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Time Resolved Femtosecond Optical Studies Of Heme Proteins Myoglobin And Cytochrome C
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Book Synopsis Time Resolved Femtosecond Optical Studies of Heme Proteins Myoglobin and Cytochrome C by : Jeffrey Alan Stevens
Download or read book Time Resolved Femtosecond Optical Studies of Heme Proteins Myoglobin and Cytochrome C written by Jeffrey Alan Stevens and published by . This book was released on 2011 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Our goal was to develop methods to study protein dynamics on the picosecond to nanosecond time scale. We used wavelength and femtosecond resolved fluorescence and absorbance spectroscopy coupled with site-directed mutagenesis to study the heme proteins myoglobin and cytochrome c and determine protein dynamics occurring on the picosecond to nanosecond time scale. The intrinsic amino acid tryptophan was used as a local optical probe and placed one at a time with site-directed mutagenesis that allowed us to achieve a spatial resolution down to a single amino acid. We systematically characterized resonance energy transfer (RET) between a single tryptophan and the prosthetic heme group in myoglobin to develop a novel energy-transfer pair as a molecular ruler in heme proteins to study local conformation fluctuations. Using site-directed mutagenesis, we placed a single tryptophan throughout myoglobin. We observed picosecond RET in tens to hundreds of picoseconds. We also characterized steady state parameters associated with RET allowing us to determine the orientation factor between tryptophan and heme and thus examine changes at different mutation sites revealing relative local structure flexibility and conformation fluctuations. Additionally, local environment relaxation occurs on similar time scales as the RET dynamics, resulting in a nonequilibrium dynamic process. We were able to determine the time scales of such nonequilibrium energy-transfer dynamics and elucidate the mechanism of the nonexponential RET dynamics caused by local dynamic heterogeneity and/or local environment relaxation. In cytochrome c, RET between the lone intrinsic tryptophan and heme was used to study protein folding/unfolding for various states (ferric, ferrous and CO-bound ferrous) of cytochrome c. We systematic studied heme dynamics and induced protein conformational relaxations in cytochrome c and myoglobin with femtosecond transient absorption (TA). A wide range of probing wavelengths from the visible to the UV was probed to unambiguously determine the protein dynamics of the ferric and ferrous state of cytochrome c. For cytochrome c in the ferrous state, the heme undergoes ligand bond breaking and rebinding process generating proteinquakes that perturb the local heme site and shake global protein conformation which was detected by the nearby lone intrinsic tryptophan. Cytochrome c in the ferric state has dynamics that mainly occur at the local site, including ultrafast internal conversion, vibrational cooling, and complete ground-state recovery with no global conformation relaxation observed. We expanded our studies by coupling site-directed mutagenesis of tryptophan at various locations throughout cytochrome c utilizing TA studies. Ligand photolysis from heme was used to generate proteinquakes perturbing the local heme site and shakes global protein conformation and was observed by several of the tryptophan mutations. Additionally, we observed the protein dynamics as a function of denaturing agent. The region that we studied was the pre-unfolding transition region; hence the protein was "softened." In myoglobin, we similarly characterized the heme dynamics of the ferric, ferrous and CO-bound ferrous states. We attempted to use tryptophan as a local probe of protein dynamics for CO-bound myoglobin, but concluded that the perturbation created by CO photolysis was much smaller compared to the proteinquake generated in cytochrome c.
Book Synopsis Gregory S. Ezra by : Srihari Keshavamurthy
Download or read book Gregory S. Ezra written by Srihari Keshavamurthy and published by Springer. This book was released on 2015-06-01 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this Festschrift dedicated to the 60th birthday of Gregory S. Ezra, selected researchers in theoretical chemistry present research highlights on major developments in the field. Originally published in the journal Theoretical Chemistry Accounts, these outstanding contributions are now available in a hardcover print format, as well as a special electronic edition. This volume provides valuable content for all researchers in theoretical chemistry and will especially benefit those research groups and libraries with limited access to the journal.
Book Synopsis Elucidation of Various Mechanistic Aspects of Cytochrome C Oxidase Using Time-resolved Optical Absorption Spectroscopy by : Indranil Sen
Download or read book Elucidation of Various Mechanistic Aspects of Cytochrome C Oxidase Using Time-resolved Optical Absorption Spectroscopy written by Indranil Sen and published by . This book was released on 2009 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Equilibrium and Time-resolved Spectroscopic Studies on Cytochrome C and Other Selected Proteins by : Qi Xu
Download or read book Equilibrium and Time-resolved Spectroscopic Studies on Cytochrome C and Other Selected Proteins written by Qi Xu and published by . This book was released on 2004 with total page 602 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Modern Optical Spectroscopy by : William W. Parson
Download or read book Modern Optical Spectroscopy written by William W. Parson and published by Springer Nature. This book was released on 2023-02-28 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 3rd edition of this textbook offers clear explanations of optical spectroscopic phenomena and shows how spectroscopic techniques are used in modern chemistry, biochemistry and biophysics. Topics included are: electronic and vibrational absorption fluorescence symmetry operations and normal-mode calculations electron transfer from excited molecules energy transfer exciton interactions electronic and vibrational circular dichroism coherence and dephasing ultrafast pump-probe and photon-echo spectroscopy single-molecule and fluorescence-correlation spectroscopy Raman scattering multiphoton absorption quantum optics and non-linear optics entropy changes during photoexcitation electronic and vibrational Stark effects studies of fast processes in single molecules two-dimensional electronic and vibrational spectroscopy This revised and updated edition provides expanded discussions of laser spectroscopy, crystal symmetry, birefringence, non-linear optics, solar cells and light-emitting diodes. The explanations are sufficiently thorough and detailed to be useful for researchers, graduate students and advanced undergraduates in chemistry, biochemistry and biophysics. They are based on time-dependent quantum mechanics, but are developed from first principles so that they can be understood by readers with little prior training in the field. Additional topics and highlights are presented in special boxes in the text. The book is richly illustrated with color figures throughout. Each chapter ends with a section of questions for self-examination.
Book Synopsis Hemes and Hemoproteins by : Johnson Research Foundation
Download or read book Hemes and Hemoproteins written by Johnson Research Foundation and published by . This book was released on 1966 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteins written by David M. Leitner and published by CRC Press. This book was released on 2009-09-28 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computational modeling can provide a wealth of insight into how energy flow in proteins mediates protein function. Computational methods can also address fundamental questions related to molecular signaling and energy flow in proteins. Proteins: Energy, Heat and Signal Flow presents state-of-the-art computational strategies for studying energy redi
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1988 with total page 1332 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Photosynthetic Reaction Center by : Johann Deisenhofer
Download or read book Photosynthetic Reaction Center written by Johann Deisenhofer and published by Academic Press. This book was released on 2012-12-02 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: The availability of the photosynthetic reaction center's structure at an atomic resolution of less than three angstroms has revolutionized research. This protein is the first integral membrane protein whose structure has been determined with such precision. Each volume of the Photosynthetic Reaction Center contains original research, methods, and reviews. Together, these volumes cover our current understanding of how photosynthesis converts light energy into stored chemical energy.Volume I describes the chemistry and biochemistry of photosynthesis, including green plant photosynthesis; it is devoted to the overall features and implications of the bacterial reaction center for green plant research. It features a new description of the structure of the reaction center, followed by coverage of the antenna and light functions. Volume I also details new manipulations of the reaction center including chemical and genetic modifications. It describes how the reaction center provides reducing power via electron transfer chemistry coupled to proton uptake and release; coupling of electron transport between the oxidized reaction center and the aqueous periplasm; and the general operation of membrane-bound proteins. Additionally, this volume contains five chapters detailing facets of green plant photosynthesis important for future research.
Book Synopsis Annual Review by : Bunshi Kagaku Kenkyūjo
Download or read book Annual Review written by Bunshi Kagaku Kenkyūjo and published by . This book was released on 2003 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2566 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :Janice M. Hicks Publisher :SPIE-International Society for Optical Engineering ISBN 13 : Total Pages :330 pages Book Rating :4.3/5 (91 download)
Book Synopsis Laser Techniques for Condensed-phase and Biological Systems by : Janice M. Hicks
Download or read book Laser Techniques for Condensed-phase and Biological Systems written by Janice M. Hicks and published by SPIE-International Society for Optical Engineering. This book was released on 1998 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Macromolecular Crystallography by : Charles W. Carter
Download or read book Macromolecular Crystallography written by Charles W. Carter and published by Gulf Professional Publishing. This book was released on 1997 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation Accurate molecular structures is vital for rational drug design and for structure based functional studies directed toward the development of effective therapeutic agents and drugs. Crystallography can reliably predict structure, both in terms of folding and atomic details of bonding. * Phases * Map interpretation and refinement * Analysis and software.
Download or read book Heme Peroxidases written by Emma Raven and published by Royal Society of Chemistry. This book was released on 2015-10-26 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2001 with total page 834 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Synchrotron Light Sources and Free-Electron Lasers by : Eberhard J. Jaeschke
Download or read book Synchrotron Light Sources and Free-Electron Lasers written by Eberhard J. Jaeschke and published by Springer. This book was released on 2016-05-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hardly any other discovery of the nineteenth century did have such an impact on science and technology as Wilhelm Conrad Röntgen’s seminal find of the X-rays. X-ray tubes soon made their way as excellent instruments for numerous applications in medicine, biology, materials science and testing, chemistry and public security. Developing new radiation sources with higher brilliance and much extended spectral range resulted in stunning developments like the electron synchrotron and electron storage ring and the freeelectron laser. This handbook highlights these developments in fifty chapters. The reader is given not only an inside view of exciting science areas but also of design concepts for the most advanced light sources. The theory of synchrotron radiation and of the freeelectron laser, design examples and the technology basis are presented. The handbook presents advanced concepts like seeding and harmonic generation, the booming field of Terahertz radiation sources and upcoming brilliant light sources driven by laser-plasma accelerators. The applications of the most advanced light sources and the advent of nanobeams and fully coherent x-rays allow experiments from which scientists in the past could not even dream. Examples are the diffraction with nanometer resolution, imaging with a full 3D reconstruction of the object from a diffraction pattern, measuring the disorder in liquids with high spatial and temporal resolution. The 20th century was dedicated to the development and improvement of synchrotron light sources with an ever ongoing increase of brilliance. With ultrahigh brilliance sources, the 21st century will be the century of x-ray lasers and their applications. Thus, we are already close to the dream of condensed matter and biophysics: imaging single (macro)molecules and measuring their dynamics on the femtosecond timescale to produce movies with atomic resolution.
Book Synopsis Introduction to Biophotonics by : Paras N. Prasad
Download or read book Introduction to Biophotonics written by Paras N. Prasad and published by John Wiley & Sons. This book was released on 2004-01-16 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt: Paras Prasad’s text provides a basic knowledge of a broad range of topics so that individuals in all disciplines can rapidly acquire the minimal necessary background for research and development in biophotonics. Introduction to Biophotonics serves as both a textbook for education and training as well as a reference book that aids research and development of those areas integrating light, photonics, and biological systems. Each chapter contains a topic introduction, a review of key data, and description of future directions for technical innovation. Introduction to Biophotonics covers the basic principles of Optics Optical spectroscopy Microscopy Each section also includes illustrated examples and review questions to test and advance the reader’s knowledge. Sections on biosensors and chemosensors, important tools for combating biological and chemical terrorism, will be of particular interest to professionals in toxicology and other environmental disciplines. Introduction to Biophotonics proves a valuable reference for graduate students and researchers in engineering, chemistry, and the life sciences.
