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The Resolution And Contrast Of Confocal And Two Photon Laser Scanning Fluorescence Microscopy In Turbid Media
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Book Synopsis The Resolution and Contrast of Confocal and Two-photon Laser Scanning Fluorescence Microscopy in Turbid Media by : Yuin-Jen D. Hsu
Download or read book The Resolution and Contrast of Confocal and Two-photon Laser Scanning Fluorescence Microscopy in Turbid Media written by Yuin-Jen D. Hsu and published by . This book was released on 1996 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Confocal and Two-Photon Microscopy by : Alberto Diaspro
Download or read book Confocal and Two-Photon Microscopy written by Alberto Diaspro and published by John Wiley & Sons. This book was released on 2001-11-22 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal and Two-Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy. Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring: * In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications * Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules * Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references * Companion FTP site with full-color photographs * The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.
Book Synopsis Confocal Laser Scanning Microscopy by : C. Sheppard
Download or read book Confocal Laser Scanning Microscopy written by C. Sheppard and published by BIOS Scientific Publishers. This book was released on 1997-09-26 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the principles of the technique and illustrates applications in physical and biomedical sciences. Covers Image formation in confocal microscopy, Performance of the confocal microscope, Biological and industrial applications. Paper. DLC: Confocal microscopy.
Book Synopsis Microscopic Imaging Through Turbid Media by : Min Gu
Download or read book Microscopic Imaging Through Turbid Media written by Min Gu and published by Springer. This book was released on 2015-04-08 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a systematic introduction to the principles of microscopic imaging through tissue-like turbid media in terms of Monte-Carlo simulation. It describes various gating mechanisms based on the physical differences between the un scattered and scattered photons and method for microscopic image reconstruction, using the concept of the effective point spread function. Imaging an object embedded in a turbid medium is a challenging problem in physics as well as in bio photonics. A turbid medium surrounding an object under inspection causes multiple scattering, which degrades the contrast, resolution and signal-to-noise ratio. Biological tissues are typically turbid media. Microscopic imaging through a tissue-like turbid medium can provide higher resolution than transillumination imaging in which no objective is used. This book serves as a valuable reference for engineers and scientists working on microscopy of tissue turbid media.
Book Synopsis Confocal Microscopy and Multiphoton Excitation Microscopy by : Barry R. Masters
Download or read book Confocal Microscopy and Multiphoton Excitation Microscopy written by Barry R. Masters and published by SPIE Press. This book was released on 2006 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.
Book Synopsis Science of Microscopy by : P.W. Hawkes
Download or read book Science of Microscopy written by P.W. Hawkes and published by Springer Science & Business Media. This book was released on 2008-08-29 with total page 1336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully corrected second impression of the classic 2006 text on microscopy runs to more than 1,000 pages and covers up-to-the-minute developments in the field. The two-volume work brings together a slew of experts who present comprehensive reviews of all the latest instruments and new versions of the older ones, as well as their associated operational techniques. The chapters draw attention to their principal areas of application. A huge range of subjects are benefiting from these new tools, including semiconductor physics, medicine, molecular biology, the nanoworld in general, magnetism, and ferroelectricity. This fascinating book will be an indispensable guide for a wide range of scientists in university laboratories as well as engineers and scientists in industrial R&D departments.
Book Synopsis Real-time Two-photon Excited Fluorescence Laser-scanned Microscopy by : Jeffrey Barton Guild
Download or read book Real-time Two-photon Excited Fluorescence Laser-scanned Microscopy written by Jeffrey Barton Guild and published by . This book was released on 1997 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck
Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-06-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
Book Synopsis Handbook of Biological Confocal Microscopy by : James Pawley
Download or read book Handbook of Biological Confocal Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2006-06-02 with total page 1018 pages. Available in PDF, EPUB and Kindle. Book excerpt: Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
Book Synopsis Handbook of Biomedical Fluorescence by : Mary-Ann Mycek
Download or read book Handbook of Biomedical Fluorescence written by Mary-Ann Mycek and published by CRC Press. This book was released on 2019-07-17 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: Melding basic and clinical science, this reference provides a comprehensive overview of the roles that biophysics, photochemistry, and computational modeling play in the biomedical applications of fluorescence spectroscopy and imaging. Penned by pioneering researchers, the Handbook of Biomedical Fluorescence discusses fundamental aspects of fluorescence generation in organic molecules within tissue, theoretical and experimental views of how light propagation in tissue can be used to interpret fluorescence signals, endogenous and exogenous fluorescence agents in medical or basic research studies, and radiation transport, diffusion theory, and the Monte Carlo method.
