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Differential Display
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Author :Sergio Colonna-Romano Publisher :Springer Science & Business Media ISBN 13 :3642804543 Total Pages :135 pages Book Rating :4.6/5 (428 download)
Book Synopsis Differential-Display Reverse Transcription-PCR (DDRT-PCR) by : Sergio Colonna-Romano
Download or read book Differential-Display Reverse Transcription-PCR (DDRT-PCR) written by Sergio Colonna-Romano and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.
Book Synopsis Differential Display Methods and Protocols by : Peng Liang
Download or read book Differential Display Methods and Protocols written by Peng Liang and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.
Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Animal Genomics by : Sukanta Mondal
Download or read book Advances in Animal Genomics written by Sukanta Mondal and published by Academic Press. This book was released on 2020-11-25 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Animal Genomics provides an outstanding collection of integrated strategies involving traditional and modern - omics (structural, functional, comparative and epigenomics) approaches and genomics-assisted breeding methods which animal biotechnologists can utilize to dissect and decode the molecular and gene regulatory networks involved in the complex quantitative yield and stress tolerance traits in livestock. Written by international experts on animal genomics, this book explores the recent advances in high-throughput, next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches which have enabled to produce huge genomic and transcriptomic resources globally on a genome-wide scale. This book is an important resource for researchers, students, educators and professionals in agriculture, veterinary and biotechnology sciences that enables them to solve problems regarding sustainable development with the help of current innovative biotechnologies. - Integrates basic and advanced concepts of animal biotechnology and presents future developments - Describes current high-throughput next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches for sustainable livestock production - Illustrates integrated strategies to dissect and decode the molecular and gene regulatory networks involved in complex quantitative yield and stress tolerance traits in livestock - Ensures readers will gain a strong grasp of biotechnology for sustainable livestock production with its well-illustrated discussion
Book Synopsis Differentially Expressed Genes In Plants by : Axel Kornerup Hansen
Download or read book Differentially Expressed Genes In Plants written by Axel Kornerup Hansen and published by CRC Press. This book was released on 2002-09-11 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of changes in gene activity in tissues and cells of plants is a way of measuring developmental and environmental responses. This volume provides detailed accounts of new and established techniques used to carry out such analyses.
Book Synopsis Handbook of Neurochemistry and Molecular Neurobiology by : Glen Baker
Download or read book Handbook of Neurochemistry and Molecular Neurobiology written by Glen Baker and published by Springer Science & Business Media. This book was released on 2007-03-26 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook is intended to be a service to the neuroscience community, to help in finding available and useful information, to point out gaps in our knowledge, and to encourage continued studies. It represents the valuable contributions of the many authors of the chapters and the guidance of the editors and most important, it represents support for research in this discipline. Based on the rapid advances in the years since the second edition
Book Synopsis Tensors, Differential Forms, and Variational Principles by : David Lovelock
Download or read book Tensors, Differential Forms, and Variational Principles written by David Lovelock and published by Courier Corporation. This book was released on 2012-04-20 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Incisive, self-contained account of tensor analysis and the calculus of exterior differential forms, interaction between the concept of invariance and the calculus of variations. Emphasis is on analytical techniques. Includes problems.
Book Synopsis Differential Forms and Connections by : R. W. R. Darling
Download or read book Differential Forms and Connections written by R. W. R. Darling and published by Cambridge University Press. This book was released on 1994-09-22 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introducing the tools of modern differential geometry--exterior calculus, manifolds, vector bundles, connections--this textbook covers both classical surface theory, the modern theory of connections, and curvature. With no knowledge of topology assumed, the only prerequisites are multivariate calculus and linear algebra.
