The Baculoviruses

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Publisher : Springer Science & Business Media
ISBN 13 : 1489918345
Total Pages : 459 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis The Baculoviruses by : Lois K. Miller

Download or read book The Baculoviruses written by Lois K. Miller and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.

Baculovirus and Insect Cell Expression Protocols

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Publisher : Methods in Molecular Biology
ISBN 13 : 9781493979783
Total Pages : 522 pages
Book Rating : 4.9/5 (797 download)

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Book Synopsis Baculovirus and Insect Cell Expression Protocols by : David W. Murhammer

Download or read book Baculovirus and Insect Cell Expression Protocols written by David W. Murhammer and published by Methods in Molecular Biology. This book was released on 2018-04-15 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

The Molecular Biology of Baculoviruses

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Publisher :
ISBN 13 : 9783642715907
Total Pages : 182 pages
Book Rating : 4.7/5 (159 download)

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Book Synopsis The Molecular Biology of Baculoviruses by : Walter Doerfler

Download or read book The Molecular Biology of Baculoviruses written by Walter Doerfler and published by . This book was released on 2014-08-15 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:

BIOLOGY OF BACULOVIRUSES

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Publisher : Springer
ISBN 13 :
Total Pages : 296 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis BIOLOGY OF BACULOVIRUSES by : Robert R. Granados

Download or read book BIOLOGY OF BACULOVIRUSES written by Robert R. Granados and published by Springer. This book was released on 1986 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: V.1 - Biological properties and molecular biology: Historical perspectives; Taxonomy and identification of baculoviruses; Ultrastructure of baculoviruses; In vivo infection and replication of baculoviruses; In vitro replication of baculoviruses; Baculovirus structural proteins and protein synthesis; Current developments in baculovirus serology; Persistent baculovirus infections; Specificity and safety of baculoviruses; Evolution of occluded baculoviruses; The genetics of baculoviruses; Organization and expression of the baculovirus genome; v.2 - Practical application for insect control: In vivo and in vitro bioassay methods for baculoviruses; In vivo production of baculoviruses; Cell culture methods for large-scale propagation of baculoviruses; Ecology and ens, chemical pesticides, and parasitoids; Formulation and application of baculoviruses; Use of baculoviruses in pest management programs; Registration of baculoviruses as pesticides; Practical factors influencing the utilization of baculoviruses as pesticides; Insect resistance to baculoviruses.

Baculovirus Expression Systems and Biopesticides

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Author :
Publisher : Wiley-Liss
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Baculovirus Expression Systems and Biopesticides by : Michael L. Shuler

Download or read book Baculovirus Expression Systems and Biopesticides written by Michael L. Shuler and published by Wiley-Liss. This book was released on 1995 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developing effective baculovirus-insect culture systems. Insect cell culture methods and their use in virus research. Comparison of mammalian and insect cell cultures. protein production and processing from baculovirus. Development and testing of genetically improved baculovirus insecticides. Fundamentals of baculovirus-insect cell attachment and infection. Development and evaluation of host insect cells. Bioreactor design and scale-up issues. The effect of hydrodynamic forces on insect cells. Commercial application of insect cell culture. Baculovirus-mediated production of proteins in insect cells. Potential application of insect cell-based expression systems in the bio/pharmaceutical industry.

Insect Cell Culture Engineering

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Publisher : CRC Press
ISBN 13 : 1000105660
Total Pages : 260 pages
Book Rating : 4.0/5 (1 download)

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Book Synopsis Insect Cell Culture Engineering by : Mattheus F. A. Goosen

Download or read book Insect Cell Culture Engineering written by Mattheus F. A. Goosen and published by CRC Press. This book was released on 2020-07-24 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (748 download)

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Book Synopsis Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology by : David W. Murhammer

Download or read book Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology written by David W. Murhammer and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Baculovirus Expression Protocols" is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the "Methods in Molecular Biology" series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of "Baculovirus Expression Protocols" provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

Insect Viruses

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Publisher : Academic Press
ISBN 13 : 0120398680
Total Pages : 513 pages
Book Rating : 4.1/5 (23 download)

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Book Synopsis Insect Viruses by : Byrony C Bonning

Download or read book Insect Viruses written by Byrony C Bonning and published by Academic Press. This book was released on 2006-09-22 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Baculovirus Expression Vectors

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Publisher :
ISBN 13 : 9780195091311
Total Pages : 366 pages
Book Rating : 4.0/5 (913 download)

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Book Synopsis Baculovirus Expression Vectors by : David R. O'Reilly

Download or read book Baculovirus Expression Vectors written by David R. O'Reilly and published by . This book was released on 1994 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.

Baculovirus Expression Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 159259526X
Total Pages : 412 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Baculovirus Expression Protocols by : Christopher D. Richardson

Download or read book Baculovirus Expression Protocols written by Christopher D. Richardson and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.

Current Issues in Molecular Virology

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Publisher : BoD – Books on Demand
ISBN 13 : 9535112074
Total Pages : 297 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Current Issues in Molecular Virology by : Victor Romanowski

Download or read book Current Issues in Molecular Virology written by Victor Romanowski and published by BoD – Books on Demand. This book was released on 2013-11-20 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a collection of chapters dealing with examples of RNA and DNA viruses, and issues such as how these gene packages have learnt to take advantage of their hosts, molecular recognition events that hosts may use to counterattack the viruses, and how researchers have developed strategies to use viruses or their parts as tools for different purposes.

Insect Pathology

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Publisher : Academic Press
ISBN 13 : 0080926258
Total Pages : 681 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Insect Pathology by : Yoshinori Tanada

Download or read book Insect Pathology written by Yoshinori Tanada and published by Academic Press. This book was released on 2012-12-02 with total page 681 pages. Available in PDF, EPUB and Kindle. Book excerpt: Insect Pathology is designed for a broad spectrum of readers. Is should be useful to students, lecturers, and researchers requiring information about the principles in insect pathology and the biology of pathogens. It should serve as a resource for specialists to learn about other insect pathogen systems, for generalists to become aware of advances in insect pathology, and for scientists and students, beginning or otherwise, interested in learning about insect pathology. This book was originally intended to update the 1949 test by E. A. Steinhaus entitled Principles of Insect Pathology. The purpose for this book was twofold: To serve (1) as a text for an insect pathology and/or biological control class and (2) as a comprehensive reference source. Because this book summarizes much of the available information, its usefulness as a textbook for an insect pathology class is apparent. Although the literature citations are extensive, they are far from complete. The literature in insect pathology is voluminous and for the past decade has been expanding at an almost exponential rate. A complete review of the literature is beyond the scope of the book, and an omission of a reference does not preclude its importance. Our citations, however, should serve as a good starting point for those who wish to obtain further information. We have attempted to cover equally all subdisciplines, but shortcomings are unavoidable. For these, we take full responsibility.

Role of Apoptosis in Infection

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Publisher : Springer Science & Business Media
ISBN 13 : 3540273204
Total Pages : 300 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Role of Apoptosis in Infection by : Diane E. Griffin

Download or read book Role of Apoptosis in Infection written by Diane E. Griffin and published by Springer Science & Business Media. This book was released on 2005-08-29 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: (will follow)

The Baculoviruses

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Publisher : Springer
ISBN 13 : 9781489918352
Total Pages : 472 pages
Book Rating : 4.9/5 (183 download)

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Book Synopsis The Baculoviruses by : Lois K Miller

Download or read book The Baculoviruses written by Lois K Miller and published by Springer. This book was released on 2014-01-15 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:

BIOLOGY OF BACULOVIRUSES

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Publisher : Springer
ISBN 13 :
Total Pages : 294 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis BIOLOGY OF BACULOVIRUSES by : Robert R. Granados

Download or read book BIOLOGY OF BACULOVIRUSES written by Robert R. Granados and published by Springer. This book was released on 1986 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Viral Insecticides for Biological Control

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Publisher : Elsevier
ISBN 13 : 0323142370
Total Pages : 823 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Viral Insecticides for Biological Control by : Karl Maramorosch

Download or read book Viral Insecticides for Biological Control written by Karl Maramorosch and published by Elsevier. This book was released on 2012-12-02 with total page 823 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viral Insecticides for Biological Control focuses on the basic as well as applied aspects of viral insecticides, which have the potential to significantly reduce the current reliance on chemical pesticide technology. This book serves as a guide for the development of means to identify hazardous problems and prevent them. Organized into six parts with a total of 23 chapters, this book describes the taxonomy, nomenclature, identification, physical, biological, as well as chemical characteristics, replication, and pathology of insect viruses. This reference material also explores the dispersal, stability, and utilization of insect viruses as biological control agents. The factors and considerations that must be taken into account when a viral insecticide is sought as a large-scale commercial alternative to other more traditional methods of pest control are also addressed. Because this reference material collates information in this field of interest, it will benefit a wide audience of readers, including researchers, students, and those working directly in crop protection.

Industrial Scale Suspension Culture of Living Cells

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Publisher : John Wiley & Sons
ISBN 13 : 3527335471
Total Pages : 642 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Industrial Scale Suspension Culture of Living Cells by : Hans-Peter Meyer

Download or read book Industrial Scale Suspension Culture of Living Cells written by Hans-Peter Meyer and published by John Wiley & Sons. This book was released on 2014-08-04 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.