Unnatural Amino Acid Incorporation And Click Chemistry

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Unnatural Amino Acid Incorporation and Click Chemistry

Author : Anonym
Publisher :
ISBN 13 : 9783668663473
Total Pages : 54 pages
Book Rating : 4.6/5 (634 download)

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Book Synopsis Unnatural Amino Acid Incorporation and Click Chemistry by : Anonym

Download or read book Unnatural Amino Acid Incorporation and Click Chemistry written by Anonym and published by . This book was released on 2018-03-21 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: Seminar paper from the year 2014 in the subject Chemistry - Bio-chemistry, grade: 1,0, LMU Munich (Chemie), language: English, abstract: In the present work a modified version of yellow fluorescent protein containing an unnatural structural homologue of the natural amino acid pyrrolysine with a norbornene moiety was produced by expression in Escherichia coli. The incorporation of the unnatural amino acid was achieved by amber stop codon suppression method. A bio-othogonal click reaction was performed, binding a synthetic fluorescent dye to the modified protein. All steps towards necessary for obtaining the genetically modified organism were performed and documented. The artificial amino acid, as well as the dye used in the click reaction were synthetically prepared. The success of the project was demonstrated by LC/MS studies of the products. Fluorescence spectroscopy of click reaction product and the protein was performed, but no conclusive proof of FRET effects could as yet be made. This point remains of interest for future studies.

Non-Natural Amino Acids

Author :
Publisher : Academic Press
ISBN 13 : 0080921639
Total Pages : 334 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Non-Natural Amino Acids by :

Download or read book Non-Natural Amino Acids written by and published by Academic Press. This book was released on 2009-07-24 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Synthesis of a Novel Unnatural Amino Acid for Protein Incorporation and Click Mediated Conjugation

Author : Christopher A. Farley
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (15 download)

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Book Synopsis Synthesis of a Novel Unnatural Amino Acid for Protein Incorporation and Click Mediated Conjugation by : Christopher A. Farley

Download or read book Synthesis of a Novel Unnatural Amino Acid for Protein Incorporation and Click Mediated Conjugation written by Christopher A. Farley and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Unnatural amino acids (UAAs) contain side chains, or R groups, that are not found in the 20 canonical amino acids. These noncanonical groups afford the capability to incorporate powerful chemical capabilities in proteins that are ordinarily unavailable with the naturally-occurring amino acids. Among the most useful moieties to incorporate into proteins are functional groups that can undergo Huisgen [3+2] cycloadditions, or “click,” reactions. This reaction occurs between azides and alkynes, and its mild conditions and high regioselectivity and reactivity make it an ideal process for bioconjugation. Photoreactivity is another useful characteristic that can be conferred to UAAs. Photolabile caging groups can inhibit the function of a protein until brief irradiation with UV light induces an intramolecular rearrangement and its displacement, reestablishing normal function. In this thesis, we propose a synthesis to incorporate both of these moieties into a single UAA.

Unnatural Amino Acids Designed for Click Chemistry and Their Application to the Modification of Peptides & Nitrene Transfer Reactions Catalyzed by Metalloporphyrins

Download Unnatural Amino Acids Designed for Click Chemistry and Their Application to the Modification of Peptides & Nitrene Transfer Reactions Catalyzed by Metalloporphyrins PDF Online Free

Author :
Publisher :
ISBN 13 :
Total Pages : 223 pages
Book Rating : 4.:/5 (466 download)

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Book Synopsis Unnatural Amino Acids Designed for Click Chemistry and Their Application to the Modification of Peptides & Nitrene Transfer Reactions Catalyzed by Metalloporphyrins by :

Download or read book Unnatural Amino Acids Designed for Click Chemistry and Their Application to the Modification of Peptides & Nitrene Transfer Reactions Catalyzed by Metalloporphyrins written by and published by . This book was released on 2009 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of peptidomimetics has rapidly grown into an area of great interest for the design and synthesis of pharmaceutical drug targets. The large array of natural peptides with biological function as well as the growing understanding of the roles of these peptides in biological events has led to a large interest in these compounds as drug candidates. The majority of peptide and peptide-like molecules have not found widespread pharmaceutical utility; however, due to there lability in biological systems. This major drawback leads to the necessity for the development of peptide-like molecules with increased stability under biological conditions. To this end there has been an increased interest in the development of unnatural amino acids for the synthetic modification of peptides and proteins. Presented in this dissertation is the synthesis of a series of unnatural amino acids designed for applications to [3+2] click cycloaddition reactions. It also covers the introduction of these amino acids into the sequence of peptides for the purpose of labeling the peptide with aryl triazole chromophores in an attempt to analyze the electron transfer capabilities of aryl triazoles. The information from the fluorescent studies of these peptides will provide a basis for the design of fluorophoric peptide probes that can be introduced into a peptide at any time under labile conditions. This methodology provides a powerful tool for the analysis of peptide structure and the analysis of peptide-macromolecular interactions.

