The Polymerase Chain Reaction Pcr For Human Viral Diagnosis

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The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis

Author : Jonathan P. Clewley
Publisher : CRC Press
ISBN 13 : 9780849348334
Total Pages : 244 pages
Book Rating : 4.3/5 (483 download)

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Book Synopsis The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis by : Jonathan P. Clewley

Download or read book The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis written by Jonathan P. Clewley and published by CRC Press. This book was released on 1994-10-27 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.

Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Author : Yechiel Becker
Publisher : Springer Science & Business Media
ISBN 13 : 3642847668
Total Pages : 428 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Diagnosis of Human Viruses by Polymerase Chain Reaction Technology by : Yechiel Becker

Download or read book Diagnosis of Human Viruses by Polymerase Chain Reaction Technology written by Yechiel Becker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basis for the effective treatment and cure of a patient is the rapid diagnosis of the disease and its causative agent, which is based on the analysis of the clinical symptoms coupled with laboratory tests. Although rapid advance ments have been made in the laboratory diagnosis of virus diseases, the neces sary isolation of the causative virus from the clinical specimens is a relatively long procedure. Viruses which integrate into the cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses which exist in the clinical material in low concentrations are even more formidable to identify. Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (1985) led to a revolution in virus diagnosis. The peR technique was rapidly applied to the diagnosis of viruses in clinical material. Volume 1 of Frontiers of Virology provides new information on the advan tages of the use of the peR for the diagnosis of many human disease-causing viruses, as well as on some problems with its use.

Molecular Methods for Virus Detection

Author : Danny L. Wiedbrauk
Publisher : Elsevier
ISBN 13 : 0080536891
Total Pages : 405 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Molecular Methods for Virus Detection by : Danny L. Wiedbrauk

Download or read book Molecular Methods for Virus Detection written by Danny L. Wiedbrauk and published by Elsevier. This book was released on 1995-02-08 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables

PCR: Protocols for Diagnosis of Human and Animal Virus Diseases

Author : Yechiel Becker
Publisher : Springer
ISBN 13 :
Total Pages : 622 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis PCR: Protocols for Diagnosis of Human and Animal Virus Diseases by : Yechiel Becker

Download or read book PCR: Protocols for Diagnosis of Human and Animal Virus Diseases written by Yechiel Becker and published by Springer. This book was released on 1995-09-26 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt: Today the diagnosis of human and animal pathogenic viruses would be unthinkable without the polymerase chain reaction (PCR). The short-styled protocols in this volume elucidate this important technique and its application in the laboratory, including a discussion of recent advances in PCR technology. The enclosed diskette enables the user to work with interesting sequences on his own computer.

PCR Topics

Author : Arndt Rolfs
Publisher : Springer Science & Business Media
ISBN 13 : 3642759246
Total Pages : 258 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis PCR Topics by : Arndt Rolfs

Download or read book PCR Topics written by Arndt Rolfs and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

PCR Technology

Author : Henry Erlich
Publisher : Springer
ISBN 13 : 1349202355
Total Pages : 246 pages
Book Rating : 4.3/5 (492 download)

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Book Synopsis PCR Technology by : Henry Erlich

Download or read book PCR Technology written by Henry Erlich and published by Springer. This book was released on 2015-12-31 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

WHO Expert Consultation on Rabies

Author : World Health Organization
Publisher : World Health Organization
ISBN 13 : 9241209313
Total Pages : 121 pages
Book Rating : 4.2/5 (412 download)

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Book Synopsis WHO Expert Consultation on Rabies by : World Health Organization

Download or read book WHO Expert Consultation on Rabies written by World Health Organization and published by World Health Organization. This book was released on 2005-12-31 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: More than 99% of all human rabies deaths occur in the developing world and although effective and economical control measures are available the disease has not been brought under control throughout most of the affected countries. Given that a major factor in the low level of commitment to rabies control is a lack of accurate data on the true public health impact of the disease this report of a WHO Expert Consultation begins by providing new data on the estimated burden of the disease and its distribution in the world. It also reviews recent progress in the classification of rabies viruses rabies pathogenesis and diagnosis rabies pre- and post-exposure prophylaxis the management of rabies patients and canine as well as wildlife rabies prevention and control.

