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Enhancing Protein Secretion In Escherichia Coli By Codon Engineering Via Translation Optimization And Genome Sequence Analysis Of A Hypersecreter Mutant
Download Enhancing Protein Secretion In Escherichia Coli By Codon Engineering Via Translation Optimization And Genome Sequence Analysis Of A Hypersecreter Mutant full books in PDF, epub, and Kindle. Read online Enhancing Protein Secretion In Escherichia Coli By Codon Engineering Via Translation Optimization And Genome Sequence Analysis Of A Hypersecreter Mutant ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Enhancing Protein Secretion in Escherichia Coli by Codon Engineering Via Translation Optimization and Genome Sequence Analysis of a Hypersecreter Mutant by : Prateek Gupta
Download or read book Enhancing Protein Secretion in Escherichia Coli by Codon Engineering Via Translation Optimization and Genome Sequence Analysis of a Hypersecreter Mutant written by Prateek Gupta and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Effect of Codon Optimization on Bacterial Translation Elongation Rates by : Clay Swackhamer
Download or read book Effect of Codon Optimization on Bacterial Translation Elongation Rates written by Clay Swackhamer and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Microorganisms are used in a variety of industries to produce bioproducts, including fuels, food products, specialty chemicals, and even lucrative biologic therapeutics. These products are created through manipulation of the natural ability of microorganisms to use genetic information to produce proteins. This is known as the central dogma of biology, and is mediated by the steps of DNA being transcribed to mRNA and then translated into protein. It is the task of engineers to use an understanding of these component biophysical processes to control protein synthesis through genetic level controls, and to create new systems for protein production using the principles of rational design. Using engineering knowledge to raise protein expression is a goal in numerous industries, as higher protein expression is a driver of overall profit.There are several genetic level points of control currently used by engineers to raise protein expression, one of which is codon optimization of the coding sequences used by microorganisms to produce proteins. Since there are 20 amino acids but 64 codons, there are instances were multiple codons are translated into the same amino acid. These are called degenerate codons. However, degenerate codons may not have the same translational efficiency. Traditional codon optimization methods in E. coli rely on the preference for certain codons across the entire genome, yet this is not the only possible approach. The principal challenge is that at high translation initiation rates, protein expression may plateau, and it is hypothesized that novel criteria for codon optimization of genes can be used to raise expression plateaus that occur when translation elongation becomes the rate limiting step in protein synthesis, and also allow for fine tuning of expression due to predicted differences in translational efficiency between degenerate codons. In this research, novel criteria for codon optimization were employed to design and create synthetic variants of a reporter gene that was then characterized in vivo using an expression construct. Fluorescence levels of cells expressing these constructs were measured and results suggest that protein expression plateaus may still be experienced, even by the sequences optimized for high efficiency. However, the new criteria for codon optimization, for example the statistical correlation between a degenerate codon and its presence in highly translated parts of the genome, are feasible for use in future projects. This may enable future researchers to optimize genes at the codon level with greater fidelity.
Book Synopsis Direct Microbial Conversion of Biomass to Advanced Biofuels by : Michael E Himmel
Download or read book Direct Microbial Conversion of Biomass to Advanced Biofuels written by Michael E Himmel and published by Elsevier. This book was released on 2015-05-19 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'Direct Microbial Conversion of Biomass to Advanced Biofuels' is a stylized text that is rich in both the basic and applied sciences. It provides a higher level summary of the most important aspects of the topic, addressing critical problems solved by deep science. Expert users will find new, critical methods that can be applied to their work, detailed experimental plans, important outcomes given for illustrative problems, and conclusions drawn for specific studies that address broad based issues. A broad range of readers will find this to be a comprehensive, informational text on the subject matter, including experimentalists and even CEOs deciding on new business directions. Describes an important new field in biotechnology, the consolidated conversion of lignocellulosic feedstocks to advanced fuels Up-to-date views of promising technologies used in the production of advanced biofuels Presents the newest ideas, well-designed experiments, and outcomes Provides outstanding illustrations from NREL and contributing researchers Contains contributions from leaders in the field that provide numerous examples and insights into the most important aspects of the topic
Book Synopsis Biotechnology for Biofuel Production and Optimization by : Carrie A Eckert
Download or read book Biotechnology for Biofuel Production and Optimization written by Carrie A Eckert and published by Elsevier. This book was released on 2016-01-19 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology for Biofuel Production and Optimization is the compilation of current research findings that cover the entire process of biofuels production from manipulation of genes and pathways to organisms and renewable feedstocks for efficient biofuel production as well as different cultivation techniques and process scale-up considerations. This book captures recent breakthroughs in the interdisciplinary areas of systems and synthetic biology, metabolic engineering, and bioprocess engineering for renewable, cleaner sources of energy. Describes state-of-the-art engineering of metabolic pathways for the production of a variety of fuel molecules Discusses recent advances in synthetic biology and metabolic engineering for rational design, construction, evaluation of novel pathways and cell chassis Covers genome engineering technologies to address complex biofuel-tolerant phenotypes for enhanced biofuel production in engineered chassis Presents the use of novel microorganisms and expanded substrate utilization strategies for production of targeted fuel molecules Explores biohybrid methods for harvesting bioenergy Discusses bioreactor design and optimization of scale-up
Book Synopsis Mycobacterium ulcerans by : Gerd Pluschke
Download or read book Mycobacterium ulcerans written by Gerd Pluschke and published by Humana. This book was released on 2022-10-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.
