Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Development And Application Of Multi Functionalized Mesoporous Silica Nanomaterials In Intracellular Drug Delivery And Heterogeneous Catalysis
Download Development And Application Of Multi Functionalized Mesoporous Silica Nanomaterials In Intracellular Drug Delivery And Heterogeneous Catalysis full books in PDF, epub, and Kindle. Read online Development And Application Of Multi Functionalized Mesoporous Silica Nanomaterials In Intracellular Drug Delivery And Heterogeneous Catalysis ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Development and Application of Multi-functionalized Mesoporous Silica Nanomaterials in Intracellular Drug Delivery and Heterogeneous Catalysis by : Chih-Hsiang Tsai
Download or read book Development and Application of Multi-functionalized Mesoporous Silica Nanomaterials in Intracellular Drug Delivery and Heterogeneous Catalysis written by Chih-Hsiang Tsai and published by . This book was released on 2012 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Surface Functionalized Mesoporous Silica Nanoparticles for Intracellular Drug Delivery by : Juan Luis Vivero-Escoto
Download or read book Surface Functionalized Mesoporous Silica Nanoparticles for Intracellular Drug Delivery written by Juan Luis Vivero-Escoto and published by . This book was released on 2009 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mesoporous Materials for Drug Delivery and Theranostics by : Valentina Cauda
Download or read book Mesoporous Materials for Drug Delivery and Theranostics written by Valentina Cauda and published by MDPI. This book was released on 2020-12-17 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesoporous materials are capturing great interest thanks to their exceptional surface area, uniform and tunable pore size, ease surface functionalization, thus enabling broad series of intervention in the field of nanomedicine. Since many years, these aspects foster a deep investigation on mesoporous nanoparticles, to design and fabricate biocompatible, smart and stimuli-responsive nanotools for controlled drug- or gene-delivery, theranostics applications, in particular for cancer therapy, and tissue engineering. This Book is thus dedicated to the most recent advances in the field, collecting research papers and reviews. It spans from the synthesis and characterization of the mesoporous material, especially those made of silica, silicon and bioactive glasses, to their functionalization with smart gate-keepers, reporter molecules or targeting ligands, up to their in-vitro applications in the nanomedicine field.
Book Synopsis Design, Synthesis, Multifunctionalization and Biomedical Applications of Multifunctional Mesoporous Silica-Based Drug Delivery Nanosystems by : Yu Chen
Download or read book Design, Synthesis, Multifunctionalization and Biomedical Applications of Multifunctional Mesoporous Silica-Based Drug Delivery Nanosystems written by Yu Chen and published by Springer. This book was released on 2015-10-14 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis reports on essential advances in the design, synthesis and biomedical applications of multifunctional Mesoporous Silica Nanoparticles (MSNs). It provides several examples of multifunctional MSN-based drug delivery nanosystems and demonstrates successful synergistic cancer therapies combining MSNs and high-intensity focused ultrasound. The book will especially be of interest to researchers and graduate students in the fields of biomaterials, biology, chemistry, medicine and the life sciences who are working to develop new methods and technologies to combat cancer.
Book Synopsis Functionalized Mesoporous Silica Nanoparticles (MSNs) for Applications in Drug Delivery and Catalysis by : Wei Huang
Download or read book Functionalized Mesoporous Silica Nanoparticles (MSNs) for Applications in Drug Delivery and Catalysis written by Wei Huang and published by . This book was released on 2010 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mesoporous Silica Nanoparticles by : Sachin Namdeo Kothawade
Download or read book Mesoporous Silica Nanoparticles written by Sachin Namdeo Kothawade and published by . This book was released on 2024-11-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesoporous silica comprehensively covers the importance and applications of mesoporous silica nanoparticles in the field of nanoscience and nanotechnology. The book delves into the synthesis and characterization of mesoporous silica nanoparticles, discussing various synthesis methods and characterization techniques employed in their production. It explores the properties and structure of mesoporous silica nanoparticles, including their porosity, surface area, structural features, and tunability. It discusses mechanical, thermal, and optical properties. The applications of mesoporous silica nanoparticles in drug delivery are covered in detail, focusing on controlled release systems, targeted drug delivery, and theranostic applications.The catalytic applications of mesoporous silica nanoparticles are examined, including the use of these nanoparticles as supported catalysts in catalytic reactions, with discussions on reaction mechanisms. The book also explores the sensing and biosensing applications of mesoporous silica nanoparticles, including optical and electrochemical sensing, bioanalytical applications, and detection of biomolecules and environmental pollutants. Surface functionalization techniques for mesoporous silica nanoparticles are discussed, highlighting the importance of tailoring their properties for specific applications. Biocompatibility and toxicity considerations are addressed, providing insights into the assessment of biocompatibility, toxicity evaluation, mitigation strategies, and regulatory considerations. The future directions and emerging trends in mesoporous silica nanoparticle research are explored, along with interdisciplinary approaches, challenges, and opportunities in the field. The book concludes by summarizing the key findings and discussing the overall significance of mesoporous silica nanoparticles in nanoscience and nanotechnology. The references section provides a comprehensive list of sources used throughout the book for further exploration. The book serves as an essential resource for researchers, professionals, and students interested in understanding the synthesis, characterization, properties, and diverse applications of mesoporous silica nanoparticles in the realm of nanoscience and nanotechnology.
