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X Ray Microanalysis In Biology
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Book Synopsis Scanning Electron Microscopy and X-Ray Microanalysis by : Joseph Goldstein
Download or read book Scanning Electron Microscopy and X-Ray Microanalysis written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Book Synopsis X-ray Microanalysis for Biologists by : Alice Warley
Download or read book X-ray Microanalysis for Biologists written by Alice Warley and published by Ashgate Publishing. This book was released on 1997 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The smart way to learn how to build InfoPath forms for SharePoint - one step at a time. Design and build forms without writing code, add approval workflows to your forms, integrate data, create and use forms in the cloud.
Book Synopsis X-ray Microanalysis in Biology by : David C. Sigee
Download or read book X-ray Microanalysis in Biology written by David C. Sigee and published by Cambridge University Press. This book was released on 1993-04-22 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes an integrated approach to the use of X-ray microanalysis in biology.
Book Synopsis Practical Scanning Electron Microscopy by : Joseph Goldstein
Download or read book Practical Scanning Electron Microscopy written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 598 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the spring of 1963, a well-known research institute made a market survey to assess how many scanning electron microscopes might be sold in the United States. They predicted that three to five might be sold in the first year a commercial SEM was available, and that ten instruments would saturate the marketplace. In 1964, the Cambridge Instruments Stereoscan was introduced into the United States and, in the following decade, over 1200 scanning electron microscopes were sold in the U. S. alone, representing an investment conservatively estimated at $50,000- $100,000 each. Why were the market surveyers wrongil Perhaps because they asked the wrong persons, such as electron microscopists who were using the highly developed transmission electron microscopes of the day, with resolutions from 5-10 A. These scientists could see little application for a microscope that was useful for looking at surfaces with a resolution of only (then) about 200 A. Since that time, many scientists have learned to appreciate that information content in an image may be of more importance than resolution per se. The SEM, with its large depth of field and easily that often require little or no sample prepara interpreted images of samples tion for viewing, is capable of providing significant information about rough samples at magnifications ranging from 50 X to 100,000 X. This range overlaps considerably with the light microscope at the low end, and with the electron microscope at the high end.
Book Synopsis Scanning Electron Microscopy and X-ray Microanalysis by : Graham Lawes
Download or read book Scanning Electron Microscopy and X-ray Microanalysis written by Graham Lawes and published by . This book was released on 1987 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Electron Probe Microanalysis by : A. J. Tousimis
Download or read book Electron Probe Microanalysis written by A. J. Tousimis and published by . This book was released on 1969 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biomedical Applications of Microprobe Analysis by : Peter Ingram
Download or read book Biomedical Applications of Microprobe Analysis written by Peter Ingram and published by Academic Press. This book was released on 1999-10-29 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter
Book Synopsis Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis by : Patrick Echlin
Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Book Synopsis X-Ray Optics and X-Ray Microanalysis by : H. H. Pattee
Download or read book X-Ray Optics and X-Ray Microanalysis written by H. H. Pattee and published by Elsevier. This book was released on 2013-10-22 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray Optics and X-ray Microanalysis covers the proceedings of the Symposium on X-ray Optics and X-ray Microanalysis, held at Stanford University on August 22-24, 1962. The book focuses on X-ray microscopy, microradiography, radiation and irradiation, and X-ray microanalysis. The selection first offers information on the methods of X-ray microscopy and X-ray absorption microanalysis. Discussions focus on X-ray scanning microscopy, contact microradiography, point projection microscopy, and total dry-weight determinations. The text then takes a look at X-ray microanalysis in biology and medicine; electron microscopic enlargements of X-ray absorption micrographs; and automation in microradiography. The publication examines the production of Fresnel zone plates for extreme ultraviolet and soft X radiation; quantitative microradiographic studies of human epidermis; and irradiation effect on total organic nerve-cell material determined by integrating X-ray absorption. The manuscript then reviews the calculation of fluorescence excited by characteristic radiation in the X-ray microanalyzer and the method for calculating the absorption correction in electron-probe microanalysis. The selection is a valuable reference for readers interested in X-ray technology.
Author :Rudolf A. Steinbrecht Publisher :Springer Science & Business Media ISBN 13 :3642728154 Total Pages :308 pages Book Rating :4.6/5 (427 download)
Book Synopsis Cryotechniques in Biological Electron Microscopy by : Rudolf A. Steinbrecht
Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Book Synopsis X-ray Microanalysis in Biology by : M. A. Hayat
Download or read book X-ray Microanalysis in Biology written by M. A. Hayat and published by . This book was released on 1980 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis X-ray Microanalysis in Biology and Medicine by : Arne Engström
Download or read book X-ray Microanalysis in Biology and Medicine written by Arne Engström and published by . This book was released on 1962 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Plant Cell Biology by : David W. Galbraith
Download or read book Methods in Plant Cell Biology written by David W. Galbraith and published by Academic Press. This book was released on 1995 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.
Book Synopsis MICRON HIGH RESOLUTION MICROSCOPY AND X-RAY MICROANALYSIS IN BIOLOGY by :
Download or read book MICRON HIGH RESOLUTION MICROSCOPY AND X-RAY MICROANALYSIS IN BIOLOGY written by and published by . This book was released on 1972 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :M. A. Nasser Hajibagheri Publisher :Springer Science & Business Media ISBN 13 :1592592015 Total Pages :292 pages Book Rating :4.5/5 (925 download)
Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri
Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Book Synopsis Progress in Medical Radiation Physics by : Colin G. Orton
Download or read book Progress in Medical Radiation Physics written by Colin G. Orton and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Progress in Medical Radiation Physics series presents in-depth reviews of many of the significant developments resulting from the application of physics to medicine. This series is intended to span the gap between research papers published in scientific journals, which tend to lack details, and complete textbooks or theses, which are usually far more detailed than necessary to provide a working knowledge of the subject. Each chapter in this series is designed to provide just enough information to enable readers to both fully understand the development described and apply the technique or concept, if they so desire. Thorough references are provided for those who wish to consider the original literature. In this way, it is hoped that the Progress in Medical Radiation Physics series will be a catalyst encouraging medical physicists to apply new techniques and developments in their daily practice. Colin G. Orton ix Contents 1-1. The Tracking Cobalt Project: From Moving-Beam Therapy to Three-Dimensional Programmed Irradiation W. A. Jennings 1. Introduction 2. Establishing Moving-Beam Techniques at the Royal Northern Hospital, 1945-1955 4 2.1. Alternative Moving-Beam Techniques 4 2.2.
Book Synopsis Introduction to Electron Microscopy for Biologists by :
Download or read book Introduction to Electron Microscopy for Biologists written by and published by Academic Press. This book was released on 2008-10-22 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
