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A Flexible System Of Enzymatic Analysis
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Book Synopsis A Flexible System of Enzymatic Analysis by : Oliver Lowry
Download or read book A Flexible System of Enzymatic Analysis written by Oliver Lowry and published by Elsevier. This book was released on 2012-12-02 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Flexible System of Enzymatic Analysis is a multipurpose manual of laboratory methods that offers a systematic scheme for the analysis of biological materials from the level of the wholeorgan down to the single cell and beyond. It intends to guide the development of methods, the refinement of old ones, and the adaptation in general of methods to almost any scale of sensitivity. This manual is organized into three parts: a general section, one on quantitative histochemistry, and an appendix containing information that may be useful to have at the bench. The general section is comprised of nine chapters that focus on properties of the pyridine nucleotides, kinetics, and glassware, as well as on improvement, modification, adaptation, trouble shooting, and development of methods. This part also describes the preparation of tissues for analysis, the enzymatic cycling methods, and a compendium of 36 metabolite assays. The quantitative histochemistry section is comprised of four chapters that include information on the preparation of frozen-dried material and dissection of samples for analysis; the fishpole balance for weighing samples; and the generalities of analysis with emphasis on the ""oil well technique."" This book will be useful to novices as well as experts who are familiar with other analytical styles.
Book Synopsis Enzymatic Analysis by : Janet V. Passonneau
Download or read book Enzymatic Analysis written by Janet V. Passonneau and published by Springer Science & Business Media. This book was released on 2008-02-07 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
Book Synopsis Transmethylations and the Central Nervous System by : V. M. Andreoli
Download or read book Transmethylations and the Central Nervous System written by V. M. Andreoli and published by Springer Science & Business Media. This book was released on 2013-12-11 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: V M. ANDREOLl, A. AGNOLI, and C. FAZIO The transfer of a methyl group from a donor to an acceptor compound is a fundamental biochemie al process long since known to biologists; the process is involved, for instance, in the metabolism of porphyrins, nuc1eic acids, and fatty acids. Only recently, however, did transmethylation processes reveal their decisive role in CNS biochemistry - namely with the discovery that such processes are linked with the biogenie amines that have been conc1usively identified as the chemical mediators of neu ronal transmission and, more broadly, of behavior. The first suggestion that transmethylation processes might be involved in the origina tion of certain mental diseases came from Harley-Mason (1952), who noticed that many of the hallucinogenic substances known at that time contained methyl radicals, and par ticularly that mescaline represented the product of O-methylation of dopamine in posi tions 3, 4, and 5. This hypothesis was put forward when the O-methylation of catechol amines by catechol-O-methyltransferases had not yet been described. Harley-Mason further proposed that in the living organism the process of O-methyla tion might follow a "deviant" metabolie pathway, and that the accumulation of abnor mal methylated metabolites endowed with hallucinogenic properties might be responsi ble for the implementation of some mental diseases, notably schizophrenia. He also called attention to a substance, 3,4-dimethoxyphenylethylamine (3,4-DMPEA) as a possible psychotogenic moleeule, on the strength of its capacity for producing cata tonia in experimental animals
Book Synopsis Handbook of Phycological Methods: Culture methods and growth measurements, edited by J. R. Stein by : Janet R. Stein-Taylor
Download or read book Handbook of Phycological Methods: Culture methods and growth measurements, edited by J. R. Stein written by Janet R. Stein-Taylor and published by Cambridge University Press. This book was released on 1973 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolation and purification; General equipment and methods; Special culture methods; Growth measurements; Bioassay.