Book Synopsis Drug Delivery Using Nanomaterials by : Yasser Shahzad
Download or read book Drug Delivery Using Nanomaterials written by Yasser Shahzad and published by CRC Press. This book was released on 2022-01-18 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: After the drug discovery and development process, designing suitable formulations to safely deliver the optimum dose, while avoiding side effects, has been a constant challenge, especially when drugs are very toxic and have poor solubility and undesirable clearance profiles. With recent advances in synthetic technologies, nanoparticles can be custom-made from a variety of advanced materials to mimic the bioenvironment and can be equipped with various targeting and imaging moieties for site-specific delivery and real-time imaging. Drug Delivery Using Nanomaterials covers advancements in the field of nanoparticle-based drug-delivery systems, along with all the aspects needed for a successful and marketable nanoformulation. FEATURES Offers a general overview of the entire process involved in the synthesis and characterization of pharmaceutical nanoparticles Covers a broad range of synthetic materials for developing nanoformulations customized for specific disease states, target organs, and drugs Every chapter sequentially builds, providing a progressive pathway from classical nanoparticles to the more advanced to be used as a full drug product by consumers Provides information in a bottom-up manner in that definitions and explanations of relevant background information serve as a framework for understanding advanced concepts This user-friendly reference is aimed at materials engineers, chemical engineers, biomedical engineers, pharmaceutical scientists, chemists, and others working on advanced drug delivery, from academia as well as industry.
Book Synopsis Biological Applications and Transmission Electron Microscopy Investigations of Mesoporous Silica Nanoparticles by :
Download or read book Biological Applications and Transmission Electron Microscopy Investigations of Mesoporous Silica Nanoparticles written by and published by . This book was released on 2006 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: The research presented and discussed within involves the development of novel biological applications of mesoporous silica nanoparticles (MSN) and an investigation of mesoporous material by transmission electron microscopy (TEM). Mesoporous silica nanoparticles organically functionalized shown to undergo endocytosis in cancer cells and drug release from the pores was controlled intracellularly and intercellularly. Transmission electron microscopy investigations demonstrated the variety of morphologies produced in this field of mesoporous silica nanomaterial synthesis. A series of room-temperature ionic liquid (RTIL) containing mesoporous silica nanoparticle (MSN) materials with various particle morphologies, including spheres, ellipsoids, rods, and tubes, were synthesized. By changing the RTIL template, the pore morphology was tuned from the MCM-41 type of hexagonal mesopores to rotational moire type of helical channels, and to wormhole-like porous structures. These materials were used as controlled release delivery nanodevices to deliver antibacterial ionic liquids against Escherichia coli K12. The involvement of a specific organosiloxane function group, covalently attached to the exterior of fluorescein doped mesoporous silica nanoparticles (FITC-MSN), on the degree and kinetics of endocytosis in cancer and plant cells was investigated. The kinetics of endocystosis of TEG coated FITC-MSN is significantly quicker than FITC-MSN as determined by flow cytometry experiments. The fluorescence confocal microscopy investigation showed the endocytosis of TEG coated-FITC MSN triethylene glycol grafted fluorescein doped MSN (TEG coated-FITC MSN) into both KeLa cells and Tobacco root protoplasts. Once the synthesis of a controlled-release delivery system based on MCM-41-type mesoporous silica nanorods capped by disulfide bonds with superparamagnetic iron oxide nanoparticles was completed. The material was characterized by general methods and the dosage and kinetics of the antioxidant dependent release was measured. Finally, the biological interaction of the material was determined along with TEM measurements. An electron investigation proved that the pore openings of the MSN were indeed blocked by the Fe3O4 nanoparticles. The biological interaction investigation demonstrated Fe3O4-capped MSN endocytosis into HeLa cells. Not only does the material enter the cells through endocytosis, but it seems that fluorescein was released from the pores most probably caused by disulfide bond reducing molecules, antioxidants. In addition to endocytosis and release, the Fe3O4-capped MSN propelled the cells across a cuvette upon induction of a magnet force. Finally, an important aspect of materials characterization is transmission electron microscopy. A TEM investigation demonstrated that incorporating different functional groups during the synthesis (co-condensation) changed the particle and pore morphologies.
