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A Practical Guide To Scanning Electron Microscopy In The Biosciences
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Book Synopsis A Practical Guide to Scanning Electron Microscopy in the Biosciences by : Gerhard Wanner
Download or read book A Practical Guide to Scanning Electron Microscopy in the Biosciences written by Gerhard Wanner and published by John Wiley & Sons. This book was released on 2022-09-21 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise and authoritative introduction to scanning electron microscopy in the biological sciences In A Practical Guide to Scanning Electron Microscopy distinguished electron microscopist Gerhard Wanner delivers a practical handbook for biological scientists working with microbial, plant, and animal cells and tissues, enabling them to successfully apply scanning electron microscopy (SEM) to their object of study. The book begins with an introduction to the principles of electron microscopy and the operation of electron microscopes before moving on to describe the preparation and mounting of specimens. It also explores the process of recoding images and their subsequent analysis, along with a wide range of advanced microscopy techniques, including cryo-SEM, FIB-SEM tomography, and stereo-SEM. Scanning Electron Microscopy in the Biosciences contains hundreds of carefully selected microscopic images, as well as hands-on, step-by-step guidance required to perform a successful TEM experiment. Readers will also find: Thorough introductions to optics, electron microscopy, electrons, and the components of electron microscopes In-depth examinations of the preparation of biological specimens and specimen mounting for scanning electron microscopy A comparison of different SEM modes and their strengths and weaknesses An introduction to novel techniques such as correlative light and electron microscopy (CLEM), array tomography, and cryo-scanning electron microscopy Perfect for cell biologists and microbiologists, A Practical Guide to Scanning Electron Microscopy in the Biosciences also belongs in the libraries of neurobiologists and biophysicists.
Book Synopsis A Practical Guide to Scanning Electron Microscopy in the Biosciences by : Gerhard Wanner
Download or read book A Practical Guide to Scanning Electron Microscopy in the Biosciences written by Gerhard Wanner and published by John Wiley & Sons. This book was released on 2022-09-08 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise and authoritative introduction to scanning electron microscopy in the biological sciences In A Practical Guide to Scanning Electron Microscopy distinguished electron microscopist Gerhard Wanner delivers a practical handbook for biological scientists working with microbial, plant, and animal cells and tissues, enabling them to successfully apply scanning electron microscopy (SEM) to their object of study. The book begins with an introduction to the principles of electron microscopy and the operation of electron microscopes before moving on to describe the preparation and mounting of specimens. It also explores the process of recoding images and their subsequent analysis, along with a wide range of advanced microscopy techniques, including cryo-SEM, FIB-SEM tomography, and stereo-SEM. Scanning Electron Microscopy in the Biosciences contains hundreds of carefully selected microscopic images, as well as hands-on, step-by-step guidance required to perform a successful TEM experiment. Readers will also find: Thorough introductions to optics, electron microscopy, electrons, and the components of electron microscopes In-depth examinations of the preparation of biological specimens and specimen mounting for scanning electron microscopy A comparison of different SEM modes and their strengths and weaknesses An introduction to novel techniques such as correlative light and electron microscopy (CLEM), array tomography, and cryo-scanning electron microscopy Perfect for cell biologists and microbiologists, A Practical Guide to Scanning Electron Microscopy in the Biosciences also belongs in the libraries of neurobiologists and biophysicists.
Book Synopsis Diagnostic Electron Microscopy by : John Stirling
Download or read book Diagnostic Electron Microscopy written by John Stirling and published by John Wiley & Sons. This book was released on 2013-01-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.
Book Synopsis A Practical Guide to Transmission Electron Microscopy: Advanced microscopy by : Zhiping Luo
Download or read book A Practical Guide to Transmission Electron Microscopy: Advanced microscopy written by Zhiping Luo and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Practical Guide to Transmission Electron Microscopy, Volume II by : Zhiping Luo
Download or read book A Practical Guide to Transmission Electron Microscopy, Volume II written by Zhiping Luo and published by Momentum Press. This book was released on 2015-12-23 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transmission Electron Microscope (TEM) is a very powerful tool for characterizing various types of materials. Using a light microscope, the imaging resolution is at several hundred nanometers, and for a Scanning Electron Microscope (SEM) at several nanometers. The imaging resolution of the TEM, however, can routinely reach several angstroms on a modem instrument. In addition, the TEM can also provide material structural information, since the electrons penetrate through the thin specimens, and chemical compositional information due to the strong electron-specimen atom interactions. This book provides a concise practical guide to the TEM user, starting from the beginner level, including upper-division undergraduates, graduates, researchers, and engineers, on how to learn TEM efficiently in a short period of time. Volume I covers the instrumentation, sample preparation, fundamental diffraction, imaging, analytical microscopy, and some newly developed microscopy techniques. This book may serve as a textbook for a TEM course or workshop, or a reference book for the TEM user to improve their TEM skills.
Book Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck
Download or read book Biological Field Emission Scanning Electron Microscopy, 2 Volume Set written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Book Synopsis Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis by : Patrick Echlin
Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Book Synopsis Scanning Electron Microscopy for the Life Sciences by : Heide Schatten
Download or read book Scanning Electron Microscopy for the Life Sciences written by Heide Schatten and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM.
Book Synopsis Practical Electron Microscopy by : Elaine Evelyn Hunter
Download or read book Practical Electron Microscopy written by Elaine Evelyn Hunter and published by Cambridge University Press. This book was released on 1993-09-24 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added--on immuno electron microscopy using colloidal gold and on useful specialized techniques.
Book Synopsis A Practical Guide to Scanning Probe Microscopy by : Rebecca Howland
Download or read book A Practical Guide to Scanning Probe Microscopy written by Rebecca Howland and published by . This book was released on 1998-06 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides basic information about scanning probe microscopes (SPMs), which are used in a wide variety of disciplines, including fundamental surface science, routine surface roughness analysis, & spectacular three-dimensional imaging -- from atoms of silicon to micron-sized protrusions on the surface of a living cell. Issues covered in this handbook range from fundamental physics of SPMs to their practical capabilities & instrumentation. Examples of applications are included throughout the text, & several application-specific articles are listed at the end of each chapter.
Book Synopsis Scanning Electron Microscopy for the Life Sciences by : Heide Schatten
Download or read book Scanning Electron Microscopy for the Life Sciences written by Heide Schatten and published by Cambridge University Press. This book was released on 2012-12-06 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM.
Book Synopsis Biological Electron Microscopy by : Michael J. Dykstra
Download or read book Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
Book Synopsis Introduction to Scanning Transmission Electron Microscopy by : Dr Robert Keyse
Download or read book Introduction to Scanning Transmission Electron Microscopy written by Dr Robert Keyse and published by Routledge. This book was released on 2018-12-19 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1997 was the 'Year of the Electron' because it marked the centenary pf the celebrated discovery of the smallest of the fundamental particles that make up ordinary matter, and which has proved to have so many remarkable properties that, after light, it has become the most widley used of the particles in scientific and technogical applications. STEM is a discipline of importance to a growing number of microscopists. This book is essential reading for undergraduates, postgraduates and researchers requiring an up-to-date and comprehensive introduction to this rapidly growing, state of the art technique.
Book Synopsis Biological Low-Voltage Scanning Electron Microscopy by : James Pawley
Download or read book Biological Low-Voltage Scanning Electron Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2007-12-03 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.
Book Synopsis Practical Electron Microscopy by : Elaine E. Hunter
Download or read book Practical Electron Microscopy written by Elaine E. Hunter and published by Praeger Publishers. This book was released on 1984-01-01 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Introduction to Biological Scanning Electron Microscopy by : M. A. Hayat
Download or read book Introduction to Biological Scanning Electron Microscopy written by M. A. Hayat and published by . This book was released on 1978 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Scanning and Transmission Electron Microscopy by : Stanley L. Flegler
Download or read book Scanning and Transmission Electron Microscopy written by Stanley L. Flegler and published by Oxford University Press, USA. This book was released on 1995 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: A core textbook for courses on electron microscopy Ideal for use in any laboratory, this book presents the practical and theoretical fundamentals of scanning and transmission electron microscopy. Clear and concise explanations coupled with instructive diagrams and photographs guide you through: * microscope operation * image production * analytical techniques Specimen preparation is discussed in detail with emphasis on specific parameters for biological specimens. This unique book covers the essentials of scanning and transmission electron microscopy while leaving the laboratory particulars to individual discretion. Unmatched in scope and clarity, this text offers the best introduction to scanning and transmission electron microscopy available.