Book Synopsis The Design, Construction, and Operation of a Two-photon Laser Scanning Confocal Microscope by : Benjamin Alexander
Download or read book The Design, Construction, and Operation of a Two-photon Laser Scanning Confocal Microscope written by Benjamin Alexander and published by . This book was released on 2011 with total page 33 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal microscopes have become valuable tools in many fields of study. This work is intended to serve as a primer for basic confocal microscopy theory as well as a description of the design and construction of a homebuilt system in the Elson Lab at the Washington University School of Medicine. A description of the different designs of confocal microscopes is provided, and the capabilities, benefits, and detriments of each is described. The thought process behind each design decision is explained leading to the final system design. A more in depth description of the final design is provided in Chapters Two and Three, but, in short, the final design is a non-descanned, laser scanning, two-photon confocal microscope. The system is designed to integrate as seamlessly as possible with the existing system and to allow for all existing functionality to remain. When completed, the system will be capable of one- and two-photon fluorescence correlation ii spectroscopy (FCS); low-speed, one-photon confocal imaging using a piezo-stage; and high-speed, two-photon confocal imaging using the laser scanning system. Finally, future directions as well as the limitations of the system are also described. All resources necessary to continue work on the system as well as those necessary to use it are provided. This includes: System diagrams, Optical layout plots, Component data sheets, LabVIEW program. These resources are intended to make using, modifying, and improving the system much simpler.
Book Synopsis Lasers and Current Optical Techniques in Biology by : Giuseppe Palumbo
Download or read book Lasers and Current Optical Techniques in Biology written by Giuseppe Palumbo and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of innovative light sources, fibre laser sources and light emitting diodes, is opening unexpected perspectives into optical techniques and is promising new exciting applications in the field of biomedicine. Lasers and Current Optical Techniques in Biology aims to provide an overview of light sources, together with an extensive and authoritative description of the optical techniques in bio-medicine. This book is designed to give biomedical researchers a strong feel for the capability of physical approaches, promote new interdisciplinary interests and persuade more practitioners to take advantage of optical techniques. Current developments in a variety of optical techniques, including Near-Infra Red Spectroscopy, and traditional and advanced fluorescence techniques are covered, ranging from those that are becoming common practice to those that need much more experimentation before they can be accepted as real breakthroughs. Further topics include optical coherence tomography and its variations, polarised light imaging and, principle laser and lamp sources- a usually fragmentary topic, often dispersed among specialist publications. The wide range of topics covered make Lasers and Current Optical Techniques in Biology of interest to a diverse range of scientific communities.
Book Synopsis Introduction to Confocal Fluorescence Microscopy by : Michiel Muller
Download or read book Introduction to Confocal Fluorescence Microscopy written by Michiel Muller and published by SPIE Press. This book was released on 2006 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.
Book Synopsis Second Harmonic Generation Imaging by : Francesco S. Pavone
Download or read book Second Harmonic Generation Imaging written by Francesco S. Pavone and published by Taylor & Francis. This book was released on 2016-04-19 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
Book Synopsis High Resolution Imaging in Microscopy and Ophthalmology by : Josef F. Bille
Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Book Synopsis Multiphoton Microscopy and Fluorescence Lifetime Imaging by : Karsten König
Download or read book Multiphoton Microscopy and Fluorescence Lifetime Imaging written by Karsten König and published by Walter de Gruyter GmbH & Co KG. This book was released on 2018-01-22 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography
Book Synopsis Confocal, Multiphoton, and Nonlinear Microscopic Imaging by :
Download or read book Confocal, Multiphoton, and Nonlinear Microscopic Imaging written by and published by . This book was released on 2005 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
