Protein Purification

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Publisher : Springer Science & Business Media
ISBN 13 : 1475719574
Total Pages : 342 pages
Book Rating : 4.4/5 (757 download)

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Book Synopsis Protein Purification by : Robert K. Scopes

Download or read book Protein Purification written by Robert K. Scopes and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.

Electrophoretic Separation of Proteins

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Publisher :
ISBN 13 : 9781493987931
Total Pages : 523 pages
Book Rating : 4.9/5 (879 download)

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Book Synopsis Electrophoretic Separation of Proteins by : Biji T. Kurien

Download or read book Electrophoretic Separation of Proteins written by Biji T. Kurien and published by . This book was released on with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Membrane Protein Purification and Crystallization

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Publisher : Elsevier
ISBN 13 : 0080536174
Total Pages : 339 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Membrane Protein Purification and Crystallization by : Carola Hunte

Download or read book Membrane Protein Purification and Crystallization written by Carola Hunte and published by Elsevier. This book was released on 2003-01-05 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. - Provides general guidelines and strategies for isolation and crystallization of membrane proteins - Gives detailed protocols that have wide application, and low specialized equipment needs - Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins - Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes - Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization - Presents a comprehensive guide to 2D-crystallization of membrane proteins

Molecular Biology of the Cell

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Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Advanced Dairy Chemistry: Volume 1: Proteins, Parts A&B

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Publisher : Springer
ISBN 13 : 1441986022
Total Pages : 1356 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Advanced Dairy Chemistry: Volume 1: Proteins, Parts A&B by : Patrick F. Fox

Download or read book Advanced Dairy Chemistry: Volume 1: Proteins, Parts A&B written by Patrick F. Fox and published by Springer. This book was released on 2013-09-24 with total page 1356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Dairy Chemistry-l: Proteins is the first volume of the third edition of the series on advanced topics in Dairy Chemistry, which started in 1982 with the publication of Developments in Dairy Chemistry. This series of volume~ is intended to be a coordinated and authoritative treatise on Dairy Chemistry. In the decade since the second edition of this volume was published (1992), there have been considerable advances in the study of milk proteins, which are reflected in changes to this book. All topics included in the second edition are retained in the current edition, which has been updated and considerably expanded from 18 to 29 chapters. Owing to its size, the book is divided into two parts; Part A (Chapters 1-11) describes the more basic aspects of milk proteins while Part B (Chapters 12-29) reviews the more applied aspects. Chapter 1, a new chapter, presents an overview of the milk protein system, especially from an historical viewpoint. Chapters 2-5, 7-9, 15, and 16 are revisions of chapters in the second edition and cover analytical aspects, chemical and physiochemical properties, biosynthesis and genetic polymorphism of the principal milk proteins. Non-bovine caseins are reviewed in Chapter 6.

Engineering Processes for Bioseparations

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Publisher : Elsevier
ISBN 13 : 148329210X
Total Pages : 305 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Engineering Processes for Bioseparations by : LAURENCE R. WEATHERLEY

Download or read book Engineering Processes for Bioseparations written by LAURENCE R. WEATHERLEY and published by Elsevier. This book was released on 2013-10-22 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of biotechnology in chemical synthesis offers up numerous advantages to the engineer in the process industries, but it also presents a number of fundamental challenges and difficulties which impinge directly on separation process requirements. The use of biochemical separations has grown significantly during the past decade, and is especially used in process industries such as healthcare and food processing. However it is becoming increasingly more important in areas such as recycling and waste-water treatment and as industry shifts towards cleaner processes biochemical separations will continue to grow. The two main objectives of this book are to focus on the application of existing separation process techniques to the recovery and purification of biologically derived products and to examine the state of knowledge of new techniques which have future potential. Within these objectives the complexities and breadth of problems associated with biological separations are discussed, specific engineering techniques are featured and their adaptation to biochemical separations are highlighted.

