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Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells
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Book Synopsis Development of a Cytosolic PH Reporter for Tobacco BY2 Cells by : Michael Edward Urbanowski
Download or read book Development of a Cytosolic PH Reporter for Tobacco BY2 Cells written by Michael Edward Urbanowski and published by . This book was released on 2012 with total page 43 pages. Available in PDF, EPUB and Kindle. Book excerpt: The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an important regulator and messenger. Despite this importance, few studies have thoroughly described cytosolic pH patterns as the plant cell progresses through the cell cycle. To investigate pH in plant cells, I chose Nicotiana tabacum (tobacco) Bright Yellow-2 (BY-2) cells as a model system. My research has two aims. First, I will measure and report the interphase cytosolic pH of BY-2 cells. Next, I will assay the cytosolic pH as BY-2 cells progress through mitosis and cytokinesis. I hypothesize that pH patterns are be temporally or spatially associated with structures such as the mitotic spindle or the phragmoplast. To investigate cytosolic pH in BY-2 cells, I will develop a cytosolic pH reporter based on a pH sensitive ratiometric fluorescent dye. This dye will be able to resolve both temporal and spatial changes in pH throughout the cytosol while imposing a minimal amount of stress on BY-2 cells. I found that pH-GFP, a variant of eGFP, had qualities of a robust pH reporter. To introduce the dye, explored biolistic bombardment, Agrobacterium mediated transient transformation, and polyethylene glycol mediated transformation as methods for introducing the pH-GFP gene into BY-2 cells. I observed very few transformation events using these methods and my observations did not support these approaches as suitable for introducing pH-GFP into BY-2 cells.
Book Synopsis Tobacco BY-2 Cells by : Toshiyuki Nagata
Download or read book Tobacco BY-2 Cells written by Toshiyuki Nagata and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first compilation of a wealth of knowledge on tobacco BY-2 cells, often cited as the HeLa cell line of higher plants. Basic issues of cell cycle progression, cytokinesis, cell organization and factors that are involved in these processes are covered in detail. Since the tobacco cell line is used as a tool for research in molecular and cellular biology, several chapters on such studies are also included. Further, changes of primary and secondary metabolites during culture and factors that affect these processes are treated. Last but not least, the so far unpublished historical background of the BY-2 cell line is described. This volume is a must for any scientist working in the field of plant biology.
Book Synopsis Tobacco BY-2 Cells: From Cellular Dynamics to Omics by : Toshiyuki Nagata
Download or read book Tobacco BY-2 Cells: From Cellular Dynamics to Omics written by Toshiyuki Nagata and published by Springer Science & Business Media. This book was released on 2006-09-22 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tobacco BY-2 cell system is a unique model cell line for the study of dynamic features of plant cells. As extension of Volume 53, Tobacco BY-2 Cells, which presented basic aspects of the cell system, this present volume provides a wealth of new approaches. This latest volume in the series is an invaluable source of information for scientists in basic and applied plant biology.
Book Synopsis Vacuolar Compartments by : David G. Robinson
Download or read book Vacuolar Compartments written by David G. Robinson and published by CRC PressI Llc. This book was released on 2000 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research into plant vacuoles has, over the last 5 years, been injected with new data which have already radically changed our concept of the vacuolar compartment in plant cells. In fact, we should no longer speak of the vacuole, but rather of vacuolar compartments. This is a revolutionary change in the way in which the plant cell has traditionally been presented in the biological literature. Vacuolar Compartments is a timely volume that provides a broad overview of our current understanding of the roles of vacuolar compartments in plant cells. Chapters focus on individual function of plant vacuoles and the tonoplast machinery that supports them. Additional chapters present up-to-date information on the identity of different vacuole types and the traffic of proteins to them. The book also provides a basis for comparing the plant system to vacuoles in other organisms. The present challenge is to understand how the separate organelles in the system are generated and maintained by the cell, and to explain at a biochemical level the functions of each vacuole type and the mechanisms by which they are achieved. Vacuolar Compartments is the first book to provide this perspective.
Book Synopsis Plant Cell Walls by : Peter Albersheim
Download or read book Plant Cell Walls written by Peter Albersheim and published by Garland Science. This book was released on 2010-04-15 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell walls are complex, dynamic cellular structures essential for plant growth, development, physiology and adaptation. Plant Cell Walls provides an in depth and diverse view of the microanatomy, biosynthesis and molecular physiology of these cellular structures, both in the life of the plant and in their use for bioproducts and biofuels. Plant Cell Walls is a textbook for upper-level undergraduates and graduate students, as well as a professional-level reference book. Over 400 drawings, micrographs, and photographs provide visual insight into the latest research, as well as the uses of plant cell walls in everyday life, and their applications in biotechnology. Illustrated panels concisely review research methods and tools; a list of key terms is given at the end of each chapter; and extensive references organized by concept headings provide readers with guidance for entry into plant cell wall literature. Cell wall material is of considerable importance to the biofuel, food, timber, and pulp and paper industries as well as being a major focus of research in plant growth and sustainability that are of central interest in present day agriculture and biotechnology. The production and use of plants for biofuel and bioproducts in a time of need for responsible global carbon use requires a deep understanding of the fundamental biology of plants and their cell walls. Such an understanding will lead to improved plant processes and materials, and help provide a sustainable resource for meeting the future bioenergy and bioproduct needs of humankind.
