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Control Of Translation Initiation In Escherichia Coli Following Nutrient Downshift Or Infection By The Bacteriophage T7
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Book Synopsis Control of Translation Initiation in Escherichia Coli Following Nutrient Downshift Or Infection by the Bacteriophage T7 by : Roger Sherman Provost
Download or read book Control of Translation Initiation in Escherichia Coli Following Nutrient Downshift Or Infection by the Bacteriophage T7 written by Roger Sherman Provost and published by . This book was released on 1995 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Expression written by Lynn Cloney and published by . This book was released on 1988 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Regulation of Gene Expression in Escherichia coli by : E. C. C. Lin
Download or read book Regulation of Gene Expression in Escherichia coli written by E. C. C. Lin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.
Book Synopsis Effect of Zero-valent Iron on the Removal of Escherichia Coli O157:H7 from Water by : Alexandra Derevianko
Download or read book Effect of Zero-valent Iron on the Removal of Escherichia Coli O157:H7 from Water written by Alexandra Derevianko and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, outbreaks of Escherichia coli O157:H7 have been increasingly associated with leafy green vegetables. The ability of producers to deliver a fresh, safe, ready-to-eat product has come under intense scrutiny. Numerous studies have shown that current commercial sanitizers used at U.S. Food and Drug Administration-approved concentrations can only achieve a 1- to 2-log reduction in microbial populations and may also create by-products that are harmful to humans and the environment. In September 2006, one of the largest recorded outbreaks of E. coli O157:H7 occurred involving pre-bagged spinach grown in the Salinas Valley in California. Contaminated water was thought to be a possible source of contamination in this outbreak. Escherichia coli O157:H7 has been shown to survive for long periods in water and is known to be passed from the manure of infected animals such as cattle or pigs into lakes, rivers, and groundwater through runoff and leaching. For many years, Zero-valent iron (ZVI) has been used for remediation of groundwater contaminated with chlorinated compounds, especially trichloroethene (TCE) and lesser amounts of degradation by-products such as dichloroethene (DCE). ZVI has also recently been shown to be successful in removing viruses from water, while nanoparticles of ZVI have been investigated and have demonstrated some positive results in the inactivation of generic E. coli strains. A novel water filtration system using ZVI was investigated as a simple and inexpensive approach to reducing E. coli O157:H7 in water for both pre-and post-harvest processes. This study was initiated to determine the effectiveness of ZVI in the removal and inactivation of E. coli O157:H7 from water used for washing fresh-cut spinach. Breakthrough curves at pH values of 5.0, 7.5, and 9.0 showed reduction of 1-2 log cfu/ml of E. coli O157:H7 by the inclusion of ZVI compared to sand. After dissecting and investigating the columns, bacterial survival was observed to be 2.38 +- 0.67 log cfu/ml less in ZVI than in the sand column at identical locations. These experiments were performed with feshly-packed columns, aged one month or less. Mature columns (aged> 1 month) were found to be more effective in lowering survival of E. coli O157:H7 in water by>= 5 log cfu/ml, and were used in the following experiments. Dip and spot inoculations were performed using inoculated, ZVI-treated water on spinach. The ZVI-tread E. coli O157:H7 showed almost no survival as compared to E. coli O157:H7 recovered from spinach dipped in or spot inoculated with sand column-treated water. Levels of E. coli O157:H7 recovered from treated spinach leaves went from 3.59 +- 0.27 log cfu/ml initial concentration down to 0.25 " 0.50 log cfu/ml and 3.69 " 1.98 log cfu/ml initial concentration down to 0.23 +- 0.40 log cfu/ml for each method, respectively. As determined with a vero toxin assay, toxin was produced by ZVI-treated E. coli O157:H7 only after an enrichment and recovery period. ZVI-treated cells from mature columns were shown to produce less toxin than control cells. Storage tests showed survival of ZVI-treated E. coli O157:H7 for 7 days with cells detected only after enrichment. ZVI is a simple, inexpensive, yet highly effective technology that has been shown to reduce E. coli O157:H7 in water. It has great potential for reducing water contamination and can be used to reduce or eliminate the risk of E. coli O157:H7 on produce such as lettuce, spinach, and other leafy greens which have been implicated in past outbreaks. The use of this technology has many far reaching applications including, but by no means limited to, decontaminating irrigation and wash water used for fresh produce.
Book Synopsis E. coli Plasmid Vectors by : Nicola Casali
Download or read book E. coli Plasmid Vectors written by Nicola Casali and published by Humana Press. This book was released on 2010-11-10 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Book Synopsis Biology and Biotechnology of Actinobacteria by : Joachim Wink
Download or read book Biology and Biotechnology of Actinobacteria written by Joachim Wink and published by Springer. This book was released on 2017-10-19 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides in-depth insights into the biology, taxonomy, genetics, physiology and biotechnological applications of Actinobacteria. It especially focuses on the latter, reviewing the wide variety of actinobacterial bioactive molecules and their benefits for diverse industrial applications such as agriculture, aquaculture, biofuel production and food technology. Actinobacteria are one of the most promising sources of small bioactive molecules and it is estimated that only a small percentage of actinobacterial bioactive chemicals have been discovered to date. Identifying new diverse gene clusters of biotechnological relevance in the genome of Actinobacteria will be crucial to developing advanced applications for pharmaceutical, industrial and agricultural purposes. The book offers a unique resource for all graduate students, researchers and practitioners in the fields of microbiology, microbial biotechnology, and the genetic engineering of Actinobacteria.
