Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download Systems Biology Of Recombinant Protein Production By Fungi full books in PDF, epub, and Kindle. Read online Systems Biology Of Recombinant Protein Production By Fungi ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : Lifang Liu
Publisher :
ISBN 13 : 9789175970387
Total Pages : pages
Book Rating : 4.9/5 (73 download)
Download or read book Systems Biology of Recombinant Protein Production by Fungi written by Lifang Liu and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Eduardo A. Ceccarelli
Publisher : Frontiers E-books
ISBN 13 : 2889192946
Total Pages : 103 pages
Book Rating : 4.8/5 (891 download)
Download or read book Recombinant protein expression in microbial systems written by Eduardo A. Ceccarelli and published by Frontiers E-books. This book was released on 2014-10-02 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.
Author : Gerd Gellissen
Publisher : John Wiley & Sons
ISBN 13 : 3527604413
Total Pages : 429 pages
Book Rating : 4.5/5 (276 download)
Download or read book Production of Recombinant Proteins written by Gerd Gellissen and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
Author : Monika Schmoll
Publisher : Springer
ISBN 13 : 3319279513
Total Pages : 499 pages
Book Rating : 4.3/5 (192 download)
Download or read book Gene Expression Systems in Fungi: Advancements and Applications written by Monika Schmoll and published by Springer. This book was released on 2016-04-04 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology has emerged as one of the key environmentally safe technologies for the future which enables use of biomass to develop novel smart materials and to replace oil derived products. Fungi are the most efficient producers of the enzymes needed for this purpose and in addition they produce a plethora of secondary metabolites, among which novel antibiotics can be found. Industrial application and exploitation of the metabolic capacities of fungi requires highly productive and robust gene expression systems, which can be achieved by selection of appropriate species and strain improvement. In this book we aim to summarize homologous and heterologous gene expression systems of fungi for production of enzymes and secondary metabolites. A broad overview on requirements, challenges and successful applications shall serve as a basis for further development of fungi as biotechnological workhorses in research and industry.
Author : Varsha Gupta
Publisher : Springer
ISBN 13 : 9811008752
Total Pages : 527 pages
Book Rating : 4.8/5 (11 download)
Download or read book Basic and Applied Aspects of Biotechnology written by Varsha Gupta and published by Springer. This book was released on 2016-10-22 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.
Author : Paulina Balbas
Publisher : Springer Science & Business Media
ISBN 13 : 1592597742
Total Pages : 505 pages
Book Rating : 4.5/5 (925 download)
Download or read book Recombinant Gene Expression written by Paulina Balbas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Author : Otto-Wilhelm Merten
Publisher : Springer Science & Business Media
ISBN 13 : 9401597499
Total Pages : 396 pages
Book Rating : 4.4/5 (15 download)
Download or read book Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology written by Otto-Wilhelm Merten and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: More then 20 years have passed now since the first recombinant protein producing microorganisms have been developed. In the meanwhile, numerous proteins have been produced in bacteria, yeasts and filamentous fungi, as weIl as higher eukaryotic cells, and even entire plants and animals. Many recombinant proteins are on the market today, and some of them reached substantial market volumes. On the first sight one would expect the technology - including the physiology of the host strains - to be optimised in detail after a 20 year's period of development. However, several constraints have limited the incentive for optimisation, especially in the pharmaceutical industry like the urge to proceed quickly or the requirement to define the production parameters for registration early in the development phase. The additional expenses for registration of a new production strain often prohibits a change to an optimised strain. A continuous optimisation of the entire production process is not feasible for the same reasons.
Author : Brigitte Gasser
Publisher : Humana Press
ISBN 13 : 9781493990238
Total Pages : 431 pages
Book Rating : 4.9/5 (92 download)
Download or read book Recombinant Protein Production in Yeast written by Brigitte Gasser and published by Humana Press. This book was released on 2019-02-09 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Author : Roslyn M. Bill
Publisher : Humana Press
ISBN 13 : 9781493958719
Total Pages : 262 pages
Book Rating : 4.9/5 (587 download)
Download or read book Recombinant Protein Production in Yeast written by Roslyn M. Bill and published by Humana Press. This book was released on 2016-05-01 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews preparation of expression vectors, generation of high-yielding clones, scale-up, disruption of yeast cells to enable isolation of recombinant protein prior to purification and more, in the popular Methods in Molecular Biology format."
