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Small Angle X Ray Scattering From Biological Macromolecules And Nanoparticles
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Book Synopsis Small Angle X-ray Scattering from Biological Macromolecules and Nanoparticles by :
Download or read book Small Angle X-ray Scattering from Biological Macromolecules and Nanoparticles written by and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Small-angle x-ray scattering (SAXS) is experimencing a renaisance in the studies of macromolecular solutions, allowing one to study the structure of native particles and to rapidly analyze structural changes in response to variations in external conditions. Novel data analysis methods, significantly enhanced resolution, and reliability of structural models are provided by the technique. Emerging automation of the experiment, data processing, and data interpretation make SAXS a streamlined tool for large-scale structural studies in molecular biology. The method provides low-resolution macromolecular shapes ab initio and is readily combined with other structural and biochemical techniques in multidisciplinary studies to build rigid body models of complexes and to characterize oligomeric mixtures and flexible systems. The novel approaches also became useful for non-biological systems and processes, e.g., the formation of nanoparticles.
Book Synopsis Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules by : Dmitri I. Svergun
Download or read book Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules written by Dmitri I. Svergun and published by OUP Oxford. This book was released on 2013-08-08 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Small-angle scattering of X-rays (SAXS) and neutrons (SANS) is an established method for the structural characterization of biological objects in a broad size range from individual macromolecules (proteins, nucleic acids, lipids) to large macromolecular complexes. SAXS/SANS is complementary to the high resolution methods of X-ray crystallography and nuclear magnetic resonance, allowing for hybrid modeling and also accounting for available biophysical and biochemical data. Quantitative characterization of flexible macromolecular systems and mixtures has recently become possible. SAXS/SANS measurements can be easily performed in different conditions by adding ligands or binding partners, and by changing physical and/or chemical characteristics of the solvent to provide information on the structural responses. The technique provides kinetic information about processes like folding and assembly and also allows one to analyze macromolecular interactions. The major factors promoting the increasingly active use of SAXS/SANS are modern high brilliance X-ray and neutron sources, novel data analysis methods, and automation of the experiment, data processing and interpretation. In this book, following the presentation of the basics of scattering from isotropic macromolecular solutions, modern instrumentation, experimental practice and advanced analysis techniques are explained. Advantages of X-rays (rapid data collection, small sample volumes) and of neutrons (contrast variation by hydrogen/deuterium exchange) are specifically highlighted. Examples of applications of the technique to different macromolecular systems are considered with specific emphasis on the synergistic use of SAXS/SANS with other structural, biophysical and computational techniques.
Book Synopsis Small Angle X-Ray Scattering from Biological Macromolecules by : Michael Reich
Download or read book Small Angle X-Ray Scattering from Biological Macromolecules written by Michael Reich and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biological Small Angle Scattering by : Eaton E. Lattman
Download or read book Biological Small Angle Scattering written by Eaton E. Lattman and published by Oxford University Press. This book was released on 2018-07-04 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Small angle solution scattering (SAS) is increasingly being applied to biological problems. It is a complementary technique that, when applied in appropriate circumstances with carefully structured questions, can provide unique information not available from other techniques. While small angle solution scattering has been around for some time, a confluence of recent developments has dramatically enhanced its power. Intense third generation X-ray sources, low noise detectors, development of new algorithms and the computational power to take advantage of these have all matured, and use of free-electron x-ray laser sources is on the horizon. Whole new classes of experiments and analyses have been created as a result. These include the generation of molecular envelopes, the ability to do time-resolved studies, and the ability to account for structural changes using modelling based on the SAS data. The technical improvements have also reduced the amount of time and material needed to carry out an experiment. Beamtime at synchrotron sources is in demand, workshops on the subject are popular and researchers adopting the technique as part of their repertoire are growing. With these in mind, this book was written to guide structural biologists who may wish to adopt the technique, understand its strengths and weaknesses or just have a general interest in its potential.
Book Synopsis Small Angle Scattering Part A: Methods for Structural Investigation by :
Download or read book Small Angle Scattering Part A: Methods for Structural Investigation written by and published by Elsevier. This book was released on 2022-10-15 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Small Angle Scattering: Methods for Structural Investigation
Book Synopsis Joint Use of Small Angle X-ray Scattering with High Resolution Methods to Study Flexible Biological Macromolecules by :
Download or read book Joint Use of Small Angle X-ray Scattering with High Resolution Methods to Study Flexible Biological Macromolecules written by and published by . This book was released on 2014 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Small-Angle X-ray Scattering (SAXS) in solution is an established technique for the structural characterization of biological macromolecules. SAXS allows one to study proteins, nucleic acids and their complexes in near physiological environments. olecules. The improvements described in this work, mainly devoted to molecular modelling and data analysis, shall overcome many of the limitations encountered when SAXS is employed to assess flexibility. At a more general level, this dissertation aims at encouraging the use of a hybrid approach as standard modus operandi in the field of structural biology where detailed atomic observations - offered by high resolution techniques - are synergistically combined with the overall picture offered by SAXS in solution.
