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Pcr For Clinical Microbiology
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Book Synopsis PCR for Clinical Microbiology by : Ian W.J. Carter
Download or read book PCR for Clinical Microbiology written by Ian W.J. Carter and published by Springer Science & Business Media. This book was released on 2010-07-03 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology
Book Synopsis Rapid Cycle Real-Time PCR — Methods and Applications by : U. Reischl
Download or read book Rapid Cycle Real-Time PCR — Methods and Applications written by U. Reischl and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.
Book Synopsis DNA Methods in Clinical Microbiology by : P. Singleton
Download or read book DNA Methods in Clinical Microbiology written by P. Singleton and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology. The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens. Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.
Book Synopsis Clinical Microbilogy by : Charles W Stratton, MD
Download or read book Clinical Microbilogy written by Charles W Stratton, MD and published by Demos Medical Publishing. This book was released on 2011-07-10 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: About the Diagnostic Standards of Care Series A key issue for every laboratory and individual laboratory practitioner is the assessment of risk and a current working knowledge of the standards of care established for diagnostic testing via guidelines, major studies and trials. The Diagnostic Standards of Care series presents an overview of the key diagnoses in clinical pathology using case examples to illustrate effective analysis of the case in light of current evidence and standards for the problem discussed. In addition to being practical diagnostic guides, these volumes will have a unique emphasis on quality assurance and evidence-based testing practices and the role of the pathologist in ensuring quality and patient safety. Clinical Microbiology addresses common medical errors seen in the clinical microbiology laboratory in order to show these errors to pathologists and laboratory technicians as well as clinicians. The goal is to allow such errors to be corrected by both individual effort and a systems approach in the laboratory. The book addresses potential medical errors in test ordering and specimen collection, testing in the laboratory, and reporting and interpretation of test results. Each of these phases can have an adverse impact on the diagnosis and treatment of an infectious disease if a medical error occurs. Potential medical errors are described and discussed in a clinical case-based learning format to effectively illustrate the conditions that contribute to these errors and enable the reader to recognize and avoid them in daily practice. Clinical Microbiology Features Descriptions of potential errors in test ordering and specimen collection in clinical microbiology Descriptions of potential errors in test performance in clinical microbiology Descriptions of potential errors in test reporting and interpretation in clinical microbiology Clinical case discussions provide "real world" illustrations of potential errors and how to anticipate and avoid them in practice Is pocket-sized for portability
Book Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang
Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer Science & Business Media. This book was released on 2007-01-16 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.
Book Synopsis Clinical Microbiology for Diagnostic Laboratory Scientists by : Sarah J. Pitt
Download or read book Clinical Microbiology for Diagnostic Laboratory Scientists written by Sarah J. Pitt and published by John Wiley & Sons. This book was released on 2018-01-16 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: A modern, evaluative, and integrative approach to diagnostic microbiology encouraging problem-solving in the clinical laboratory context through the use of examples to illustrate clinical and diagnostic issues Clinical Microbiology for Diagnostic Laboratory Scientists is designed to encourage readers to develop a way of thinking that can be applied to any diagnostic scenario in microbiology. Through consideration of a selected range of infections caused by pathogenic bacteria, viruses, fungi, protozoa, and helminths, the book encourages readers to explore connections between the available information about clinical symptoms, pathogenesis of infections, and the approaches used in laboratory diagnosis, in order to develop new insights. The book begins with an introductory chapter that outlines the scope of clinical diagnostic microbiology and the key areas for the laboratory scientist to be aware of. The subsequent six chapters review a type of infection in depth, using particular pathogenic microorganisms to illustrate salient points. At the end of each chapter there are three exercises related to management of a diagnostic service and assessing the suitability of test methods to specific contexts. There are no right or wrong answers to these, but the reader can discuss them with their laboratory colleagues or university tutor. Makes extensive use of published research in the form of journal articles, publically available epidemiological data, professional guidelines, and specialist websites Stimulates the reader in critical appraisal of published evidence and encourages problem-solving in the laboratory Outlines the scope of clinical diagnostic microbiology and the key areas for the laboratory scientist to be aware of Considers topics relevant to professional scientists working in the area of diagnostic microbiology Clinical Microbiology for Diagnostic Laboratory Scientists is ideal for post graduate scientists intending to pursue careers in diagnostic clinical microbiology and for biomedical scientists, clinical scientists, and full time students studying for upper level qualifications in biomedical science, microbiology, or virology.
Download or read book Real-time PCR in Microbiology written by and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. A recommended book for all microbiology laboratories.
Book Synopsis Real-time PCR in Microbiology by : Ian Maxwell Mackay
Download or read book Real-time PCR in Microbiology written by Ian Maxwell Mackay and published by Caister Academic Press. This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardisation, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion providing an insightful, topical and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. It is a recommended book for all microbiology laboratories.
