Enzyme Linked Immunosorbent Assay Elisa

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Enzyme-linked Immunosorbent Assay (ELISA)

Author : Samira Hosseini
Publisher : Springer
ISBN 13 : 981106766X
Total Pages : 124 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Enzyme-linked Immunosorbent Assay (ELISA) by : Samira Hosseini

Download or read book Enzyme-linked Immunosorbent Assay (ELISA) written by Samira Hosseini and published by Springer. This book was released on 2017-12-30 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

Basic Science Methods for Clinical Researchers

Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 383 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

ELISA

Author : Robert Hnasko
Publisher : Humana
ISBN 13 : 9781493927418
Total Pages : 0 pages
Book Rating : 4.9/5 (274 download)

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Book Synopsis ELISA by : Robert Hnasko

Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.

Techniques in Molecular Biology

Author : J.M. Walker
Publisher : Springer Science & Business Media
ISBN 13 : 9401165637
Total Pages : 304 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Techniques in Molecular Biology by : J.M. Walker

Download or read book Techniques in Molecular Biology written by J.M. Walker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

The ELISA Guidebook

Author : John R. Crowther
Publisher : Humana
ISBN 13 : 9781617378843
Total Pages : 0 pages
Book Rating : 4.3/5 (788 download)

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Book Synopsis The ELISA Guidebook by : John R. Crowther

Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.

ELISA

Author : John R. Crowther
Publisher : Springer Science & Business Media
ISBN 13 : 1592595294
Total Pages : 231 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis ELISA by : John R. Crowther

Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

Biosensors Based on Sandwich Assays

Author : Fan Xia
Publisher : Springer
ISBN 13 : 9811078351
Total Pages : 220 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Biosensors Based on Sandwich Assays by : Fan Xia

Download or read book Biosensors Based on Sandwich Assays written by Fan Xia and published by Springer. This book was released on 2018-01-09 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.

Basic Serological Testing

Author : Rowa Yousef Alhabbab
Publisher : Springer
ISBN 13 : 3319776940
Total Pages : 144 pages
Book Rating : 4.3/5 (197 download)

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Book Synopsis Basic Serological Testing by : Rowa Yousef Alhabbab

Download or read book Basic Serological Testing written by Rowa Yousef Alhabbab and published by Springer. This book was released on 2018-05-23 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing.

Techniques to Measure Food Safety and Quality

Author : Mohidus Samad Khan
Publisher : Springer Nature
ISBN 13 : 3030686361
Total Pages : 478 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis Techniques to Measure Food Safety and Quality by : Mohidus Samad Khan

Download or read book Techniques to Measure Food Safety and Quality written by Mohidus Samad Khan and published by Springer Nature. This book was released on 2021-09-22 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the basic understanding of food contaminants and their sources, followed by the techniques to measure food safety and quality. It is divided into four parts: Part A - sources of contaminants in foods, their associated health risks, and integrated management and alternative options to minimize contaminants; Part B - Technological assessment of conventional methods and selected advanced methods for the detection, identification and enumeration of microbial contaminates; Part C - Technological assessment of different chemical measurements techniques; and Part D – Technological assessment of different instrumental techniques to assess sensory properties of foods. Food safety is a growing concern due to the increase in food-borne illnesses caused by food adulteration, excessive use of pesticides, use of chemical preservatives and artificial fruit ripening agents, microbial contaminations, and improper food handling. Chemical contaminants in food could be transferred from environmental or agrochemical sources, personal care products, and other by-products of water disinfects. In addition, microbial food safety can be threatened due to the presence of many pathogens, such as Salmonella, Escherichia coli, Clostridium botulinum, Staphylococcus aureus, and Listeria monocytogenes in foods. Globally, strict regulations are imposed to limit the potential contaminants in foods. Development of accurate, rapid, and inexpensive approaches to test food contamination and adulteration would be highly valued to ensure global food safety. There are existing processes to ensure safety of food products from chemical and microbial contaminants. Apart from the existing measurement technologies, varieties of new techniques are also being emerged and these could be potential to ensure food safety and quality. In addition to chemical and microbial properties, sensory properties such as texture, mouth feel, flavor, and taste, are among the most important attributes of food products to ensure their acceptability by consumers. Two approaches are available to evaluate sensory properties of food products, namely subjective and objective analyses. The responses are perceived by all five senses: smell, taste, sight, touch, and hearing. The approach used in sensory evaluation varies depending on the types of foods and the ultimate goal of the testing. Sensory attributes are the most important quality parameters after ensuring the safety of foods.