Book Synopsis Advances in 2nd Generation of Bioethanol Production by : Xin Lu
Download or read book Advances in 2nd Generation of Bioethanol Production written by Xin Lu and published by Woodhead Publishing. This book was released on 2021-01-29 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in 2nd Generation of Bioethanol Production presents a comprehensive overview of technologies and strategies for the conversion of lignocellulosic biomass. This includes issues like sustainable production, environmental and economic benefits, and the main hurdles for upscaling and achieving commercial viability. The book assesses the current biomass conversion technologies, their readiness level for commercial production, and applications of bioethanol in bioenergy and chemical feedstock. The essential conversion process of 2nd generation biofuels, including feedstock composition and pretreatment, is then broken down, with special focus on advantages and pitfalls of each feedstock and process. It also explores the advances and challenges of bioprocessing, hydrolysis technologies and simultaneous fermentation of pentose and hexose. Finally, it presents the current status and bottlenecks for industrial production of bioethanol, as well as its future prospects. Its interdisciplinary approach, drawing upon plant biology, chemistry, biochemistry, microbiology, and genetics, makes Advances in 2nd Generation of Bioethanol Production a must-have reference for researchers in academia and industry R&D. It allows them to compare challenges and opportunities of new technologies and identify the gaps where new technology is needed. Practitioners in the industry also benefit from the information on working principles, design and control of the bioethanol production process, highlighting areas where technology innovation and investment should be placed. Graduate students and researchers newly entered in this field find here a key-resource to thoroughly understand the process as well as the fundamentals of bioethanol and bioproducts production from lignocellulosic biomass. Presents fundamentals and state-of-the-art of available pathways for bioethanol and bioproducts production from lignocellulosic biomass Discusses key-challenges for large scale production of bioethanol, such as pretreatment and hydrolysis Covers the specificities of various feedstocks and processes, the role of microorganisms in fermentation, saccharification limitations and challenges in the C5 and C6 fermentation
Book Synopsis Bioprocessing for Value-Added Products from Renewable Resources by : Shang-Tian Yang
Download or read book Bioprocessing for Value-Added Products from Renewable Resources written by Shang-Tian Yang and published by Elsevier. This book was released on 2011-08-11 with total page 685 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioprocessing for Value-Added Products from Renewable Resources provides a timely review of new and unconventional techniques for manufacturing high-value products based on simple biological material. The book discusses the principles underpinning modern industrial biotechnology and describes a unique collection of novel bioprocesses for a sustainable future. This book begins in a very structured way. It first looks at the modern technologies that form the basis for creating a bio-based industry before describing the various organisms that are suitable for bioprocessing - from bacteria to algae - as well as their unique characteristics. This is followed by a discussion of novel, experimental bioprocesses, such as the production of medicinal chemicals, the production of chiral compounds and the design of biofuel cells. The book concludes with examples where biological, renewable resources become an important feedstock for large-scale industrial production. This book is suitable for researchers, practitioners, students, and consultants in the bioprocess and biotechnology fields, and for others who are interested in biotechnology, engineering, industrial microbiology and chemical engineering. ·Reviews the principles underpinning modern industrial biotechnology ·Provides a unique collection of novel bioprocesses for a sustainable future ·Gives examples of economical use of renewable resources as feedstocks ·Suitable for both non-experts and experts in the bioproduct industry
Book Synopsis Biotransformation of Agricultural Waste and By-Products by : Palmiro Poltronieri
