Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
The Role Of Protein Protein Interactions In The Phase Behavior Of Aqueous Protein Solutions
Download The Role Of Protein Protein Interactions In The Phase Behavior Of Aqueous Protein Solutions full books in PDF, epub, and Kindle. Read online The Role Of Protein Protein Interactions In The Phase Behavior Of Aqueous Protein Solutions ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis The Role of Protein-protein Interactions in the Phase Behavior of Aqueous Protein Solutions by : Camille O. Anderson
Download or read book The Role of Protein-protein Interactions in the Phase Behavior of Aqueous Protein Solutions written by Camille O. Anderson and published by . This book was released on 2001 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Role of Protein - Protein Interactions in the Phase Behaviour of Aqueous Protein Solutions by :
Download or read book The Role of Protein - Protein Interactions in the Phase Behaviour of Aqueous Protein Solutions written by and published by . This book was released on 2006 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Interactions and Phase Behavior in Aqueous Solutions by : Andre C. Dumetz
Download or read book Protein Interactions and Phase Behavior in Aqueous Solutions written by Andre C. Dumetz and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Thermodynamics of Protein Interactions and Phase Behavior in Aqueous Electrolyte Solution by : Christopher James Coen
Download or read book Molecular Thermodynamics of Protein Interactions and Phase Behavior in Aqueous Electrolyte Solution written by Christopher James Coen and published by . This book was released on 1995 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Thermodynamics of Protein Phase Behavior in Aqueous Electrolyte Solutions by : John Joseph Grigsby
Download or read book Molecular Thermodynamics of Protein Phase Behavior in Aqueous Electrolyte Solutions written by John Joseph Grigsby and published by . This book was released on 2001 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Effects of Salts on the Phase Behavior of Proteins and Protein Mixtures by : Yu-Chia Cheng
Download or read book Effects of Salts on the Phase Behavior of Proteins and Protein Mixtures written by Yu-Chia Cheng and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Separating proteins in aqueous solutions through fractional precipitation by addition of salts is one of the oldest protein separation methods, and it remains the only purification step for some industrial enzymes and an initial purification step for higher-value therapeutic proteins. However, despite its widespread application, the fundamental characteristics of protein precipitation by salt are still incompletely understood. The primary focus of this thesis is the experimental exploration of the phase behavior of single and binary protein systems during protein precipitation in concentrated ammonium sulfate and sodium chloride solutions, and the effect of solution conditions on protein interactions. Protein precipitation phase behavior of single protein systems was measured as a function of solution conditions for lysozyme and ovalbumin. X-ray powder diffraction (XRD) and optical microscopy were used to characterize the structure of the protein precipitates. These studies showed that crystallization after initial precipitation is feasible even at high ionic strengths if sufficient mixing time is allowed, and such behavior may be a more general phenomenon although different crystallization pathways may be found in some systems and under different conditions; for instance, different pathways are seen in ovalbumin-ammonium sulfate systems as a function of pH. A more important development is the interpretation that emerges of precipitation as a manifestation of a metastable liquid-liquid separation, with the precipitate being a kinetically trapped dense liquid phase. Furthermore, the diversity of possible dense phases (amorphous precipitate, crystal phases or gel) associated with liquid-liquid phase separation shows that understanding the effects of protein interactions of different kinds and at different ranges could have important consequences for the systematic control of protein phase behavior. The phase behavior in selective precipitation of lysozyme-ovalbumin binary mixtures was investigated at various conditions. The best combination of selectivity and recovery of lysozyme was achieved in pH 7 NaCl solutions. At an initial concentration of 30 mg/g H 2 O of lysozyme and 30 mg/g H 2 O of ovalbumin, more than 80% of the lysozyme was collected in precipitate form with 93% purity at ionic strengths above 3 m, while ovalbumin was collected in the supernatant at 80% purity. The purities of both proteins were therefore improved by at least 30% in one step. Moreover, crystallization following initial precipitation was observed in some binary precipitation experiments and indicates that binary precipitation, like single protein precipitation, is a metastable equilibrium state. The observations of selective precipitation behavior were correlated with values of the osmotic second virial coefficient, measured by cross-interaction chromatography (CIC). The results show that interaction measures such as protein self- and cross-interactions can be used as a predictor for general trends and/or inconsistencies in the separation behavior and for optimizing working conditions. Such a mechanistic relationship can obviate the need for extensive trial-and-error methods in order to optimize conditions for protein separations.