Book Synopsis PCR Detection of Microbial Pathogens by : Mark Wilks
Download or read book PCR Detection of Microbial Pathogens written by Mark Wilks and published by Humana Press. This book was released on 2016-08-23 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.
Book Synopsis Clinical Microbiology Procedures Handbook by :
Download or read book Clinical Microbiology Procedures Handbook written by and published by John Wiley & Sons. This book was released on 2020-08-06 with total page 2954 pages. Available in PDF, EPUB and Kindle. Book excerpt: In response to the ever-changing needs and responsibilities of the clinical microbiology field, Clinical Microbiology Procedures Handbook, Fourth Edition has been extensively reviewed and updated to present the most prominent procedures in use today. The Clinical Microbiology Procedures Handbook provides step-by-step protocols and descriptions that allow clinical microbiologists and laboratory staff personnel to confidently and accurately perform all analyses, including appropriate quality control recommendations, from the receipt of the specimen through processing, testing, interpretation, presentation of the final report, and subsequent consultation.
Book Synopsis Rapid Cycle Real-Time PCR — Methods and Applications by : Udo Reischl
Download or read book Rapid Cycle Real-Time PCR — Methods and Applications written by Udo Reischl and published by Springer. This book was released on 2012-12-12 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.
Book Synopsis Practical Medical Microbiology for Clinicians by : Frank E. Berkowitz
Download or read book Practical Medical Microbiology for Clinicians written by Frank E. Berkowitz and published by John Wiley & Sons. This book was released on 2016-02-15 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Infectious diseases constitute a major portion of illnesses worldwide, and microbiology is a main pillar of clinical infectious disease practice. Knowledge of viruses, bacteria, fungi, and parasites is integral to practice in clinical infectious disease. Practical Medical Microbiology is an invaluable reference for medical microbiology instructors. Drs. Berkowitz and Jerris are experienced teachers in the fields of infectious diseases and microbiology respectively, and provide expert insight into microorganisms that affect patients, how organisms are related to each other, and how they are isolated and identified in the microbiology laboratory. The text also is designed to provide clinicians the knowledge they need to facilitate communication with the microbiologist in their laboratory. The text takes a systematic approach to medical microbiology, describing taxonomy of human pathogens and consideration of organisms within specific taxonomic groups. The text tackles main clinical infections caused by different organisms, and supplements these descriptions with clinical case studies, in order to demonstrate the effects of various organisms. Practical Medical Microbiology is an invaluable resource for students, teachers, and researchers studying clinical microbiology, medical microbiology, infectious diseases, and virology.
Author :James Versalovic Publisher :American Society for Microbiology Press ISBN 13 :1555814638 Total Pages :3194 pages Book Rating :4.5/5 (558 download)
Book Synopsis Manual of Clinical Microbiology by : James Versalovic
Download or read book Manual of Clinical Microbiology written by James Versalovic and published by American Society for Microbiology Press. This book was released on 2011-05-16 with total page 3194 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most authoritative, comprehensive reference in the field. • Sets the standard for state-of-the-science laboratory practice. • A collaborative effort of 22 editors and more than 260 authors from around the world, all experienced researchers and practitioners in medical and diagnostic microbiology. • Includes 149 chapters of the latest research findings, infectious agents, methods, practices, and safety guidelines. • Indispensable to clinical microbiologists, laboratory technologists, and infectious disease specialists in hospitals, clinics, reference laboratories, and more
Book Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang
Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer. This book was released on 2018-11-09 with total page 760 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.
Book Synopsis Current and Emerging Technologies for the Diagnosis of Microbial Infections by :
Download or read book Current and Emerging Technologies for the Diagnosis of Microbial Infections written by and published by Academic Press. This book was released on 2015-11-23 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current and Emerging Technologies in Microbial Diagnostics, the latest volume in the Methods in Microbiology series, provides comprehensive, cutting-edge reviews of current and emerging technologies in the field of clinical microbiology. The book features a wide variety of state-of-the art methods and techniques for the diagnosis and management of microbial infections, with chapters authored by internationally renowned experts. This volume focuses on current techniques, such as MALDI-TOF mass spectroscopy and molecular diagnostics, along with newly emerging technologies such as host-based diagnostics and next generation sequencing. Written by recognized leaders and experts in the field Provides a comprehensive and cutting-edge review of current and emerging technologies in the field of clinical microbiology, including discussions of current techniques such as MALDI-TOF mass spectroscopy and molecular diagnostics Includes a broad range and breadth of techniques covered Presents discussions on newly emerging technologies such as host-based diagnostics and next generation sequencing
Book Synopsis Molecular Microbiology by : David H. Persing
Download or read book Molecular Microbiology written by David H. Persing and published by John Wiley & Sons. This book was released on 2020-07-24 with total page 835 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.
Book Synopsis Advanced Techniques in Diagnostic Microbiology by : Yi-Wei Tang
Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer. This book was released on 2018-11-09 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.
