The Utilization of Microcolumn High-performance Liquid Chromatography in the Study of Proteins

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ISBN 13 :
Total Pages : 736 pages
Book Rating : 4.3/5 ( download)

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Book Synopsis The Utilization of Microcolumn High-performance Liquid Chromatography in the Study of Proteins by : Cheryl Lynn Flurer

Download or read book The Utilization of Microcolumn High-performance Liquid Chromatography in the Study of Proteins written by Cheryl Lynn Flurer and published by . This book was released on 1992 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt:

High-Performance Liquid Chromatography of Proteins and Peptides

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Author :
Publisher : Elsevier
ISBN 13 : 032315770X
Total Pages : 284 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis High-Performance Liquid Chromatography of Proteins and Peptides by : Milton Hearn

Download or read book High-Performance Liquid Chromatography of Proteins and Peptides written by Milton Hearn and published by Elsevier. This book was released on 2012-12-02 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.

High-Performance Liquid Chromatography of Peptides and Proteins

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Publisher : CRC Press
ISBN 13 : 1351440756
Total Pages : 960 pages
Book Rating : 4.3/5 (514 download)

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Book Synopsis High-Performance Liquid Chromatography of Peptides and Proteins by : Colin T. Mant

Download or read book High-Performance Liquid Chromatography of Peptides and Proteins written by Colin T. Mant and published by CRC Press. This book was released on 2017-11-22 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Microcolumn High-Performance Liquid Chromatography

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Publisher : Elsevier
ISBN 13 : 0080858317
Total Pages : 319 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Microcolumn High-Performance Liquid Chromatography by : P. Kucera

Download or read book Microcolumn High-Performance Liquid Chromatography written by P. Kucera and published by Elsevier. This book was released on 2000-04-01 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microcolumn High-Performance Liquid Chromatography

Hplc Of Biological Macro- Molecules, Revised And Expanded

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Publisher : CRC Press
ISBN 13 : 9780203911594
Total Pages : 806 pages
Book Rating : 4.9/5 (115 download)

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Book Synopsis Hplc Of Biological Macro- Molecules, Revised And Expanded by : Karen M. Gooding

Download or read book Hplc Of Biological Macro- Molecules, Revised And Expanded written by Karen M. Gooding and published by CRC Press. This book was released on 2002-01-08 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

HPLC of Peptides and Proteins

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Publisher : Springer Science & Business Media
ISBN 13 : 1592597424
Total Pages : 411 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis HPLC of Peptides and Proteins by : Marie-Isabel Aguilar

Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Microcolumn High-performance Liquid Chromatography

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Publisher :
ISBN 13 : 9780444416162
Total Pages : 0 pages
Book Rating : 4.4/5 (161 download)

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Book Synopsis Microcolumn High-performance Liquid Chromatography by : Paul Kucera

Download or read book Microcolumn High-performance Liquid Chromatography written by Paul Kucera and published by . This book was released on 1984 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

High-performance Liquid Chromatography of Cereal and Legume Proteins

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Publisher : American Association of Cereal Chemists
ISBN 13 : 9780913250822
Total Pages : 0 pages
Book Rating : 4.2/5 (58 download)

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Book Synopsis High-performance Liquid Chromatography of Cereal and Legume Proteins by : James E. Kruger

Download or read book High-performance Liquid Chromatography of Cereal and Legume Proteins written by James E. Kruger and published by American Association of Cereal Chemists. This book was released on 1994 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The evolution of cereal protein analysis by hplc; Practical considerations in the Rp-hplc analysis of cereal and legume proteins; Protein extraction and sample handling techniques; Data handling in Hplc and use of computers; Rp-hplc for varietal identification in cereals and legumes: wheat; rye and triticale; maize; barley; rice; oats; soybeans; sorghum; Rp-hplc for assessment of quality in cereals and legumes: breadmaking quality (wheat); Use of rp-hplc for a better understanding of the structure and functionality of wheat gluten proteins; Combined reversed-phase high-performance liquid chromatography (rp-hplc) and electrophoretic techniques in genetics and breeding of what storage proteins; Size-exclusion high-performance liquid chromatography for rapid examination of size differences of cereal proteins; Hplc ion-exchange chromatographic separations of cereal and legume proteins; Evaluation of hydrophobicity of wheat proteins and peptides by Hic and Rp-hplc.

Protein Liquid Chromatography

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Publisher : Elsevier
ISBN 13 :
Total Pages : 988 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Protein Liquid Chromatography by : M. Kastner

Download or read book Protein Liquid Chromatography written by M. Kastner and published by Elsevier. This book was released on 2000 with total page 988 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Microcolumn High-Performance Liquid Chromatography (Volume 28).

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Publisher :
ISBN 13 : 9780444416162
Total Pages : 302 pages
Book Rating : 4.4/5 (161 download)

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Book Synopsis Microcolumn High-Performance Liquid Chromatography (Volume 28). by : Kucera P.

Download or read book Microcolumn High-Performance Liquid Chromatography (Volume 28). written by Kucera P. and published by . This book was released on 1984 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Studies in the Separation of Proteins by High Performance Liquid Chromatography

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Publisher :
ISBN 13 :
Total Pages : 630 pages
Book Rating : 4.:/5 (24 download)

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Book Synopsis Studies in the Separation of Proteins by High Performance Liquid Chromatography by : Richard Eliot Shansky

Download or read book Studies in the Separation of Proteins by High Performance Liquid Chromatography written by Richard Eliot Shansky and published by . This book was released on 1988 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Application of High-performance Liquid Chromatography to the Study of Enzymes and Proteins

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (847 download)

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Book Synopsis Application of High-performance Liquid Chromatography to the Study of Enzymes and Proteins by : T. Nagatsu

Download or read book Application of High-performance Liquid Chromatography to the Study of Enzymes and Proteins written by T. Nagatsu and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

High-performance Liquid Chromatography of Proteins and Peptides

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (664 download)

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Book Synopsis High-performance Liquid Chromatography of Proteins and Peptides by :

Download or read book High-performance Liquid Chromatography of Proteins and Peptides written by and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

2-D Proteome Analysis Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592595847
Total Pages : 585 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis 2-D Proteome Analysis Protocols by : Andrew J. Link

Download or read book 2-D Proteome Analysis Protocols written by Andrew J. Link and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

High-Performance Liquid Chromatography of Peptides and Proteins

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Publisher : CRC Press
ISBN 13 : 1351440748
Total Pages : 887 pages
Book Rating : 4.3/5 (514 download)

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Book Synopsis High-Performance Liquid Chromatography of Peptides and Proteins by : Colin T. Mant

Download or read book High-Performance Liquid Chromatography of Peptides and Proteins written by Colin T. Mant and published by CRC Press. This book was released on 2017-11-22 with total page 887 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques

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Publisher : Elsevier
ISBN 13 : 0128245123
Total Pages : 956 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Carbohydrate Analysis by Modern Liquid Phase Separation Techniques by : Ziad El Rassi

Download or read book Carbohydrate Analysis by Modern Liquid Phase Separation Techniques written by Ziad El Rassi and published by Elsevier. This book was released on 2021-08-15 with total page 956 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. Covers the basic principles of modern liquid phase separation techniques, along with their applications Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress

Solid-Phase Microextraction

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Publisher : MDPI
ISBN 13 : 303928262X
Total Pages : 266 pages
Book Rating : 4.0/5 (392 download)

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Book Synopsis Solid-Phase Microextraction by : Constantinos K. Zacharis

Download or read book Solid-Phase Microextraction written by Constantinos K. Zacharis and published by MDPI. This book was released on 2020-02-07 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the most recent research activities and achievements regarding to the solid phase microextraction (SPME) technique. It is a powerful sample preparation tool that addresses the new challenges of analytical laboratories. Among others, its fundamental applications involved the sampling of volatile compounds from various matrixes. The demonstrated topics ranged from aroma characterization of various fruits, essential oils to the utilization of SPME for in-tube extraction and isolation of selected compounds from complex samples followed by state-of-the-art analytical techniques.