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Download or read book The Development and Application of a Sequence-selective DNA Sensor written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Kelly M. Millan
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (13 download)
Download or read book The Development and Application of a Sequence-selective DNA Sensor written by Kelly M. Millan and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 960 pages
Book Rating : 4.F/5 ( download)
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2007 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Aik Teong Ooi
Publisher :
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.:/5 (659 download)
Download or read book Sequence-Specific DNA Detection Utilizing Custom-Designed Zinc Finger Proteins written by Aik Teong Ooi and published by . This book was released on with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA diagnostics are important technologies in molecular and cellular biology. By allowing identification of specific sequences, DNA-based diagnostics potentially provide more accurate and rapid results than protein- or antigen-based diagnostics, primarily because phenotypic changes come much later than changes in genotype. Despite this advantage, there are fewer diagnostic or imaging systems that target DNA than those targeting proteins, antibodies, or antigens. Each type of DNA-based diagnostic has its own, unique set of limitations; however, most can be attributed to issues related to sequence restriction, signal detection, specificity, or some combination thereof. For example, while PCR-based methods allow amplification and assessment of specific DNA sequences, they lack the ability to report information of specific cells, or cell types, within the heterogeneous pool of cells typically found in a tumor biopsy. In addition, none of the currently available DNA detection methods has the potential to be utilized in living cells, a disadvantage which limits the potential applications. The work presented here describes the design and development of a new methodology for the detection of specific double-stranded DNA sequences. This detection method is based on the concept that two inactive fragments of a reporter protein, each coupled to engineered zinc finger DNA-binding motifs, are able to reassemble and form an active complex in the presence of a predefined DNA sequence. This system, designated sequence-enabled reassembly (SEER), can achieve single base-pair specificity, and has the potential to be utilized in living cells. In this dissertation, we discuss the efforts from constructing to refining the system, as well as the future applications of SEER in diagnostics and therapeutics. Chapter I will provide an introduction to DNA detection methods, on which the principles of the SEER system are based. Chapter II describes the design and construction of an enzymatic SEER system, SEER-LAC, using beta-lactamase as the enzyme. In Chapter III, we outline the in vitro characterization of the SEER-LAC system, followed by its optimization in Chapter IV. Chapter V illustrates the efforts to develop SEER system for mammalian cell culture applications. In the final chapter, the future developments and applications of SEER are discussed.
Author : Anandhakumar Chandran
Publisher : Springer
ISBN 13 : 9811065470
Total Pages : 123 pages
Book Rating : 4.8/5 (11 download)
Download or read book Advancing Development of Synthetic Gene Regulators written by Anandhakumar Chandran and published by Springer. This book was released on 2017-09-19 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.
Author : Beata M. Kolakowski
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (11 download)
Download or read book Development of a Sequence-selective DNA Assay written by Beata M. Kolakowski and published by . This book was released on 1995 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Manoj Kumar Ram
Publisher : CRC Press
ISBN 13 : 1420005049
Total Pages : 390 pages
Book Rating : 4.4/5 (2 download)
Download or read book Sensors for Chemical and Biological Applications written by Manoj Kumar Ram and published by CRC Press. This book was released on 2018-10-03 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, sensor research has undergone a quiet revolution that will have a significant impact on a broad range of applications in areas such as health care, the environment, energy, food safety, national security, and manufacturing. Sensors for Chemical and Biological Applications discusses in detail the potential of chemical and biological sensors and examines how they are meeting the challenges of chem-bio terrorism by monitoring through enhanced specificity, fast response times, and the ability to determine multiple hazardous substances. Exploring the nanotechnology approach, and carrying this theme throughout the book, the chapters cover the sensing principles for, chemical, electrical, chromatographic, magnetic, biological, fluidic, optical, and ultrasonic and mass sensing systems. They address issues associated with cost, synthesis, and testing of new low cost materials with high sensitivity, selectivity, robustness, and speed for defined sensor applications. The book extensively discusses the detailed analysis of future impact of chemical and biological sensors in day-to-day life. Successful development of improved chemical sensor and biosensor systems and manufacturing procedures will not only increase the breadth and depth of the sensor industry, but will spill over into the design and manufacture of other types of sensors and devices that use nanofabrication and microfabrication techniques. This reference not only supplies versatile, hands-on tools useful in a broad array of disciplines, but also lays the interdisciplinary groundwork required for the achievement of sentient processing.