Peptide, Protein and Enzyme Design

Author :
Publisher : Academic Press
ISBN 13 : 0128054344
Total Pages : 686 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Peptide, Protein and Enzyme Design by :

Download or read book Peptide, Protein and Enzyme Design written by and published by Academic Press. This book was released on 2016-08-27 with total page 686 pages. Available in PDF, EPUB and Kindle. Book excerpt: De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. Continues the legacy of this premier serial with quality chapters on de novo enzyme design Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism

Micro and Nanofabrication Using Self-Assembled Biological Nanostructures

Author : Jaime Castillo-León
Publisher : William Andrew
ISBN 13 : 9780323296427
Total Pages : 0 pages
Book Rating : 4.2/5 (964 download)

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Book Synopsis Micro and Nanofabrication Using Self-Assembled Biological Nanostructures by : Jaime Castillo-León

Download or read book Micro and Nanofabrication Using Self-Assembled Biological Nanostructures written by Jaime Castillo-León and published by William Andrew. This book was released on 2014-09-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Self-assembled nanostructures based on peptides and proteins have been investigated and presented as biomaterials with an impressive potential for a broad range of applications such as microfabrication, biosensing platforms, drug delivery systems, bioelectronics and tissue reparation. Through self-assembly peptides can give rise to a range of well-defined nanostructures such as nanotubes, nanofibers, nanoparticles, nanotapes, gels and nanorods. However, there are challenges when trying to integrate these biological nanostructures in the development of sensing devices or drug-delivery systems - challenges such as controlling the size during synthesis, the stability in liquid environments and manipulation. In "Micro and Nanofabrication Using Self-assembled Biological Nanostructures" the options and challenges when using self-assembled peptide nanostructures in micro and nanofabrication are discussed. The publication covers different ways to manipulate, deposit and immobilize on specific locations these biological nanostructures in order to use them in the fabrication of new structures or as part of biosensing platforms. Examples where researchers used biological nanostructures for those types of applications are provided. Finally, future applications are discussed as well as parameters to accelerate and expand the use of these biological building blocks in nano- and micro-fabrication processes by taking advantage of their impressive properties such as low-cost and short synthesis time.

Bioconjugate Techniques

Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 : 0080568726
Total Pages : 1233 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Bioconjugate Techniques by : Greg T. Hermanson

Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

Unnatural Amino Acids

Author : Loredano Pollegioni
Publisher : Humana Press
ISBN 13 : 9781617793301
Total Pages : 409 pages
Book Rating : 4.7/5 (933 download)

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Book Synopsis Unnatural Amino Acids by : Loredano Pollegioni

Download or read book Unnatural Amino Acids written by Loredano Pollegioni and published by Humana Press. This book was released on 2011-10-05 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of unnatural amino acids in peptides with antimicrobial properties, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.

Fluorine-containing Amino Acids

Author : Valery P. Kukhar
Publisher : John Wiley & Sons
ISBN 13 :
Total Pages : 432 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Fluorine-containing Amino Acids by : Valery P. Kukhar

Download or read book Fluorine-containing Amino Acids written by Valery P. Kukhar and published by John Wiley & Sons. This book was released on 1995-04-06 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, organo-fluorine chemistry has made a marked impact on the design and synthesis of a large variety of biologically active molecules, such as steroids, carbohydrates, amines, amino acids, peptides and other natural products. Naturally occurring amino acids play a pivotal role in living systems, and therefore synthetic fluorine-containing amino acids have been of significant interest to researchers working towards the understanding and modification of physiological processes. Fluorine-containing Amino Acids: is the first volume devoted to the synthesis and properties of fluorine-containing amino acids pays special attention to the preparation of enantiomerically pure acids (which are essential to the modern pharmaceutical industry) deals with a rapidly expanding field of research has been written by experienced researchers who are responsible for many developments in the field highlights the interdisciplinary nature of this topic Fluorine-containing Amino Acids is the only dedicated reference in this subject and will be essential for researchers in synthetic organic, peptide, natural product, and medicinal chemistry and biochemistry.

Click Triazoles

Author : Janez Košmrlj
Publisher : Springer
ISBN 13 : 3642294294
Total Pages : 245 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis Click Triazoles by : Janez Košmrlj

Download or read book Click Triazoles written by Janez Košmrlj and published by Springer. This book was released on 2012-05-30 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: B. R. Buckley and H. Heaney: Mechanistic Investigations of Copper(I)- Catalyzed Alkyne–Azide Cycloaddition Reactions.- J. D. Crowley and D. A. McMorran: “Click-Triazole” Coordination Chemistry: Exploiting 1,4-Disubstituted-1,2,3-Triazoles as Ligands.- S. Lee and A. H. Flood: Binding Anions in Rigid and Reconfigurable Triazole Receptors.- M. Watkinson: Click Triazoles as Chemosensors.- H.-F. Chow, C.-M. Lo and Y. Chen: Triazole-Based Polymer Gels.- T. Zheng, S. H. Rouhanifard, A. S. Jalloh, P. Wu: Click Triazoles for Bioconjugation.- S. Mignani, Y. Zhou, T. Lecourt and L. Micouin: Recent Developments in the Synthesis 1,4,5-Trisubstituted Triazoles.