Book Synopsis Silica-coated Magnetic Nanoparticles by : Mariela A. Agotegaray
Download or read book Silica-coated Magnetic Nanoparticles written by Mariela A. Agotegaray and published by Springer. This book was released on 2017-01-04 with total page 93 pages. Available in PDF, EPUB and Kindle. Book excerpt: This brief offers a comprehensive discussion of magnetic targeted drug delivery of silica-coated nanodevices. Focusing on the latest trend in pharmaceutical applications of these nanodevices, a multidisciplinary overview is displayed, from synthesis and design to pharmacokenetics, biodistribution and toxicology. Chapters include design of silica-coated magnetic nanodevices; techniques for drug loading with features applicable to biological systems; synthesis, characterization and the assessment of biomedical issues with both in vitro and in vivo experiments. Applications in the treatment of different localized diseases are also addressed in order to present the potential use of these nanosystems as global, commercially available therapeutics.
Book Synopsis Morphological Control of Multifunctionalized Mesoporous Silica Nanomaterials for Catalysis Applications by : Seong Huh
Download or read book Morphological Control of Multifunctionalized Mesoporous Silica Nanomaterials for Catalysis Applications written by Seong Huh and published by . This book was released on 2004 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: I found an efficient method to control the morphology of the organically monofunctionalized mesoporous silica materials by introducing different types of organoalkoxysilanes in a base-catalyzed co-condensation reaction. The monofunctionalized materials exhibit different particle morphologies relative to the pure MCM-41 material. The concentration dependence of the morphology is a critical factor to determine the final particle shape. A proposed mechanism of the shape evolution is also offered. After understanding the role of organoalkoxysilanes in producing various well-shaped nanomaterials, I also obtained a series of bifunctional mesoporous silica materials with certain particle morphology. A series of bifunctional mesoporous silica nanospheres (MSNs) whose physicochemical properties was investigated via solid state NMR techniques and Cu2 adsorption capacity tests. The ratio of two different organic groups inside of mesopores of these MSNs could be fine-tuned. These MSNs serve as a useful model system to study substrate selectivity in catalytic reactions and sorption phenomena. For example, the Cu2+ adsorption capacity of these materials was dictated by the chemical nature of the mesopores generated by the different organic functional groups. An investigation of the substrate selectivity of the bifunctionalized MSNs in a competitive nitroaldol reaction using an equimolar amount of two competing 4-nitrobenzaldehyde derivatives was performed. Shape-controlled bifunctional MSNs were employed as the catalysts. The properties of the MSNs were investigated using various spectroscopic methods and electron microscopy. The more hydrophobic the surface organic groups are, the higher the ratio of hydrophobic final product. This is the first example to demonstrate the selection of substrate using physicochemical nature of the mesopore surface other than the conventional shape selection in zeolite systems. I also created a cooperative dual catalyst system that is capable of activating two different substrates in aldol reaction, Henry reaction and cyanosilylation. One catalytic group activates the nucleophile, another organic group simultaneously activates the electrophile to enhance the total reaction rate. I systematically varied the amount of two organic groups and performed the three model reactions to compare rate enhancements.
Book Synopsis Biological Applications and Transmission Electron Microscopy Investigations of Mesoporous Silica Nanoparticles by : Brian G. Trewyn
Download or read book Biological Applications and Transmission Electron Microscopy Investigations of Mesoporous Silica Nanoparticles written by Brian G. Trewyn and published by . This book was released on 2006 with total page 3472 pages. Available in PDF, EPUB and Kindle. Book excerpt: The research presented and discussed within involves the development of novel biological applications of mesoporous silica nanoparticles (MSN) and an investigation of mesoporous material by transmission electron microscopy (TEM). Mesoporous silica nanoparticles organically functionalized shown to undergo endocytosis in cancer cells and drug release from the pores was controlled intracellularly and intercellularly. Transmission electron microscopy investigations demonstrated the variety of morphologies produced in this field of mesoporous silica nanomaterial synthesis. A series of room-temperature ionic liquid (RTIL) containing mesoporous silica nanoparticle (MSN) materials with various particle morphologies, including spheres, ellipsoids, rods, and tubes, were synthesized. By changing the RTIL template, the pore morphology was tuned from the MCM-41 type of hexagonal mesopores to rotational moire type of helical channels, and to wormhole-like porous structures. These materials were used as controlled release delivery nanodevices to deliver antibacterial ionic liquids against Escherichia coli K12. The involvement of a specific organosiloxane function group, covalently attached to the exterior of fluorescein doped mesoporous silica nanoparticles (FITC-MSN), on the degree and kinetics of endocytosis in cancer and plant cells was investigated. The kinetics of endocystosis of TEG coated FITC-MSN is significantly quicker than FITC-MSN as determined by flow cytometry experiments. The fluorescence confocal microscopy investigation showed the endocytosis of TEG coated-FITC MSN triethylene glycol grafted fluorescein doped MSN (TEG coated-FITC MSN) into both KeLa cells and Tobacco root protoplasts. Once the synthesis of a controlled-release delivery system based on MCM-41-type mesoporous silica nanorods capped by disulfide bonds with superparamagnetic iron oxide nanoparticles was completed. The material was characterized by general methods and the dosage and kinetics of the antioxidant dependent release was measured. Finally, the biological interaction of the material was determined along with TEM measurements. An electron investigation proved that the pore openings of the MSN were indeed blocked by the Fe{sub 3}O{sub 4} nanoparticles. The biological interaction investigation demonstrated Fe{sub 3}O{sub 4}-capped MSN endocytosis into HeLa cells. Not only does the material enter the cells through endocytosis, but it seems that fluorescein was released from the pores most probably caused by disulfide bond reducing molecules, antioxidants. In addition to endocytosis and release, the Fe{sub 3}O{sub 4}-capped MSN propelled the cells across a cuvette upon induction of a magnet force. Finally, an important aspect of materials characterization is transmission electron microscopy. A TEM investigation demonstrated that incorporating different functional groups during the synthesis (co-condensation) changed the particle and pore morphologies.
Book Synopsis Multifunctionalized Mesoporous Silica Nanoparticles for Selective Catalysis by : Hung-Ting Chen
Download or read book Multifunctionalized Mesoporous Silica Nanoparticles for Selective Catalysis written by Hung-Ting Chen and published by . This book was released on 2007 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: The research presented and discussed within this dissertation involves the development of multifunctional mesoporous silica nanoparticles (MSN), and an investigation of performance of those MSN materials for heterogeneous catalysis.