Book Synopsis X-ray Scattering by : Alicia Esther Ares
Download or read book X-ray Scattering written by Alicia Esther Ares and published by BoD – Books on Demand. This book was released on 2017-01-25 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray scattering techniques are a family of nondestructive analytical techniques. Using these techniques, scientists obtain information about the crystal structure and chemical and physical properties of materials. Nowadays, different techniques are based on observing the scattered intensity of an X-ray beam hitting a sample as a function of incident and scattered angle, polarization, and wavelength. This book is intended to give overviews of the relevant X-ray scattering techniques, particularly about inelastic X-ray scattering, elastic scattering, grazing-incidence small-angle X-ray scattering, small-angle X-ray scattering, and high-resolution X-ray diffraction, and, finally, applications of X-ray spectroscopy to study different biological systems.
Book Synopsis Handbook of Materials Characterization by : Surender Kumar Sharma
Download or read book Handbook of Materials Characterization written by Surender Kumar Sharma and published by Springer. This book was released on 2018-09-18 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the widely used experimental techniques available for the structural, morphological, and spectroscopic characterization of materials. Recent developments in a wide range of experimental techniques and their application to the quantification of materials properties are an essential side of this book. Moreover, it provides concise but thorough coverage of the practical and theoretical aspects of the analytical techniques used to characterize a wide variety of functional nanomaterials. The book provides an overview of widely used characterization techniques for a broad audience: from beginners and graduate students, to advanced specialists in both academia and industry.
Book Synopsis Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules by : Dmitriĭ Ivanovich Svergun
Download or read book Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules written by Dmitriĭ Ivanovich Svergun and published by . This book was released on 2013-08-08 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes all aspects of the technique of small-angle scattering of X-rays and neutrons, including instrumentation, sample requirements, data interpretation and modelling methods, in a comprehensive way and gives examples of applications in various fields of biophysics and biochemistry.
Book Synopsis Semiconductor Nanocrystals by : Alexander L. Efros
Download or read book Semiconductor Nanocrystals written by Alexander L. Efros and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: A physics book that covers the optical properties of quantum-confined semiconductor nanostructures from both the theoretical and experimental points of view together with technological applications. Topics to be reviewed include quantum confinement effects in semiconductors, optical adsorption and emission properties of group IV, III-V, II-VI semiconductors, deep-etched and self assembled quantum dots, nanoclusters, and laser applications in optoelectronics.
Book Synopsis Membrane Protein Crystallization by :
Download or read book Membrane Protein Crystallization written by and published by Academic Press. This book was released on 2009-05-29 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.
Book Synopsis Biological Small Angle Scattering: Techniques, Strategies and Tips by : Barnali Chaudhuri
Download or read book Biological Small Angle Scattering: Techniques, Strategies and Tips written by Barnali Chaudhuri and published by Springer. This book was released on 2017-12-07 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.
Book Synopsis Small Angle Scattering Part A: Methods for Structural Investigation by :
Download or read book Small Angle Scattering Part A: Methods for Structural Investigation written by and published by Academic Press. This book was released on 2022-11-19 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Small Angle Scattering: Methods for Structural Investigation
Book Synopsis Measuring Biological Impacts of Nanomaterials by : Joachim Wegener
Download or read book Measuring Biological Impacts of Nanomaterials written by Joachim Wegener and published by Springer. This book was released on 2016-02-02 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews several aspects of the biological response to nanoscale particles on a molecular and cellular level. Nanoscale materials and nanoscale particles in particular have interesting properties and beneficial applications. While they thus have entered our daily lifes on many different levels (from electronics, over textiles, packaging or surface modifications, to biomedical applications), general rules describing their interaction with biological structures and biological matter are still difficult to derive. The existing literature suggests a variety of interaction schemes between nanoparticles and biological objects, not dispelling the public concerns about possible health effects and harmful properties. A systematic approach to the problem is needed and timely. This book specifically emphasizes bioanalytical problems starting from the characterization of the nanomaterials to the pitfalls and potential artifacts of state-of-the-art cytotoxicity assays that are frequently used to study harmful effects on cells. It also highlights the application of label-free bioanalytical techniques that can potentially complement the present approaches and hence provide new perspectives on this highly discussed cutting-edge field of research and public concern.
Book Synopsis Small-angle X-ray Scattering by : Harry Brumberger
Download or read book Small-angle X-ray Scattering written by Harry Brumberger and published by . This book was released on 1967 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Engineering, Medicine and Science at the Nano-Scale by : Stephen J. Fonash
Download or read book Engineering, Medicine and Science at the Nano-Scale written by Stephen J. Fonash and published by John Wiley & Sons. This book was released on 2018-08-17 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Students at universities the world over will benefit from the authors' concise treatment, arising out of lectures given for a graduate and advanced undergraduate course at Penn State University (USA) and University of Technology Delft (NL). The textbook begins by addressing, in general terms, the phenomena and peculiarities that occur at the nanoscale. In the following five chapters, readers are introduced in detail to nanoscale physics, chemistry, materials science, and biology, followed by chapters on synthesis and fabrication as well as characterization at the nanoscale. In the next four chapters a variety of exemplary applications taken from a wide range of sectors are also presented and discussed. Concerns for safety, environmental impact, workforce development, economic wellbeing, and societal change issues arising from nanotechnology are woven throughout the book and additionally form the focus of the last two chapters.
Book Synopsis Scattering Studies of Polymeric Micelles and Biological Macromolecules by : Manja Annette Behrens
Download or read book Scattering Studies of Polymeric Micelles and Biological Macromolecules written by Manja Annette Behrens and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