Lennette's Laboratory Diagnosis of Viral Infections

Author : Keith R Jerome
Publisher : CRC Press
ISBN 13 : 1420084968
Total Pages : 512 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Lennette's Laboratory Diagnosis of Viral Infections by : Keith R Jerome

Download or read book Lennette's Laboratory Diagnosis of Viral Infections written by Keith R Jerome and published by CRC Press. This book was released on 2016-04-19 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written from the perspective of the diagnostician, this bestselling book is the definitive text on the laboratory diagnosis of human viral diseases. It contains a wealth of illustrations, tables, and algorithms to enhance your understanding of this ever-evolving field. The book is a ready reference for virologists, microbiologists, epidemiologists, laboratorians, and infections disease specialists, and students.

Polymerase Chain Reaction

Author : Shehnam Shafique
Publisher : Lulu.com
ISBN 13 : 1105728935
Total Pages : 84 pages
Book Rating : 4.1/5 (57 download)

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Book Synopsis Polymerase Chain Reaction by : Shehnam Shafique

Download or read book Polymerase Chain Reaction written by Shehnam Shafique and published by Lulu.com. This book was released on 2012-05-05 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: This PCR handbook establishes an easy approach for the students going through their studies in Molecular Diagnostics. It introduces timely topics such as Clinical Applications of PCR, Real time PCR, PCR Optimization, Application of PCR, PCR Array System Performance, Designing the primers, PCR in diagnosis of diseases and different PCR Techniques and their procedures.

The Polymerase Chain Reaction

Author : Kary B. Mullis
Publisher : Springer Science & Business Media
ISBN 13 : 1461202574
Total Pages : 464 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis The Polymerase Chain Reaction by : Kary B. Mullis

Download or read book The Polymerase Chain Reaction written by Kary B. Mullis and published by Springer Science & Business Media. This book was released on 2012-02-02 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

The Polymerase Chain Reaction

Author : Kary B. Mullis
Publisher : Springer Science & Business Media
ISBN 13 : 9780817636074
Total Pages : 0 pages
Book Rating : 4.6/5 (36 download)

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Book Synopsis The Polymerase Chain Reaction by : Kary B. Mullis

Download or read book The Polymerase Chain Reaction written by Kary B. Mullis and published by Springer Science & Business Media. This book was released on 1994 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: One: Methodology.- I. Basic Methodology.- 1. Manipulation of DNA by PCR.- 2. Cloning PCR Products.- 3. Optimization of Multiplex PCRs.- 4. Preparation of Nucleic Acids for Archival Material.- 5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ.- II. Quantitation.- 6. Quantitative PCR: An Overview.- 7. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control.- 8. RT-PCR and mRNA Quantitation.- 9. Analysis of Human T-Cell Repertoires by PCR.- III. Nonisotopic Detection.- 10. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview.- 11. Fluorescent Detection Methods for PCR Analysis.- 12. Enzyme-Labeled Oligonucleotides.- 13. Application of the Hybridization Protection Assay (HPA) to PCR.- IV. Instrumentation.- 14. PCR Instrumentation: Where Do We Stand?.- 15. Rapid Cycle DNA Amplification.- 16. Automating the PCR Process.- V. Sequencing.- 17. PCR and DNA Sequencing.- 18. Phage Promoter-Based Methods for Sequencing and Screening for Mutations.- 19. Capture PCR: An Efficient Method for Walking Along Chromosomal DNA and cDNA.- Two: Applications.- I. General Applications.- 20. In Vitro Evolution of Functional Nucleic Acids: High-Affinity RNA Ligands of the HIV-1 rev Protein.- 21. The Application of PCR to Forensic Science.- 22. Recreating the Past by PCR.- 23. Nonbiological Applications.- II. Genetic Analysis.- 24. RT-PCR and Gene Expression.- 25. Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs.- 26. Genetics, Plants, and the Polymerase Chain Reaction.- III. Assessment of Therapy Effectiveness.- 27. PCR Assessment of the Efficacy of Therapy in Philadelphia Chromosome-Positive Leukemias.- 28. The Detection of Minimal Residual Disease (MRD) in Acute Lymphoblastic Leukemia Using Clone-Specific Probes Directed against V(D)J Junctional Sequences.- 29. Assessment of Therapy Effectiveness: Infectious Disease.- 30. Gene Therapy.- IV. Diagnostics.- 31. PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes.- 32. Clinical Applications of the Polymerase Chain Reaction.- 33. Infectious Diseases.- Three: PCR and the World of Business.- 34. PCR in the Marketplace.- 35. PCR and Scientific Invention: The Trial of DuPont vs. Cetus.