Book Synopsis Advanced Theranostic Materials by : Ashutosh Tiwari
Download or read book Advanced Theranostic Materials written by Ashutosh Tiwari and published by John Wiley & Sons. This book was released on 2015-06-30 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book is covers the recent advances in the development on the regulation of such theragnosis system and their biomedical perspectives to act as a future nanomedicine. Advanced Theranostics Materialsis written by a distinguished group of contributors and provides comprehensive coverage of the current literature, up-to-date overview of all aspects of advanced theranostics materials ranging from system biology, diagnostics, imaging, image-guided therapy, therapeutics, biosensors, and translational medicine and personalized medicine, as well as the much broader task of covering most topics of biomedical research. The books focusses on the following topics: Part 1: System biology and translational medicine Aberrant Signaling Pathways: Hallmark of Cancer Cells and Target for Nanotherapeutics Application of Nanoparticles in Cancer Treatment Biomacromolecule-Gated Mesoporous Silica Drug Delivery Systems Construction of Functional DNA Nanostructures for Theranostic Applications Smart Polypeptide Nanocarriers for Malignancy Therapeutics Part 2: Imaging and therapeutics Dimercaptosuccinic acid-coated magnetic nanoparticles as a localized delivery system in cancer immunotherapy Cardiovascular nanomedicine Chitosan-based systems for sustained drug release Nanocapsules in biomedicine: promises and challenges Chitosan-based polyelectrolyte complexes: characteristics and application in formulation of particulate drug carriers Part 3: Diagnostics and featured prognostics Non-invasive Glucose Biosensors based on Nanomaterials Self/directed Assembly of Nanoparticles: A review on various approaches Ion exchangers – an open window for the development of advanced materials with pharmaceutical and medical applications New Titanium Alloys for Biomedical Applications
Book Synopsis Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection by :
Download or read book Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection written by and published by . This book was released on 2004 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: The central theme of this dissertation is represented by the versatility of mesoporous silica nanomaterials in various applications such as catalysis and bio-applications, with main focus on biological applications of Mesoporous Silica Nanospheres (MSN). The metamorphosis that we impose to these materials from catalysis to sensing and to drug and gene delivery is detailed in this dissertation. First, we developed a synthetic method that can fine tune the amount of chemically accessible organic functional groups on the pores surface of MSN by exploiting electrostatic and size matching between the cationic alkylammonium head group of the cetyltrimethylammonium bromide (CTAB) surfactant and various anionic organoalkoxysilane precursors at the micelle-water interface in a base-catalyzed condensation reaction of silicate. Aiming nature imitation, we demonstrated the catalytic abilities of the MSNs, We utilized an ethylenediamine functional group for chelating Cu2+ as a catalytic functional group anchored inside the mesopores. Thus, a polyalkynylene-based conducting polymer (molecular wire) was synthesized within the Cu-functionalized MSNs silica catalyst. For sensing applications, we have synthesized a poly(lactic acid) coated mesoporous silica nanosphere (PLA-MSN) material that serves as a fluorescence sensor system for detection of amino-containing neurotransmitters in neutral aqueous buffer. We exploited the mesoporosity of MSNs for encapsulating pharmaceutical drugs. We examined bio-friendly capping molecules such as polyamidoamine dendrimers of generations G2 to G4, to prevent the drug leaching. Next, the drug delivery system employed MSNs loaded with Doxorubicin, an anticancer drug. The results demonstrated that these nano-Trojan horses have ability to deliver Doxorubicin to cancer cells and induce their death. Finally, to demonstrate the potential of MSN as an universal cellular transmembrane nanovehicle, we anchored positively charged dendrimers on the surface of MSN and utilize them to complex cationic DNA. The p-EGFP-CI gene-coated MSN nanocomposite was able to transfect cancer cell lines, such as human HeLa and CHO cancer cell lines. The gene carrier ability of MSNs was further proved by transfecting primary cells and cotransfecting of two different genes in cancer cell lines. In sum, MSN are versatile partners in several types of applications.
Book Synopsis Mesoporous Silica-based Nanomaterials and Biomedical Applications - Part A by :
Download or read book Mesoporous Silica-based Nanomaterials and Biomedical Applications - Part A written by and published by Academic Press. This book was released on 2018-09-20 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer Therapy and Diagnosis, Part A, Volume 43 in The Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters on Mesoporous silica nanoparticle synthesis, Periodic mesoporous organosilica, Nanovalves and other nanomachine-equipped nanoparticles and controlled release, Two-photon light control and photodynamic therapy, Biodegradable PMO nanoparticles, Cationic mesoporous silica and protein delivery, Drug loading, stimuli-responsive delivery and cancer treatment, Animal models and cancer therapy, siRNA delivery and TWIST shutdown for ovarian cancer treatment, and TBC (mesoporous silica nanoparticles and cancer therapy or biodistribution of MSN). Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in The Enzymes series Updated release includes the latest information on Cancer Therapy and Diagnosis