Book Synopsis Auxin and Its Role in Plant Development by : Eva Zažímalová
Download or read book Auxin and Its Role in Plant Development written by Eva Zažímalová and published by Springer. This book was released on 2014-06-26 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Auxin is an important signaling compound in plants and vital for plant development and growth. The present book, Auxin and its Role in Plant Development, provides the reader with detailed and comprehensive insight into the functioning of the molecule on the whole and specifically in plant development. In the first part, the functioning, metabolism and signaling pathways of auxin in plants are explained, the second part depicts the specific role of auxin in plant development and the third part describes the interaction and functioning of the signaling compound upon stimuli of the environment. Each chapter is written by international experts in the respective field and designed for scientists and researchers in plant biology, plant development and cell biology to summarize the recent progress in understanding the role of auxin and suggest future perspectives for auxin research.
Book Synopsis Plant Programmed Cell Death by : Arunika N. Gunawardena
Download or read book Plant Programmed Cell Death written by Arunika N. Gunawardena and published by Springer. This book was released on 2015-10-08 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Programmed cell death (PCD) is a genetically encoded, active process which results in the death of individual cells, tissues, or whole organs. PCD plays an essential role in plant development and defense, and occurs throughout a plant’s lifecycle from the death of the embryonic suspensor to leaf and floral organ senescence. In plant biology, PCD is a relatively new research area, however, as its fundamental importance is further recognized, publications in the area are beginning to increase significantly. The field currently has few foundational reference books and there is a critical need for books that summarizes recent findings in this important area. This book contains chapters written by several of the world’s leading researchers in PCD. This book will be invaluable for PhD or graduate students, or for scientists and researchers entering the field. Established researchers will also find this timely work useful as an up-to-date overview of this fascinating research area.
Book Synopsis Evolution of Membrane Signaling and Trafficking in Plants by : Markus Geisler
Download or read book Evolution of Membrane Signaling and Trafficking in Plants written by Markus Geisler and published by Frontiers E-books. This book was released on 2013-06-12 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins are essential determinants of many biological processes in plants. They function in metabolic processes, signal transduction, transport of small molecules and polymers across endo- and plasma membranes, and intercompartmental trafficking of proteins, lipids, and cell wall components. During these integrative processes, dynamic interactions of membrane proteins with other membrane or soluble components are thought to provide a high degree of flexibility that usually characterizes higher plants. This concept is supported by the recent release of a first, partial Arabidopsis interactome by the Arabidopsis Interactome Mapping Consortium (http://www.sciencemag.org/content/333/6042/601.full.htm). The Arabidopsis interactome reveals a strong enrichment of a few network communities, including those for transmembrane transport and vesicle trafficking. Strikingly, the large transmembrane transport community shares a high amount of proteins with the vesicle trafficking community suggesting a strong physical and functional overlap and interaction.
Book Synopsis Cell-Penetrating Peptides by : Makoto Oba
Download or read book Cell-Penetrating Peptides written by Makoto Oba and published by John Wiley & Sons. This book was released on 2023-02-13 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive reference on the rational design of cell-penetrating peptides enables readers to develop tailor-made peptides for their specific needs. In recent years, cell-penetrating peptides (CPPs) have become valuable tools for the cellular delivery of proteins, nucleic acids, and drugs. These small peptide sequences can be artificially designed and synthesized with custom-made characteristics to mediate the efficient and non-toxic transport of biomolecules, drugs, or nanoparticles into the cell. Cell-Penetrating Peptides: Design, Development, and Applications provides an up-to-date account of the development and use of CPPs for delivering membrane-impermeable bioactive molecules into cells. Bringing together contributions from leading researchers from around the world, this comprehensive volume describes the characteristics and mechanisms of CPPs as well as their application in both medicine, biotechnology and agriculture. Covers rational design and development of cell-penetrating peptides for use in cellular delivery of small molecule drugs, proteins, nucleic acids, and nanoparticles Presents the chemical and biological characteristics of CPP action in vitro and in vivo Describes the structure and design principles of both synthetic and naturally occurring CPPs Discusses key medical applications of CPPs such as oral delivery, intranasal delivery, and clinical trials Cell-Penetrating Peptides: Design, Development, and Applications is an essential resource for biochemists, medicinal chemists, molecular biologists, biotechnologists, and researchers studying CPPs in both academia and industry.