Author : Christoph Wittmann
Publisher : Springer
ISBN 13 : 3642138667
Total Pages : 166 pages
Book Rating : 4.6/5 (421 download)
Download or read book Biosystems Engineering II written by Christoph Wittmann and published by Springer. This book was released on 2010-08-18 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: -Morphology of Filamentous Fungi: Linking Cellular Biology to Process Engineering Using Aspergillus niger, By Rainer Krull, Christiana Cordes, Harald Horn, Ingo Kampen, Arno Kwade, Thomas R. Neu, and Bernd Nörtemann; -Multi-Scale Spatio-Temporal Modeling: Lifelines of Microorganisms in Bioreactors and Tracking Molecules in Cells, By Alexei Lapin, Michael Klann, and Matthias Reuss; -Impact of Profiling Technologies in the Understanding of Recombinant Protein Production, By Chandran Vijayendran and Erwin Flaschel -Engineering the Escherichia coli Fermentative Metabolism, By M. Orencio-Trejo, J. Utrilla, M.T. Fernàndez-Sandoval, G. Huerta-Beristain, G. Gosset, and A. Martinez; -Modeling Languages for Biochemical Network Simulation: Reaction vs Equation Based Approaches, By Wolfgang Wiechert, Stephan Noack, and Atya Elsheikh; -Impact of Thermodynamic Principles in Systems Biology, By J.J. Heijnen;
Author : Habib Driouch
Publisher : Cuvillier Verlag
ISBN 13 : 373693808X
Total Pages : 192 pages
Book Rating : 4.7/5 (369 download)
Download or read book Systems Biotechnology of Recombinant Protein Production in Aspergillus niger written by Habib Driouch and published by Cuvillier Verlag. This book was released on 2011-07-11 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The filamentous fungus Aspergillus niger is an important efficient microbial cell factory for industrial production of enzymes as well as organic acids or antibiotics. In submerged cultivation, A. niger exhibits a rather complex morphology which typically has a strong influence on production performance. Although difficult to control so far, the morphological shape is obviously linked to key production characteristics of fungal cell factories. In this regard, comprehensive approaches, combining systems wide analysis and optimization at the cellular level with process driven engineering of the bioreactor environment, seem most useful in order to achieve superior production processes. This was applied to recombinant proteins production in A. niger. The optimization included the use of talc or alumina micro particles, added to the culture, which allowed to the precise controlling the morphological shape of A. niger and increase enzyme production in different recombinant strains. Additionally, the targeted engineering of the morphology of A. niger into high-producing bio-pellet forms of various sizes by the addition of titanate micro particles was demonstrated. This strategy was combined with model-based medium design and development of efficient fed-batch strategies to optimize the production of the high-value enzyme fructofuranosidase, an important biocatalyst for neo-sugar in food or pharmaceutical industry, in the recombinant strain A. niger SKAn1015. As a result, the achieved enzyme titre could be increased to 2,800 U/mL, more than tenfold as compared to previously described processes. The enzyme, obtained by this micro particle enhanced process, could be applied as biocatalyst with minimal pre-treatment for the biosynthesis of 450 g/L of neo-sugar of the inulin type, such as 1 kestose and 1 nystose, which all display pre-biotics with substantial commercial interest. These studies were complementary by systems biotechnology analysis of A. niger as it is expected that this may lead to increased understanding of the context of cellular metabolism, regulation and thus further improvement of strains and processes. Fluxome analyses by 13C isotope studies and in silico design are applied to quantify the underlying carbon core metabolism of A. niger under different conditions. This yielding valuable insights towards the tailor made design of A. niger as a cell factory for recombinant protein production.
Author : Otto-Wilhelm Merten
Publisher : Springer Science & Business Media
ISBN 13 : 9780792371373
Total Pages : 434 pages
Book Rating : 4.3/5 (713 download)
Download or read book Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology written by Otto-Wilhelm Merten and published by Springer Science & Business Media. This book was released on 2001-11-30 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.