Book Synopsis Handbook of Muscle Foods Analysis by : Leo M.L. Nollet
Download or read book Handbook of Muscle Foods Analysis written by Leo M.L. Nollet and published by CRC Press. This book was released on 2008-11-10 with total page 990 pages. Available in PDF, EPUB and Kindle. Book excerpt: In today's nutrition-conscious society, there is a growing awareness among meat scientists and consumers about the importance of the essential amino acids, vitamins, and minerals found in muscle foods. Handbook of Muscle Foods Analysis provides a comprehensive overview and description of the analytical techniques and application methodologies for t
Book Synopsis Enzymatic Analysis by : Janet V. Passonneau
Download or read book Enzymatic Analysis written by Janet V. Passonneau and published by . This book was released on 2014-09-01 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Enzyme Immunoassay by : Edward T. Maggio
Download or read book Enzyme Immunoassay written by Edward T. Maggio and published by CRC Press. This book was released on 2018-01-18 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a projection of the future, but mostly it reveals an overview of the field as it exists as the present time. It aims to serve to spawn further growth in ideas and encourage applications to increasingly broader segments of both clinical and general analytical chemistry fields.
Download or read book Enzyme Assays written by Robert Eisenthal and published by Practical Approach. This book was released on 2002 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Book Synopsis Frontiers in Biosensorics II by : F.W. Scheller
Download or read book Frontiers in Biosensorics II written by F.W. Scheller and published by Birkhäuser. This book was released on 2013-03-12 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume II focuses on the state-of-the-art technologies and applications of various types of sensors. It presents different analyzers and their operational parameters in many areas of practical use. Moreover, aspects of the marketability of biosensors are covered in the context of established conventional analytical techniques as well as under consideration of the needs, habits and beliefs of consumers. A picture thus emerges of how applications can be expanded in the future and novel markets can be opened up.
Book Synopsis Trends in Enzyme Histochemistry and Cytochemistry by : David Evered
Download or read book Trends in Enzyme Histochemistry and Cytochemistry written by David Evered and published by John Wiley & Sons. This book was released on 2009-09-16 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Book Synopsis Methods in Mammalian Reproduction by : Joseph C. Jr. Daniel
Download or read book Methods in Mammalian Reproduction written by Joseph C. Jr. Daniel and published by Elsevier. This book was released on 2012-12-02 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Mammalian Reproduction presents some of the techniques for manipulating, analyzing, observing, testing, and generally experimenting with mammalian mothers and their gametes and embryos. Mammalian reproduction involves an intimate relationship between mother and embryo. The first 18 chapters are arranged in an order that follows a developmental sequence from oocyte to fetal organs and the remaining seven chapters deal with the maternal side of the relationship. With strong focus on laboratory rodents and lagomorphs, the book starts with an introduction to in vitro oocyte maturation and experimental production of mammalian parthenogenetic. It goes on to describe the microtechniques in pre-implantation of embryos, production of chimeras, techniques for early embryonic tissue separation, mammalian embryo preservation by freezing, and in vitro development of whole mouse embryos beyond the implantation stage. Chapters 11-15 discuss the in vitro implantation of mouse blastocysts, advances in rabbit embryo and in large mammal embryo cultures, embryo transfer in large domestic mammals, and manipulation of marsupial embryos and pouch young. The following chapters cover reproduction experiments using marsupials, domestic farm species, and primates including humans. Finally, the concluding chapters tackle the use of amniocentesis in prenatal diagnosis, collection and analysis of female genital tract secretions, analysis of antifertility action of intrauterine devices, and surgical induction of endometriosis. This book will be helpful to students, teachers, researchers, and clinical researchers who demand for more and better procedures for analysis of mammalian reproduction.
Book Synopsis Digestive System by : Thomas C. Jones
Download or read book Digestive System written by Thomas C. Jones and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: The International Life Sciences Institute (ILSI) was creat ed to promote cooperative efforts toward solving critical health and safety questions involving foods, drugs, cosmet ics, chemicals, and other aspects of the environment. The Officers and Trustees believe that questions regarding health and safety are best resolved when government and industry rely on scientific investigations, analyses, and reviews by independent experts. Further, the scientific aspects of an issue should be examined and discussed on an international basis, separate from the political concerns of individual companies. ILSI is pleased to sponsor this set of monographs on the pathology of laboratory animals. This project will be use ful in improving the scientific basis for the application of pathologic techniques to health and safety evaluation of substances in our environment. The world wide distribu tion of the authors, editors, and Editorial Board who are creating these monographs strengthens the expectation that international communication and cooperation will al so be strengthened.
