Western Blotting

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Western Blotting Guru

Author : Ayaz Najafov
Publisher : Academic Press
ISBN 13 : 0128135387
Total Pages : 100 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Western Blotting Guru by : Ayaz Najafov

Download or read book Western Blotting Guru written by Ayaz Najafov and published by Academic Press. This book was released on 2017-07-07 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types. Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments Contains unique good practices and tips that are indispensable for the beginner and expert alike Features special cases with applications of immunoblotting optimization Includes detailed appendices with tables, figures and key protocols Provides troubleshooting tips for various types of modifications of standard protocols Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem

Western Blotting

Author : Biji T. Kurien
Publisher : Humana
ISBN 13 : 9781493926930
Total Pages : 0 pages
Book Rating : 4.9/5 (269 download)

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Book Synopsis Western Blotting by : Biji T. Kurien

Download or read book Western Blotting written by Biji T. Kurien and published by Humana. This book was released on 2015-06-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

Western Blotting for the Non-Expert

Author : Biji T. Kurien
Publisher : Springer Nature
ISBN 13 : 3030706842
Total Pages : 467 pages
Book Rating : 4.0/5 (37 download)

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Book Synopsis Western Blotting for the Non-Expert by : Biji T. Kurien

Download or read book Western Blotting for the Non-Expert written by Biji T. Kurien and published by Springer Nature. This book was released on 2021-07-24 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..

Protocols in Advanced Genomics and Allied Techniques

Author : Aruna Pal
Publisher : Springer Nature
ISBN 13 : 1071618180
Total Pages : 762 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Protocols in Advanced Genomics and Allied Techniques by : Aruna Pal

Download or read book Protocols in Advanced Genomics and Allied Techniques written by Aruna Pal and published by Springer Nature. This book was released on 2021-11-14 with total page 762 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.

Protein Blotting and Detection

Author : Biji T. Kurien
Publisher : Humana Press
ISBN 13 : 9781934115732
Total Pages : 588 pages
Book Rating : 4.1/5 (157 download)

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Book Synopsis Protein Blotting and Detection by : Biji T. Kurien

Download or read book Protein Blotting and Detection written by Biji T. Kurien and published by Humana Press. This book was released on 2009-05-15 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols", expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.

Diagnostic and Therapeutic Antibodies

Author : Andrew J.T. George
Publisher : Springer Science & Business Media
ISBN 13 : 1592590764
Total Pages : 471 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Diagnostic and Therapeutic Antibodies by : Andrew J.T. George

Download or read book Diagnostic and Therapeutic Antibodies written by Andrew J.T. George and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soon after the first description of monoclonal antibodies in 1976, there was enormous interest in the clinical application of antibodies, especially in the context of cancer. Antibodies appeared to offer the “magic bullet” that would allow the specific destruction of neoplastic cells. H- ever, many years’ effort resulted in very few cases of successful immu- therapy with antibodies. As a result there was a major backlash against antibody therapy, and the field lost a considerable amount of popularity. Fashion, in science as well as in other things, tends to be cyclical. Antibody-based therapy is once again attracting scientists and clinicians. There are several reasons for the renewed optimism; certainly the expe- ence of the last two decades has provided a wealth of information about problems associated with antibody therapy, and possible solutions to these problems. Recombinant antibody engineering has rejuvenated the field, allowing both the modification of antibodies to improve their in vivo pr- erties and the isolation of novel antibody molecules by such techniques as phage display. The results of recent clinical trials have demonstrated unequivocally the benefit of antibody therapy in a number of settings, and, finally, more careful consideration has been taken of the types of disease best treated using this approach.

ELISA

Author : Robert Hnasko
Publisher : Humana
ISBN 13 : 9781493953851
Total Pages : 0 pages
Book Rating : 4.9/5 (538 download)

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Book Synopsis ELISA by : Robert Hnasko

Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2016-10-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.

Basic Science Methods for Clinical Researchers

Author : Morteza Jalali
Publisher : Academic Press
ISBN 13 : 012803078X
Total Pages : 382 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Basic Science Methods for Clinical Researchers by : Morteza Jalali

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Malaria Methods and Protocols

Author : Denise L. Doolan
Publisher : Springer Science & Business Media
ISBN 13 : 1592592716
Total Pages : 606 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Malaria Methods and Protocols by : Denise L. Doolan

Download or read book Malaria Methods and Protocols written by Denise L. Doolan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.

