Author : Alexandria E. Kesterson
Publisher :
ISBN 13 : 9781321905915
Total Pages : 90 pages
Book Rating : 4.9/5 (59 download)
Book Synopsis Evaluation of the Role of Cell-mediated Immunity in Efficacy of Experimental Alternative Schedule of Live Attenuated RB51 Vaccine Against Brucellosis in Cattle by : Alexandria E. Kesterson
Download or read book Evaluation of the Role of Cell-mediated Immunity in Efficacy of Experimental Alternative Schedule of Live Attenuated RB51 Vaccine Against Brucellosis in Cattle written by Alexandria E. Kesterson and published by . This book was released on 2015 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: The hypothesis of this study is that RB51 booster vaccinations will provide either increased cell mediated or humoral immunity as compared to a single calf-hood vaccination alone. This research includes four specific aims; 1) that multiple doses of RB51 vaccine will induce a measurable increase in cell-mediated and humoral immunity and that this will correlate with a decreased incidence of infection and abortion at challenge; 2) that multiple doses of RB51 will not induce abortion when an adult booster vaccination is given during pregnancy; 3) that we will be able to correlate cell-mediated immunity data with a predicted outcome at challenge to establish an immune correlate for vaccination; 4) that RB51 titers to multiple doses of vaccine will correlate with previous studies in terms of safety of the vaccine. Cattle have been divided into four treatment groups; three treatment groups (n=7 each) and a control group (n=4). Each treatment group was assigned to be vaccinated with zero, one, two, or three doses of vaccine. All cattle were treated under normal ranching conditions and were vaccinated for diseases following the usual protocols for this region. Cell-mediated immunity has been measured through semi-quantitative PCR, cytokine suspension array, flow cytometry, and cell proliferation and killing assays. Humoral immunity was measured through an RB51 ELISA developed de novo at the Wyoming State Veterinary Laboratory (WSVL). Cell killing assays were used to determine the up-regulation in cytokine expression and cell killing. An immortalized bovine macrophage cell line was obtained from the United States Department of Agriculture facility in Ames, IA to perform cell-mediated immunity assays. Baseline results will be presented for all assays listed above. The results will reflect data from all three RB51 vaccine doses. Conclusions will be presented as the characterization and comparison of all three vaccine associated immune responses. Vaccinated cattle and control cattle were challenged with B. abortus S2308 to determine the efficacy of multiple doses on bacterial burden and incidence of abortion.