Isolation and Purification of Proteins

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Publisher : CRC Press
ISBN 13 : 0824747593
Total Pages : 653 pages
Book Rating : 4.8/5 (247 download)

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Book Synopsis Isolation and Purification of Proteins by : Rajni Hatti-Kaul

Download or read book Isolation and Purification of Proteins written by Rajni Hatti-Kaul and published by CRC Press. This book was released on 2003-02-05 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Principles and Reactions of Protein Extraction, Purification, and Characterization

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Publisher : CRC Press
ISBN 13 : 0203507436
Total Pages : 408 pages
Book Rating : 4.2/5 (35 download)

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Book Synopsis Principles and Reactions of Protein Extraction, Purification, and Characterization by : Hafiz Ahmed

Download or read book Principles and Reactions of Protein Extraction, Purification, and Characterization written by Hafiz Ahmed and published by CRC Press. This book was released on 2017-07-27 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different

A Guide to Protein Isolation

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Publisher : Springer Science & Business Media
ISBN 13 : 9401702691
Total Pages : 266 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis A Guide to Protein Isolation by : C. Dennison

Download or read book A Guide to Protein Isolation written by C. Dennison and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of "fundamentalness" appropriate to the task in hand. For example, an in-depth study of the mechanics of motor cars would tell one nothing about the dynamics of traffic. Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible.

A Laboratory Manual of Analytical Methods of Protein Chemistry

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Publisher : Elsevier
ISBN 13 : 1483152456
Total Pages : 265 pages
Book Rating : 4.4/5 (831 download)

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Book Synopsis A Laboratory Manual of Analytical Methods of Protein Chemistry by : P. Alexander

Download or read book A Laboratory Manual of Analytical Methods of Protein Chemistry written by P. Alexander and published by Elsevier. This book was released on 2014-05-17 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 5 presents the laboratory techniques for protein and polypeptide study. This book discusses the staining procedure for histones, which has a high degree of selectivity for basic proteins and the unique ability to visualize qualitative differences in terms of color changes. Organized into four chapters, this volume begins with an overview of the formalin-mediated ammoniacal-silver staining procedure as a selective stain for basic proteins and its application per cell and per extract. This text then examines the optical rotatory dispersion (ORD), which has advanced into a powerful tool for describing the conformations and conformational changes of biopolymers. Other chapters consider the application of ultrasensitive calorimetry to thermodynamic problems. This book discusses as well the principle of the technique, its instrumentation, and experimental procedures. The final chapter deals with the hydrodynamic densities and preferential hydration values for protein precipitates in concentrated salt solutions. This book is a valuable resource for chemists and biochemists.

Encyclopedia of Neuroscience, Volume 1

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Publisher : Academic Press
ISBN 13 : 0080963935
Total Pages : 12505 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Encyclopedia of Neuroscience, Volume 1 by : Larry R. Squire

Download or read book Encyclopedia of Neuroscience, Volume 1 written by Larry R. Squire and published by Academic Press. This book was released on 2009-06-12 with total page 12505 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia of the Neuroscience explores all areas of the discipline in its focused entries on a wide variety of topics in neurology, neurosurgery, psychiatry and other related areas of neuroscience. Each article is written by an expert in that specific domain and peer reviewed by the advisory board before acceptance into the encyclopedia. Each article contains a glossary, introduction, a reference section, and cross-references to other related encyclopedia articles. Written at a level suitable for university undergraduates, the breadth and depth of coverage will appeal beyond undergraduates to professionals and academics in related fields.

Methods in Membrane Biology

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Publisher : Springer Science & Business Media
ISBN 13 : 1461574226
Total Pages : 290 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Methods in Membrane Biology by : Edward D. Korn

Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examination of the tables of contents of journals - biochemical, molecular biological, ultrastructural, and physiological-provides convincing evidence that membrane biology will be in the 1970s what biochemical genetics was in the 1960s. And for good reason. If genetics is the mechanism for main taining and transmitting the essentials of life, membranes are in many ways the essence of life. The minimal requirement for independent existence is the individualism provided by the separation of "life" from the environment. The cell exists by virtue of its surface membran~. One might define the first living organism as that stage of evolution where macromolecular catalysts or self-reproducing polymers were first segregated from the surrounding milieu by a membrane. Whether that early membrane resembled present cell membranes is irrelevant. What matters is that a membrane would have provided a mechanism for maintaining a local concentration of molecules, facilitating chemical evolution and allowing it to evolve into biochemical evolution. That or yet more primitive membranes, such as a hydrocarbon monolayer at an air-water interface, could also have provided a surface that would facilitate the aggregation and specific orientation of molecules and catalyze their interactions. If primitive membranes were much more than mere passive barriers to free diffusion, how much more is this true of the membranes of contemporary forms of life. A major revolution in biological thought has been the recogni tion that the cell, and especially the eukaryotic cell, is a bewildering maze of membranes and membranous organelles.

Solid-Phase Extraction

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Publisher : Elsevier
ISBN 13 : 0128169079
Total Pages : 742 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Solid-Phase Extraction by : Colin F. Poole

Download or read book Solid-Phase Extraction written by Colin F. Poole and published by Elsevier. This book was released on 2019-09-12 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid Phase Extraction thoroughly presents both new and historic techniques for dealing with solid phase extraction. It provides all information laboratory scientists need for choosing and utilizing suitable sample preparation procedures for any kind of sample. In addition, the book showcases the contemporary uses of sample preparation techniques in the most important industrial and academic project environments, including solid-phase Microextraction, molecularly imprinted polymers, magnetic nanoparticles, and more. Written by recognized experts in their respective fields, this one-stop reference is ideal for those who need to know which technique to choose for solid phase extraction. Used in conjunction with a similar release, Liquid Phase Extraction, this book allows users to master this crucial aspect of sample preparation. - Defines the current state-of-the-art in extraction techniques and the methods and procedures for implementing them in laboratory practice - Includes extensive referencing that facilitates the identification of key information - Aimed at both entry-level scientists and those who want to explore new techniques and methods

Bioseparation and Bioprocessing, Volume I: Biochromatography - Membrane Separations - Modeling - Validation. Volume II: Processing - Quality and Characterisation - Economics, Safety and Hygiene

Download Bioseparation and Bioprocessing, Volume I: Biochromatography - Membrane Separations - Modeling - Validation. Volume II: Processing - Quality and Characterisation - Economics, Safety and Hygiene PDF Online Free

Author :
Publisher : Wiley-VCH
ISBN 13 :
Total Pages : 568 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Bioseparation and Bioprocessing, Volume I: Biochromatography - Membrane Separations - Modeling - Validation. Volume II: Processing - Quality and Characterisation - Economics, Safety and Hygiene by : G. Subramanian

Download or read book Bioseparation and Bioprocessing, Volume I: Biochromatography - Membrane Separations - Modeling - Validation. Volume II: Processing - Quality and Characterisation - Economics, Safety and Hygiene written by G. Subramanian and published by Wiley-VCH. This book was released on 1998 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid developments in biotechnology create a demand for practical, up-to-date reviews written by and for experts in industry. This compact handbook provides all relevant up-to-date information on important bioseparation and bioprocessing techniques that are actively applied in the biotechnology industries. The handbook presents an applications-orientated overview on - case studies and general strategies for quality control and characterization - detailed guidelines on developing economic and technically feasible bioseparation schemes - strategies and methods for intracellular bioproduct release - chromatographic and membrane downstream processes used in biotechnology - applications of modern non-invasive methods such as neural networks for on-line estimation and control of fermentation variables on an industrial scale - a practical, commercially-relevant guide to biosafety and many more aspects which are indispensible for present and future industrial success.

Downstream Processing of Proteins

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Publisher : Springer Science & Business Media
ISBN 13 : 1592590276
Total Pages : 233 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Downstream Processing of Proteins by : Mohamed A. Desai

Download or read book Downstream Processing of Proteins written by Mohamed A. Desai and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process.

Information Sources in Chemistry

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Author :
Publisher : Walter de Gruyter
ISBN 13 : 3110976854
Total Pages : 360 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Information Sources in Chemistry by : R. T. Bottle

Download or read book Information Sources in Chemistry written by R. T. Bottle and published by Walter de Gruyter. This book was released on 2011-12-01 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of each volume of this series Guides to Information Sources is to reduce the time which needs to be spent on patient searching and to recommend the best starting point and sources most likely to yield the desired information. The criteria for selection provide a way into a subject to those new to the field and assists in identifying major new or possibly unexplored sources to those who already have some acquaintance with it. The series attempts to achieve evaluation through a careful selection of sources and through the comments provided on those sources.

The Biochemical Journal

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Publisher :
ISBN 13 :
Total Pages : 812 pages
Book Rating : 4.:/5 (334 download)

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Book Synopsis The Biochemical Journal by :

Download or read book The Biochemical Journal written by and published by . This book was released on 1963 with total page 812 pages. Available in PDF, EPUB and Kindle. Book excerpt: