods for detecting chromosomally defective sperm in humans and mice, plus rapid prenatal diagnosis of chromosomal aneuploidies and detection of fetal trisomy. Edited by two leading basic and clinical investigators in the field, with state-of-the-art contributions from expert subspecialists, and featuring over 200 color illustrations, Introduction to Fluorescence In Situ Hybridization is an essential reference for clinicians and investigators in oncology, genetics, and cytometry.

Practical Fluorescence Spectroscopy

Author : Zygmunt (Karol) Gryczynski
Publisher : CRC Press
ISBN 13 : 1498752950
Total Pages : 804 pages
Book Rating : 4.4/5 (987 download)

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Book Synopsis Practical Fluorescence Spectroscopy by : Zygmunt (Karol) Gryczynski

Download or read book Practical Fluorescence Spectroscopy written by Zygmunt (Karol) Gryczynski and published by CRC Press. This book was released on 2019-12-18 with total page 804 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Fluorescence Spectroscopy and Microscopy

Author : Yves Engelborghs
Publisher : Humana
ISBN 13 : 9781493963157
Total Pages : 0 pages
Book Rating : 4.9/5 (631 download)

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Book Synopsis Fluorescence Spectroscopy and Microscopy by : Yves Engelborghs

Download or read book Fluorescence Spectroscopy and Microscopy written by Yves Engelborghs and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Fluorescence Spectroscopy

Author : Otto S. Wolfbeis
Publisher : Springer Science & Business Media
ISBN 13 : 3642773729
Total Pages : 316 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Fluorescence Spectroscopy by : Otto S. Wolfbeis

Download or read book Fluorescence Spectroscopy written by Otto S. Wolfbeis and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC) The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.

Advanced Concepts in Fluorescence Sensing

Author : Chris D. Geddes
Publisher : Springer Science & Business Media
ISBN 13 : 0387233350
Total Pages : 333 pages
Book Rating : 4.3/5 (872 download)

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Book Synopsis Advanced Concepts in Fluorescence Sensing by : Chris D. Geddes

Download or read book Advanced Concepts in Fluorescence Sensing written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2007-05-24 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Advanced Fluorescence Reporters in Chemistry and Biology III

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
ISBN 13 : 3642180353
Total Pages : 352 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Advanced Fluorescence Reporters in Chemistry and Biology III by : Alexander P. Demchenko

Download or read book Advanced Fluorescence Reporters in Chemistry and Biology III written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2011-03-29 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.

Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 146157658X
Total Pages : 487 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.