Book Synopsis Medical BioMethods Handbook by : John M. Walker
Download or read book Medical BioMethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2007-10-09 with total page 635 pages. Available in PDF, EPUB and Kindle. Book excerpt: John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
Book Synopsis Handbook of Molecular-Genetic Techniques for Brain and Behavior Research by : Wim E. Crusio
Download or read book Handbook of Molecular-Genetic Techniques for Brain and Behavior Research written by Wim E. Crusio and published by Elsevier. This book was released on 1999-10-18 with total page 994 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book gives a broad overview of recombinant DNA techniques for the behavioral neuroscientist, with illustrative examples of applications. Species covered include rodents (mainly mice), Drosophila melanogaster, Caenorhabditis elegans and Danio rerio. Experimental techniques required to characterize the behavioral phenotypes of mutant animals is provided. Several aspects of novel molecular-genetic techniques are overviewed and possible research strategies are explained. The sections of the book start with general descriptions of techniques followed by illustrative examples. It is divided into six sections. Section 1, bioinformatics and genomics research. Section 2, top-down strategies, where the researcher starts with the phenotype and then analyzes the associated genes; bottom-up strategies, where the physiological chain leading to a phenotype is analyzed starting from the gene product. Section 3, transgenic approaches in rodents including overexpressing foreign genes and gene-targeting; systemic manipulation approaches directly targeting the central nervous system and methods used with invertebrates. Section 4, methods used to evaluate relevant behavioral phenotypes, including learning and aggression. Section 5, examples on molecular brain research in man. Section 6, ethical aspects of research in this field.
Download or read book PCR written by Mike McPherson and published by Garland Science. This book was released on 2007-01-25 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
Book Synopsis Human Fungal Pathogens by : Judith E. Domer
Download or read book Human Fungal Pathogens written by Judith E. Domer and published by Springer Science & Business Media. This book was released on 2003-10-07 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reviews and recent results of studies with medically important fungi. Throughout the volume, outstanding mycologists treat the ecological role of pathogenic fungi, how they cause disease, their interactions with the immune system, and their responses to antifungal agents. Most of the presented results are based on molecular biological approaches: areas where classical and descriptive methods are applied are also included.
Book Synopsis The Handbook of Plant Functional Genomics by : Guenter Kahl
Download or read book The Handbook of Plant Functional Genomics written by Guenter Kahl and published by John Wiley & Sons. This book was released on 2008-09-08 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this incisive, concise overview of this booming field, the editors -- two of the leading figures in the field with a proven track record -- combine their expertise to provide an invaluable reference on the topic. Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies. Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary. Equally accessible to both experienced scientists and newcomers to the field.
Book Synopsis Myocardial Preservation and Cellular Adaptation by : D.K. Das
Download or read book Myocardial Preservation and Cellular Adaptation written by D.K. Das and published by Elsevier. This book was released on 1998-12-18 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Living organisms exhibit specific responses when confronted with sudden changes in their environmental conditions. The ability of the cells to acclimate to their new environment is the integral driving force for adaptive modification of the cells. Such adaptation involves a number of cellular and biochemical alteration including metabolic homeostasis and reprogramming of gene expression. Changes in metabolic pathways are generally short-lived and reversible, while the consequences of gene expression are a long-term process and may lead to permanent alternation in the pattern of adaptive responses. The heart possesses remarkable ability to adapt itself against any stressful situation by increasing resistance to the adverse consequences. Stress composes the foundation of many degenerative heart diseases including atherosclerosis, spasm, thrombosis, cardiomyopathy, and congestive heart failure. Based on the concept that excessive stress may play a crucial role in the pathogenesis of ischemic heart disease, attempts were made to design methods for preventing of myocardial injury. Creation of stress reactions by repeated ischemia and reperfusion or subjecting the hearts to heat or oxidative stress enables them to meet the future stress challenge. Repeated stress exposures adapt the heart to withstand more severe stress reactions probably by upregulating the cellular defense and direct accumulation of intracellular mediators, which presumably constitute the material basis of increased adaptation to stress. Thus, the powerful cardioprotective effect of adaptation is likely to originate at the cellular and molecular levels that compose fundamental processes in the prophylaxis of such diseases. Volume six of the Advances in Organ Biology series contains state-of-the-art reviews on myocardial preservation and cellular adaptation from the leading authorities in this subject.
Book Synopsis Fingerprinting Methods Based on Arbitrarily Primed PCR by : Maria R. Micheli
Download or read book Fingerprinting Methods Based on Arbitrarily Primed PCR written by Maria R. Micheli and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes. The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.
Book Synopsis Differential Geometry of Varieties with Degenerate Gauss Maps by : Maks A. Akivis
Download or read book Differential Geometry of Varieties with Degenerate Gauss Maps written by Maks A. Akivis and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book surveys the differential geometry of varieties with degenerate Gauss maps, using moving frames and exterior differential forms as well as tensor methods. The authors illustrate the structure of varieties with degenerate Gauss maps, determine the singular points and singular varieties, find focal images and construct a classification of the varieties with degenerate Gauss maps.