Download or read book Biotransformation of Agricultural Waste and By-Products written by Palmiro Poltronieri and published by Elsevier. This book was released on 2016-03-01 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotransformation of Agricultural Waste and By-Products in the 4F Economy: The Food, Feed, Fiber, Fuel (4F) Economy presents an evaluation of plant species better exploitable for a particular transformation. As crops are already covering large parts of cultivable soils, is it is not conceivable to try to extend the cultures beyond the limit of available soils, but a further increase in productivity is not easy to obtain. The book discusses advances in technology and plants design which support the exploitation and valorization of vegetable and fruit by-products through fermentation (feed-batch liquid fermentation, solid-state fermentation) in bio-based bio-chemicals/biofuels production. Pathways in the biosynthesis of fibers, sugars, and metabolites are provided with a focus on the lifecycle of bacteria, yeasts, and even plant species. The text analyzes cellular structures and the organization of cell walls in order to show which polysaccharides offer more favorable fermentative processes and which are detrimental. Provides an overview of all plant based biosources Includes examples of biochemical/biofuel production from plant waste Discusses the production of enzymes used in the plant fermentation processes Explores the new fermentation technologies and production of chemicals and fuels from various plants
Book Synopsis Advanced Bioprocessing for Alternative Fuels, Biobased Chemicals, and Bioproducts by : Majid Hosseini
Download or read book Advanced Bioprocessing for Alternative Fuels, Biobased Chemicals, and Bioproducts written by Majid Hosseini and published by Academic Press. This book was released on 2019-02-23 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Bioprocessing for Alternative Fuels, Bio-based Chemicals, and Bioproducts: Technologies and Approaches for Scale-Up and Commercialization demonstrates novel systems that apply advanced bioprocessing technologies to produce biofuels, bio-based chemicals, and value-added bioproducts from renewable sources. The book presents the use of novel oleaginous microorganisms and utilization strategies for applications of advanced bioprocessing technology in biofuels production and thoroughly depicts the technological breakthroughs of value added bioproducts. It also aides in the design, evaluation and production of biofuels by describing metabolic engineering and genetic manipulation of biofuels feedstocks. Users will find a thorough overview of the most recent discoveries in biofuels research and the inherent challenges associated with scale up. Emphasis is placed on technological milestones and breakthroughs in applications of new bioprocessing technologies for biofuels production. Its essential information can be used to understand how to incorporate advanced bioprocessing technologies into the scaling up of laboratory technologies to industrial applications while complying with biofuels policies and regulations. Presents the use of novel oleaginous microorganisms and utilization strategies for the applications of advanced technologies in biofuels production Provides a basis for technology assessments, progress and advances, as well as the challenges associated with biofuels at industrial scale Describes, in detail, technologies for metabolic engineering and genetic manipulation of biofuels feedstocks, thus aiding in the design, evaluation and production of advanced biofuels
Book Synopsis Advances in Sugarcane Biorefinery by : Anuj K. Chandel
Download or read book Advances in Sugarcane Biorefinery written by Anuj K. Chandel and published by Elsevier. This book was released on 2017-12-05 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Sugarcane Biorefinery: Technologies, Commercialization, Policy Issues and Paradigm Shift for Bioethanol and By-Products, by Chandel and Silveira, compiles the basic and applied information covering cane and biomass processing for sugar and ethanol production, as well as by-products utilization for improving the economy of sugarcane biorefineries. In this unique collection of 14 chapters, specialists in their field provide critical insights into several topics, review the current research, and discuss future progress in this research area. The book presents the most current advances in sugarcane biorefinery, including sugarcane crop cultivation, new sugarcane varieties, soil health, mechanization of crop, technical aspects of first and second generation ethanol production, economic analysis, life cycle assessment, biomass logistics and storage, co-generation of heat and electricity, process intensification and alternative by-products utilization. The book also explores the business ecosystem of sugarcane biorefineries, marketing analysis of ethanol demand and price dwindling patterns, aiming for a futuristic scenario. This book will be especially useful for scientists, researchers and technicians who are working in the area of biomass based biorefineries, as well as professionals in the sugar and alcohol industry. It also brings relevant content for policy makers, market analysts, agriculture scientists and managers. Presents technological updates on biomass processing, system biology, microbial fermentation, catalysis, regeneration and monitoring of renewable energy and recovery processes Includes topics on techno-economic analysis, life cycle assessment, sustainability, markets and policy Explores the future potential of biorefineries with zero or near zero waste, and the potential of valorization of all by-products, including alternatives to current applications and the management of a large amount of residues