Book Synopsis Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection by : Daniela Rodica Radu
Download or read book Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection written by Daniela Rodica Radu and published by . This book was released on 2004 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: The central theme of this dissertation is represented by the versatility of mesoporous silica nanomaterials in various applications such as catalysis and-applications, with main focus on biological applications of Mesoporous Silica Nanospheres (MSN). The metamorphosis that we impose to these materials from catalysis to sensing and to drug and gene delivery is detailed in this dissertation. First, we developed a synthetic method that can fine tune the amount of chemically accessible organic functional groups on the pores surface of MSN by exploiting electrostatic and size matching between the cationic alkylammonium head group of the CTAB surfactant and various anionic organoalkoxysilane precursors at the micelle-water interface in a base-catalyzed condensation reaction of silicate. Aiming nature imitation, we demonstrated the catalytic abilities of the MSNs. We utilized an ethylenediamine functional group for chelating Cu2 as a catalytic functional group anchored inside the mesopores. Thus, a polyalkynylene-based conducting polymer (molecular wire) was synthesized within the Cu-functionalized MSNs silica catalyst. For sensing applications, we have synthesized a poly(lactic acid) coated mesoporous silica nanosphere (PLA-MSN) material that serves as a fluorescence sensor system for detection of amino-containing neurotransmitters in neutral aqueous buffer. We exploited the mesoporosity of MSNs for encapsulating pharmaceutical drugs. We examined bio-friendly capping molecules such as polyamidoamine dendrimers of generations G2 to G4, to prevent the drug leaching. Next, the drug delivery system employed MSNs loaded with Doxorubicin, an anticancer drug. The results demonstrated that these nano-Trojan horses have ability to deliver Doxorubicin to cancer cells and induce their death. Finally, to demonstrate the potential of MSN as an universal cellular transmembrane nanovehicle, we anchored positively charged dendrimers on the surface of MSN and utilize them to complex cationic DNA. The p-EGFP-C1 gene-coated MSN nanocomposite was able to transfect cancer cell lines, such as human HeLa and CHO cancer cell lines. The gene carrier ability of MSNs was further proved by transfecting primary cells and cotransfecting of two different genes in cancer cell lines. In sum, MSN are versatile partners in several types of applications, as demonstrated in the 164 pages of my dissertation.
Book Synopsis Multifunctional Mesoporous Silica Nanospheres for Biosensor, Stimuli-responsive Controlled-release Drug Delivery Carriers and Gene Transfection Vectors by : Cheng-Yu Lai
Download or read book Multifunctional Mesoporous Silica Nanospheres for Biosensor, Stimuli-responsive Controlled-release Drug Delivery Carriers and Gene Transfection Vectors written by Cheng-Yu Lai and published by . This book was released on 2004 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structurally well-defined mesoporous silica materials with tunable pore size and narrow pore-size distribution have attracted much attention for their potential applications in sensing, drug delivery and gene transfer. A series of mesoporous silica nanosphere-based (MSN) nano-devices were synthesized and utilized for the aforementioned applications. For sensing application, we provide a first example in utilization of a multifunctionalized mesoporous silica material to selectively detect dopamine. A second application, rooted in this work, is the selective detection of amino-containing neurotransmitters under physiological condition. The drug delivery applications have been built around a controlled release drug delivery device. In the original conception, the system is using surface-derivatized cadmium sulfide (CdS) nanocrystals as chemically removable caps to encapsulate several drug molecules and neurotransmitters inside the organically functionalized MSN mesoporous framework. To study the cap-dependence release profile, adenosine 5-triphosphate (ATP) molecules were encapsulated in the MSNs followed by capping of the mesopores with chemically removable caps, such as CdS nanoparticles and poly(amido amine) dendrimers (PAMAM). A luciferase chemiluminescence imaging method was employed to investigate the kinetics and mechanism of the ATP release with various disulfide-reducing agents as uncapping triggers in real time. By varying the chemical nature of the "cap" and "trigger" molecules in our MSN system, we observed that the release profiles could be regulated. Further, the PAMAM dendrimers of low generations were utilized as MSN caps for drug delivery. The biocompatibility and delivery efficiency of the later MSN system in interaction with HeLa cervical cancer cells were demonstrated. The system renders the possibility to serve as a universal transmembrane carrier for intracellular drug delivery and imaging applications. This is the first uptake study of MCM-41 type mesoporous silicas into eukaryotic cells. The last application presented in the current dissertation is gene delivery. A MSN based gene transfection system was developed, where second generation (G2) PAMAMs were covalently attached to the surface of MSN. The G2-PAMAM-capped MSN material (G2-MSN) was able to complex with a plasmid DNA (pEGFP-C1). The gene transfection efficacy, uptake mechanism, and biocompatibility of the G2-MSN system with various cell types were investigated.