Author : Keith R. Mitchelson
Publisher : Elsevier
ISBN 13 : 0080471285
Total Pages : 399 pages
Book Rating : 4.0/5 (84 download)
Download or read book New High Throughput Technologies for DNA Sequencing and Genomics written by Keith R. Mitchelson and published by Elsevier. This book was released on 2011-09-22 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Sequencing and Genomics is the second volume in the Perspectives in Bioanalysis series, which looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century.Reviews of complementary developments in Sanger and SBS sequencing chemistries, capillary electrophoresis and microdevice integration, MS sequencing and applications set the framework for the book.* 'Hot Topic' with DNA sequencing continuing as a major research activity in many areas of life science and medicine.* Bringing together new developments in DNA sequencing technology* Reviewing issues relevant to the new applications used
Author : Eugene V. Koonin
Publisher : Springer Science & Business Media
ISBN 13 : 1475737831
Total Pages : 482 pages
Book Rating : 4.4/5 (757 download)
Download or read book Sequence — Evolution — Function written by Eugene V. Koonin and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequence - Evolution - Function is an introduction to the computational approaches that play a critical role in the emerging new branch of biology known as functional genomics. The book provides the reader with an understanding of the principles and approaches of functional genomics and of the potential and limitations of computational and experimental approaches to genome analysis. Sequence - Evolution - Function should help bridge the "digital divide" between biologists and computer scientists, allowing biologists to better grasp the peculiarities of the emerging field of Genome Biology and to learn how to benefit from the enormous amount of sequence data available in the public databases. The book is non-technical with respect to the computer methods for genome analysis and discusses these methods from the user's viewpoint, without addressing mathematical and algorithmic details. Prior practical familiarity with the basic methods for sequence analysis is a major advantage, but a reader without such experience will be able to use the book as an introduction to these methods. This book is perfect for introductory level courses in computational methods for comparative and functional genomics.
Author : Ajeet Kumar Kaushik
Publisher : CRC Press
ISBN 13 : 1315342200
Total Pages : 334 pages
Book Rating : 4.3/5 (153 download)
Download or read book Nanobiotechnology for Sensing Applications written by Ajeet Kumar Kaushik and published by CRC Press. This book was released on 2016-11-03 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the potential of nanosystems as a multidisciplinary science with the aim of the design and development of smart sensing technologies using micro/nano electrodes and novel nanosensing material. It discusses their integration with MEMS, miniaturized transduction systems, novel sensing strategies, and wearable sensors performing at POC for diagnostics and personalized health care monitoring. It presents basic concepts pertaining to nanobiosensor fabrication, developments in the field of smart nanomaterials, nano-enabling technologies, micro-nano hybrind platforms, and their applications in healthcare.
Author : Piet Herdewijn
Publisher : Springer Science & Business Media
ISBN 13 : 1592598234
Total Pages : 436 pages
Book Rating : 4.5/5 (925 download)
Download or read book Oligonucleotide Synthesis written by Piet Herdewijn and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Author : Anupama Rao Gular Srinivas
Publisher :
ISBN 13 :
Total Pages : 340 pages
Book Rating : 4.:/5 (865 download)
Download or read book Miniaturized Optical DNA Sensors Based on Conjugated Polymers written by Anupama Rao Gular Srinivas and published by . This book was released on 2013 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of highly sensitive and selective DNA sensors has fuelled applications in a wide range of fields including medical diagnostics, forensics, biodefense, food contamination and environment monitoring. Recently, conjugated polymers (CPs) have drawn attention as attractive novel materials for biosensors due to their tuneable optical and electronic properties and ability to respond to minor changes in their environment. The aim of this research was to develop miniaturised optical DNA sensors based on photoluminescent CPs. Investigations towards the effect of systematically substituting carboxylic acid functionalized CPs with polar methoxy, mono-ethylene glycol, di-ethylene glycol and tri-ethylene glycol sidechains on the solubility, optical and electrochemical properties were conducted. The resultant CPs were characterised using a range of techniques such as nuclear magnetic resonance (NMR), fourier infrared spectroscopy (FTIR), ultra-violet (UV-Vis) visible spectroscopy and fluorescence spectroscopy. Furthermore, three different novel optical DNA sensors on magnetic beads were designed and developed using the synthesized CPs. DNA hybridisation was monitored using fluorescence resonance energy transfer (FRET) and superquenching readout methodologies. These sensors exhibit desirable characteristics such as ease of handling (through magnetic deployment). All the three different sensing platforms were able to discriminate mismatches in the target sequences, theoretical detection limits to target DNA concentrations were calculated between 0.01 pM and 18.8 pM, improving resistance to non-specific interactions with DNA and proteins, fulfilling the requirements for a selective, sensitive, mobile, cost effective miniaturised DNA sensor.
Author : Nihan Aydemir
Publisher :
ISBN 13 :
Total Pages : 246 pages
Book Rating : 4.:/5 (957 download)
Download or read book Development of Highly Sensitive and Selective DNA Sensors Based on Conducting Polymers written by Nihan Aydemir and published by . This book was released on 2016 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sabu Thomas
Publisher : Elsevier
ISBN 13 : 0128236523
Total Pages : 634 pages
Book Rating : 4.1/5 (282 download)
Download or read book Nanosensors for Smart Manufacturing written by Sabu Thomas and published by Elsevier. This book was released on 2021-06-10 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanosensors for Smart Manufacturing provides information on the fundamental design concepts and emerging applications of nanosensors in smart manufacturing processes. In smart production, if the products and machines are integrated, embedded, or equipped with sensors, the system can immediately collect the current operating parameters, predict the product quality, and then feed back the optimal parameters to machines in the production line. In this regard, smart sensors and their wireless networks are important components of smart manufacturing. Nanomaterials-based sensors (nanosensors) offer several advantages over their microscale counterparts, including lower power consumption, fast response time, high sensitivity, lower concentration of analytes, and smaller interaction distance between sensors and products. With the support of artificial intelligence (AI) tools such as fuzzy logic, genetic algorithms, neural networks, and ambient intelligence, sensor systems have become smarter. This is an important reference source for materials scientists and engineers who want to learn more about how nanoscale sensors can enhance smart manufacturing techniques and processes. - Outlines the smart nanosensor classes used in manufacturing applications - Shows how nanosensors are being used to make more efficient manufacturing systems - Assesses the major obstacles to designing nanosensor-based manufacturing systems at an industrial scale
Author : Eugenia V. Buzaneva
Publisher : Springer Science & Business Media
ISBN 13 : 9781402021725
Total Pages : 502 pages
Book Rating : 4.0/5 (217 download)
Download or read book Frontiers of Multifunctional Integrated Nanosystems written by Eugenia V. Buzaneva and published by Springer Science & Business Media. This book was released on 2004-07-27 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the NATO Advanced Research Workshop, Illmenau, Germany from 12 to 16 July 2003
Author : Q. Y. Shu
Publisher : CABI
ISBN 13 : 9781780641027
Total Pages : 612 pages
Book Rating : 4.6/5 (41 download)
Download or read book Plant Mutation Breeding and Biotechnology written by Q. Y. Shu and published by CABI. This book was released on 2012 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive book covers the underlying scientific principles, state-of-the-art technologies and methodologies of plant mutagenesis. It covers historical development and commonly used terminologies, chemical and physical mutagenesis, mutation induction, mutation breeding and mutations in functional genomics research. Suitable both as a manual for professionals and a resource for students in plant breeding and research, the book includes exemplary cases of practical applications and an appendix of recommended doses of gamma and fast neutron irradiation for almost 200 plant species. It is
Author :
Publisher :
ISBN 13 :
Total Pages : 1422 pages
Book Rating : 4.:/5 (319 download)
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