Download or read book Urinary Enzymes written by Klaus Jung and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interest and research in urinary enzymology were incited about three decades ago by reports that urinary enzymes are elevated in diseases of the kidney and urinary tract. Of the more than 40 hydrolases, oxidoreductases, transferases, and lyases identified in human and animal urine, only ten or so are being used as diagnostic indicators. Recognition of the quantitative distribution of enzymes in the various anatomical and functional parts of the nephron and advances in our understanding of the handling of proteins by the kidney have made it possible to associate urinary enzyme activity patterns with physiological and pathophysio logical functions of the nephron. Confidence in the diagnostic value of urinary enzymes is not unanimous among clinicians and among scientists. The main reason for the difference in opinion may well be that the variability in data exceeds the variability one is accustomed to in the diagnostic enzymology of blood plasma enzymes. In contrast to plasma enzymes, which are protected by an "enzyme friendly" milieu, enzymes released into the urine encounter an "enzyme hostile" environ ment: no or little protective protein, variable pH, variable volume, variable metabolite and salt concentrations, variable concentrations of enzyme in hibitors. Through advances in methodology some of these factors can now be controlled; standardization of urine collection periods and preanalytical treat ment are as important as optimization of assay methods.
Book Synopsis Microchemical Analysis of Nervous Tissue by : Neville N. Osborne
Download or read book Microchemical Analysis of Nervous Tissue written by Neville N. Osborne and published by Elsevier. This book was released on 2013-10-22 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microchemical Analysis of Nervous Tissue focuses on the use of microbiochemical methods in the analysis of nervous tissue, with emphasis on those related to the study of amines, amino acids, phospholipids, and proteins. Special attention is paid to the choice of biological material and the various procedures used for the isolation by dissection of defined components of the nervous system. Comprised of 10 chapters, this volume begins with an overview of microprocedures used in neurochemistry, followed by a discussion on the importance of choosing the biological material for microanalysis. The isolation of nervous tissue for analysis is then considered, with particular reference to invertebrate neurons; cell components from fresh, "fixed," freeze-dried, and frozen impregnated tissue; and discrete areas of nervous tissue. Subsequent chapters describe some instruments and glassware used in microprocedures, along with the applications of such procedures; general techniques used in microprocedures; microdetermination of phospholipids as well as amines and amino acids as dansyl derivatives; and microelectrophoresis of proteins. This book will be of interest to molecular biologists, microbiologists, physiologists, and neurochemists.
Book Synopsis Histochemical and Immunohistochemical Techniques by : Peter H. Bach
Download or read book Histochemical and Immunohistochemical Techniques written by Peter H. Bach and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.
Author :Hans-Ferdinand Linskens Publisher :Springer Science & Business Media ISBN 13 :3642836119 Total Pages :301 pages Book Rating :4.6/5 (428 download)
Book Synopsis Physical Methods in Plant Sciences by : Hans-Ferdinand Linskens
Download or read book Physical Methods in Plant Sciences written by Hans-Ferdinand Linskens and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: Latest techniques for the analysis of plant cell or tissue structure and the registration of physiological pathways are topics of this volume. The subjects include: - Laser Doppler Vibrometer Measurements of Leaves; - Laster Physical Methods. Laser Microprobe Mass Spectrometry; - Triplet States in Photosynthesis: Linear Dichroic Optical Difference Spectra via Magnetic Resonance; - Fast Atom Bombardment Mass Spectrometry; -Microdissection and Biochemical Analysis of Plant Tissues; - Photoacoustic Spectroscopy - Photoacoustic and Photothermal Effects; - Membrane Operational Impedance of Spectra of Plant Cell.
Book Synopsis Research Procedures and Data Analysis by : James Wilson
Download or read book Research Procedures and Data Analysis written by James Wilson and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt:
