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Structural Characterization And Separation Of Biomolecules By Matrix Assisted Laser Desorption Ionization Ion Mobility Mass Spectrometry
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Book Synopsis Structural Characterization and Separation of Biomolecules by Matrix-assisted Laser Desorption Ionization Ion-mobility Mass Spectrometry by : Ablatt Mahsut
Download or read book Structural Characterization and Separation of Biomolecules by Matrix-assisted Laser Desorption Ionization Ion-mobility Mass Spectrometry written by Ablatt Mahsut and published by . This book was released on 2009 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structural Separations of Endogenous and Exogenous Compounds Directly from Tissue Sections by Ion Mobility-mass Spectrometry by : Whitney Beth Parson
Download or read book Structural Separations of Endogenous and Exogenous Compounds Directly from Tissue Sections by Ion Mobility-mass Spectrometry written by Whitney Beth Parson and published by . This book was released on 2010 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry by : Bindesh Shrestha
Download or read book Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry written by Bindesh Shrestha and published by Elsevier. This book was released on 2021-04-23 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. Provides the introductory foundations of imaging mass spectrometry for those new to the technique Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work Includes case studies summarizing the imaging MS techniques developed for the class of molecules
Book Synopsis Analysis of Biomolecules by Matrix-assisted Laser Desorption/ionization-fourier Transform Mass Spectrometry by : Anissa Wai-Sinn Wong
Download or read book Analysis of Biomolecules by Matrix-assisted Laser Desorption/ionization-fourier Transform Mass Spectrometry written by Anissa Wai-Sinn Wong and published by . This book was released on 1998 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry by : Rainer Cramer
Download or read book Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry written by Rainer Cramer and published by Springer. This book was released on 2015-11-09 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics
Book Synopsis Advanced Fragmentation Methods in Biomolecular Mass Spectrometry by : Frederik Lermyte
Download or read book Advanced Fragmentation Methods in Biomolecular Mass Spectrometry written by Frederik Lermyte and published by Royal Society of Chemistry. This book was released on 2020-12-11 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: Breaking down large biomolecules into fragments in a controlled manner is key to modern biomolecular mass spectrometry. This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods. It provides a comprehensive overview of their history, mechanisms, instrumentation, and key applications. With contributions from leading experts, this book will act as an authoritative guide to these methods. Aimed at postgraduate and professional researchers, mainly in academia, but also in industry, it can be used as supplementary reading for advanced students on mass spectrometry or analytical (bio)chemistry courses.
Book Synopsis Application of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry to the Study of Bacterial Biomolecules by : Teresa Catherine Cain
Download or read book Application of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry to the Study of Bacterial Biomolecules written by Teresa Catherine Cain and published by . This book was released on 1997 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Structural Analysis of Macromolecular Complexes Using Electrospray Ionization Mass Spectrometry Based Approaches by : Jingshu Guo
Download or read book Structural Analysis of Macromolecular Complexes Using Electrospray Ionization Mass Spectrometry Based Approaches written by Jingshu Guo and published by . This book was released on 2013 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) has become one of the most robust, reliable and widely used analytical techniques in scientific research due to factors like its speed of analysis, ease of operation, high sensitivity, and applicability to a broad range of analytes. The development of the so-called "soft" ionization methods electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) played a pivotal role in the application of MS to biochemical and biophysical fields enabling comprehensive studies of biomolecules, macromolecules and their complexes. This dissertation presents method development and the application of MS-based strategies for complete sequence and structure determination of hemoglobin (Hb); investigation of the interaction between human Hb and Band 3; characterization of the interaction between Alanyl-tRNA synthetase with tRNA and various ligands; and characterization of various thiol-protected silver nanoclusters. First, the complete primary amino acid sequences of hemoglobin from the endangered species snow leopard (Uncia uncia), Amur tiger (Panthera tigris altaica), Cheetah (Acinonyx jubatus) and Francois' Langur (Trachypithecus francoisi) were determined using a combination of the bottom-up approach to proteomics in combination with single crystal X-ray diffraction. In this approach, MS was used to provide on average 70% coverage of the sequences, which was used in refinement of the diffraction data. The electron density maps from the diffraction data were used to determine the parts of the sequences not covered by MS as well as differentiate between isomeric residues. During the refinement of the X-ray diffraction data, a unique conformational state, the Bform, for naturally occurring ligated hemoglobin was discovered in two feline Hbs. Structural comparisons and possible biological relevance for the B-form will be presented. The binding of human hemoglobin to synthetic peptides corresponding to the Hb-binding sites of human erythrocyte Band 3 is also presented. The effects on binding of ligation and oxidation state of normal human HbA; human sickle HbS; Hb-binding site and peptide length; and Band 3 N-terminal acetylation were investigated. The optimization of MS instrumental parameters and solution conditions for stabilizing and protecting labile thiol-protected silver nanoclusters through ionization, mass analysis and detection is presented. The relatively much more stable and well characterized gold-glutathione cluster Au25(SG)18 was used as a model for instrumental optimization (SG, glutathione). The most crucial instrumental parameters to protecting the clusters were cone gas flow rate, trap/transfer collision energy, and source temperature. Using these optimized parameters and adjustment of solution conditions led to the formula assignment for Ag32(SG)19, the species isolated from band 6 of a polyacrylamide gel electrophoretic separation. Using a similar approach the formula assignment for Ag44L30-4 (L, p-mercaptobenzoic acid) was confirmed. Collision induced dissociation (CID) studies were used to demonstrate that Ag43L28-3, another abundant species in the mass spectra was in fact a fragment of the intact cluster from the facile loss of AgL2-. CID and ion mobility mass spectrometry (IMMS) of Au25(SG)18, Ag32(SG)19, and Ag44L30-4 were used to gain insight into the surface structure and stability of the fragile silver clusters. Finally, the 3'-tRNA binding site on E. coli Alanyl-tRNA synthetase was identified by first crosslinking the 3'-periodate-oxidized tRNAala to the enzyme followed by the bottom-up MS sequencing. Modified lysine residues identified were 74, 526 585, 637, 739 and 648. Lys 74 was in the aminoacylation domain while 526 585, 637, 739 and 648 were in the editing domain. These results highlight an alteration of the 3'-terminal of tRNAala interaction with the enzyme. To study the stability ARS with various ligands, non-hydrolysable adenylate analogs, ASAd and GSAd, were used. Data shows that complete binding of ASAd and GSAd was achieved and that binding occurred in a 1:1 stoichiometry. Binding of ASAd, the analog of the cognate ligand, stabilizes the enzyme against changes in solution pH relative to the apo-enzyme and the enzyme with GSAd bound.
Book Synopsis Matrix Assisted Laser Desorption Ionization with External Source Quadrupole Fourier Transform Mass Spectrometry for the Analysis of Biomolecules by : Steven Travis Fannin
Download or read book Matrix Assisted Laser Desorption Ionization with External Source Quadrupole Fourier Transform Mass Spectrometry for the Analysis of Biomolecules written by Steven Travis Fannin and published by . This book was released on 1995 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis New Methods for the Study of Biomolecular Complexes by : W. Ens
Download or read book New Methods for the Study of Biomolecular Complexes written by W. Ens and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: A NATO Advanced Research Workshop entitled New Methods for the Study of Molecular Aggregates was held at Tbe Lodge at Kananaskis Village, Alberta, Canada from 16 -20 June 1996. In fact the meeting was entirely concerned with the problem of analyzing biomolecular complexes, so the title of these proceedings has been altered to give a more precise description of the content. Tbe workshop was hosted by the time-of-flight group of the Department of Physics at the University of Manitoba, and was attended by 64 participants from around the world. '!\venty-one invited talks were given and 27 papers were presented as posters. Of the 48 contributions, 22 papers (12 orals, 10 posters) are included in these proceedings. Tbe subject of the conference was the investigation of noncovalent biomolecular complexes, with particular focus on the application of mass spectrometry to their characterization. '!\vo new ionization techniques introduced in the late 1980s, electrospray ionization (ES I) and matrix-assisted laser desorptionlionization (MALDI), resulted in a breakthrough in mass spectrometry, enabling its use in molecular weight and primary structure determination of biopolymers larger than 100 kDa. Recently it has been discovered that ESI mass spectrometry mayaiso be used to characterize complexes containing noncovalent interactions, thus opening new perspectives for supramolecular chemistry. ESI mass spectrometry has the advantage that the sampie is introduced from a homogenous solution which can be maintained at near physiological conditions of pR, concentration, and temperature.
Book Synopsis Analysis of Biomolecules Using Matrix-assisted Laser Desorption/ionization Mass Spectrometry by : Jennifer Ann Firestine
Download or read book Analysis of Biomolecules Using Matrix-assisted Laser Desorption/ionization Mass Spectrometry written by Jennifer Ann Firestine and published by . This book was released on 2000 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Laser Desorption/ionization Mass Spectrometry for Direct Profiling and Imaging of Small Molecules from Raw Biological Materials by :
Download or read book Laser Desorption/ionization Mass Spectrometry for Direct Profiling and Imaging of Small Molecules from Raw Biological Materials written by and published by . This book was released on 2008 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matrix-assisted laser desorption/ionization(MALDI) mass spectrometry(MS) has been widely used for analysis of biological molecules, especially macromolecules such as proteins. However, MALDI MS has a problem in small molecule (less than 1 kDa) analysis because of the signal saturation by organic matrixes in the low mass region. In imaging MS (IMS), inhomogeneous surface formation due to the co-crystallization process by organic MALDI matrixes limits the spatial resolution of the mass spectral image. Therefore, to make laser desorption/ionization (LDI) MS more suitable for mass spectral profiling and imaging of small molecules directly from raw biological tissues, LDI MS protocols with various alternative assisting materials were developed and applied to many biological systems of interest. Colloidal graphite was used as a matrix for IMS of small molecules for the first time and methodologies for analyses of small metabolites in rat brain tissues, fruits, and plant tissues were developed. With rat brain tissues, the signal enhancement for cerebroside species by colloidal graphite was observed and images of cerebrosides were successfully generated by IMS. In addition, separation of isobaric lipid ions was performed by imaging tandem MS. Directly from Arabidopsis flowers, flavonoids were successfully profiled and heterogeneous distribution of flavonoids in petals was observed for the first time by graphite-assisted LDI(GALDI) IMS.
Book Synopsis Analyzing Biomolecular Interactions by Mass Spectrometry by : Jeroen Kool
Download or read book Analyzing Biomolecular Interactions by Mass Spectrometry written by Jeroen Kool and published by John Wiley & Sons. This book was released on 2015-05-04 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
Book Synopsis Matrix-assisted Laser Desorption/ionization Mass Spectrometric Study of Biologic Molecules by : Yan-Hui Liu
Download or read book Matrix-assisted Laser Desorption/ionization Mass Spectrometric Study of Biologic Molecules written by Yan-Hui Liu and published by . This book was released on 1996 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Laser Desorption Ionization Mass Spectrometry Based on Nanophotonic Structure: From Material Design to Mechanistic Understanding by : Moon-Ju Kim
Download or read book Laser Desorption Ionization Mass Spectrometry Based on Nanophotonic Structure: From Material Design to Mechanistic Understanding written by Moon-Ju Kim and published by Springer Nature. This book was released on 2023-11-11 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents innovative laser desorption ionization (LDI)-active nanophotonic structures for addressing the challenges that matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is currently facing and for enhancing LDI efficiency. It presents a variety of cutting-edge nanophotonic structures to satisfy the mass-analytical needs of sensitivity, reproducibility, and quantification. As opposed to the commercialized, conventional organic matrix used in MALDI-MS, these nanostructures are validated to be highly effective in detecting small metabolites and drugs, highlighting their considerable potential in the mass spectrometry field. It also systematically elucidates fundamental LDI processes in terms of the photo-thermal, electronic, and structural characteristics of nanophotonic structures, offering mechanistic knowledge of LDI-MS. Even though LDI-MS performance is heavily influenced by a number of nanostructure parameters, relatively little is known about the LDI processes associated with those characteristics. An in-depth understanding of nanostructure characteristics and LDI mechanisms thus paves the way for more effective, rational design and development of nanostructures with improved LDI capabilities. Further, with a focus on multiple cascades in nanostructure functions in response to laser pulse stimuli, this book provides detailed, step-by-step procedures to design and construct a nanophotonic, LDI-active platform, which may serve as a roadmap for graduate students in the field of mass spectrometry. Readers, including graduate students, researchers, and experts working in the related areas of mass spectrometry, nanophotonics, and material science and material engineering, will find a wealth of useful information in this book.
Book Synopsis Enhanced Separations Strategies for Complex Sample Characterization Using Ion Mobility-mass Spectrometry by : J. Rafael Montenegro Burke
Download or read book Enhanced Separations Strategies for Complex Sample Characterization Using Ion Mobility-mass Spectrometry written by J. Rafael Montenegro Burke and published by . This book was released on 2016 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plasma Chromatography by : T.W. Carr
Download or read book Plasma Chromatography written by T.W. Carr and published by Springer. This book was released on 1984-03-31 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