Molecular Biology Techniques

Author : Walt Ream
Publisher : Academic Press
ISBN 13 : 0080536824
Total Pages : 245 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Molecular Biology Techniques by : Walt Ream

Download or read book Molecular Biology Techniques written by Walt Ream and published by Academic Press. This book was released on 1998-11-17 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter

Biomedical Image Analysis Recipes in MATLAB

Author : Constantino Carlos Reyes-Aldasoro
Publisher : John Wiley & Sons
ISBN 13 : 1118657551
Total Pages : 416 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Biomedical Image Analysis Recipes in MATLAB by : Constantino Carlos Reyes-Aldasoro

Download or read book Biomedical Image Analysis Recipes in MATLAB written by Constantino Carlos Reyes-Aldasoro and published by John Wiley & Sons. This book was released on 2015-06-22 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: As its title suggests, this innovative book has been written for life scientists needing to analyse their data sets, and programmers, wanting a better understanding of the types of experimental images life scientists investigate on a regular basis. Each chapter presents one self-contained biomedical experiment to be analysed. Part I of the book presents its two basic ingredients: essential concepts of image analysis and Matlab. In Part II, algorithms and techniques are shown as series of "recipes" or solved examples that show how specific techniques are applied to a biomedical experiments like Western Blots, Histology, Scratch Wound Assays and Fluoresence. Each recipe begins with simple techniques that gradually advance in complexity. Part III presents some advanced techniques for the generation of publication quality figures. The book does not assume any computational or mathematical expertise. A practical, clearly-written introduction to biomedical image analysis that provides the tools for life scientists and engineers to use when solving problems in their own laboratories. Presents the basic concepts of MATLAB software and uses it throughout to show how it can execute flexible and powerful image analysis programs tailored to the specific needs of the problem. Within the context of four biomedical cases, it shows algorithms and techniques as series of "recipes", or solved examples that show how a particular technique is applied in a specific experiment. Companion website containing example datasets, MATLAB files and figures from the book.

Essentials of Glycobiology

Author : Ajit Varki
Publisher : CSHL Press
ISBN 13 : 9780879696818
Total Pages : 694 pages
Book Rating : 4.6/5 (968 download)

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Book Synopsis Essentials of Glycobiology by : Ajit Varki

Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.

Breast Cancer Research Protocols

Author : Susan A. Brooks
Publisher : Springer Science & Business Media
ISBN 13 : 1592599699
Total Pages : 507 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Breast Cancer Research Protocols by : Susan A. Brooks

Download or read book Breast Cancer Research Protocols written by Susan A. Brooks and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes.

Introduction to Basics of Pharmacology and Toxicology

Author : Gerard Marshall Raj
Publisher : Springer Nature
ISBN 13 : 9813297794
Total Pages : 410 pages
Book Rating : 4.8/5 (132 download)

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Book Synopsis Introduction to Basics of Pharmacology and Toxicology by : Gerard Marshall Raj

Download or read book Introduction to Basics of Pharmacology and Toxicology written by Gerard Marshall Raj and published by Springer Nature. This book was released on 2019-11-16 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates, in a comprehensive manner, the most crucial principles involved in pharmacology and allied sciences. The title begins by discussing the historical aspects of drug discovery, with up to date knowledge on Nobel Laureates in pharmacology and their significant discoveries. It then examines the general pharmacological principles - pharmacokinetics and pharmacodynamics, with in-depth information on drug transporters and interactions. In the remaining chapters, the book covers a definitive collection of topics containing essential information on the basic principles of pharmacology and how they are employed for the treatment of diseases. Readers will learn about special topics in pharmacology that are hard to find elsewhere, including issues related to environmental toxicology and the latest information on drug poisoning and treatment, analytical toxicology, toxicovigilance, and the use of molecular biology techniques in pharmacology. The book offers a valuable resource for researchers in the fields of pharmacology and toxicology, as well as students pursuing a degree in or with an interest in pharmacology.

Enzyme-Mediated Immunoassay

Author : H.M. Lenhoff
Publisher : Springer Science & Business Media
ISBN 13 : 1468450123
Total Pages : 481 pages
Book Rating : 4.4/5 (684 download)

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Book Synopsis Enzyme-Mediated Immunoassay by : H.M. Lenhoff

Download or read book Enzyme-Mediated Immunoassay written by H.M. Lenhoff and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.

Guide to Protein Purification

Author : Richard R Burgess
Publisher : Academic Press
ISBN 13 : 0080923178
Total Pages : 915 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Guide to Protein Purification by : Richard R Burgess

Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Nanotoxicity

Author : Joshua Reineke
Publisher : Humana Press
ISBN 13 : 9781627030014
Total Pages : 0 pages
Book Rating : 4.0/5 (3 download)

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Book Synopsis Nanotoxicity by : Joshua Reineke

Download or read book Nanotoxicity written by Joshua Reineke and published by Humana Press. This book was released on 2012-09-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of nanotechnology has developed very rapidly over the past decade lending great promise to medical applications in drug delivery, therapeutics, and biological imaging. Due to the great promise, rapid development, and broad application of nanomaterials, it is imperative that researchers from development through application seek a thorough understanding of nanotoxicity. Nanotoxicity: Methods and Protocols address the special considerations when applying toxicity studies to nanomaterials and detail newly developed methods for the study of nanotoxicity. These diverse methods span in vitro cell culture, model tissues, in situ exposure, in vivo models, analysis in plants, and mathematical modeling, proving to be relevant to pharmaceutical scientists, material scientists, bioengineers, toxicologists, environmentalists, immunologists, and cellular and molecular biologists, to name a few. As part of the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Nanotoxicity: Methods and Protocols aims to diversify the capabilities of current researchers involved in nanotoxicology and to enable researchers in related fields to expand their knowledge of how nanomaterials interface with the biological environment.