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Applied Rnai
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Download or read book Applied RNAi written by Patrick Arbuthnot and published by Caister Academic Press Limited. This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of RNA interference (RNAi) in 1998, research on the topic has advanced at an impressive pace. Small RNAs and, in particular, micro RNAs (miRNAs) play a fundamental role in gene regulation through the activation of RNAi. Detailed insights into the mechanisms of RNAi have led to an improved understanding of gene regulation in normal and disease states, and thereby enabled the exploitation of RNAi for a variety of applications. In this book, an international panel of RNAi experts critically reviews the most interesting advances in basic applied RNAi research, highlighting the applications in RNAi-based therapies and discussing the technical hurdles that remain. Topics covering the fundamental biological aspects of applied RNAi research include: the role of miRNAs in trinucleotide repeat disorders * miRNAs and HIV pathogenesis * miRNAs for epigenetic gene silencing * the role of miRNAs in virus-related cancers * non-canonical miRNA biogenesis. In the area of RNAi-based therapy, topics include: harnessing RNAi for the treatment of viral infections * optimizing the design of exogenous RNAi activators * blocking miRNA function with synthetic agents * somatic cell reprogramming * high-content miRNA-based screening tools * the use of miRNA target sites for control of transgene expression. The book will be essential reading for everyone involved in RNAi research, drug discovery and delivery, biomedical engineering, and biomaterials. Subject: Molecular Biology, Genomics, Life Science]
Book Synopsis CRISPR and RNAi Systems by : Kamel A Abd-Elsalam
Download or read book CRISPR and RNAi Systems written by Kamel A Abd-Elsalam and published by Elsevier. This book was released on 2021-02-27 with total page 842 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale
Download or read book RNAi written by Gregory J. Hannon and published by CSHL Press. This book was released on 2003 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.
Book Synopsis RNA Technologies and Their Applications by : Volker A. Erdmann
Download or read book RNA Technologies and Their Applications written by Volker A. Erdmann and published by Springer Science & Business Media. This book was released on 2010-09-02 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA technologies are the driving forces of modern medicine and biotechnology. They combine the fields of biochemistry, chemistry, molecular biology, cell biology, physics, nanotechnology and bioinformatics. The combination of these topics is set to revolutionize the medicine of tomorrow. After more than 15 years of extensive research in the field of RNA technologies, the first therapeutics are ready to reach the first patients. Thus we are witnessing the birth of a very exciting time in the development of molecular medicine, which will be based on the methods of RNA technologies. This volume is the first of a series. It covers various aspects of RNA interference and microRNAs, although antisense RNA applications, hammerhead ribozyme structure and function as well as non-coding RNAs are also discussed. The authors are internationally highly respected experts in the field of RNA technologies.
Book Synopsis Advanced Delivery and Therapeutic Applications of RNAi by : Kun Cheng
Download or read book Advanced Delivery and Therapeutic Applications of RNAi written by Kun Cheng and published by John Wiley & Sons. This book was released on 2013-04-19 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt: Commonly used by researchers to develop technologies for modifying and studying genetic process, RNA interference (RNAi) has many potential uses in medicine, biotechnology, and functional genomics. This book covers all essential aspects involved in the development of RNAi therapeutics, providing detailed guidance on the challenges and opportunities of bringing RNAi technologies from bench to clinic. It explores the design and mechanism of RNAi molecules, delivery strategies, and therapeutic applications in various diseases. Preclinical, regulatory, market, and intellectual aspects of RNAi technologies are also covered.
Download or read book RNAi Technology written by R. K. Gaur and published by CRC Press. This book was released on 2016-04-19 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Book Synopsis Gene Silencing by RNA Interference by : Muhammad Sohail
Download or read book Gene Silencing by RNA Interference written by Muhammad Sohail and published by CRC Press. This book was released on 2004-08-27 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet. This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms. From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.
Book Synopsis Therapeutic Applications of RNAi by : John F. Reidhaar-Olson
Download or read book Therapeutic Applications of RNAi written by John F. Reidhaar-Olson and published by Humana Press. This book was released on 2010-07-08 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.
Download or read book RNAi written by Sailen Barik and published by Humana. This book was released on 2011-01-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA interference (RNAi) is the most recent discovery to revolutionize the study of biology. In this book, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Cutting edge and clearly written, this book enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.
Download or read book RNAi written by Martin Latterich and published by Garland Science. This book was released on 2008-09-04 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful research tool in the functional study of individual genes and their products, and in functional genomics. In RNAi, specific small double-stranded RNA molecules (small interfering RNAs or siRNAs) are introduced into cells to selectively silence certain genes. RNAi covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent. A key feature of RNAi is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations.
Book Synopsis Advances and Challenges of RNAi Based Technologies for Plants by : Susana Araújo
Download or read book Advances and Challenges of RNAi Based Technologies for Plants written by Susana Araújo and published by Frontiers Media SA. This book was released on 2021-07-28 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNAi Technologies by : Prerna Pandey
Download or read book RNAi Technologies written by Prerna Pandey and published by Delve Publishing. This book was released on 2017-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA interference (RNAi), one of the most exciting discoveries of the last decade, is also known as Post-Transcriptional Gene Silencing (PTGS). It is a conserved host defense response via double-stranded RNA against both endogenous and foreign nucleic acids, and occurs at the levels of transcription, post-transcription and translation. RNA induced gene silencing has been used, with considerable success as a reverse genetics tool for the study of gene function. This technology has revolutionized the gene expression analysis studies because of the specificity and speed it offers. The small RNA molecules central to RNAi are microRNA (miRNA) and small interfering RNA (siRNA). These molecules bind to other RNAs specifically, such as messenger RNA (mRNA), to regulate their activities.In this book, we will discuss how gene silencing methodology has been successfully applied as therapeutic tool against metabolic disorders, cancer, and infectious diseases. This tool has also been successfully used to improve crops resistance against plant pathogens. In contrast to conventional knock-out mutagenesis techniques, the "gene-silencing" vectors, that allow the study of functions of genes whose complete knock-out would otherwise be lethal to the plant.
Download or read book RNA Interference written by Dr. Tim Doran and published by CABI. This book was released on 2009 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.
Book Synopsis RNA Interference Technology by : Krishnarao Appasani
Download or read book RNA Interference Technology written by Krishnarao Appasani and published by Cambridge University Press. This book was released on 2005-01-17 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
Book Synopsis RNA Interference in Practice by : Ute Schepers
Download or read book RNA Interference in Practice written by Ute Schepers and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
Book Synopsis RNAi Based Pesticides by : Hailing Jin
Download or read book RNAi Based Pesticides written by Hailing Jin and published by Frontiers Media SA. This book was released on 2021-09-29 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012) by : Tong-Cun Zhang
Download or read book Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012) written by Tong-Cun Zhang and published by Springer Science & Business Media. This book was released on 2013-11-26 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 2012 International Conference on Applied Biotechnology (ICAB 2012) was held in Tianjin, China on October 18-19, 2012. It provides not only a platform for domestic and foreign researchers to exchange their ideas and experiences with the application-oriented research of biotechnology, but also an opportunity to promote the development and prosperity of the biotechnology industry. The proceedings of ICAB 2012 mainly focus on the world's latest scientific research and techniques in applied biotechnology, including Industrial Microbial Technology, Food Biotechnology, Pharmaceutical Biotechnology, Environmental Biotechnology, Marine Biotechnology, Agricultural Biotechnology, Biological Materials and Bio-energy Technology, Advances in Biotechnology, and Future Trends in Biotechnology. These proceedings are intended for scientists and researchers engaging in applied biotechnology. Professor Pingkai Ouyang is the President of the Nanjing University of Technology, China. Professor Tongcun Zhang is the Director of the Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education at the College of Bioengineering, Tianjin University of Science and Technology, China. Dr. Samuel Kaplan is a Professor at the Department of Microbiology & Molecular Genetics at the University of Texas at Houston Medical School, Houston, Texas, USA. Dr. Bill Skarnes is a Professor at Wellcome Trust Sanger Institute, United Kingdom.