Book Synopsis Reactive Oxygen Species in Plant Signaling by : Luis A. del Río
Download or read book Reactive Oxygen Species in Plant Signaling written by Luis A. del Río and published by Springer Science & Business Media. This book was released on 2009-06-22 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oxygen (O ) appeared in significant amounts in the Earth’s atmosphere over 2. 2 2 billion years ago, largely due to the evolution of photosynthesis by cyanobacteria (Halliwell 2006). The O molecule is a free radical, as it has two impaired electrons 2 that have the same spin quantum number. This spin restriction makes O prefer to 2 accept its electrons one at a time, leading to the generation of the so-called reactive oxygen species (ROS). The chemical nature of these species dictates that they can create damage in cells. This has contributed to the creation of the “oxidative stress” concept; in this view, ROS are unavoidable toxic products of O metabolism and 2 aerobic organisms have evolved antioxidant defences to protect against this tox- ity (Halliwell 1981; Fridovich 1998). Indeed, even in present-day plants, which are full of antioxidants, much of the protein synthetic activity of chloroplasts is used to replace oxidatively damaged D1 and other proteins (Halliwell 2006). Yet, the use of the “oxidative stress” term implies that ROS exert their effects through indiscriminate widespread inactivation of cellular functions. In this context, ROS must not be able to react with lipids, proteins or nucleic acids in order to avoid any damage to vital cellular components. However, genetic evidence has suggested that, in planta, purely physicoche- cal damage may be more limited than previously thought (Foyer and Noctor 2005).
Book Synopsis Regulation of Pollen Tube Growth, Volume II by : Stefano Del Duca
Download or read book Regulation of Pollen Tube Growth, Volume II written by Stefano Del Duca and published by Frontiers Media SA. This book was released on 2023-07-26 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Signal Transduction in Plant Growth and Development by : Desh P.S. Verma
Download or read book Signal Transduction in Plant Growth and Development written by Desh P.S. Verma and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant growth and development is controlled by various environmental cues that are sensed by the plant via various signal transduction pathways coupled to specific response. Some of these pathways are conserved from yeast to plants being regulated by various kinases and phosphatases. In addition, plants have many unique pathways that transduce to specific signals such as light, phytohormones and oligosaccharides. This volume highlights some of the examples of the plant signal transduction machinery opening new vistas in research on plant growth and development. The new technologies including the use of bacteria, yeast and Arabidopsis as functional complementation systems are providing proof of function of many of the proteins that show homology to those from other organisms. These studies will eventually lead to improvement of crop plants and use of plants as a new resource for producing desirable products to meet the growing needs of mankind.
Book Synopsis Regulatory Mechanisms of Leaf Senescence Under Environmental Stresses by : Yasuhito Sakuraba
Download or read book Regulatory Mechanisms of Leaf Senescence Under Environmental Stresses written by Yasuhito Sakuraba and published by Frontiers Media SA. This book was released on 2020-10-13 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Book Synopsis Proceedings of the National Academy of Sciences of the United States of America by : National Academy of Sciences (U.S.).
Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.). and published by . This book was released on 2007 with total page 1172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Boron in Plant and Animal Nutrition by : Heiner E. Goldbach
Download or read book Boron in Plant and Animal Nutrition written by Heiner E. Goldbach and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Boron '97 meeting was a great success in summarising all recent developments in basic and applied research on boron's function, especially in plants. New techniques have since been developed and new insight has been gained into the role of boron in plant and animal metabolism. Nevertheless, there were still lots of open questions. The aim of the present workshop held in Bonn as a satellite meeting to the International Plant Nutrition Colloquium was thus to gather all actual information which has been gained since the Boron '97 meeting and to compile knowledge, both from animal and plant sciences. Furthermore, applied aspects had to be addressed too, as there is an increasing awareness of boron deficiencies even in crops such as wheat, which have formerly not been considered as responsive to boron application. Genetic differences in boron demand and efficiency within one species are a further important topic which has gained importance since the 1997 meeting. More in-depth knowledge on the mechanisms of boron efficiency are required as an increased efficiency will be one major possibility to maintain and improve crop yields for resource-poor farmers. Nevertheless, it has also clearly been shown that an adequate supply of boron is needed to obtain high yields of crops with a high quality, and that a sustainable agriculture has to provide an adequate boron supply to compensate for inevitable losses through leaching (especially in the humid tropics and temperate regions) and for the boron removal by the crop.
Book Synopsis Endocytosis in Plants by : Jozef Šamaj
Download or read book Endocytosis in Plants written by Jozef Šamaj and published by Springer Science & Business Media. This book was released on 2012-10-02 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
Book Synopsis Plant Electrophysiology by : Alexander G. Volkov
Download or read book Plant Electrophysiology written by Alexander G. Volkov and published by Springer Science & Business Media. This book was released on 2007-04-19 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles new findings in plant electrophysiology from the work of internationally renowned experts in the fields of electrophysiology, bio-electrochemistry, biophysics, signal transduction, phloem transport, tropisms, ion channels, plant electrochemistry, and membrane transport. Opening with a historical introduction, the book reviews methods in plant electrophysiology, introducing such topics as measuring membrane potentials and ion fluxes, path-clamp technique, and electrochemical sensors. The coverage includes experimental results and their theoretical interpretation.
