Agrobacterium Biology And Its Application To Transgenic Plant Production

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Agrobacterium biology and its application to transgenic plant production

Author : Hau-Hsuan Hwang
Publisher : Frontiers Media SA
ISBN 13 : 2889195740
Total Pages : 167 pages
Book Rating : 4.8/5 (891 download)

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Book Synopsis Agrobacterium biology and its application to transgenic plant production by : Hau-Hsuan Hwang

Download or read book Agrobacterium biology and its application to transgenic plant production written by Hau-Hsuan Hwang and published by Frontiers Media SA. This book was released on 2015-06-26 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Agrobacterium: From Biology to Biotechnology

Author : Tzvi Tzfira
Publisher : Springer Science & Business Media
ISBN 13 : 0387722904
Total Pages : 768 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Agrobacterium: From Biology to Biotechnology by : Tzvi Tzfira

Download or read book Agrobacterium: From Biology to Biotechnology written by Tzvi Tzfira and published by Springer Science & Business Media. This book was released on 2007-12-25 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.

Transgenic Plants

Author : Leandro Peña
Publisher : Springer Science & Business Media
ISBN 13 : 1592598277
Total Pages : 427 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Transgenic Plants by : Leandro Peña

Download or read book Transgenic Plants written by Leandro Peña and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.

Transgenic Crops of the World

Author : Ian S. Curtis
Publisher : Springer Science & Business Media
ISBN 13 : 1402023332
Total Pages : 448 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Transgenic Crops of the World by : Ian S. Curtis

Download or read book Transgenic Crops of the World written by Ian S. Curtis and published by Springer Science & Business Media. This book was released on 2012-08-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

Plant Biotechnology and Genetics

Author : C. Neal Stewart, Jr.
Publisher : John Wiley & Sons
ISBN 13 : 1118589483
Total Pages : 554 pages
Book Rating : 4.1/5 (185 download)

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Book Synopsis Plant Biotechnology and Genetics by : C. Neal Stewart, Jr.

Download or read book Plant Biotechnology and Genetics written by C. Neal Stewart, Jr. and published by John Wiley & Sons. This book was released on 2012-12-13 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.

Advances in Plant Transgenics: Methods and Applications

Author : Ramalingam Sathishkumar
Publisher : Springer Nature
ISBN 13 : 9811396248
Total Pages : 374 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Advances in Plant Transgenics: Methods and Applications by : Ramalingam Sathishkumar

Download or read book Advances in Plant Transgenics: Methods and Applications written by Ramalingam Sathishkumar and published by Springer Nature. This book was released on 2019-11-15 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The green revolution led to the development of improved varieties of crops, especially cereals, and since then, classical or molecular breeding has resulted in the creation of economically valuable species. Thanks to recent developments in genetic engineering, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. This technology has made the scientific community aware of the critical role of transgenics, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. This book discusses the commercial applications of plant transgenic technologies, including the use of transgenic cell culture approachesto improve the production of metabolites and high-value therapeutics as well as transgenic plants in pest management. It also explores generation of novel vectors, protein production using chloroplast engineering and the latest developments in this area, such as genome editing in plants. Featuring general discussions and research papers by leading international experts, it is a valuable resource for scientists, teachers, students and industrialists working in the field.

Transgenic Crop Plants

Author : Chittaranjan Kole
Publisher : Springer Science & Business Media
ISBN 13 : 3642048099
Total Pages : 807 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis Transgenic Crop Plants by : Chittaranjan Kole

Download or read book Transgenic Crop Plants written by Chittaranjan Kole and published by Springer Science & Business Media. This book was released on 2010-01-13 with total page 807 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 1 with ten chapters contributed by 31 eminent scientists from nine countries deliberates on the basic concepts, strategies and tools for development of transgenic crop plants, including topics such as: explants used for the generation of transgenic plants, gene transfer methods, organelle transformation, selection and screening strategies, expression and stability of transgenes, silencing undesirable genes, transgene integration, biosynthesis and biotransformation and metabolic engineering of pathways and gene discovery.

Genetic Transformation

Author : María Alvarez
Publisher : BoD – Books on Demand
ISBN 13 : 9533073640
Total Pages : 344 pages
Book Rating : 4.5/5 (33 download)

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Book Synopsis Genetic Transformation by : María Alvarez

Download or read book Genetic Transformation written by María Alvarez and published by BoD – Books on Demand. This book was released on 2011-09-06 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic transformation of plants has revolutionized both basic and applied plant research. Plant molecular biology and physiology benefit from this power fool, as well as biotechnology. This book is a review of some of the most significant achievements that plant transformation has brought to the fields of Agrobacterium biology, crop improvement and, flower, fruit and tree amelioration. Also, it examines their impact on molecular farming, phytoremediation and RNAi tools.

Transgenic Plants

Author :
Publisher : Academic Press
ISBN 13 : 0323140262
Total Pages : 300 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Transgenic Plants by :

Download or read book Transgenic Plants written by and published by Academic Press. This book was released on 2012-12-02 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.

Transgenic Plants

Author :
Publisher : Academic Press
ISBN 13 : 0323140254
Total Pages : 403 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Transgenic Plants by :

Download or read book Transgenic Plants written by and published by Academic Press. This book was released on 2012-12-02 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.

Gene Transfer to Plants

Author : Ingo Potrykus
Publisher : Springer Science & Business Media
ISBN 13 : 3642792472
Total Pages : 370 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Gene Transfer to Plants by : Ingo Potrykus

Download or read book Gene Transfer to Plants written by Ingo Potrykus and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nuclear Import and Export in Plants and Animals

Author : Tzvi Tzfira
Publisher : Springer Science & Business Media
ISBN 13 : 0387277471
Total Pages : 239 pages
Book Rating : 4.3/5 (872 download)

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Book Synopsis Nuclear Import and Export in Plants and Animals by : Tzvi Tzfira

Download or read book Nuclear Import and Export in Plants and Animals written by Tzvi Tzfira and published by Springer Science & Business Media. This book was released on 2007-02-25 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Import and Export in Plants and Animals provides insight into the remarkable mechanisms of nuclear import and export. This book covers a range of topics from the nuclear pore structure, to nuclear import and export of macromolecules in plant and animal cells. In addition, the book covers the special cases of nuclear import of Agrobacterium T-DNA during plant genetic transformation, nuclear import and export of animal viruses, and nuclear intake of foreign DNA. A chapter on research methods to study nuclear transport concludes the book.

Agrobacterium Protocols

Author : Kan Wang
Publisher : Springer Science & Business Media
ISBN 13 : 1597451304
Total Pages : 474 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Agrobacterium Protocols by : Kan Wang

Download or read book Agrobacterium Protocols written by Kan Wang and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Genetic Transformation of Plants

Author : John Flex Jackson
Publisher : Springer Science & Business Media
ISBN 13 : 3662074249
Total Pages : 202 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Genetic Transformation of Plants by : John Flex Jackson

Download or read book Genetic Transformation of Plants written by John Flex Jackson and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.

Transgenic Crops II

Author : Y.P.S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642569013
Total Pages : 362 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Transgenic Crops II by : Y.P.S. Bajaj

Download or read book Transgenic Crops II written by Y.P.S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: There has been tremendous progress in the genetic transformation of agricultural crops, and plants resistant to insects, herbicides, and diseases have been produced, field tested, and patented. This book compiles this information on various fruits and vegetables.

Transgenic Plants

Author : Adina Breiman
Publisher : World Scientific
ISBN 13 : 1783262540
Total Pages : 392 pages
Book Rating : 4.7/5 (832 download)

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Book Synopsis Transgenic Plants by : Adina Breiman

Download or read book Transgenic Plants written by Adina Breiman and published by World Scientific. This book was released on 1997-10-13 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comes with an Appendix on Intellectual Properties and Commercialisation of Transgenic Plants by John Barton (Stanford University Law School)This timely and important book presents the essence of transgenic plant production. This activity is being pursued by many investigators and interesting results are rapidly accumulating. The basic methodologies have been developed and the transformation of additional plant species is more an “engineering”/biotechnology problem than a matter of developing new scientific concepts. This book reviews the available methodologies and devotes chapters to transgenic plants that were produced for crop improvement and for yielding valuable products. Also, information is provided on the ability to regulate the expression of alien genes in specific organs and in response to defined effectors and environmental conditions. Finally, transgenic plants may have commercial value, therefore the issues of intellectual property and other aspects of commercialisation are handled in a special appendix.In addition to providing a comprehensive overview of transgenic plant production for investigators engaged in a specific niche of this endeavour, this book will be of interest to all students of plant biology and to those who consider producing transgenic plants in the future. Plant breeders and commercial companies engaged in seed production will definitely benefit from this book.

Transgenic Crops I

Author : Y.P.S. Bajaj
Publisher : Springer Science & Business Media
ISBN 13 : 3642596126
Total Pages : 409 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Transgenic Crops I by : Y.P.S. Bajaj

Download or read book Transgenic Crops I written by Y.P.S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently. there has been tremendous progress in the genetic transformation of agricultural crops, and plants resistant to insects, herbicides, and diseases have been produced, field tested and patented. Transgenic Crops I compiles this information on cereals, grasses, legumes, and oilseed crops. It comprises 25 chapters and is divided into two sections: I. Cereals and Grasses: wheat, rice, maize, barley, sorghum, pearl millet, triticale, Agrostis spp., Cenchrus ciliaris, Dactylis glomerata, Festuca arundinacea, Lolium spp., and sugarcane. II. Legumes and Oilseed Crops: Arachis hypogaea, Brassica juncea, Brassica napus, Cicer arietinum, Glycine max, Gossypium hirsutum, Helianthus annuus, Lens culinaris, Linum usitatissimum, Sinapis alba, Trifolium, and Vicis narbonensis. This book is of special interest to advanced students, teachers, and research workers in the field of plant breeding, genetics, molecular biology, plant tissue culture, and plant biotechnology in general.