Book Synopsis Thermodynamic Modeling of Protein Interactions and Phase Behavior by : Leigh Quang
Download or read book Thermodynamic Modeling of Protein Interactions and Phase Behavior written by Leigh Quang and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein phase behavior encompasses the formation of dense phases, which include amorphous aggregates, gels, dense liquids, and crystals. The major solution variables that dictate the type of dense phase that is formed are pH, temperature, type of precipitant, precipitant concentration, and protein concentration. Because of the large parameter space and rich variety of phase transitions possible, protein phase behavior is a complex phenomenon. Fundamentally, macroscopic phase transitions are governed by the molecular interactions between proteins in solution. One promising way of quantifying protein-protein interactions and relating them to phase behavior is through the osmotic second virial coefficient B22, a dilute-solution property that characterizes two-particle interactions. The relationship of B22 to overall phase behavior of proteins is explored in this work. The goal of this thesis is to quantitatively relate protein-protein interactions to protein phase diagrams in order to develop predictive models of phase behavior under different solution conditions. A continuum-level approach is used initially to relate experimental B22 data and phase diagrams of proteins by appealing to existing thermodynamic models, with the expectation that a simple continuum model could provide a useful mechanistic framework for predicting protein phase behavior. The first approach attempted was to relate protein interactions and phase behavior within the Flory-Huggins theory of polymer solutions. The second approach utilized the model of Haas and Drenth, which is based on the free energy of mixing for hard spheres. Finally, phase equilibrium was predicted from virial coefficients using the osmotic virial equation. A qualitative relationship was found between B22 and phase behavior from these continuum models; however, quantitative agreement could not be obtained. The isotropic assumption shared among these models in addition to the orientationally-averaged nature of B22 suggests that the anisotropic character of protein interactions cannot be neglected, demonstrating the need for more detailed molecular-level models. The role of anisotropy in protein interactions was explored through analysis of "patch-antipatch" pairs in the computation of B22 in atomistic detail. Patch-antipatch pairs represent highly attractive orientations resulting from geometric complementarity between protein surfaces. Previous work used simple Monte Carlo integration for the calculation of B22 from atomistic models of proteins. However, the presence of patch-antipatch pairs led to significant numerical concerns. These concerns warranted a reexamination of the numerical methods for computing B22. A hybrid Monte Carlo/patch integration approach is utilized to calculate B22 for lysozyme and chymosin B. This method involves a combination of numerical integration techniques in an attempt to obtain better convergence in predicting B22. The overall B22 for the proteins studied was separated into three components: contributions from the excluded volume, from the patch-antipatch pairs, and from background configurations. The excluded volume component was found to be adequately determined using simple Monte Carlo integration. The contributions from individual patch-antipatch pairs were accounted for by carefully integrating the subregions of the configuration space occupied by these pairs using a globally adaptive integration routine. The background component to B22 was also calculated by simple Monte Carlo integration in which the regions of the configuration space occupied by the patch-antipatch pairs were excluded. The calculations performed that account for the full protein structure emphasize the importance of several features of protein interactions. First, the difference in the interaction behavior of the two proteins studied was found to be largely attributed to the charge anisotropy of patch-antipatch pairs. However, the relation of the results to experimental data is limited by the omission of accounting for the specific hydration of proteins. Hydration effects are known to affect, and usually attenuate, patch-antipatch configurations, and therefore would be expected to significantly impact the accurate prediction of B22. Classical colloidal as well as atomistic models that omit these important features are inadequate in providing a quantitative representation of protein interactions for a wide range of solution conditions.
Book Synopsis Protein-Solvent Interactions by : Roger Gregory
Download or read book Protein-Solvent Interactions written by Roger Gregory and published by CRC Press. This book was released on 1995-01-04 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work covers advances in the interactions of proteins with their solvent environment and provides fundamental physical information useful for the application of proteins in biotechnology and industrial processes. It discusses in detail structure, dynamic and thermodynamic aspects of protein hydration, as well as proteins in aqueous and organic solvents as they relate to protein function, stability and folding.
Download or read book Protein Interactions written by and published by VCH Publishers. This book was released on 1992 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: A knowledge of protein interaction is indispensable to successful research in biochemistry. However, the scope of relevant topics is astoundingly broad, ranging from molecular to colloidal chemistry. It is often difficult to find sufficient up-to-date information in any particular subarea. This book concentrates on two fascinating subjects in the field: * stabilization of micelles in solution * coagulation processes involved in cheese formation. 19 contributions by leading laboratories worldwide describe the most recent developments in detail. Written from both theoretical and applied points of view, the publication is a stimulating source of information, above all, for protein researchers in food chemistry. Special feature: in-depth presentation of a novel model of the casein micelle in milk and the role of water in protein interactions.
Book Synopsis Biothermodynamics by : Urs von Stockar
Download or read book Biothermodynamics written by Urs von Stockar and published by CRC Press. This book was released on 2013-05-30 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of the rapidly growing field of biothermodynamics, showing how thermodynamics can best be applied to applications and processes in biochemical engineering. It describes the rigorous application of thermodynamics in biochemical engineering to rationalize bioprocess development and obviate a substantial fraction of this need for tedious experimental work. As such, this book will appeal to a diverse group of readers, ranging from students and professors in biochemical engineering, to scientists and engineers, for whom it will be a valuable reference.
Book Synopsis An Investigation of Protein Interactions and the Relationship to Phase Behavior by : Darren Fredrick Rosenbaum
Download or read book An Investigation of Protein Interactions and the Relationship to Phase Behavior written by Darren Fredrick Rosenbaum and published by . This book was released on 1998 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Self-Assembly by : Jennifer J. McManus
Download or read book Protein Self-Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Book Synopsis Proceedings of the 4th International Conference on Separations Science and Technology by : Zhangfa Tong
Download or read book Proceedings of the 4th International Conference on Separations Science and Technology written by Zhangfa Tong and published by World Scientific. This book was released on 2004 with total page 1062 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest achievements of separation science and technology. It highlights the application of separation with regard to problems of current interest, such as the protection of the environment and the development of emerging technology, including chemical engineering, biotechnology, renewable energy sources and recycling of materials.
Book Synopsis Molecular Thermodynamics of Proteins in Aqueous Solutions of Concentrated Electrolyte by : Robin Andrew Curtis
Download or read book Molecular Thermodynamics of Proteins in Aqueous Solutions of Concentrated Electrolyte written by Robin Andrew Curtis and published by . This book was released on 2000 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Interactions in Solution by : Wei Liu
Download or read book Protein Interactions in Solution written by Wei Liu and published by . This book was released on 2004 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structure and Phase Behavior of Protein Solutions by :
Download or read book Structure and Phase Behavior of Protein Solutions written by and published by . This book was released on 2011 with total page 27 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 1006 pages. Available in PDF, EPUB and Kindle. Book excerpt:
