Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS).

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Publisher :
ISBN 13 :
Total Pages : 17 pages
Book Rating : 4.:/5 (742 download)

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Book Synopsis Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS). by :

Download or read book Determination of MS2 Bacteriophage Stability at High Temperatures Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2005 with total page 17 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third report in a series of reports that investigates the survivability of the MS2 virus in harsh environments. The first two reports study the effects of pH on the virus. In this report, we study the effect of elevated temperatures on the virus. The stability of the virus was studied by counting the number of virus particles using the Integrated Virus Detection System (IVDS). Test results show that the virus particles are relatively stable for a limited time (up to 0.50 hr) at temperatures as high as 63 degrees C. Longer exposure or exposure to higher temperatures, results in quick breakdown of the virus particles.

Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS).

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ISBN 13 :
Total Pages : 13 pages
Book Rating : 4.:/5 (227 download)

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Book Synopsis Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS). by :

Download or read book Determination of MS2 Bacteriophage Stability at Low PH Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2006 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report describes testing of the survivability of MS2 virus under harsh conditions using the Integrated Virus Detection System (IVDS). In this study, the MS2 was subjected to a highly acidic and oxidative environment. Judging by the concentration of the virus particles, the virus survived 66 hr in 0.1 N HNO3 solution without any signs of diminution in the number of virus particles. This suggests that viruses might be able to survive such harsh environmental conditions.

Determination of MS2 Bacteriophage Stability at High PH Using the Integrated Virus Detection System (IVDS).

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Publisher :
ISBN 13 :
Total Pages : 16 pages
Book Rating : 4.:/5 (227 download)

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Book Synopsis Determination of MS2 Bacteriophage Stability at High PH Using the Integrated Virus Detection System (IVDS). by :

Download or read book Determination of MS2 Bacteriophage Stability at High PH Using the Integrated Virus Detection System (IVDS). written by and published by . This book was released on 2006 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: A sample of MS2 stock solution was prepared by filtering with reverse osmosis water in a cross flow ultra filtration system. The cross flow ultra filtration system, which uses a molecular weight cut-off (MWCO) filter of l00K Daltons, circulates the MS2 stock solution with the water. The filter allows any impurities (e.g. growth media, miscellaneous salts, and proteins) to pass through the filter but retain the MS2. The sample of MS2 is concentrated and retained for testing at high pH to determine the survival rate. The treated MS2 samples were analyzed using the Integrated Virus Detection System (IVDS). The detection stage of the IVDS consists of an electrospray unit to inject samples into the detector, a Differential Mobility Analyzer and a Condensate Particle Counter.

Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage

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Publisher :
ISBN 13 :
Total Pages : 15 pages
Book Rating : 4.:/5 (742 download)

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Book Synopsis Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage by :

Download or read book Stability of the Integrated Virus Detection System (VDS) Electrospray Module During Analysis of MS2 Bacteriophage written by and published by . This book was released on 2005 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report characterizes the stability of the electrospray module of the IVDS system while analyzing a sample of MS2 bacteriophage. The electrospray parameters were varied across the stable range, and resultant output from the detector was analyzed.

Integrated Virus Detection

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Publisher : CRC Press
ISBN 13 : 1482230054
Total Pages : 260 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Integrated Virus Detection by : Charles H. Wick

Download or read book Integrated Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2014-11-13 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multiple viruses can be detected concurrently using the Integrated Virus Detection System (IVDS). Integrated Virus Detection describes this technology and provides many examples of applications including a chapter on viruses found in honeybees with descriptions of seasonal and yearly variation. This straightforward technology can be used to detect known, unknown, and unsequenced viruses collected from environmental and other complex biological sources. This book summarizes more than ten US patents issued for the invention of the IVDS, which is the common name of the electrospray–differential mobility analyzer method. The IVDS is powering mankind’s ability to rapidly detect, measure, and monitor viruses as well as virus-like particles. Three facts make rapid detection possible: virus size, which ranges from 20 to 800 nm.; disparity in each virus species’ particle size thus allowing size data to be used for detection and preliminary identification; and the fact that virus particle density is distinct from other nanoparticles. The IVDS can ascertain the absence of virion particles, thus presenting compelling evidence of a true negative, which is useful in verifying decontamination and other processes. In addition, large numbers of samples may be processed in an automated fashion, providing an excellent means to prescreen them for judicious targeting of subsequent tests such as PCR or the discriminating method for identifying microbes, which is mass spectrometry proteomics.* The book is helpful to anyone interested in virus detection, especially in situations where many viral types may coexist. *Identifying Microbes by Mass Spectrometry Proteomics (CRC Press 2013)

Virus Detection

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Publisher : CRC Press
ISBN 13 : 1000868303
Total Pages : 158 pages
Book Rating : 4.0/5 (8 download)

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Book Synopsis Virus Detection by : Charles H. Wick

Download or read book Virus Detection written by Charles H. Wick and published by CRC Press. This book was released on 2023-05-03 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viruses do not behave as other microbes; their life cycles require infecting healthy cells, commandeering their cellular apparatus, replicating and then killing the host cell. Methods for virus detection and identification have been developed only in the past few decades. These recently developed methods include molecular, physical, and proteomic techniques. All these approaches (Electron Microscopy, Molecular, Direct Counting, and Mass Spectrometry Proteomics) to detection and identification are reviewed in this succinct volume. It is written in approachable language with enough detail for trained professionals to follow and want to recommend to others. Key Features Covers common detection methods Reviews the history of detection from antiquity to the present Documents the strengths and weaknesses of various detection methods Describes how to detect newly discovered viruses Recommends specific applications for clinical, hospital, environmental, and public health uses

Bacteriophage

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Publisher : Caister Academic Press Limited
ISBN 13 :
Total Pages : 362 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Bacteriophage by : Stephen Mc Grath

Download or read book Bacteriophage written by Stephen Mc Grath and published by Caister Academic Press Limited. This book was released on 2007 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by eminent international researchers actively involved in the disparate areas of bacteriophage research this book focuses on the current rapid developments in this exciting field.

Molecular Diagnostics: Promises and Possibilities

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Publisher : Springer Science & Business Media
ISBN 13 : 9048132614
Total Pages : 527 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis Molecular Diagnostics: Promises and Possibilities by : Mousumi Debnath

Download or read book Molecular Diagnostics: Promises and Possibilities written by Mousumi Debnath and published by Springer Science & Business Media. This book was released on 2010-01-29 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.

Microfiltration and Ultrafiltration

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Publisher : Routledge
ISBN 13 : 1351431501
Total Pages : 654 pages
Book Rating : 4.3/5 (514 download)

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Book Synopsis Microfiltration and Ultrafiltration by : Zeman

Download or read book Microfiltration and Ultrafiltration written by Zeman and published by Routledge. This book was released on 2017-11-22 with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt: Integrates knowledge on microfiltration and ultrification, membrane chemistry, and characterization methods with the engineering and economic aspects of device performance, device and module design, processes, and applications. The text provides a discussion of membrane fundamentals and an analytical framework for designing and developing new filtrations systems for a broad range of technologically important functions. It offers information on membrane liquid precursors, fractal and stochastic pore space analysis, novel and advanced module designs, and original process design calculations.

Proteomics for Biological Discovery

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Publisher : John Wiley & Sons
ISBN 13 : 0470007737
Total Pages : 361 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Proteomics for Biological Discovery by : Timothy D. Veenstra

Download or read book Proteomics for Biological Discovery written by Timothy D. Veenstra and published by John Wiley & Sons. This book was released on 2006-06-12 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.

Proteomics for Biological Discovery

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Publisher : John Wiley & Sons
ISBN 13 : 1119081726
Total Pages : 408 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Proteomics for Biological Discovery by : Timothy D. Veenstra

Download or read book Proteomics for Biological Discovery written by Timothy D. Veenstra and published by John Wiley & Sons. This book was released on 2019-05-07 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: An update to the popular guide to proteomics technology applications in biomedical research Building on the strength of the original edition, this book presents the state of the art in the field of proteomics and offers students and scientists new tools and techniques to advance their own research. Written by leading experts in the field, it provides readers with an understanding of new and emerging directions for proteomics research and applications. Proteomics for Biological Discovery begins by discussing the emergence of proteomics technologies and summarizing the potential insights to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are thoroughly covered, from underlying concepts to limitations and future directions. Later chapters provide an overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, applied proteomics, and bioinformatics relevant to proteomics. Chapters cover: Quantitative Proteomics for Differential Protein Expression Profiling; Protein Microarrays; Protein Biomarker Discovery; Biomarker Discovery using Mass Spectrometry Imaging; Protein-Protein Interactions; Mass Spectrometry Of Intact Protein Complexes; Crosslinking Applications in Structural Proteomics; Functional Proteomics; High Resolution Interrogation of Biological Systems via Mass Cytometry; Characterization of Drug-Protein Interactions by Chemoproteomics; Phosphorylation; Large-Scale Phosphoproteomics; and Probing Glycoforms of Individual Proteins Using Antibody-Lectin Sandwich Arrays. Presents a comprehensive and coherent review of the major issues in proteomic technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Features new coverage of mass spectrometry for high throughput proteomic measurements, and novel quantitation strategies such as spectral counting and stable isotope labeling Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Offers new chapters on biomarker discovery, global phosphorylation analysis, proteomic profiling using antibodies, and single cell mass spectrometry Proteomics for Biological Discovery is an excellent advanced resource for graduate students, postdoctoral fellows, and scientists across all the major fields of biomedical science.

TECHNEAU

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Publisher : IWA Publishing
ISBN 13 : 1843392755
Total Pages : 481 pages
Book Rating : 4.8/5 (433 download)

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Book Synopsis TECHNEAU by : Christian Kazner

Download or read book TECHNEAU written by Christian Kazner and published by IWA Publishing. This book was released on 2009-06-14 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The best papers from the three-day conference on Safe Drinking Water from Source to Tap June 2009 in Maastricht are published in this book covering the themes of challenges of the water sector and adaptive strategies, treatment, distribution, risk assessment and risk management, sensors and monitoring, small scale systems, simulation, alternative water supply & sources, consumer involvement, and future drinking water. Worldwide, the water supply sector is facing tremendous challenges. Every new emerging contaminants and pathogens and aging infrastructures that are vulnerable for deliberate contamination pose a threat to the quality of water supplies. Shortage of good quality and readily treatable resources is increasing due to global warming, urbanisation and pollution from agriculture and industry. Regulators and consumers are becoming more demanding. Techneau - the largest European project on drinking water - addresses these challenges by developing adaptive supply system options and new and improved treatment and monitoring technologies. Future system options to be studied are flexible, small scale and multi-source supplies, utilising non conventional resources like brackish ground water, treated wastewater and urban groundwater.

PCR for Clinical Microbiology

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Publisher : Springer Science & Business Media
ISBN 13 : 9048190398
Total Pages : 420 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis PCR for Clinical Microbiology by : Ian W.J. Carter

Download or read book PCR for Clinical Microbiology written by Ian W.J. Carter and published by Springer Science & Business Media. This book was released on 2010-07-03 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology

Soil pollution: a hidden reality

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Publisher : Food & Agriculture Org.
ISBN 13 : 9251305056
Total Pages : 156 pages
Book Rating : 4.2/5 (513 download)

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Book Synopsis Soil pollution: a hidden reality by : Food and Agriculture Organization of the United Nations

Download or read book Soil pollution: a hidden reality written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2018-04-30 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: This document presents key messages and the state-of-the-art of soil pollution, its implications on food safety and human health. It aims to set the basis for further discussion during the forthcoming Global Symposium on Soil Pollution (GSOP18), to be held at FAO HQ from May 2nd to 4th 2018. The publication has been reviewed by the Intergovernmental Technical Panel on Soil (ITPS) and contributing authors. It addresses scientific evidences on soil pollution and highlights the need to assess the extent of soil pollution globally in order to achieve food safety and sustainable development. This is linked to FAO’s strategic objectives, especially SO1, SO2, SO4 and SO5 because of the crucial role of soils to ensure effective nutrient cycling to produce nutritious and safe food, reduce atmospheric CO2 and N2O concentrations and thus mitigate climate change, develop sustainable soil management practices that enhance agricultural resilience to extreme climate events by reducing soil degradation processes. This document will be a reference material for those interested in learning more about sources and effects of soil pollution.

Biosensors for Environmental Monitoring

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Publisher : BoD – Books on Demand
ISBN 13 : 1789238234
Total Pages : 206 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Biosensors for Environmental Monitoring by : Toonika Rinken

Download or read book Biosensors for Environmental Monitoring written by Toonika Rinken and published by BoD – Books on Demand. This book was released on 2019-11-20 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Real-time and reliable detection of molecular compounds and bacteria is essential in modern environmental monitoring. For rapid analyses, biosensing devices combining high selectivity of biomolecular recognition and sensitivity of modern signal-detection technologies offer a promising platform. Biosensors allow rapid on-site detection of pollutants and provide potential for better understanding of the environmental processes, including the fate and transport of contaminants.This book, including 12 chapters from 37 authors, introduces different biosensor-based technologies applied for environmental analyses.

Modern Clinical Molecular Techniques

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Publisher : Springer Science & Business Media
ISBN 13 : 1461421705
Total Pages : 428 pages
Book Rating : 4.4/5 (614 download)

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Book Synopsis Modern Clinical Molecular Techniques by : Peter Hu

Download or read book Modern Clinical Molecular Techniques written by Peter Hu and published by Springer Science & Business Media. This book was released on 2012-05-11 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Quantitative Microbial Risk Assessment

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Publisher : John Wiley & Sons
ISBN 13 : 1118910028
Total Pages : 439 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Quantitative Microbial Risk Assessment by : Charles N. Haas

Download or read book Quantitative Microbial Risk Assessment written by Charles N. Haas and published by John Wiley & Sons. This book was released on 2014-06-09 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the latest QMRA methodologies to determine infection risk cause by either accidental microbial infections or deliberate infections caused by terrorism • Reviews the latest methodologies to quantify at every step of the microbial exposure pathways, from the first release of a pathogen to the actual human infection • Provides techniques on how to gather information, on how each microorganism moves through the environment, how to determine their survival rates on various media, and how people are exposed to the microorganism • Explains how QMRA can be used as a tool to measure the impact of interventions and identify the best policies and practices to protect public health and safety • Includes new information on genetic methods • Techniques use to develop risk models for drinking water, groundwater, recreational water, food and pathogens in the indoor environment