Immunology: Overview and Laboratory Manual

Author : Tobili Y. Sam-Yellowe
Publisher : Springer
ISBN 13 : 9783030646882
Total Pages : 0 pages
Book Rating : 4.6/5 (468 download)

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Book Synopsis Immunology: Overview and Laboratory Manual by : Tobili Y. Sam-Yellowe

Download or read book Immunology: Overview and Laboratory Manual written by Tobili Y. Sam-Yellowe and published by Springer. This book was released on 2022-08-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin

Author : V.K. Agarwal
Publisher : Springer Science & Business Media
ISBN 13 : 1461533562
Total Pages : 260 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin by : V.K. Agarwal

Download or read book Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin written by V.K. Agarwal and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last three decades. use of antibiotics/drugs in animal husbandry programs has grown tremendously. Antibiotics/drugs are used therapeutically to cure diseases. and subtherapeutically to control the outbreak of diseases, improve feed efficiency and promote growth. The presence of antibiotic/drug residues in food products of animal origin. i.e •• meat, poultry and milk, can be a potential health hazard to consumers. Significant research is being done to develop new methods or to improve on existing methods to confirm and quantitatively determine the antibiotic/drug residues in meat, poultry and milk. This book covers recent development and application of various analytical techniques for the determination of antibiotic/drug residues in food products of animal origin. I thank the authors for their time and efforts in preparing the manuscripts and "all the reviewers for reviewing the manuscripts. I also thank to the Division of Agricultural and Food Chemistry of the American Chemical Society for sponsoring the symposium and Hewlett Packard, Palo Alto, CA, Perkin Elmer Corp., Norwalk, CT, Millipore Corporation, Milford, MA, and Kraft General Foods, Glenview, IL, for their generous financial support for the symposium. Vipin K. Agarwal New Haven, CT v CONTENTS Importance of Laboratory Validations and Accurate Descriptions of Analytical Procedures for Drug Residues in Foods ................. .

Experimental Biochemistry

Author : Robert L. Switzer
Publisher : Macmillan
ISBN 13 : 9780716733003
Total Pages : 468 pages
Book Rating : 4.7/5 (33 download)

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Book Synopsis Experimental Biochemistry by : Robert L. Switzer

Download or read book Experimental Biochemistry written by Robert L. Switzer and published by Macmillan. This book was released on 1999-04-21 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Biochemistry provides comprehensive coverage of important techniques used in contemporary biochemical research and gives students the background theory they need to understand the nature of the experiments.

Quantitative Enzyme Immunoassay

Author : Eva Engvall
Publisher : Blackwell Publishers
ISBN 13 :
Total Pages : 148 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis Quantitative Enzyme Immunoassay by : Eva Engvall

Download or read book Quantitative Enzyme Immunoassay written by Eva Engvall and published by Blackwell Publishers. This book was released on 1978 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Protein-Protein Interactions

Author : Weibo Cai
Publisher : BoD – Books on Demand
ISBN 13 : 9535103970
Total Pages : 488 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Protein-Protein Interactions by : Weibo Cai

Download or read book Protein-Protein Interactions written by Weibo Cai and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.

Elisa

Author : Rezaul Karim
Publisher :
ISBN 13 : 9781536143935
Total Pages : 0 pages
Book Rating : 4.1/5 (439 download)

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Book Synopsis Elisa by : Rezaul Karim

Download or read book Elisa written by Rezaul Karim and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: History, Types and Applications contains unique combinations of chapters in which the abstract of each chapter defines the work considered for the entire chapter. One of the essential goals of this book is to delineate vital information about different types of ELISA and their applications. The beginning chapter describes the history and background of enzyme-linked immunosorbent assay (ELISA), as well as the remarkable changes that were brought about by this technique. The chapter also describes the commercial application techniques used in food and diagnostic industries. The second chapter, on the other hand, describes various types of ELISA and their steps to perform ELISA. The third chapter discusses the practical applications and their mechanisms from theory. It also discusses possible multiple sources of error which can exist at each stage of the process; how to minimize the error; and the principles of data analysis. The fourth chapter discusses the applications of ELISA in various fields, which also include the uses of ELISA in the diagnosis of infectious diseases, parasitic diseases, and non-communicable disease caused by a variety of pathogenic microorganisms. While Chapters One to Four describe a detailed history, a variety of ELISA, their steps, their sources of application error, their solutions and their applications in different fields, the remaining chapters discuss more specific issues, including: The role of ELISA in advanced glycerin end products and their cell surface receptors in metabolic diseases, indirect ELISA for the diagnosis of Brucella Canis, ELISA based detection in cancer research, etc.

A Practical Guide to ELISA

Author : D. M. Kemeny
Publisher : Pergamon
ISBN 13 :
Total Pages : 132 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis A Practical Guide to ELISA by : D. M. Kemeny

Download or read book A Practical Guide to ELISA written by D. M. Kemeny and published by Pergamon. This book was released on 1991 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.

Plant Cell, Tissue and Organ Culture

Author : Oluf Gamborg
Publisher : Springer Science & Business Media
ISBN 13 : 3642790488
Total Pages : 367 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Cell, Tissue and Organ Culture by : Oluf Gamborg

Download or read book Plant Cell, Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.