Author : André Damasio
Publisher : Frontiers Media SA
ISBN 13 : 2889630536
Total Pages : 125 pages
Book Rating : 4.8/5 (896 download)
Download or read book Advances in the Regulation and Production of Fungal Enzymes by Transcriptomics, Proteomics and Recombinant Strains Design written by André Damasio and published by Frontiers Media SA. This book was released on 2019-10-04 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several efforts have been made in developing strategies to supply the enzyme market, as well as in reducing its costs. It includes the selection of an appropriate enzyme source and the optimization of enzyme properties and secretion. Carbohydrate-Active Enzymes (CAZymes) are industrially relevant biocatalysts that are capable of degrading plant cell wall biomass. The most important secreted enzymes related to plant cell wall decomposition are cellulases, hemicellulases, and auxiliary enzymes. These enzymes have been applied in the hydrolysis of plant biomass for the production of second-generation (2G) ethanol and several other high added value products. One of the bottlenecks for 2G ethanol production is the cost of enzymes applied on plant biomass hydrolysis. The improvement of proteins production by fungi applying system biology and genetic engineering is an interesting and promising strategy to reduce the enzymes cost and make the 2G ethanol production viable. Fungi play an important role in plant biomass degradation and biotechnology by producing and secreting high yields of enzymes. In spite of the fact that filamentous fungi present several advantages compared to other microorganisms due to the high level of proteins production, heterologous protein production is far from optimal levels and still needs to be improved. Currently, heterologous production of certain proteins is generally considerably lower than the levels obtained to homologous production. Many strategies have been studied in order to improve heterologous production of proteins by filamentous fungi, including the deletion of genes that encode for proteases, the deletion of lectin-like ER-Golgi cargo receptors and the co-expression of specific chaperones. It has been shown that the main bottleneck in the production of heterologous proteins is not caused by the low expression of the target gene. An experimental evidence suggests that most target proteins produced in filamentous fungi are lost or stuck in the secretory pathway due to errors in processing, modification or misfolding that result in their elimination by endoplasmic reticulum (ER) quality control. Misfolded proteins alter homeostasis and proper ER functioning resulting in a state known as ER stress. ER stress activates a conserved signaling pathway called unfolded protein response (UPR) and ER-associated protein degradation (ERAD), which upregulates genes responsible for restoring protein folding homeostasis in the cell and degrades misfolded protein in the cytosol by the ubiquitin-proteasome system. The genetic manipulation of individual genes and changes in the genome seems not to be the best alternative to overcome the main bottlenecks in heterologous protein secretion. However, the understanding of complex interactions of important proteins and genes, as well as how they are regulated is more promising.
Author : Stefan Schillberg
Publisher : Humana
ISBN 13 : 9781071622407
Total Pages : 346 pages
Book Rating : 4.6/5 (224 download)
Download or read book Recombinant Proteins in Plants written by Stefan Schillberg and published by Humana. This book was released on 2022-05-27 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production. Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author : William B. O'Dell
Publisher : Elsevier
ISBN 13 : 0323901468
Total Pages : 460 pages
Book Rating : 4.3/5 (239 download)
Download or read book Recombinant Protein Expression: Prokaryotic Hosts and Cell-free Systems written by William B. O'Dell and published by Elsevier. This book was released on 2021-10-28 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starting a new recombinant protein production project in Escherichia coli -- From the notebook to recombinant protein production in Escherichia coli: Design of expression vectors and gene cloning -- Use of tandem affinity-buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins -- Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme -- Production of antibodies in SHuffle Escherichia coli strains -- Improved folding of recombinant protein via co-expression of exogenous chaperones -- Fusing an insoluble protein to GroEL apical domain enhances soluble expression in Escherichia coli -- Method for high-efficiency fed-batch cultures of recombinant Escherichia coli -- Fed-batch production of deuterated protein in Escherichia coli for neutron scattering experimentation -- Thermococcus kodakarensis provides a versatile hyperthermophilic archaeal platform for protein expression -- Recombinant protein expression in Sulfolobus islandicus -- High-level synthesis and secretion of laccase, a metalloenzyme biocatalyst, by the halophilic archaeon Haloferax volcanii -- Expression and tandem affinity purification of 20S proteasomes and other multisubunit complexes in Haloferax volcanii -- Purification and characterization of ribonucleoprotein effector complexes of Sulfolobus islandicus CRISPR-Cas systems -- Guidelines for nucleic acid template design for optimal cell-free protein synthesis using an Escherichia coli reconstituted system or a lysate-based system -- Cell-free protein synthesis of CRISPR ribonucleoproteins (RNP) -- Leishmania tarentolae cell-free based approach for rapid anitbody-antigen interaction analysis -- Cell-free protein synthesis using Chinese hamster ovary cells.
Author : Kevin Kavanagh
Publisher : John Wiley & Sons
ISBN 13 : 111997769X
Total Pages : 315 pages
Book Rating : 4.1/5 (199 download)
Download or read book Fungi written by Kevin Kavanagh and published by John Wiley & Sons. This book was released on 2011-08-04 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi: Biology and Applications, Second Edition provides a comprehensive treatment of fungi, covering biochemistry, genetics and the medical and economic significance of these organisms at introductory level. With no prior knowledge of the subject assumed, the opening chapters offer a broad overview of the basics of fungal biology, in particular the physiology and genetics of fungi and also a new chapter on the application of genomics to fungi. Later chapters move on to include more detailed coverage of topics such as antibiotic and chemical commodities from fungi, new chapters on biotechnological use of fungal enzymes and fungal proteomics, and fungal diseases of humans, antifungal agents for use in human therapy and fungal pathogens of plants.
Author : Joseph M. Fernandez
Publisher : Elsevier
ISBN 13 : 0080532357
Total Pages : 480 pages
Book Rating : 4.0/5 (85 download)
Download or read book Gene Expression Systems written by Joseph M. Fernandez and published by Elsevier. This book was released on 1998-12-21 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method