Global Infectious Disease Surveillance and Detection

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309111145
Total Pages : 284 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Global Infectious Disease Surveillance and Detection by : Institute of Medicine

Download or read book Global Infectious Disease Surveillance and Detection written by Institute of Medicine and published by National Academies Press. This book was released on 2007-11-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.

PCR in Bioanalysis

Author : Stephen J Meltzer
Publisher : Springer Science & Business Media
ISBN 13 : 1592595758
Total Pages : 286 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis PCR in Bioanalysis by : Stephen J Meltzer

Download or read book PCR in Bioanalysis written by Stephen J Meltzer and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

Rapid PCR-based Monitoring of Infectious Enteroviruses in Drinking Water

Author : Kelly A. Reynolds
Publisher : American Water Works Association
ISBN 13 : 1583211012
Total Pages : 90 pages
Book Rating : 4.5/5 (832 download)

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Book Synopsis Rapid PCR-based Monitoring of Infectious Enteroviruses in Drinking Water by : Kelly A. Reynolds

Download or read book Rapid PCR-based Monitoring of Infectious Enteroviruses in Drinking Water written by Kelly A. Reynolds and published by American Water Works Association. This book was released on 2001 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the results of a study which used a combination integrated cell culture/PCR (ICC/PCR) technique for detecting human enteric viruses in raw and finished drinking water. The technique allows for definitive detection of infectious enteroviruses in days compared to weeks with cell culture alone. In addition, ICC/PCR eliminates the traditional flaw of PCR analysis alone, which cannot distinguish between infectious virus versus noninfectious virus. The text describes the materials and methods used, reports the results in testing for poliovirus and Hepatitis A, and discusses recommendations for water utilities. The volume is not indexed. c. Book News Inc.

Molecular Virology

Author : Susanne Modrow
Publisher : Springer
ISBN 13 : 9783642207174
Total Pages : 0 pages
Book Rating : 4.2/5 (71 download)

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Book Synopsis Molecular Virology by : Susanne Modrow

Download or read book Molecular Virology written by Susanne Modrow and published by Springer. This book was released on 2013-09-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.

Polymerase Chain Reaction for Biomedical Applications

Author : Ali Samadikuchaksaraei
Publisher : BoD – Books on Demand
ISBN 13 : 9535127950
Total Pages : 186 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Polymerase Chain Reaction for Biomedical Applications by : Ali Samadikuchaksaraei

Download or read book Polymerase Chain Reaction for Biomedical Applications written by Ali Samadikuchaksaraei and published by BoD – Books on Demand. This book was released on 2016-12-14 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.

Molecular Virology of Human Pathogenic Viruses

Author : Wang-Shick Ryu
Publisher : Academic Press
ISBN 13 : 0128009993
Total Pages : 440 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Molecular Virology of Human Pathogenic Viruses by : Wang-Shick Ryu

Download or read book Molecular Virology of Human Pathogenic Viruses written by Wang-Shick Ryu and published by Academic Press. This book was released on 2016-03-30 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author’s more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. Presents viruses within their family structure Contains recommended journal articles with perspectives to put primary literature in context Includes integrated recommended reading references within each chapter Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor’s manual, study guide, and test bank