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Author : Patrick E. Guire
Publisher :
ISBN 13 :
Total Pages : 88 pages
Book Rating : 4.:/5 (22 download)
Download or read book Affinity Cross-linking Agents for Enzyme Stabilization and Immobilization written by Patrick E. Guire and published by . This book was released on 19?? with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 :
Total Pages : 494 pages
Book Rating : 4.F/5 ( download)
Download or read book Immobilized Affinity Ligand Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 1992-08-27 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References
Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 : 0080568726
Total Pages : 1233 pages
Book Rating : 4.0/5 (85 download)
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Author : Oak Ridge National Laboratory
Publisher :
ISBN 13 :
Total Pages : 944 pages
Book Rating : 4.:/5 (319 download)
Download or read book Inventory of energy research and development--1973-1975 written by Oak Ridge National Laboratory and published by . This book was released on 1976 with total page 944 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : United States. Congress. Senate. Select Committee on Small Business
Publisher :
ISBN 13 :
Total Pages : 1198 pages
Book Rating : 4.0/5 (18 download)
Download or read book Energy Research and Development and Small Business written by United States. Congress. Senate. Select Committee on Small Business and published by . This book was released on 1975 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Oak Ridge National Laboratory
Publisher :
ISBN 13 :
Total Pages : 952 pages
Book Rating : 4.:/5 (36 download)
Download or read book Inventory of Energy Research and Development, 1973-1975: Introduction, Energy research and development projects written by Oak Ridge National Laboratory and published by . This book was released on 1976 with total page 952 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jose M. Guisan
Publisher : Humana
ISBN 13 : 9781493959884
Total Pages : 0 pages
Book Rating : 4.9/5 (598 download)
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2016-08-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Author : National Science Foundation (U.S.). Directorate for Engineering and Applied Science
Publisher :
ISBN 13 :
Total Pages : 256 pages
Book Rating : 4.3/5 (91 download)
Download or read book Recent Research Reports written by National Science Foundation (U.S.). Directorate for Engineering and Applied Science and published by . This book was released on 1979 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : United States. Congress. Senate. Select Committee on Small Business
Publisher :
ISBN 13 :
Total Pages : 1178 pages
Book Rating : 4.:/5 (327 download)
Download or read book Energy Research and Development and Small Business: how much? How much more from small business? How soon? written by United States. Congress. Senate. Select Committee on Small Business and published by . This book was released on 1975 with total page 1178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Academic Press
ISBN 13 : 0128050799
Total Pages : 232 pages
Book Rating : 4.1/5 (28 download)
Download or read book Marine Enzymes Biotechnology: Production and Industrial Applications, Part II - Marine Organisms Producing Enzymes written by and published by Academic Press. This book was released on 2016-10-18 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Marine Enzymes Biotechnology: Production and Industrial Applications, Part II - Marine Organisms Producing Enzymes provides a huge treasure trove of information on marine organisms. Nowadays, marine organisms are good candidates for enzymes production and have been recognized as a rich source of biological molecules that are of potential interest to various industries. Marine enzymes such as amylases, carboxymethylcellulases, proteases, chitinases, keratinases, xylanases, agarases, lipases, peroxidase and tyrosinases are widely used in the industry for the manufacture of pharmaceuticals, foods, beverages, and confectioneries, as well as in textile and leather processing, and in waste water treatment. The majority of the enzymes used in the industry are of microbial origin because microbial enzymes are relatively more stable than the corresponding enzymes derived from plants and animals. Focuses on the isolation, characterization, and industrial application of marine enzymes Provides current trends and development of industrial important marine enzymes, including amylases, carboxymethylcellulases, proteases, chitinases, keratinases, xylanases, agarases, lipases, peroxidase, and tyrosinases Presents insights into current trends and approaches for marine enzymes
Author : Linqiu Cao
Publisher : John Wiley & Sons
ISBN 13 : 3527607080
Total Pages : 578 pages
Book Rating : 4.5/5 (276 download)
Download or read book Carrier-bound Immobilized Enzymes written by Linqiu Cao and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes. A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.
Author : Maurizio Benaglia
Publisher : John Wiley & Sons
ISBN 13 : 3527345094
Total Pages : 492 pages
Book Rating : 4.5/5 (273 download)
Download or read book Catalyst Immobilization written by Maurizio Benaglia and published by John Wiley & Sons. This book was released on 2020-04-06 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive resource on techniques and applications for immobilizing catalysts Catalyst Immobilization: Methods and Applications covers catalyst immobilization topics including technologies, materials, characterization, chemical activity, and recyclability. The book also presents innovative applications for supported catalysts, such as flow chemistry and machine-assisted organic synthesis. Written by an international panel of expert contributors, this book outlines the general principles of catalyst immobilization and explores different types of supports employed in catalyst heterogenization. The book?s chapters examine the immobilization of chiral organocatalysts, reactions in flow reactors, 3D printed devices for catalytic systems, and more. Catalyst Immobilization offers a modern vision and a broad and critical view of this exciting field. This important book: -Offers a guide to supported and therefore recyclable catalysts, which is one of the most important tools for developing a highly sustainable chemistry -Presents various immobilization techniques and applications -Explores new trends, such as 3D printed devices for catalytic systems -Contains information from a leading international team of authors Written for catalytic chemists, organic chemists, process engineers, biochemists, surface chemists, materials scientists, analytical chemists, Catalyst Immobilization: Methods and Applications presents the latest developments and includes a review of the innovative trends such as flow chemistry, reactions in microreactors, and beyond.
Author : Maria Lujan Ferreira
Publisher : Academic Press
ISBN 13 : 0323913776
Total Pages : 460 pages
Book Rating : 4.3/5 (239 download)
Download or read book Biocatalyst Immobilization written by Maria Lujan Ferreira and published by Academic Press. This book was released on 2022-11-12 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends. Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization Features chapter contributions from international leaders in the field
Author : Alka Dwevedi
Publisher : Academic Press
ISBN 13 : 0128192070
Total Pages : 189 pages
Book Rating : 4.1/5 (281 download)
Download or read book Polymeric Supports for Enzyme Immobilization written by Alka Dwevedi and published by Academic Press. This book was released on 2021-06-22 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme immobilization has been approached for finding solutions for various critical problems associated with industries, medicine, environment, agriculture, etc. Especially since last decades, several innovative researches have come up to look for enhancing catalytic efficiency, reusability of immobilized enzyme and longer stability by introducing range of immobilizing supports, supports modifiers through introduction of several chemical agents (non-toxic) and adopting innovative enzyme immobilization methods. In the present book, polymeric supports have been focussed for enzyme immobilization, especially due to their versatility in immobilizing different enzymes for different large scale enzyme reactors to be used for several applications. Especially, polymers can be modified according to applications and enzyme properties which have made it supports of choice for all several enzyme based applications. Polymeric Supports for Enzyme Immobilization: Opportunities and Applications offers in-depth discussions of known polymeric enzyme support materials, reaction processes, and optimized methods to enhance enzyme immobilization. Case-based chapters examine methods of enzyme immobilization onto various polymeric supports, their surface chemistry and physical morphology followed by implementation of polymers based immobilized enzymes in various applications, viz. medicine, environment, industries, clean energy, disease diagnosis, drug delivery etc. This book has prime focus to allow several researchers across the world to provide updated technological details and incite to contribute more innovative work in coming years to find solutions to several critical problems. Offers an in-depth, case-driven discussion of known polymeric enzyme support materials, associated reaction processes, and methods to enhance enzyme immobilization Provides optimal strategies for various enzymes, processes, and applications, considering the enzyme itself, substrate, and available support properties Provides complete details on applications of polymeric based immobilized enzymes in various applications ranging from chemical; or pharmaceutical synthesis, food processing, bioremediation, industrial catalysis, etc.
Author :
Publisher :
ISBN 13 :
Total Pages : 684 pages
Book Rating : 4.:/5 (31 download)
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1979 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lists citations with abstracts for aerospace related reports obtained from world wide sources and announces documents that have recently been entered into the NASA Scientific and Technical Information Database.
Author :
Publisher :
ISBN 13 :
Total Pages : 748 pages
Book Rating : 4.3/5 (129 download)
Download or read book Energy Research Abstracts written by and published by . This book was released on 1979 with total page 748 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jose M. Guisan
Publisher : Humana
ISBN 13 : 9781071602171
Total Pages : 495 pages
Book Rating : 4.6/5 (21 download)
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2021-01-29 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