A General Strategy for Site-specific Incorporation of Unnatural Amino Acids Into Proteins

Author : Spencer Jay Anthony-Cahill
Publisher :
ISBN 13 :
Total Pages : 676 pages
Book Rating : 4.:/5 (33 download)

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Book Synopsis A General Strategy for Site-specific Incorporation of Unnatural Amino Acids Into Proteins by : Spencer Jay Anthony-Cahill

Download or read book A General Strategy for Site-specific Incorporation of Unnatural Amino Acids Into Proteins written by Spencer Jay Anthony-Cahill and published by . This book was released on 1990 with total page 676 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Noncanonical Amino Acids

Author : Edward A. Lemke
Publisher :
ISBN 13 : 9781493975747
Total Pages : 411 pages
Book Rating : 4.9/5 (757 download)

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Book Synopsis Noncanonical Amino Acids by : Edward A. Lemke

Download or read book Noncanonical Amino Acids written by Edward A. Lemke and published by . This book was released on 2018 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Engineering the Genetic Code

Author : Nediljko Budisa
Publisher : John Wiley & Sons
ISBN 13 : 3527607099
Total Pages : 312 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Engineering the Genetic Code by : Nediljko Budisa

Download or read book Engineering the Genetic Code written by Nediljko Budisa and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.

Mass Spectrometry-Based Chemical Proteomics

Author : W. Andy Tao
Publisher : John Wiley & Sons
ISBN 13 : 1118970217
Total Pages : 448 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao

Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Life Sciences Industry

Author : Basanta Kumara Bahera
Publisher : Springer Nature
ISBN 13 : 9811620512
Total Pages : 221 pages
Book Rating : 4.8/5 (116 download)

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Book Synopsis Life Sciences Industry by : Basanta Kumara Bahera

Download or read book Life Sciences Industry written by Basanta Kumara Bahera and published by Springer Nature. This book was released on 2021-08-24 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic principles of applied life sciences such as recombinant DNA technology is used in most life sciences industries marketing bio-formulations for designing more effective protein-based drugs, such as erythropoietin and fast-acting insulin etc. In recent times genetically engineered host cells from mammal, animal and plants are also being used in life sciences industries to manufacture biologics. This book discusses the most basic as well advanced issues on biological products for successfully managing a life sciences industry. It elucidates the life cycle of biological molecules, right from the conceptual development of different types of biopolymers, and their subsequent transfer from the conical flasks in laboratory to life sciences industries for large scale production and marketing. It focuses on sustainable longevity in the life cycle of commercial biopolymers. Cumulative facts and figures in this volume would immensely help in inspiring life sciences industry promoters to monitor value chain transfer process of biologics for better profitability. Additionally, it would serve as a perusal document for the students and researchers interested in entrepreneurial ventures or having their own start-up projects for the commercialization of biologics.

Fluorescent Analogs of Biomolecular Building Blocks

Author : Marcus Wilhelmsson
Publisher : John Wiley & Sons
ISBN 13 : 1118175867
Total Pages : 474 pages
Book Rating : 4.1/5 (181 download)

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Book Synopsis Fluorescent Analogs of Biomolecular Building Blocks by : Marcus Wilhelmsson

Download or read book Fluorescent Analogs of Biomolecular Building Blocks written by Marcus Wilhelmsson and published by John Wiley & Sons. This book was released on 2016-04-04 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.

Nitroxides

Author : Olivier Ouari
Publisher : Royal Society of Chemistry
ISBN 13 : 1788019660
Total Pages : 611 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Nitroxides by : Olivier Ouari

Download or read book Nitroxides written by Olivier Ouari and published by Royal Society of Chemistry. This book was released on 2021-05-13 with total page 611 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nitroxides are versatile small organic molecules possessing a stabilised free radical. With their unpaired electron spin they display a unique reactivity towards various environmental factors, enabling a diverse range of applications. They have uses as synthetic tools, such as catalysts or building blocks; imaging agents and probes in biomedicine and materials science; for medicinal antioxidant applications; and in energy storage. Polynitroxides (polymers bearing pendant nitroxide sidechains) have been used in organic radical batteries, oxidation catalysts and in exchange reactions for constructing complex architectures. Chapters in this book cover the synthesis of nitroxides, EPR studies and magnetic resonance applications, physiochemical studies, and applications including in batteries, imaging and organic synthesis. With contributions from leaders in the field, Nitroxides will be of interest to graduate students and researchers across chemistry, physics, biology and materials science.