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An Introduction To Centrifugation
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Book Synopsis An Introduction to Centrifugation by : T. C. Ford
Download or read book An Introduction to Centrifugation written by T. C. Ford and published by Taylor & Francis. This book was released on 1991 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This introduction explains what centrifugation is, what it does, how it does it, and the types of centrifuges, rotors, techniques, and density-gradient media currently available.
Book Synopsis Biological Centrifugation by : Dr John Graham
Download or read book Biological Centrifugation written by Dr John Graham and published by Garland Science. This book was released on 2020-12-17 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
Book Synopsis Centrifugal Separations in Biotechnology by : Wallace Woon-Fong Leung
Download or read book Centrifugal Separations in Biotechnology written by Wallace Woon-Fong Leung and published by Butterworth-Heinemann. This book was released on 2020-03-13 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugal Separations in Biotechnology, Second Edition, is the only book on the market devoted to centrifugal separation in biotechnology. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. Centrifugal Separations in Biotechnology, Second Edition, provides guidance on troubleshooting and optimizing centrifuges, and then goes on to explore the commercial applications of centrifuges in biotechnology. It gives detailed process information and data to assist in the development of particular processes from existing systems. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. Provides a comprehensive guide to centrifuges, their optimal development, and their operation in the biotechnology industry Updated throughout based on developments in industrial applications and advances in our understanding of centrifugal separations in biotechnology Discusses applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements Includes new sections on use of optimal polymer dosage in waste treatment, new centrifuge designs for applications in algae processing, biopharma, and more
Book Synopsis Centrifugation in Density Gradients by : C. A. Price
Download or read book Centrifugation in Density Gradients written by C. A. Price and published by Academic Press. This book was released on 2014-06-28 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugation in Density Gradients provides information pertinent to the fundamental aspects of density gradient centrifugation. This book discusses the benefits of density gradient centrifugation to membrane-bound particles. Organized into nine chapters, this book begins with an overview of the method of differential or fractional centrifugation. This text then explores the physical basis of density gradient centrifugation. Other chapters deal with the nuts and bolts of density gradient centrifugation, the construction and composition of gradients, the properties and operation of centrifuge systems, and certain arcane but highly useful procedures. This book discusses as well density gradient centrifugation in the analytical ultracentrifuge. The final chapter deals with a collection of protocols for separating particles ranging in size from whole cells to macromolecules. This book is intended to be suitable for readers who need to separate biological particles. Biologists, chemists, biochemists, cytologists, physiologists, scientists, and research workers will also find this book useful.
Book Synopsis Centrifugal Separations in Molecular and Cell Biology by : G.D. Birnie
Download or read book Centrifugal Separations in Molecular and Cell Biology written by G.D. Birnie and published by Butterworth-Heinemann. This book was released on 2014-06-28 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.
Download or read book Centrifugation written by David Rickwood and published by . This book was released on 1984 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition provides extensive experimental details of protocols for all types of centrifugal separations from macrmolecules to whole cells. It also describes the applications of centrifuges ranging from simle bench machines to analytical centrifuges.
Book Synopsis Biological Centrifugation by : John Graham
Download or read book Biological Centrifugation written by John Graham and published by Turtleback. This book was released on 2001-01-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is both an important introduction to the use of the centrifuge in the biological laboratory, and a useful guide for more experienced researchers. It describes the background and principles behind centrifugation, including sedimentation theory. It also considers the different types of centrifuges and centrifuge hardware available, density gradient media and gradient technology.
Book Synopsis Biological Centrifugation by : Dr John Graham
Download or read book Biological Centrifugation written by Dr John Graham and published by Garland Science. This book was released on 2020-12-18 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
Book Synopsis Ultracentrifugation in Biochemistry by : Howard K. Schachman
Download or read book Ultracentrifugation in Biochemistry written by Howard K. Schachman and published by Elsevier. This book was released on 2013-10-22 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultracentrifugation in Biochemistry discusses the fundamental aspects of ultracentrifugation. The book begins with a sketch of the field, highlighting some of the principal developments. Following this is a chapter that discusses ultracentrifugation in general terms and describes the division of the field into three major areas. The subsequent chapter deals with developments of the experimental aspects of the field such as improvements in the instrument itself, cells, rotors, measurement, and control of temperature, and the various optical systems. The remainder of the book discusses the fundamental principles of sedimentation velocity, transient states, and sedimentation equilibrium. A section is also included which deals with interpretation of sedimentation data in terms of hydrodynamic models, charge effects, and interactions in multicomponent systems. This book is likely to become an indispensable companion to the laboratory worker who is planning and conducting an ultracentrifuge run for almost any purpose. It should also be of fundamental value to the thoughtful student or investigator who wants to know the present state of knowledge in the field, both experimental and theoretical.
Book Synopsis Principles and Techniques of Biochemistry and Molecular Biology by : Keith Wilson
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Book Synopsis An Introduction to Ultracentrifugation by : Thomas John Bowen
Download or read book An Introduction to Ultracentrifugation written by Thomas John Bowen and published by John Wiley & Sons. This book was released on 1970 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biological Centrifugation by : J. M. Graham
Download or read book Biological Centrifugation written by J. M. Graham and published by Taylor & Francis. This book was released on 1999-04 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
Book Synopsis Flow Cytometry by : Alice Longobardi Givan
Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Book Synopsis Platelet Rich Fibrin in Regenerative Dentistry by : Richard J. Miron
Download or read book Platelet Rich Fibrin in Regenerative Dentistry written by Richard J. Miron and published by John Wiley & Sons. This book was released on 2017-08-18 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book devoted exclusively to the subject, Platelet Rich Fibrin in Regenerative Dentistry offers comprehensive, evidence-based coverage of the biological basis and clinical applications of PRF in dentistry. Co-edited by a leading researcher in tissue regeneration and the inventor of the PRF technique, it brings together original contributions from expert international researchers and clinicians. Chapters cover the biological foundation of PRF before addressing specific uses of the technology within clinical dentistry. Topics describe the use of PRF in many dental applications, including extraction socket management, sinus lifting procedures, root coverage, periodontal regeneration, soft tissue healing around implants, guided bone regeneration, and facial esthetics. The text is supplemented with color photographs and explanatory illustrations throughout. Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications is an indispensable professional resource for periodontists, oral surgeons and oral and maxillofacial surgeons, as well as general dentists who use PRF or are interested in introducing it into their practices. It is also an excellent reference for undergraduate and postgraduate dental students.
Book Synopsis Industrial Centrifugation Technology by : Wallace Woon-Fong Leung
Download or read book Industrial Centrifugation Technology written by Wallace Woon-Fong Leung and published by McGraw Hill Professional. This book was released on 1998 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction General Principles of Sedimenting Centrifuges Batch Sedimenting Centrifuges Continous Sedimenting Centrifuges Applications of Sedimenting Centrifuges Continous-Feed Sedimentation General Principles of Filtering Centrifuges Batch Filtering Centrifuges Continuous Filtering Centrifuges Applications of Filtering Centrifuges Feed Acceleration Lab, Pilot, and Production Tests Centrifuge Selection and Sizing Optimization and Troubleshooting Kaolin Processing Dewatering of Compactible Solids Cake compaction theory Appendices Name Index Subject Index.
Book Synopsis Centrifugation Techniques by : Shalinee Naidoo
Download or read book Centrifugation Techniques written by Shalinee Naidoo and published by Arcler Press. This book was released on 2017-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology involves the analysis of cells and tissues at both the morphological and biochemical levels in an attempt to understand the molecular mechanisms operating in any living organism. Proper biochemical analysis of biological samples is imperative for constructing complete and true cell biology. The subcellular organization of cells can be studied via electron microscopy or by the separation and subsequent molecular and biochemical analysis of individual cellular components. While electron microscopy provides detailed information about the appearance of cellular organelles, not much information can be obtained regarding their individual functions. As such, a way to separate the different organelles from remaining cellular components was needed (OpenLearn, 2017). This was made possible by a technique known as cell fractionation which allows for the isolation of various cellular components (nuclear, cytosolic and mitochondrial) based on their subcellular compartmentalized distribution followed by analysis of their molecular information ranging from the state and distribution of a cell or subcellular component to their response to a range of treatments (Dimauro et al, 2012). The separation of cellular components and particles by sedimentation is one of the most powerful tools in biology. Even though sedimentation using centrifugation is not a new technology, it is essential for conducting cutting edge genomic and proteomic research, as it aids in providing purified particles of interest. As such, centrifugation forms one of the most important and widely applied research techniques used in various disciplines today. It services a range of research fields such as biochemistry, cellular and molecular biology while allowing large scale applications across a broad spectrum of industries ranging from large scale commercial applications in the pharmaceutical and agricultural industries to small scale laboratory-scale scientific studies. Miescher was one of the first researchers to use a centrifuge for the isolation of a cell organelle when he separated nuclei from human pus cells in 1871. A survey conducted at the US National Institutes of Health revealed that over 65% of research workers believe that using centrifugation to purify cells, subcellular organelles, viruses, proteins, and nucleic acids is an essential part of their everyday research, further proving the importance of this technique in today's scientific world (Sigma-Aldrich, 2011). Current research applications rely on centrifugation for the isolation and harvest of cells, sub cellular components and macromolecules in high yields. It is commonly used as a separation technique when gravity separation is too slow and particles within the sample to be separated do not settle readily or at all. This technique has since paved the way for the determination and understanding of fundamental cellular processes such as oxidative phosphorylation, intracellular digestion, protein synthesis as well as transportation of substances in and out of the cell (Graham and Rickwood). Thus centrifuges are now regarded as one of the most basic yet valuable pieces of equipment required in biological laboratories. This book aims to provide an introduction into the history and principles of centrifugation as well as an in depth explanation of the history and principles surrounding centrifugation. It also aims to provide an explanation of commonly used centrifugation techniques and their related practical applications in various industries. Furthermore, to aid in understanding the various principles behind this technique, the basic design of various rotors have been diagrammatically presented. Also in this book is an overview of the currently available centrifuge types and related instrument components as well as a description of centrifugal theory, a table comparing densities of commonly separated macromolecules and additional information on separation techniques together with a comprehensive advice for proper centrifuge handling, maintenance and related safety protocols. There is also further information on sample preparation and and how to design an ideal centrifugation protocol.
Download or read book Food Analysis written by Y. Pomeranz and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 788 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Food Analysis: Theory and Practice was published in 1971 and was revised in 1978. The second edition was published in 1987, and in 1993 we found it necessary to prepare a third edition to reflect and cover the most recent advances in the field of food analysis. A complete revision of a book is an arduous and anguished task. The following are challenges that we wanted to address in this revision: to update the material without eliminating classic and time-preserved and honored methods used by the food analyst; to broaden and deepen the coverage and scope without increasing the size of the book; and to produce a textbook (for senior undergraduate and graduate students) with regard to objectives, scope, and outlay while providing a reference and resource for the worker and researcher in the field of food analysis. To meet those challenges we added much new material and took out practically the same amount of "rel atively outdated" material. Every chapter has been extensively updated and revised; many of the pictures in the previous editions were deleted and, whenever available and appropriate, were replaced by diagrams or flow sheets. In Part I we have expanded the seetions on sampling, preparation of sam pIes, reporting results, and reliability of analyses.