Download or read book Zebrafish written by James F. Amatruda and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.
Book Synopsis Nucleic Acids by : Marcelo Larramendy
Download or read book Nucleic Acids written by Marcelo Larramendy and published by BoD – Books on Demand. This book was released on 2016-03-16 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.
Book Synopsis Control of Messenger RNA Stability by : Joel Belasco
Download or read book Control of Messenger RNA Stability written by Joel Belasco and published by Academic Press. This book was released on 1993-04-06 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first comprehensive review of mRNA stability and its implications for regulation of gene expression. Written by experts in the field, Control of Messenger RNA Stability serves both as a reference for specialists in regulation of mRNA stability and as a general introduction for a broader community of scientists. Provides perspectives from both prokaryotic and eukaryotic systems Offers a timely, comprehensive review of mRNA degradation, its regulation, and its significance in the control of gene expression Discusses the mechanisms, RNA structural determinants, and cellular factors that control mRNA degradation Evaluates experimental procedures for studying mRNA degradation
Book Synopsis Minimal Cells: Design, Construction, Biotechnological Applications by : Alvaro R. Lara
Download or read book Minimal Cells: Design, Construction, Biotechnological Applications written by Alvaro R. Lara and published by Springer Nature. This book was released on 2019-12-04 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the design, generation and characterization of minimal cell systems. Written by leading experts, it presents an in-depth analysis of the current issues and challenges in the field, including recent advances in the generation and characterization of reduced-genome strains generated from model organisms with relevance in biotechnology, and basic research such as Escherichia coli, Corynebacterium glutamicum and yeast. It also discusses methodologies, such as bottom-up and top-down genome minimization strategies, as well as novel analytical and experimental approaches to characterize and generate minimal cells. Lastly, it presents the latest research related to minimal cells of serveral microorganisms, e.g. Bacillus subtilis. The design of biological systems for biotechnological purposes employs strategies aimed at optimizing specific tasks. This approach is based on enhancing certain biological functions while reducing other capacities that are not required or that could be detrimental to the desired objective. A highly optimized cell factory would be expected to have only the capacity for reproduction and for performing the expected task. Such a hypothetical organism would be considered a minimal cell. At present, numerous research groups in academia and industry are exploring the theoretical and practical implications of constructing and using minimal cells and are providing valuable fundamental insights into the characteristics of minimal genomes, leading to an understanding of the essential gene set. In addition, research in this field is providing valuable information on the physiology of minimal cells and their utilization as a biological chassis to which useful biotechnological functions can be added.
Book Synopsis The Biology of Vibrios by : Fabiano Lopes Thompson
Download or read book The Biology of Vibrios written by Fabiano Lopes Thompson and published by . This book was released on 2006 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Biology of Halophilic Bacteria by : Russell H. Vreeland
Download or read book The Biology of Halophilic Bacteria written by Russell H. Vreeland and published by CRC Press. This book was released on 1992-11-30 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: A book for anyone interested in halophilic bacteria The Biology of Halophilic Bacteria presents detailed information regarding methods for working with halophilic bacteria. Helpful hints for performing various tests and assays in high salts are given, and information about data presentation and analysis is provided as well. The book will be useful to molecular biologists, biochemists, ecologists, and others interested in halophilic bacteria.
Book Synopsis High-Throughput Phenotyping in Plants by : Jennifer Normanly
Download or read book High-Throughput Phenotyping in Plants written by Jennifer Normanly and published by Humana Press. This book was released on 2017-04-30 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.
Book Synopsis Cell-Free Gene Expression by : Ashty S. Karim
Download or read book Cell-Free Gene Expression written by Ashty S. Karim and published by . This book was released on 2022 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.
Book Synopsis Control of Macromolecular Synthesis by : Ole Maaløe
Download or read book Control of Macromolecular Synthesis written by Ole Maaløe and published by . This book was released on 1966 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The GMO Handbook by : Sarad R. Parekh
Download or read book The GMO Handbook written by Sarad R. Parekh and published by Springer Science & Business Media. This book was released on 2004-05-15 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive and accessible survey of the best current accomplishments of GMO research in all their complexity and ramifications. The authors introduce the fundamentals of biotechnology as a scientific discipline, show how GMO research is conducted today, discuss the problems that have arisen from genetic technology and the tools needed to resolve them, and describes how GMO-derived technology may impact our lives in the future. On the technical side, the authors examine a wide range of current technologies employed for constructing GMOs, and describe approaches to novel research, appropriate protocols, and the process of constructing and screening a GMO. The discussion of plant and animal cells covers new strategies employed and the large-scale expression and purification of recombinant products in cultured cells. Social political, and legal issues are also discussed.
Book Synopsis PCR Detection of Microbial Pathogens by : Konrad Sachse
Download or read book PCR Detection of Microbial Pathogens written by Konrad Sachse and published by Springer Science & Business Media. This book was released on 2003 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.
