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The Protoplast
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Download or read book Protoplast Technology written by Kan Wang and published by Humana. This book was released on 2022-03-09 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.
Download or read book Protoplast Fusion written by Y. Y. Gleba and published by Springer. This book was released on 2011-12-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.
Download or read book Fungal Protoplasts written by J. Peberdy and published by CRC Press. This book was released on 1985-02-20 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.
Book Synopsis Progress in Plant Protoplast Research by : K.J. Puite
Download or read book Progress in Plant Protoplast Research written by K.J. Puite and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987
Book Synopsis Fungal Protoplast by : D Lalithakumari
Download or read book Fungal Protoplast written by D Lalithakumari and published by CRC Press. This book was released on 2019-04-24 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: Better validation of indigenous domestic animal genetic resources is becoming more important with regard to the potential of livestock for poverty alleviation and income generation. To improve indigenous breeds for sustainable income and employment generation, the methods to be employed are the same as developed in systematic breeding programs, be it for cross-breeding or selective breeding within a specific breed. This book systematically introduces the reader to the breed improvement theory and illustrates the theory with practical examples and case studies. The book is addressed to animal science teachers, to undergraduate and postgraduate students as well as to decision makers in the state and central livestock departments.
Book Synopsis Plant Cell, Tissue and Organ Culture by : Oluf Gamborg
Download or read book Plant Cell, Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Book Synopsis The protoplast, a series of papers [by E.C.C. Baillie]. by : E C C. Baillie
Download or read book The protoplast, a series of papers [by E.C.C. Baillie]. written by E C C. Baillie and published by . This book was released on 1853 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plant Tissue Culture Manual by : K. Lindsey
Download or read book Plant Tissue Culture Manual written by K. Lindsey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.
Book Synopsis Esau's Plant Anatomy by : Ray F. Evert
Download or read book Esau's Plant Anatomy written by Ray F. Evert and published by John Wiley & Sons. This book was released on 2006-08-28 with total page 623 pages. Available in PDF, EPUB and Kindle. Book excerpt: This revision of the now classic Plant Anatomy offers a completely updated review of the structure, function, and development of meristems, cells, and tissues of the plant body. The text follows a logical structure-based organization. Beginning with a general overview, chapters then cover the protoplast, cell wall, and meristems, through to phloem, periderm, and secretory structures. "There are few more iconic texts in botany than Esau’s Plant Anatomy... this 3rd edition is a very worthy successor to previous editions..." ANNALS OF BOTANY, June 2007
Book Synopsis An Introduction to Plant Structure and Development by : Charles B. Beck
Download or read book An Introduction to Plant Structure and Development written by Charles B. Beck and published by Cambridge University Press. This book was released on 2010-04-22 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: A plant anatomy textbook unlike any other on the market today. Carol A. Peterson described the first edition as 'the best book on the subject of plant anatomy since the texts of Esau'. Traditional plant anatomy texts include primarily descriptive aspects of structure, this book not only provides a comprehensive coverage of plant structure, but also introduces aspects of the mechanisms of development, especially the genetic and hormonal controls, and the roles of plasmodesmata and the cytoskeleton. The evolution of plant structure and the relationship between structure and function are also discussed throughout. Includes extensive bibliographies at the end of each chapter. It provides students with an introduction to many of the exciting, contemporary areas at the forefront of research in the development of plant structure and prepares them for future roles in teaching and research in plant anatomy.
Book Synopsis The Molecular Biology of Plant Cells by : H. Smith
Download or read book The Molecular Biology of Plant Cells written by H. Smith and published by Univ of California Press. This book was released on 1977-01-01 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
Book Synopsis Plant Protoplasts and Genetic Engineering by : Y. P. S. Bajaj
Download or read book Plant Protoplasts and Genetic Engineering written by Y. P. S. Bajaj and published by Springer. This book was released on 1989 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis On the Relationship of the Nuclear Membrane to the Protoplast ... by : Anstruther Abercrombie Lawson
Download or read book On the Relationship of the Nuclear Membrane to the Protoplast ... written by Anstruther Abercrombie Lawson and published by . This book was released on 1903 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Experimental Manipulation of Gene Expression by : Masayori Inouye
Download or read book Experimental Manipulation of Gene Expression written by Masayori Inouye and published by Academic Press. This book was released on 2014-06-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
Book Synopsis Plant Cell Culture by : Michael R. Davey
Download or read book Plant Cell Culture written by Michael R. Davey and published by John Wiley & Sons. This book was released on 2010-05-20 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
Author :Víctor M. Loyola-Vargas Publisher :Springer Science & Business Media ISBN 13 :1592599591 Total Pages :389 pages Book Rating :4.5/5 (925 download)
Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas
Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
Book Synopsis Plant Cell and Tissue Culture by : J. Reinert
Download or read book Plant Cell and Tissue Culture written by J. Reinert and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.