Author : Lianna Lee
Publisher :
ISBN 13 : 9780355763034
Total Pages : pages
Book Rating : 4.7/5 (63 download)
Book Synopsis The Effect of Activation on D-myo-inositol Phosphate Content in Almonds by : Lianna Lee
Download or read book The Effect of Activation on D-myo-inositol Phosphate Content in Almonds written by Lianna Lee and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Activated almonds are raw almonds that have been soaked in water for 12-24 hours at room temperature sometimes followed by a 24-hour drying period at low temperature (50 ̊C±5 ̊C). This treatment is thought to enhance the nutrient bioavailability of almonds by degrading nutrient inhibitors, such as phytic acid or D-myo-inositol hexakisphosphate (InsP6) through the release of phytase, or passive diffusion of InsP6 into the soaking medium. Over a wide pH range, InsP6 is a negatively charged compound that deters the absorption of essential nutrients by forming insoluble complexes with positively charged minerals (e.g. iron, zinc, and calcium). During soaking, almond seeds are subjected to conditions favorable for seed growth, and it is hypothesized that hydrating the seed triggers phytase and InsP6 degradation into lower myo-inositol phosphates (InsP) with less binding capacity. To monitor changes in InsP6 concentration, anion exchange chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was used to separate and quantify myo-inositol mono-, di, tris-, tetra-, penta- and hexakis-phosphate (InsP[subscript 1-6]). It was determined that almonds needed to be soaked for at least 24 hours at ambient temperature (24±2 ̊C) to reduce InsP6 content from 14.71 [mu]mol/g to 14.01 [mu]mol/g. The reduction in InsP6 after 24 hours of activation is statistically significant (p