Subattomole Amino Acid Analysis By Laser Induced Fluorescence

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Subattomole Amino Acid Analysis by Laser-Induced Fluorescence

Author : YF. Cheng
Publisher :
ISBN 13 :
Total Pages : 9 pages
Book Rating : 4.:/5 (125 download)

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Book Synopsis Subattomole Amino Acid Analysis by Laser-Induced Fluorescence by : YF. Cheng

Download or read book Subattomole Amino Acid Analysis by Laser-Induced Fluorescence written by YF. Cheng and published by . This book was released on 1990 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subattomole analysis of fluorescein isothiocyanate (FITC) derivatives of amino acids is produced by combining capillary zone electrophoresis for high-efficiency separation with laser-induced fluorescence for high sensitivity detection. To optimize the separation process, several buffer systems were investigated. A pH 10 carbonate buffer provided good separation of 14 FITC amino acids. Concentration detection limits range from 5 x 10-12M for alanine to 9 x 10-11M for lysine, injected onto the column; ~9 x 10-21 mol of alanine is contained within the ~1-nL injection volume at the detection limit. The alanine detection limit corresponds to less than 6000 molecules injected onto the column and represents a four order of magnitude improvement in the state of the art for fluorescent detection of amino acids and a six order of magnitude improvement in the state of the art for the detection limit for isothiocyanate derivatives of amino acids.

Capillary Electrophoresis in Biotechnology and Environmental Analysis

Author : Hasan Parvez
Publisher : CRC Press
ISBN 13 : 1466563699
Total Pages : 495 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Capillary Electrophoresis in Biotechnology and Environmental Analysis by : Hasan Parvez

Download or read book Capillary Electrophoresis in Biotechnology and Environmental Analysis written by Hasan Parvez and published by CRC Press. This book was released on 2023-01-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.

Handbook of Capillary Electrophoresis, Second Edition

Author : James P. Landers
Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)

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Book Synopsis Handbook of Capillary Electrophoresis, Second Edition by : James P. Landers

Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Capillary Gel Electrophoresis

Author : Andras Guttman
Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Capillary Electrophoresis

Author : Patrick Camilleri
Publisher : CRC Press
ISBN 13 : 9780849391279
Total Pages : 586 pages
Book Rating : 4.3/5 (912 download)

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Book Synopsis Capillary Electrophoresis by : Patrick Camilleri

Download or read book Capillary Electrophoresis written by Patrick Camilleri and published by CRC Press. This book was released on 1997-11-25 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.

Laser Techniques in Luminescence Spectroscopy

Author : Tuan Vo-Dinh
Publisher : ASTM International
ISBN 13 : 0803114559
Total Pages : 170 pages
Book Rating : 4.8/5 (31 download)

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Book Synopsis Laser Techniques in Luminescence Spectroscopy by : Tuan Vo-Dinh

Download or read book Laser Techniques in Luminescence Spectroscopy written by Tuan Vo-Dinh and published by ASTM International. This book was released on 1990 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Capillary Electrophoresis of Proteins and Peptides

Author : Mark A. Strege
Publisher : Springer Science & Business Media
ISBN 13 : 159259798X
Total Pages : 340 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Proteins and Peptides by : Mark A. Strege

Download or read book Capillary Electrophoresis of Proteins and Peptides written by Mark A. Strege and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.

Microcharacterization of Proteins

Author : Roland Kellner
Publisher : John Wiley & Sons
ISBN 13 : 3527613978
Total Pages : 346 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Microcharacterization of Proteins by : Roland Kellner

Download or read book Microcharacterization of Proteins written by Roland Kellner and published by John Wiley & Sons. This book was released on 2008-09-26 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics - the analysis of the whole set of proteins and their functions in a cell - is based on the revolutionary developments which have been achieved in protein analysis during the last years. The number of finished genome projects is growing and in parallel there is a dramatically increasing need to identify the products of revealed genes. Acting on a micro level modern protein chemistry increases our understanding of biological events by elucidating the relevant structure-function relationships. The second edition of the successful title Microcharacterization of Proteins presents a current overview of modern protein analysis: From sample preparation to sequence analysis, mass spectrometry and bioinformatics it informs about the tools needed in protein research. This makes the book indispensable for everyone involved in proteomics!

Quantitive Analysis Of Biospec

Author : Per Lundahl
Publisher : Routledge
ISBN 13 : 1317836545
Total Pages : 244 pages
Book Rating : 4.3/5 (178 download)

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Book Synopsis Quantitive Analysis Of Biospec by : Per Lundahl

Download or read book Quantitive Analysis Of Biospec written by Per Lundahl and published by Routledge. This book was released on 2021-09-01 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement of just a few hydrogen bonds can confer a remarkably specific reversible association between two molecules. A web of weak interactions governs biospecific recognition in general. The binding equilibria in living cells tune and coordinate a multitude of functions. The thermodynamic properties of such interactions are often studied by binding experiments in simplified and essentially ideal systems. However, similar types of studies may elucidate the biologically relevant dynamic steady-state conditions in living cells and organisms, allowing for the very wide range of interactant concentrations and the interplay between the many reactions and interactions. The development in biosciences will continue with in-depth studies of macromolecules and membranes. More detailed knowledge will allow analyses of delicate balances between substances and events in the complex systems involved in life processes. Methods to study biospecific affinities are thus highly important tools for understanding mechanisms and effects of molecular binding events in vivo and in vitro, e.g., in biochemical, biomedical and pharmaceutical research, and for biotechnological research and production.

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques

Author : James P. Landers
Publisher : CRC Press
ISBN 13 : 1420004956
Total Pages : 1598 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques by : James P. Landers

Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.

Bioanalytical Chemistry

Author : Susan R. Mikkelsen
Publisher : John Wiley & Sons
ISBN 13 : 1119057701
Total Pages : 492 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Bioanalytical Chemistry by : Susan R. Mikkelsen

Download or read book Bioanalytical Chemistry written by Susan R. Mikkelsen and published by John Wiley & Sons. This book was released on 2016-02-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry Provides an all in one approach for both beginners and experts, from a broad range of backgrounds, covering introductions, theory, advanced concepts and diverse applications for each method Each chapter progresses from basic concepts to applications involving real samples Includes three new chapters on Biomimetic Materials, Lab-on-Chip, and Analytical Methods Contains end-of-chapter problems and an appendix with selected answers

Chemical Weapons Convention Chemicals Analysis

Author : Markku Mesilaakso
Publisher : John Wiley & Sons
ISBN 13 : 0470854251
Total Pages : 476 pages
Book Rating : 4.4/5 (78 download)

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Book Synopsis Chemical Weapons Convention Chemicals Analysis by : Markku Mesilaakso

Download or read book Chemical Weapons Convention Chemicals Analysis written by Markku Mesilaakso and published by John Wiley & Sons. This book was released on 2005-12-13 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the procedures for collection of samples, sample preparation, and analysis of CWC-related chemicals. It deals with analytical procedures that can be followed in well-equipped off-site laboratories (designated laboratories), as well as the on-site analytical procedures that the OPCW inspectors use in sample collection and preliminary analysis of the samples in field conditions. A one-of-a-kind, highly topical handbook for every expert in the chemical weapons field Outlines the methods for analysing chemical weapons both on and off site Authored by international experts in the field from top laboratories in both government and academic institutions

Advanced Fluorescence Reporters in Chemistry and Biology I

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
ISBN 13 : 3642047025
Total Pages : 393 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis Advanced Fluorescence Reporters in Chemistry and Biology I by : Alexander P. Demchenko

Download or read book Advanced Fluorescence Reporters in Chemistry and Biology I written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2010-09-08 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence reporter is the key element of any sensing or imaging technology. Its optimal choice and implementation is very important for increasing the sensitivity, precision, multiplexing power, and also the spectral, temporal, and spatial reso- tion in different methods of research and practical analysis. Therefore, design of ?uorescence reporters with advanced properties is one of the most important problems. In this volume, top experts in this ?eld provide advanced knowledge on the design and properties of ?uorescent dyes. Organic dyes were the ?rst ?uorescent materials used for analytical purposes, and we observe that they retain their leading positions against strong competition of new materials – conjugated polymers, semiconductor nanocrystals, and metal chelating complexes. Recently, molecular and cellular biology got a valuable tool of organic ?uorophores synt- sized by cell machinery and incorporated into green ?uorescent protein and its analogs. Demands of various ?uorescence techniques operating in spectral, anisotropy, and time domains require focused design of ?uorescence reporters well adapted to these techniques. Near-IR spectral range becomes more and more attractive for various applications, and new dyes emitting in this range are strongly requested. Two-photonic ?uorescence has become one of the major tools in bioimaging, and ?uorescence reporters well adapted to this technique are in urgent need. These problems cannot be solved without the knowledge of fundamental principles of dye design and of physical phenomena behind their ?uorescence response.

Capillary Electrophoresis of Nucleic Acids

Author : Keith R. Mitchelson
Publisher : Springer Science & Business Media
ISBN 13 : 1592590551
Total Pages : 481 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

D-Amino Acids

Author : Tohru Yoshimura
Publisher : Springer
ISBN 13 : 4431560777
Total Pages : 352 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis D-Amino Acids by : Tohru Yoshimura

Download or read book D-Amino Acids written by Tohru Yoshimura and published by Springer. This book was released on 2016-08-25 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive overview of the roles of ᴅ-amino acids and latest research findings, to reveal their fascinating aspects and to facilitate better understanding of their important roles in physiology and diseases. It also provides useful hints for the development of drugs and functional foods. ᴅ-Amino acids were once called unnatural amino acids and were considered to be insignificant for eukaryotes especially in mammals. However, different ᴅ-amino acids have been revealed to be distributed in various mammalian tissues and to bear important physiological roles. For example, ᴅ-serine is implicated in memory formation and learning, and its abnormal concentration in tissues have been reported in neurological diseases such as schizophrenia and amyotrophic lateral sclerosis. ᴅ-Aspartate is found in a variety of mammalian tissues, particularly in the central nervous system and the genitals. ᴅ-Aspartate facilitates the endocrine secretion of prolactin, inhibits the secretion of melatonin, and plays a peculiar role in the control of reproductive functions in mammals, including the stimulation of testosterone synthesis. Written by the leading scientists in the field, this book is a valuable source of information for researchers in biochemistry, physiology, and neuroscience, as well as in the pharmaceutical and food industries.

Handbook of Capillary Electrophoresis Applications

Author : H. Shintani
Publisher : Springer Science & Business Media
ISBN 13 : 9400915616
Total Pages : 750 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Handbook of Capillary Electrophoresis Applications by : H. Shintani

Download or read book Handbook of Capillary Electrophoresis Applications written by H. Shintani and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 750 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

Analysis of Carbohydrates by Capillary Electrophoresis

Author : Aran Paulus
Publisher : Springer Science & Business Media
ISBN 13 : 332285020X
Total Pages : 192 pages
Book Rating : 4.3/5 (228 download)

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Book Synopsis Analysis of Carbohydrates by Capillary Electrophoresis by : Aran Paulus

Download or read book Analysis of Carbohydrates by Capillary Electrophoresis written by Aran Paulus and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wir sind umgeben von Kohlenhydraten: der süße Kaffee, Tee oder Dessert, die Stärke als Hauptkomponente unserer Nahrung und die Zellulose als Strukturelement in Pflanzen. Kohlenhydrate sind eine wichtige Klasse biologischer Moleküle, die an einer Anzahl wichtiger biochemischer Prozesse beteiligt sind. Gerade beginnen wir die Rolle von komplexen Zuckern zu verstehen, die an Proteine gebunden die Kommunikation von Zellen in einer "Zuckersprache" bewerkstelligen. Und nicht zuletzt kommen die ersten Kohlenhydratmoleküle als Medikamente auf den Markt. Anders als für andere Biopolymere sind die analytischen Methoden zur strukturellen Charakterisierung und Sequenzanalyse für Kohlenhydrate zur Zeit ungenügend, zum Teil wegen der überwältigenden Isotopenzahl der Zucker. Dieses Buch beschreibt die Entwicklung der letzten Jahre, die mit der Kapillarelektrophorese in Bezug auf eine miniaturisierte Analytik mit besserer Auflösung und Empfindlichkeit gemacht wurden. Instrumentierung, Derivatisierung, Trennbedingungen und Anwendungen in verschiedenen Disziplinen und Industrien wie z. B. Glykobiologie, Lebensmittelindustrie und Biotechnologie werden beschrieben.